RchiOBHm_Chr3g0484461

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000672
Physical Location & Seq
Forward (+)
30814620 .. 30815620
1001 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44917

Sequence Viewer

Length: 822 bp
ATGGCCGCCGCTGACCAAGAATCCAGTGTTGAAGCAAGGATGAACAGCATCACCGAAACACGAAAGTTTGCAGTCCAAGTTCATCACAATAGAGCAAACTACTACCGCAGCAGTTCCGGCGTGGACATACTTCCGATCAGATTCAAATGGGGCAAGATAACTGTAAACTACAATCCAAGTATCCAAGGTTATGATTGTGAACCTCCAGATATTATATCCAGACGTACTCTTAAGCTGCGGTTCTCGAGGGAGAGGGCGAGTGGGAGTGCAAAGATAACGGAGAGGAGGTTGGCCAAGTACAGAAGGGTCATAGCTAGGTCTCCGACGGAAATGGACAAGTTACAGATGAAGCTGGCTAGGTTTCTGCGCGGCCCTGTGGGTATTCCCGAAGATAAGCACCCGTTTATTATTGTACATATATTGGATATGATTGAATTAGCCGACCCAGATAAAATTATCGATGTGCACTTGATGGACATGACTGTTAGAGCTACGGGGGAATTCGCGCACTTAAACGTGGAAGACAGTTACTACAGGCTCATGCTTATTCCGGCATCCGAAGCATTCATTCAGGGTTTGCAGCAAGTGAGACTCGTTGAGCATGACAGTTTGGAAATGCTTGGTCTGGAGACATCGTGCGCTGTTTGTCTGGATGCCTTTGCGGACACAAAAGAGATCACCATCGCTCGCTTGCCCTGCTTACATTATTATCATCATCATTGCATTGTCGAGTGGCTCAGGATCAATCACGTCTGCCCCATTTGCCGGCACCCTAATGCCCCGATTAGATGCCCGGGGTCATCAAGGCAGGGAAGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

273

Amino Acids

31.26

Weight (kDa)

