Rh3AG260200

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000672
Physical Location & Seq
Forward (+)
28923756 .. 28944335
20580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG260200.1

Sequence Viewer

Length: 876 bp
ATGCCGACCAATCCCTTGTGGCCAACTCATCACAATTCCTATATAAATCACCTTATGGCCGCCGCTGACCAAGAATCCAGTGTTGAAGCAAGGATGAACAGCATCACCGAAACACGACAGTTTGCAGTCCAAGTTCATCACAATAGAGCAAACTACTACCGCAGCAGTTCCGGCGTGGACATACTTCCGATCAGATTCAAATGGGCCAAGGTAACTGTAAACTACAATCCAAGTATCCAAGGTTATGATTGTGAACCTCCAGATATTTTATCCAGACGTACTCTTAAGCTGCGGTTCTCGAGGGAGAGGGCGAGTGGGAGTGCAAAGATAACGGAGAGGAGGTTGGCCAAGTACAGAAGGGTCATAGCTAGGTCTCCGACGGAAATGGACAAGTTACAGATGAAGCTGGCTAGGTTTCTGCGCAGCCCTGTGGGTATTCCCGAAGATAAGCACCCGTTTATTATTGTACATATATTGGATATGATTGAATTAGCCGACCCAGATAAAATTATCGATGTACACTTGATGGACGTAACTGTTAGAGCTACGGGGGAATTCGCGCACTTAAACGTGGAAGACAATTACTACAGGCTCACGCTTATTCCGGCATCCGAAGCATTCATTCAAGGTTTGCAGCAAGTGAGACTCGTTGAGCATGACAGTTTGGAAATGCTTGGTCTGGAGACATCGTGCGCTGTTTGTCTGGATGCCTTTGCGGACACAAAAGAGATCACCATCGCTCGCTTGCCCTGCTTACATTATTATCATCATCATTGCATTGTCGAGTGGCTCAGGATCAATCACGTCTGCCCCATTTGCCGGCACCCTAATGCCCCGATTAGATGCCCGGGGTCATCAAGGCAGGGAAGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

291

Amino Acids

33.41

Weight (kDa)

