RchiOBHm_Chr6g0306361

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000672
Physical Location & Seq
Forward (+)
65095340 .. 65096822
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27538

Sequence Viewer

Length: 756 bp
ATGACCACCATATTTGAGAGACGTGAATTTAACACCGGCGTTTATCAAAGGTATGAAAACAGAATGTTCCGAAGCAAATCCTGCGTGGACGTACTCCCGATCAAATTCTTCCGGTGGAAGACATATGCAGACCATAACCCAGATATCCAAGATTTTGACGCTGAACCAGCAACTTTCTTATTTTCAAAAGTTCTCAAGTTGCGGTTCTCGAGGGAGAGGGCAAGTGGAAATGCAAAGACAACGATGGAGAGACTTGCTAGGTATCAAAGGGTCATAGCTGGGTCTCCCAGGGAAGCAAACAAGTTAGAGAACAAGTTGGGTAGGTACCTTACCACAACTTTGGGAATTCACGAAGATGATCAACCGTTTACTATTGTAAAAATAATGGAAATGCTCCGAAAAGCCGTCCCCGATAAAGTTATTGAAGTACACATGCTGGAAATGACATTTAGAAGTACGAATTGGTCCTTTCTGTGGGGCTTCGACGAGGATGGGGACGATATATACTACAGGGCTAAGCTGGTTCCTGCTCCGAAATCATTCATCGAGTCTCTGGAGGAAGTGAGACTACTTGATGATGATGATAATCTGGAATTGCTTGGATTGGAGTGTCCTATTTGTATGCAGGTGTTTGCAGATACCAGCATTACTCGGTTGCCATGCTCACACTATTACCATCGAGATTGCATTGTCCAGTGGCTCCAGATCAATCATGTCTGTCCCACTTGTCGACACCCTAATGCTCCGATGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

251

Amino Acids

29.54

Weight (kDa)