8.6

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 212 - 256 3.1e-12 Ring finger domain
zf-C3HC4_2 PF13923 213 - 255 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 213 - 255 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 213 - 242 6.1e-06 RING-like zinc finger
zf-rbx1 PF12678 216 - 256 1.3e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 768
AccII CGCG 2 cut(s) 369, 506
AciI CCGC 6 cut(s) 6, 9, 106, 238, 369, 662
AclWI GGATC 1 cut(s) 749
AcoI YGGCCR 2 cut(s) 3, 291
AcsI RAATTY 1 cut(s) 500
AfaI GTAC 3 cut(s) 226, 299, 414
AfiI CCNNNNNNNGG 1 cut(s) 765
AflII CTTAAG 1 cut(s) 230
AgsI TTSAA 3 cut(s) 32, 145, 434
AjiI CACGTC 1 cut(s) 751
AluBI AGCT 4 cut(s) 235, 314, 352, 491
AluI AGCT 4 cut(s) 235, 314, 352, 491
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 3 cut(s) 324, 583, 623
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 1 cut(s) 749
Ama87I CYCGRG 2 cut(s) 244, 793
AoxI GGCC 3 cut(s) 3, 291, 370
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 108, 235, 580
ApoI RAATTY 1 cut(s) 500
AspLEI GCGC 3 cut(s) 369, 508, 641
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 794, 795
AsuHPI GGTGA 2 cut(s) 43, 670
AvaI CYCGRG 2 cut(s) 244, 793
BaeGI GKGCMC 1 cut(s) 468
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 1 cut(s) 768
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 3 cut(s) 120, 222, 592
BccI CCATC 2 cut(s) 466, 689
BciVI GTATCC 1 cut(s) 191
BcnI CCSGG 2 cut(s) 794, 795
BcoDI GTCTC 3 cut(s) 324, 583, 623
BfaI CTAG 2 cut(s) 315, 357
BfmI CTRYAG 1 cut(s) 532
BfrI CTTAAG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 191
BisI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 581
BlsI GCNGC 6 cut(s) 7, 10, 110, 237, 371, 582
Bme1390I CCNGG 2 cut(s) 794, 795
BmeT110I CYCGRG 2 cut(s) 244, 793
BmgBI CACGTC 1 cut(s) 751
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 770
BmrFI CCNGG 2 cut(s) 794, 795
BmsI GCATC 4 cut(s) 57, 563, 643, 779
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 2 cut(s) 189, 647
Bpu10I CCTNAGC 1 cut(s) 737
BpuMI CCSGG 2 cut(s) 794, 795
Bsa29I ATCGAT 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 680
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 184, 793, 794
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 620, 650
Bsc4I CCNNNNNNNGG 1 cut(s) 765
Bse118I RCCGGY 1 cut(s) 765
Bse1I ACTGG 2 cut(s) 24, 817
Bse3DI GCAATG 1 cut(s) 718
Bse8I GATNNNNATC 1 cut(s) 680
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 184, 793, 794
BseGI GGATG 3 cut(s) 45, 554, 658
BseJI GATNNNNATC 1 cut(s) 680
BseLI CCNNNNNNNGG 1 cut(s) 765
BseMI GCAATG 1 cut(s) 718
BseMII CTCAG 1 cut(s) 751
BseNI ACTGG 2 cut(s) 24, 817
BseRI GAGGAG 1 cut(s) 298
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 3 cut(s) 120, 222, 592
Bsh1236I CGCG 2 cut(s) 369, 506
BshFI GGCC 3 cut(s) 5, 293, 372
BshNI GGYRCC 1 cut(s) 768
BshVI ATCGAT 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 2 cut(s) 244, 793
BsiSI CCGG 4 cut(s) 117, 551, 766, 794
BslI CCNNNNNNNGG 1 cut(s) 765
BsmAI GTCTC 3 cut(s) 324, 583, 623
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 3 cut(s) 5, 293, 372