8.4

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 230 - 274 3.4e-12 Ring finger domain
zf-C3HC4_2 PF13923 231 - 273 3.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 231 - 273 2.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 231 - 260 6.7e-06 RING-like zinc finger
zf-rbx1 PF12678 234 - 274 1.4e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 822
AccII CGCG 1 cut(s) 560
AciI CCGC 5 cut(s) 60, 63, 160, 292, 716
AclWI GGATC 1 cut(s) 803
AcoI YGGCCR 3 cut(s) 20, 57, 345
AcsI RAATTY 1 cut(s) 554
AfaI GTAC 4 cut(s) 280, 353, 468, 519
AfiI CCNNNNNNNGG 1 cut(s) 819
AflII CTTAAG 1 cut(s) 284
AgsI TTSAA 4 cut(s) 86, 199, 488, 626
AjiI CACGTC 1 cut(s) 805
AluBI AGCT 4 cut(s) 289, 368, 406, 545
AluI AGCT 4 cut(s) 289, 368, 406, 545
Alw26I GTCTC 3 cut(s) 378, 637, 677
AlwI GGATC 1 cut(s) 803
Ama87I CYCGRG 2 cut(s) 298, 847
AoxI GGCC 4 cut(s) 20, 57, 204, 345
ApeKI GCWGC 4 cut(s) 162, 289, 423, 634
ApoI RAATTY 1 cut(s) 554
AspLEI GCGC 3 cut(s) 423, 562, 695
AspS9I GGNCC 1 cut(s) 204
AsuC2I CCSGG 2 cut(s) 848, 849
AsuHPI GGTGA 3 cut(s) 41, 97, 724
AvaI CYCGRG 2 cut(s) 298, 847
BalI TGGCCA 2 cut(s) 22, 347
BanI GGYRCC 1 cut(s) 822
BbsI GAAGAC 1 cut(s) 582
BbvI GCAGC 4 cut(s) 174, 276, 435, 646
BccI CCATC 2 cut(s) 520, 743
BciVI GTATCC 1 cut(s) 245
BcnI CCSGG 2 cut(s) 848, 849
BcoDI GTCTC 3 cut(s) 378, 637, 677
BfaI CTAG 2 cut(s) 369, 411
BfmI CTRYAG 1 cut(s) 586
BfrI CTTAAG 1 cut(s) 284
BfuI GTATCC 1 cut(s) 245
BisI GCNGC 6 cut(s) 60, 63, 163, 290, 424, 635
BlsI GCNGC 6 cut(s) 61, 64, 164, 291, 425, 636
Bme1390I CCNGG 2 cut(s) 848, 849
BmeT110I CYCGRG 2 cut(s) 298, 847
BmgBI CACGTC 1 cut(s) 805
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 1 cut(s) 824
BmrFI CCNGG 2 cut(s) 848, 849
BmsI GCATC 4 cut(s) 111, 617, 697, 833
BpiI GAAGAC 1 cut(s) 582
BpmI CTGGAG 2 cut(s) 243, 701
Bpu10I CCTNAGC 1 cut(s) 791
BpuMI CCSGG 2 cut(s) 848, 849
Bsa29I ATCGAT 1 cut(s) 513
BsaBI GATNNNNATC 1 cut(s) 734
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 4 cut(s) 207, 238, 847, 848
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 674, 704
Bsc4I CCNNNNNNNGG 1 cut(s) 819
Bse118I RCCGGY 1 cut(s) 819
Bse1I ACTGG 2 cut(s) 78, 871
Bse3DI GCAATG 1 cut(s) 772
Bse8I GATNNNNATC 1 cut(s) 734
BseCI ATCGAT 1 cut(s) 513
BseDI CCNNGG 4 cut(s) 207, 238, 847, 848
BseGI GGATG 3 cut(s) 99, 608, 712
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 1 cut(s) 819
BseMI GCAATG 1 cut(s) 772
BseMII CTCAG 1 cut(s) 805
BseNI ACTGG 2 cut(s) 78, 871
BseRI GAGGAG 1 cut(s) 352
BseXI GCAGC 4 cut(s) 174, 276, 435, 646
Bsh1236I CGCG 1 cut(s) 560
BshFI GGCC 4 cut(s) 22, 59, 206, 347
BshNI GGYRCC 1 cut(s) 822
BshVI ATCGAT 1 cut(s) 513
BsiHKCI CYCGRG 2 cut(s) 298, 847
BsiSI CCGG 4 cut(s) 171, 605, 820, 848
BslI CCNNNNNNNGG 1 cut(s) 819
BsmAI GTCTC 3 cut(s) 378, 637, 677
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 4 cut(s) 22, 59, 206, 347
Bso31I GGTCTC 1 cut(s) 378
BsoBI CYCGRG 2 cut(s) 298, 847
Bsp1407I TGTACA 2 cut(s) 466, 517
Bsp143I GATC 3 cut(s) 189, 729, 795