6.4

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 203 - 244 4.9e-13 Ring finger domain
zf-C3HC4_2 PF13923 203 - 243 4.6e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 203 - 230 1.1e-07 RING-like zinc finger
zf-C3HC4 PF00097 204 - 243 1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 204 - 244 1.8e-06 RING-H2 zinc finger domain
zf-RING_UBOX PF13445 204 - 241 2.2e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 616
Acc36I ACCTGC 1 cut(s) 616
Acc65I GGTACC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 730
AciI CCGC 1 cut(s) 202
AcsI RAATTY 3 cut(s) 26, 104, 345
AfaI GTAC 4 cut(s) 93, 326, 429, 457
AfiI CCNNNNNNNGG 1 cut(s) 474
AgsI TTSAA 2 cut(s) 186, 425
AjiI CACGTC 1 cut(s) 23
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 2 cut(s) 278, 520
AluI AGCT 2 cut(s) 278, 520
Alw26I GTCTC 5 cut(s) 13, 244, 288, 555, 559
Ama87I CYCGRG 1 cut(s) 208
ApoI RAATTY 3 cut(s) 26, 104, 345
Asp700I GAANNNNTTC 1 cut(s) 539
Asp718I GGTACC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 465
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 1 cut(s) 465
BanI GGYRCC 1 cut(s) 324
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
BbsI GAAGAC 1 cut(s) 125
BccI CCATC 3 cut(s) 238, 485, 684
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 1 cut(s) 289
BclI TGATCA 1 cut(s) 358
BcoDI GTCTC 5 cut(s) 13, 244, 288, 555, 559
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 508
BfuAI ACCTGC 1 cut(s) 616
BlpI GCTNAGC 1 cut(s) 516
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 465
BmeT110I CYCGRG 1 cut(s) 208
BmgBI CACGTC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 465
BmiI GGNNCC 3 cut(s) 326, 525, 701
BmrFI CCNGG 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 125
BpmI CTGGAG 2 cut(s) 575, 686
Bpu1102I GCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 179
BsaBI GATNNNNATC 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 287, 288
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 694
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 287, 288
BseGI GGATG 1 cut(s) 496
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 694
BseYI CCCAGC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 324
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 2 cut(s) 36, 112
BslFI GGGAC 3 cut(s) 392, 509, 705
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 5 cut(s) 13, 244, 288, 555, 559
BsmBI CGTCTC 1 cut(s) 13
BsmFI GGGAC 3 cut(s) 392, 509, 705
Bso31I GGTCTC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 208
Bsp143I GATC 3 cut(s) 99, 358, 705
Bsp1720I GCTNAGC 1 cut(s) 516
BspACI CCGC 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 326, 525, 701
BspMI ACCTGC 1 cut(s) 616
BspT107I GGYRCC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 694
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 287, 288
BssMI GATC 3 cut(s) 99, 358, 705
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 366
BstAPI GCANNNNNTGC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 516
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 3 cut(s) 102, 361, 708
BstMAI GTCTC 5 cut(s) 13, 244, 288, 555, 559
BstMBI GATC 3 cut(s) 99, 358, 705
BstMWI GCNNNNNNNGC 2 cut(s) 81, 167
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 287
BstSFI CTRYAG 1 cut(s) 508
BstV2I GAAGAC 1 cut(s) 125
BstXI CCANNNNNNTGG 1 cut(s) 340
BtrI CACGTC 1 cut(s) 23
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 1 cut(s) 701
BveI ACCTGC 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 465
CseI GACGC 1 cut(s) 167
Csp6I GTAC 4 cut(s) 92, 325, 428, 456
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 3 cut(s) 433, 660, 713
CviJI RGCY 6 cut(s) 278, 404, 480, 515, 520, 700
CviKI_1 RGCY 6 cut(s) 278, 404, 480, 515, 520, 700
CviQI GTAC 4 cut(s) 92, 325, 428, 456
DdeI CTNAG 1 cut(s) 516
DpnI GATC 3 cut(s) 101, 360, 707
DpnII GATC 3 cut(s) 99, 358, 705
Eco31I GGTCTC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 287
EcoRV GATATC 1 cut(s) 145
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 3 cut(s) 436, 663, 716
FaqI GGGAC 3 cut(s) 392, 509, 705
FatI CATG 3 cut(s) 432, 659, 712
FauNDI CATATG 1 cut(s) 124
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 1 cut(s) 730
FokI GGATG 1 cut(s) 503
FspBI CTAG 1 cut(s) 258
GsaI CCCAGC 1 cut(s) 282
GsuI CTGGAG 2 cut(s) 575, 686
HapII CCGG 2 cut(s) 36, 112
HgaI GACGC 1 cut(s) 167
Hin1II CATG 3 cut(s) 436, 663, 716
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HinfI GANTC 1 cut(s) 548
HpaII CCGG 2 cut(s) 36, 112
Hpy166II GTNNAC 4 cut(s) 88, 369, 430, 731
Hpy188I TCNGA 4 cut(s) 71, 398, 534, 747
Hpy188III TCNNGA 7 cut(s) 97, 208, 350, 554, 590, 680, 703
Hpy8I GTNNAC 4 cut(s) 88, 369, 430, 731
Hpy99I CGWCG 1 cut(s) 488
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4IV ACGT 2 cut(s) 22, 90
HpyCH4V TGCA 5 cut(s) 128, 233, 625, 635, 687
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 167
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 2 cut(s) 22, 90
Hsp92II CATG 3 cut(s) 436, 663, 716
KpnI GGTACC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 3 cut(s) 99, 358, 705
LmnI GCTCC 4 cut(s) 399, 535, 705, 748
MaeI CTAG 1 cut(s) 258
MaeII ACGT 2 cut(s) 22, 90
MalI GATC 3 cut(s) 101, 360, 707
MboI GATC 3 cut(s) 99, 358, 705
MboII GAAGA 3 cut(s) 100, 130, 365
MluCI AATT 5 cut(s) 26, 104, 345, 460, 593
MlyI GAGTC 1 cut(s) 557
MnlI CCTC 4 cut(s) 204, 210, 481, 550
MroXI GAANNNNTTC 1 cut(s) 539
MseI TTAA 1 cut(s) 30
MslI CAYNNNNRTG 2 cut(s) 354, 738
MspI CCGG 2 cut(s) 36, 112
MspR9I CCNGG 1 cut(s) 289
MvaI CCWGG 1 cut(s) 289
MwoI GCNNNNNNNGC 2 cut(s) 81, 167
NdeI CATATG 1 cut(s) 124
NdeII GATC 3 cut(s) 99, 358, 705
NlaIII CATG 3 cut(s) 436, 663, 716
NlaIV GGNNCC 3 cut(s) 326, 525, 701
NspI RCATGY 1 cut(s) 436
PaeR7I CTCGAG 1 cut(s) 208
PaqCI CACCTGC 1 cut(s) 616
PasI CCCWGGG 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 539
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 287
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 3 cut(s) 326, 525, 701
PspPI GGNCC 1 cut(s) 465
RsaI GTAC 4 cut(s) 93, 326, 429, 457
RsaNI GTAC 4 cut(s) 92, 325, 428, 456
RseI CAYNNNNRTG 2 cut(s) 354, 738
SalI GTCGAC 1 cut(s) 729
SaqAI TTAA 1 cut(s) 30
Sau3AI GATC 3 cut(s) 99, 358, 705
Sau96I GGNCC 1 cut(s) 465
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 289
SetI ASST 9 cut(s) 25, 53, 93, 263, 280, 326, 330, 522, 630
SfcI CTRYAG 1 cut(s) 508
Sfr274I CTCGAG 1 cut(s) 208
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 465
SlaI CTCGAG 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 354, 738
SmlI CTYRAG 2 cut(s) 194, 208
SmoI CTYRAG 2 cut(s) 194, 208
Sse9I AATT 5 cut(s) 26, 104, 345, 460, 593
SsiI CCGC 1 cut(s) 202
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 287
TaaI ACNGT 1 cut(s) 366
TaiI ACGT 2 cut(s) 25, 93
TaqI TCGA 5 cut(s) 209, 483, 546, 679, 730
TasI AATT 5 cut(s) 26, 104, 345, 460, 593
TatI WGTACW 1 cut(s) 427
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 1 cut(s) 701
TspDTI ATGAA 2 cut(s) 69, 532
TspRI CASTG 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 465
XapI RAATTY 3 cut(s) 26, 104, 345
XceI RCATGY 1 cut(s) 436
XhoI CTCGAG 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 730
XmnI GAANNNNTTC 1 cut(s) 539
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.