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 2 cut(s) 244, 793
Bsp1286I GDGCHC 1 cut(s) 468
Bsp1407I TGTACA 1 cut(s) 412
Bsp143I GATC 3 cut(s) 135, 675, 741
BspACI CCGC 6 cut(s) 6, 9, 106, 238, 369, 662
BspANI GGCC 3 cut(s) 5, 293, 372
BspCNI CTCAG 1 cut(s) 750
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 2 cut(s) 369, 506
BspLI GGNNCC 1 cut(s) 770
BspPI GGATC 1 cut(s) 749
BspT107I GGYRCC 1 cut(s) 768
BspTI CTTAAG 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 718
BsrFI RCCGGY 1 cut(s) 765
BsrGI TGTACA 1 cut(s) 412
BsrI ACTGG 2 cut(s) 24, 817
BssAI RCCGGY 1 cut(s) 765
BssECI CCNNGG 3 cut(s) 184, 793, 794
BssMI GATC 3 cut(s) 135, 675, 741
BssT1I CCWWGG 1 cut(s) 184
Bst4CI ACNGT 4 cut(s) 163, 484, 527, 608
BstAFI CTTAAG 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 412
BstC8I GCNNGC 4 cut(s) 354, 688, 692, 767
BstDEI CTNAG 1 cut(s) 737
BstF5I GGATG 3 cut(s) 45, 554, 658
BstFNI CGCG 2 cut(s) 369, 506
BstHHI GCGC 3 cut(s) 369, 508, 641
BstKTI GATC 3 cut(s) 138, 678, 744
BstMAI GTCTC 3 cut(s) 324, 583, 623
BstMBI GATC 3 cut(s) 135, 675, 741
BstMWI GCNNNNNNNGC 4 cut(s) 117, 560, 696, 762
BstSCI CCNGG 2 cut(s) 792, 793
BstSFI CTRYAG 1 cut(s) 532
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 2 cut(s) 369, 506
BstV1I GCAGC 3 cut(s) 120, 222, 592
BstV2I GAAGAC 1 cut(s) 528
Bsu15I ATCGAT 1 cut(s) 459
BsuI GTATCC 1 cut(s) 191
BsuRI GGCC 3 cut(s) 5, 293, 372
BsuTUI ATCGAT 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 667
BtrI CACGTC 1 cut(s) 751
BtsCI GGATG 3 cut(s) 45, 554, 658
BtsIMutI CAGTG 1 cut(s) 31
Cac8I GCNNGC 4 cut(s) 354, 688, 692, 767
CfoI GCGC 3 cut(s) 369, 508, 641
Cfr10I RCCGGY 1 cut(s) 765
Cfr13I GGNCC 1 cut(s) 371
Cfr9I CCCGGG 1 cut(s) 793
ClaI ATCGAT 1 cut(s) 459
Csp6I GTAC 3 cut(s) 225, 298, 413
CviAII CATG 3 cut(s) 478, 541, 602
CviQI GTAC 3 cut(s) 225, 298, 413
DdeI CTNAG 1 cut(s) 737
DpnI GATC 3 cut(s) 137, 677, 743
DpnII GATC 3 cut(s) 135, 675, 741
EaeI YGGCCR 2 cut(s) 3, 291
Eco130I CCWWGG 1 cut(s) 184
Eco31I GGTCTC 1 cut(s) 324
Eco88I CYCGRG 2 cut(s) 244, 793
EcoRI GAATTC 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 3 cut(s) 481, 544, 605
FatI CATG 3 cut(s) 477, 540, 601
Fnu4HI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 581
FokI GGATG 3 cut(s) 52, 541, 665
Fsp4HI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 581
FspBI CTAG 2 cut(s) 315, 357
GlaI GCGC 3 cut(s) 368, 507, 640
GluI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 581
GsuI CTGGAG 2 cut(s) 189, 647
HaeIII GGCC 3 cut(s) 5, 293, 372
HapII CCGG 4 cut(s) 117, 551, 766, 794
HhaI GCGC 3 cut(s) 369, 508, 641
Hin1II CATG 3 cut(s) 481, 544, 605
Hin6I GCGC 3 cut(s) 367, 506, 639
HinP1I GCGC 3 cut(s) 367, 506, 639
HinfI GANTC 3 cut(s) 20, 141, 591
HpaII CCGG 4 cut(s) 117, 551, 766, 794
HphI GGTGA 2 cut(s) 43, 670
Hpy166II GTNNAC 4 cut(s) 124, 166, 200, 466
Hpy188I TCNGA 4 cut(s) 135, 140, 324, 559
Hpy188III TCNNGA 7 cut(s) 206, 219, 244, 386, 626, 650, 739
Hpy8I GTNNAC 4 cut(s) 124, 166, 200, 466
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 297
HpyCH4III ACNGT 4 cut(s) 163, 484, 527, 608
HpyCH4IV ACGT 3 cut(s) 223, 516, 750
