Rw6G005950

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000672
Physical Location & Seq
Reverse (-)
9539482 .. 9548892
9411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G005950.1

Sequence Viewer

Length: 1077 bp
ATGGATGCCAAAATCAACTGGGGTTTAGAGCAGGCCTTCAATTTGAAGCTTGATTGCAGTGAGATTGGGGATGACAGGCCCCTCTCTGGTTGTTCGCCGCAACCTCTAATCGCACTTCGCTCCGGTGCTTCGCTGCTACTTCCCGTGGCTTCGAGAAAGTCCCGGTTCAGAATCCGATCGTCGAAATGGACGGTGGAGAGATTCCTCCTGAGATCGAAAAGATGGGCAGCTTGGAAGAGACAGAGAGAAGAAAGCAGAGGGAGCTGGGTAGAAGAAGAAGAAGAAGAAGAAGAGATTACGAATTGCATTTTCAGAGCTCTTTTGCCCGTTCTCCTATCAGCTTCCAGCTCCCGCAACGATGATAGCCCCAATACTAAGCATTGGGAGAAAGCCTTTGACGGTCTTGCTCCAAGCGAACACCGTTTGCTGCAGCCAAATCAAGAAGCTTCTGGTGCTGCTTCTATGGCTTGGATGATGGCTCACCCGCTTGAGGAGTTCTTTGAAGCAGATAGGAAGACTGATGACGAAATGCCATGGTTGTATATGAACACAGGCAAAACAATTGAGAGGAAGATGGTTAGGTATCTTTCAAGGATTGGAGCTCCGGAAGAAGAGCACGAGTCTATTATTGAAAAAATATTGGAGGAGCTTAAAGATGCTGTCCCCGACTTGATACTTGATGTGCACATTGCGAAATTCATAACTAGATACACGGCCGAGCATTTCTTCGATGACACACACTACAGGGGCAAGCTAATTCCTGCAACCGAATCATTTATCCAGGGTTTGGAGAAAGTGAGATCACTAGAGAGTTTTGGTGGACATACCTGTGCTATTTGTATGGAGGCATTTGCTGCACATAATCATCTGAATCGGTTGCCCTGCTTGCACTCTTATCATCAACATTGCATTGTCCAGTGGCTCCAAATCAATCACGTCTGCCCCATTTGTAGACACCTTTATGCTACTGCCTCTGCCGAGCCATCAGACCCTAATCCCAAATTCCCAATTGATATTGTTTGCTGCAAACTAGCTATTGACATTAGTATTTCCCAATTGATATATGCCGGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

358

Amino Acids

40.97

Weight (kDa)

5.86

Isoelectric Point (pI)