BspACI CCGC 5 cut(s) 60, 63, 160, 292, 716
BspANI GGCC 4 cut(s) 22, 59, 206, 347
BspCNI CTCAG 1 cut(s) 804
BspDI ATCGAT 1 cut(s) 513
BspFNI CGCG 1 cut(s) 560
BspLI GGNNCC 1 cut(s) 824
BspPI GGATC 1 cut(s) 803
BspT107I GGYRCC 1 cut(s) 822
BspTI CTTAAG 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 772
BsrFI RCCGGY 1 cut(s) 819
BsrGI TGTACA 2 cut(s) 466, 517
BsrI ACTGG 2 cut(s) 78, 871
BssAI RCCGGY 1 cut(s) 819
BssECI CCNNGG 4 cut(s) 207, 238, 847, 848
BssMI GATC 3 cut(s) 189, 729, 795
BssT1I CCWWGG 2 cut(s) 207, 238
Bst4CI ACNGT 4 cut(s) 120, 217, 538, 662
BstAFI CTTAAG 1 cut(s) 284
BstAUI TGTACA 2 cut(s) 466, 517
BstC8I GCNNGC 4 cut(s) 408, 742, 746, 821
BstDEI CTNAG 1 cut(s) 791
BstF5I GGATG 3 cut(s) 99, 608, 712
BstFNI CGCG 1 cut(s) 560
BstHHI GCGC 3 cut(s) 423, 562, 695
BstKTI GATC 3 cut(s) 192, 732, 798
BstMAI GTCTC 3 cut(s) 378, 637, 677
BstMBI GATC 3 cut(s) 189, 729, 795
BstMWI GCNNNNNNNGC 4 cut(s) 171, 614, 750, 816
BstSCI CCNGG 2 cut(s) 846, 847
BstSFI CTRYAG 1 cut(s) 586
BstUI CGCG 1 cut(s) 560
BstV1I GCAGC 4 cut(s) 174, 276, 435, 646
BstV2I GAAGAC 1 cut(s) 582
Bsu15I ATCGAT 1 cut(s) 513
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 4 cut(s) 22, 59, 206, 347
BsuTUI ATCGAT 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 721
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 3 cut(s) 99, 608, 712
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 4 cut(s) 408, 742, 746, 821
CfoI GCGC 3 cut(s) 423, 562, 695
Cfr10I RCCGGY 1 cut(s) 819
Cfr13I GGNCC 1 cut(s) 204
Cfr9I CCCGGG 1 cut(s) 847
ClaI ATCGAT 1 cut(s) 513
Csp6I GTAC 4 cut(s) 279, 352, 467, 518
CviAII CATG 1 cut(s) 656
CviQI GTAC 4 cut(s) 279, 352, 467, 518
DdeI CTNAG 1 cut(s) 791
DpnI GATC 3 cut(s) 191, 731, 797
DpnII GATC 3 cut(s) 189, 729, 795
EaeI YGGCCR 3 cut(s) 20, 57, 345
Eco130I CCWWGG 2 cut(s) 207, 238
Eco31I GGTCTC 1 cut(s) 378
Eco88I CYCGRG 2 cut(s) 298, 847
EcoRI GAATTC 1 cut(s) 554
EcoT14I CCWWGG 2 cut(s) 207, 238
ErhI CCWWGG 2 cut(s) 207, 238
FaeI CATG 1 cut(s) 659
FatI CATG 1 cut(s) 655
Fnu4HI GCNGC 6 cut(s) 60, 63, 163, 290, 424, 635
FokI GGATG 3 cut(s) 106, 595, 719
Fsp4HI GCNGC 6 cut(s) 60, 63, 163, 290, 424, 635
FspBI CTAG 2 cut(s) 369, 411
FspI TGCGCA 1 cut(s) 422
GlaI GCGC 3 cut(s) 422, 561, 694
GluI GCNGC 6 cut(s) 60, 63, 163, 290, 424, 635
GsuI CTGGAG 2 cut(s) 243, 701
HaeIII GGCC 4 cut(s) 22, 59, 206, 347
HapII CCGG 4 cut(s) 171, 605, 820, 848
HhaI GCGC 3 cut(s) 423, 562, 695
Hin1II CATG 1 cut(s) 659
Hin6I GCGC 3 cut(s) 421, 560, 693
HinP1I GCGC 3 cut(s) 421, 560, 693
HinfI GANTC 3 cut(s) 74, 195, 645
HpaII CCGG 4 cut(s) 171, 605, 820, 848
HphI GGTGA 3 cut(s) 41, 97, 724
Hpy166II GTNNAC 4 cut(s) 178, 220, 254, 520
Hpy188I TCNGA 4 cut(s) 189, 194, 378, 613
Hpy188III TCNNGA 7 cut(s) 260, 273, 298, 440, 680, 704, 793
Hpy8I GTNNAC 4 cut(s) 178, 220, 254, 520
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 4 cut(s) 120, 217, 538, 662
HpyCH4IV ACGT 4 cut(s) 277, 531, 570, 804
HpyCH4V TGCA 4 cut(s) 125, 323, 634, 777
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 614, 750, 816