HpyCH4V TGCA 5 cut(s) 71, 269, 466, 580, 723
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 560, 696, 762
HpyF3I CTNAG 1 cut(s) 737
HpySE526I ACGT 3 cut(s) 223, 516, 750
Hsp92II CATG 3 cut(s) 481, 544, 605
HspAI GCGC 3 cut(s) 367, 506, 639
KroI GCCGGC 1 cut(s) 765
KroNI GCCGGC 1 cut(s) 767
Kzo9I GATC 3 cut(s) 135, 675, 741
Lsp1109I GCAGC 3 cut(s) 120, 222, 592
LweI GCATC 4 cut(s) 57, 563, 643, 779
MaeI CTAG 2 cut(s) 315, 357
MaeII ACGT 3 cut(s) 223, 516, 750
MaeIII GTNAC 2 cut(s) 339, 527
MalI GATC 3 cut(s) 137, 677, 743
MboI GATC 3 cut(s) 135, 675, 741
MboII GAAGA 2 cut(s) 401, 533
MhlI GDGCHC 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 3 cut(s) 434, 453, 500
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 1 cut(s) 585
MmeI TCCRAC 1 cut(s) 347
MnlI CCTC 5 cut(s) 213, 240, 246, 276, 279
Mox20I TGGCCA 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 765
MscI TGGCCA 1 cut(s) 293
MseI TTAA 2 cut(s) 231, 512
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 11
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 4 cut(s) 117, 551, 766, 794
MspR9I CCNGG 2 cut(s) 794, 795
Mva1269I GAATGC 1 cut(s) 563
MvnI CGCG 2 cut(s) 369, 506
MwoI GCNNNNNNNGC 4 cut(s) 117, 560, 696, 762
NaeI GCCGGC 1 cut(s) 767
NciI CCSGG 2 cut(s) 794, 795
NdeII GATC 3 cut(s) 135, 675, 741
NgoMIV GCCGGC 1 cut(s) 765
NlaIII CATG 3 cut(s) 481, 544, 605
NlaIV GGNNCC 1 cut(s) 770
PaeR7I CTCGAG 1 cut(s) 244
PctI GAATGC 1 cut(s) 563
PdiI GCCGGC 1 cut(s) 767
PfeI GAWTC 2 cut(s) 20, 141
PkrI GCNGC 6 cut(s) 7, 10, 110, 237, 371, 582
PleI GAGTC 1 cut(s) 585
PpsI GAGTC 1 cut(s) 585
PspN4I GGNNCC 1 cut(s) 770
PspPI GGNCC 1 cut(s) 371
RsaI GTAC 3 cut(s) 226, 299, 414
RsaNI GTAC 3 cut(s) 225, 298, 413
RseI CAYNNNNRTG 1 cut(s) 774
SaqAI TTAA 2 cut(s) 231, 512
SatI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 581
Sau3AI GATC 3 cut(s) 135, 675, 741
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 1 cut(s) 585
ScrFI CCNGG 2 cut(s) 794, 795
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 4 cut(s) 57, 563, 643, 779
SfcI CTRYAG 1 cut(s) 532
Sfr274I CTCGAG 1 cut(s) 244
SlaI CTCGAG 1 cut(s) 244
SmaI CCCGGG 1 cut(s) 795
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 2 cut(s) 230, 244
SmoI CTYRAG 2 cut(s) 230, 244
Sse9I AATT 3 cut(s) 434, 453, 500
SsiI CCGC 6 cut(s) 6, 9, 106, 238, 369, 662
SspMI CTAG 2 cut(s) 315, 357
StyD4I CCNGG 2 cut(s) 792, 793
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 4 cut(s) 163, 484, 527, 608
TaiI ACGT 3 cut(s) 226, 519, 753
TaqI TCGA 3 cut(s) 245, 459, 729
TasI AATT 3 cut(s) 434, 453, 500
TatI WGTACW 2 cut(s) 297, 412
TauI GCSGC 3 cut(s) 8, 11, 372
TfiI GAWTC 2 cut(s) 20, 141
Tru1I TTAA 2 cut(s) 231, 512
Tru9I TTAA 2 cut(s) 231, 512
TscAI CASTG 1 cut(s) 31
TseI GCWGC 3 cut(s) 108, 235, 580
TspDTI ATGAA 4 cut(s) 56, 71, 362, 556
TspGWI ACGGA 2 cut(s) 293, 341
TspMI CCCGGG 1 cut(s) 793
TspRI CASTG 1 cut(s) 31
Vha464I CTTAAG 1 cut(s) 230
VneI GTGCAC 1 cut(s) 464
XapI RAATTY 1 cut(s) 500
XhoI CTCGAG 1 cut(s) 244
XmaI CCCGGG 1 cut(s) 793
XspI CTAG 2 cut(s) 315, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.