61.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 276 - 318 9.8e-12 Ring finger domain
zf-C3HC4_2 PF13923 277 - 317 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 277 - 317 6.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 277 - 304 8.9e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 787
AccI GTMKAC 1 cut(s) 952
AccIII TCCGGA 1 cut(s) 604
AciI CCGC 3 cut(s) 98, 352, 485
AcoI YGGCCR 1 cut(s) 714
AcsI RAATTY 2 cut(s) 695, 1001
AfiI CCNNNNNNNGG 3 cut(s) 86, 490, 787
AgsI TTSAA 5 cut(s) 40, 46, 503, 591, 632
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 1 cut(s) 780
Alw21I GWGCWC 4 cut(s) 319, 604, 618, 687
Alw26I GTCTC 1 cut(s) 232
Alw44I GTGCAC 1 cut(s) 683
Aor13HI TCCGGA 1 cut(s) 604
AoxI GGCC 3 cut(s) 33, 77, 714
ApaLI GTGCAC 1 cut(s) 683
ApeKI GCWGC 7 cut(s) 133, 227, 427, 430, 455, 854, 1023
ApoI RAATTY 2 cut(s) 695, 1001
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 687
BanII GRGCYC 2 cut(s) 319, 604
BauI CACGAG 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 521
Bbv12I GWGCWC 4 cut(s) 319, 604, 618, 687
BbvI GCAGC 7 cut(s) 120, 239, 414, 442, 442, 841, 1010
BccI CCATC 4 cut(s) 216, 469, 568, 991
BceAI ACGGC 1 cut(s) 729
BciT130I CCWGG 1 cut(s) 782
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 4 cut(s) 705, 806, 1031, 1075
BfmI CTRYAG 2 cut(s) 428, 742
BisI GCNGC 8 cut(s) 98, 134, 228, 428, 431, 456, 855, 1024
BlsI GCNGC 8 cut(s) 99, 135, 229, 429, 432, 457, 856, 1025
Bme1390I CCNGG 2 cut(s) 163, 782
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 2 cut(s) 80, 923
BmrFI CCNGG 2 cut(s) 163, 782
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 646
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 521
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 163
BsaJI CCNNGG 3 cut(s) 144, 533, 781
BsaWI WCCGGW 2 cut(s) 122, 604
Bsc4I CCNNNNNNNGG 3 cut(s) 86, 490, 787
Bse1I ACTGG 2 cut(s) 23, 916
Bse3DI GCAATG 2 cut(s) 687, 904
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 1 cut(s) 782
BseDI CCNNGG 3 cut(s) 144, 533, 781
BseGI GGATG 3 cut(s) 10, 76, 477
BseLI CCNNNNNNNGG 3 cut(s) 86, 490, 787
BseMI GCAATG 2 cut(s) 687, 904
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 23, 916
BseRI GAGGAG 2 cut(s) 506, 659
BseSI GKGCMC 1 cut(s) 687
BseX3I CGGCCG 1 cut(s) 714
BseXI GCAGC 7 cut(s) 120, 239, 414, 442, 442, 841, 1010
BseYI CCCAGC 1 cut(s) 264
BsgI GTGCAG 1 cut(s) 840
Bsh1285I CGRYCG 2 cut(s) 179, 717
BshFI GGCC 3 cut(s) 35, 79, 716
BsiEI CGRYCG 2 cut(s) 179, 717
BsiHKAI GWGCWC 4 cut(s) 319, 604, 618, 687
BsiSI CCGG 4 cut(s) 123, 163, 605, 1068
BslFI GGGAC 2 cut(s) 145, 647
BslI CCNNNNNNNGG 3 cut(s) 86, 490, 787
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 2 cut(s) 145, 647
BsnI GGCC 3 cut(s) 35, 79, 716
Bsp1286I GDGCHC 4 cut(s) 319, 604, 618, 687
Bsp13I TCCGGA 1 cut(s) 604
Bsp143I GATC 3 cut(s) 176, 212, 800
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 3 cut(s) 98, 352, 485
BspANI GGCC 3 cut(s) 35, 79, 716
BspCNI CTCAG 1 cut(s) 201