HpyF3I CTNAG 1 cut(s) 791
HpySE526I ACGT 4 cut(s) 277, 531, 570, 804
Hsp92II CATG 1 cut(s) 659
HspAI GCGC 3 cut(s) 421, 560, 693
KroI GCCGGC 1 cut(s) 819
KroNI GCCGGC 1 cut(s) 821
Kzo9I GATC 3 cut(s) 189, 729, 795
Lsp1109I GCAGC 4 cut(s) 174, 276, 435, 646
LweI GCATC 4 cut(s) 111, 617, 697, 833
MaeI CTAG 2 cut(s) 369, 411
MaeII ACGT 4 cut(s) 277, 531, 570, 804
MaeIII GTNAC 3 cut(s) 211, 393, 532
MalI GATC 3 cut(s) 191, 731, 797
MboI GATC 3 cut(s) 189, 729, 795
MboII GAAGA 2 cut(s) 455, 587
MlsI TGGCCA 2 cut(s) 22, 347
MluCI AATT 5 cut(s) 34, 488, 507, 554, 580
MluNI TGGCCA 2 cut(s) 22, 347
MlyI GAGTC 1 cut(s) 639
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 5 cut(s) 267, 294, 300, 330, 333
Mox20I TGGCCA 2 cut(s) 22, 347
MroNI GCCGGC 1 cut(s) 819
MscI TGGCCA 2 cut(s) 22, 347
MseI TTAA 2 cut(s) 285, 566
MslI CAYNNNNRTG 1 cut(s) 828
Msp20I TGGCCA 2 cut(s) 22, 347
MspA1I CMGCKG 1 cut(s) 65
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 4 cut(s) 171, 605, 820, 848
MspR9I CCNGG 2 cut(s) 848, 849
Mva1269I GAATGC 1 cut(s) 617
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 4 cut(s) 171, 614, 750, 816
NaeI GCCGGC 1 cut(s) 821
NciI CCSGG 2 cut(s) 848, 849
NdeII GATC 3 cut(s) 189, 729, 795
NgoMIV GCCGGC 1 cut(s) 819
NlaIII CATG 1 cut(s) 659
NlaIV GGNNCC 1 cut(s) 824
NsbI TGCGCA 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 298
PctI GAATGC 1 cut(s) 617
PdiI GCCGGC 1 cut(s) 821
PfeI GAWTC 2 cut(s) 74, 195
PkrI GCNGC 6 cut(s) 61, 64, 164, 291, 425, 636
PleI GAGTC 1 cut(s) 639
PpsI GAGTC 1 cut(s) 639
PspN4I GGNNCC 1 cut(s) 824
PspPI GGNCC 1 cut(s) 204
RsaI GTAC 4 cut(s) 280, 353, 468, 519
RsaNI GTAC 4 cut(s) 279, 352, 467, 518
RseI CAYNNNNRTG 1 cut(s) 828
SaqAI TTAA 2 cut(s) 285, 566
SatI GCNGC 6 cut(s) 60, 63, 163, 290, 424, 635
Sau3AI GATC 3 cut(s) 189, 729, 795
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 1 cut(s) 639
ScrFI CCNGG 2 cut(s) 848, 849
SfaNI GCATC 4 cut(s) 111, 617, 697, 833
SfcI CTRYAG 1 cut(s) 586
Sfr274I CTCGAG 1 cut(s) 298
SlaI CTCGAG 1 cut(s) 298
SmaI CCCGGG 1 cut(s) 849
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 2 cut(s) 284, 298
SmoI CTYRAG 2 cut(s) 284, 298
Sse9I AATT 5 cut(s) 34, 488, 507, 554, 580
SsiI CCGC 5 cut(s) 60, 63, 160, 292, 716
SspMI CTAG 2 cut(s) 369, 411
StyD4I CCNGG 2 cut(s) 846, 847
StyI CCWWGG 2 cut(s) 207, 238
TaaI ACNGT 4 cut(s) 120, 217, 538, 662
TaiI ACGT 4 cut(s) 280, 534, 573, 807
TaqI TCGA 3 cut(s) 299, 513, 783
TasI AATT 5 cut(s) 34, 488, 507, 554, 580
TatI WGTACW 3 cut(s) 351, 466, 517
TauI GCSGC 2 cut(s) 62, 65
TfiI GAWTC 2 cut(s) 74, 195
Tru1I TTAA 2 cut(s) 285, 566
Tru9I TTAA 2 cut(s) 285, 566
TscAI CASTG 1 cut(s) 85
TseI GCWGC 4 cut(s) 162, 289, 423, 634
TspDTI ATGAA 4 cut(s) 110, 125, 416, 610
TspGWI ACGGA 2 cut(s) 347, 395
TspMI CCCGGG 1 cut(s) 847
TspRI CASTG 1 cut(s) 85
Vha464I CTTAAG 1 cut(s) 284
XapI RAATTY 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 847
XspI CTAG 2 cut(s) 369, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.