BspEI TCCGGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 80, 923
BspMAI CTGCAG 1 cut(s) 432
BspQI GCTCTTC 1 cut(s) 606
BsrDI GCAATG 2 cut(s) 687, 904
BsrI ACTGG 2 cut(s) 23, 916
BssECI CCNNGG 3 cut(s) 144, 533, 781
BssMI GATC 3 cut(s) 176, 212, 800
BssSI CACGAG 1 cut(s) 617
BssT1I CCWWGG 1 cut(s) 533
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 1 cut(s) 782
Bst4CI ACNGT 3 cut(s) 193, 401, 422
Bst6I CTCTTC 3 cut(s) 230, 285, 606
BstAPI GCANNNNNTGC 1 cut(s) 854
BstC8I GCNNGC 3 cut(s) 33, 752, 887
BstDEI CTNAG 2 cut(s) 209, 375
BstDSI CCRYGG 2 cut(s) 144, 533
BstF5I GGATG 3 cut(s) 10, 76, 477
BstKTI GATC 3 cut(s) 179, 215, 803
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 3 cut(s) 176, 212, 800
BstMCI CGRYCG 2 cut(s) 179, 717
BstMWI GCNNNNNNNGC 5 cut(s) 261, 452, 464, 854, 886
BstNI CCWGG 1 cut(s) 782
BstSCI CCNGG 2 cut(s) 161, 780
BstSFI CTRYAG 2 cut(s) 428, 742
BstSLI GKGCMC 1 cut(s) 687
BstV1I GCAGC 7 cut(s) 120, 239, 414, 442, 442, 841, 1010
BstV2I GAAGAC 1 cut(s) 521
BstZI CGGCCG 1 cut(s) 714
BsuRI GGCC 3 cut(s) 35, 79, 716
BtgI CCRYGG 2 cut(s) 144, 533
BtrI CACGTC 1 cut(s) 937
BtsCI GGATG 3 cut(s) 10, 76, 477
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 2 cut(s) 64, 923
Cac8I GCNNGC 3 cut(s) 33, 752, 887
Cfr13I GGNCC 1 cut(s) 78
CviAII CATG 1 cut(s) 534
DdeI CTNAG 2 cut(s) 209, 375
DpnI GATC 3 cut(s) 178, 214, 802
DpnII GATC 3 cut(s) 176, 212, 800
EaeI YGGCCR 1 cut(s) 714
EagI CGGCCG 1 cut(s) 714
Eam1104I CTCTTC 3 cut(s) 230, 285, 606
EarI CTCTTC 3 cut(s) 230, 285, 606
Ecl136II GAGCTC 2 cut(s) 317, 602
EclXI CGGCCG 1 cut(s) 714
Eco130I CCWWGG 1 cut(s) 533
Eco147I AGGCCT 1 cut(s) 35
Eco24I GRGCYC 2 cut(s) 319, 604
Eco52I CGGCCG 1 cut(s) 714
Eco53kI GAGCTC 2 cut(s) 317, 602
EcoICRI GAGCTC 2 cut(s) 317, 602
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 533
EcoT38I GRGCYC 2 cut(s) 319, 604
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 1 cut(s) 537
FaqI GGGAC 2 cut(s) 145, 647
FatI CATG 1 cut(s) 533
FauI CCCGC 2 cut(s) 359, 492
FblI GTMKAC 1 cut(s) 952
Fnu4HI GCNGC 8 cut(s) 98, 134, 228, 428, 431, 456, 855, 1024
FokI GGATG 3 cut(s) 17, 83, 484
FriOI GRGCYC 2 cut(s) 319, 604
Fsp4HI GCNGC 8 cut(s) 98, 134, 228, 428, 431, 456, 855, 1024
FspBI CTAG 4 cut(s) 705, 806, 1031, 1075
GluI GCNGC 8 cut(s) 98, 134, 228, 428, 431, 456, 855, 1024
GsaI CCCAGC 1 cut(s) 268
HaeIII GGCC 3 cut(s) 35, 79, 716
HapII CCGG 4 cut(s) 123, 163, 605, 1068
Hin1II CATG 1 cut(s) 537
HindIII AAGCTT 2 cut(s) 47, 444
HinfI GANTC 5 cut(s) 171, 201, 620, 770, 871
HpaII CCGG 4 cut(s) 123, 163, 605, 1068
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 3 cut(s) 685, 821, 953
Hpy188I TCNGA 5 cut(s) 170, 176, 314, 870, 988
Hpy188III TCNNGA 4 cut(s) 153, 208, 440, 605
Hpy8I GTNNAC 3 cut(s) 685, 821, 953
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 3 cut(s) 193, 401, 422
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 9 cut(s) 57, 306, 430, 685, 764, 857, 889, 909, 1026
HpyF10VI GCNNNNNNNGC 5 cut(s) 261, 452, 464, 854, 886
HpyF3I CTNAG 2 cut(s) 209, 375
HpySE526I ACGT 1 cut(s) 936
Hsp92II CATG 1 cut(s) 537
Kpn2I TCCGGA 1 cut(s) 604
Kzo9I GATC 3 cut(s) 176, 212, 800
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 8 cut(s) 125, 261, 353, 412, 599, 607, 646, 927
Lsp1109I GCAGC 7 cut(s) 120, 239, 414, 442, 442, 841, 1010
LweI GCATC 1 cut(s) 646
MaeI CTAG 4 cut(s) 705, 806, 1031, 1075
MaeII ACGT 1 cut(s) 936
MalI GATC 3 cut(s) 178, 214, 802
MboI GATC 3 cut(s) 176, 212, 800
MfeI CAATTG 3 cut(s) 561, 1008, 1055
MhlI GDGCHC 4 cut(s) 319, 604, 618, 687
MluCI AATT 8 cut(s) 40, 301, 561, 695, 756, 1001, 1008, 1055
MlyI GAGTC 1 cut(s) 629
MnlI CCTC 9 cut(s) 92, 114, 215, 251, 484, 561, 637, 838, 982
MroI TCCGGA 1 cut(s) 604
MseI TTAA 1 cut(s) 651
MslI CAYNNNNRTG 2 cut(s) 828, 960
MspI CCGG 4 cut(s) 123, 163, 605, 1068
MspR9I CCNGG 2 cut(s) 163, 782
MunI CAATTG 3 cut(s) 561, 1008, 1055
MvaI CCWGG 1 cut(s) 782
MwoI GCNNNNNNNGC 5 cut(s) 261, 452, 464, 854, 886
NciI CCSGG 1 cut(s) 163
NcoI CCATGG 1 cut(s) 533
NdeII GATC 3 cut(s) 176, 212, 800
NlaIII CATG 1 cut(s) 537
NlaIV GGNNCC 2 cut(s) 80, 923
NmeAIII GCCGAG 2 cut(s) 742, 1003
PceI AGGCCT 1 cut(s) 35
PciSI GCTCTTC 1 cut(s) 606
PfeI GAWTC 4 cut(s) 171, 201, 770, 871
PflMI CCANNNNNTGG 1 cut(s) 787
PkrI GCNGC 8 cut(s) 99, 135, 229, 429, 432, 457, 856, 1025
Ple19I CGATCG 1 cut(s) 179
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
Psp124BI GAGCTC 2 cut(s) 319, 604
Psp6I CCWGG 1 cut(s) 780
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 1 cut(s) 780
PspN4I GGNNCC 2 cut(s) 80, 923
PspPI GGNCC 1 cut(s) 78
PstI CTGCAG 1 cut(s) 432
PvuI CGATCG 1 cut(s) 179
RseI CAYNNNNRTG 2 cut(s) 828, 960
SacI GAGCTC 2 cut(s) 319, 604
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 8 cut(s) 98, 134, 228, 428, 431, 456, 855, 1024
Sau3AI GATC 3 cut(s) 176, 212, 800
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 2 cut(s) 163, 782
SduI GDGCHC 4 cut(s) 319, 604, 618, 687
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 2 cut(s) 428, 742
SmiMI CAYNNNNRTG 2 cut(s) 828, 960
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 8 cut(s) 40, 301, 561, 695, 756, 1001, 1008, 1055
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 3 cut(s) 98, 352, 485
SspI AATATT 1 cut(s) 639
SspMI CTAG 4 cut(s) 705, 806, 1031, 1075
SstI GAGCTC 2 cut(s) 319, 604
StuI AGGCCT 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 161, 780
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 3 cut(s) 193, 401, 422
TaiI ACGT 1 cut(s) 939
TaqI TCGA 4 cut(s) 152, 182, 215, 729
TasI AATT 8 cut(s) 40, 301, 561, 695, 756, 1001, 1008, 1055
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 4 cut(s) 171, 201, 770, 871
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 2 cut(s) 64, 923
TseI GCWGC 7 cut(s) 133, 227, 427, 430, 455, 854, 1023
TspDTI ATGAA 2 cut(s) 560, 688
TspRI CASTG 2 cut(s) 64, 923
Van91I CCANNNNNTGG 1 cut(s) 787
VneI GTGCAC 1 cut(s) 683
XapI RAATTY 2 cut(s) 695, 1001
XmiI GTMKAC 1 cut(s) 952
XspI CTAG 4 cut(s) 705, 806, 1031, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.