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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 236 |
| AciI | CCGC | 1 cut(s) | 236 |
| AcsI | RAATTY | 1 cut(s) | 5 |
| AgsI | TTSAA | 2 cut(s) | 11, 214 |
| AoxI | GGCC | 1 cut(s) | 61 |
| ApoI | RAATTY | 1 cut(s) | 5 |
| AspLEI | GCGC | 1 cut(s) | 205 |
| BbsI | GAAGAC | 1 cut(s) | 99 |
| BccI | CCATC | 1 cut(s) | 85 |
| BfaI | CTAG | 1 cut(s) | 161 |
| BisI | GCNGC | 1 cut(s) | 237 |
| BlsI | GCNGC | 1 cut(s) | 238 |
| BmiI | GGNNCC | 1 cut(s) | 147 |
| BpiI | GAAGAC | 1 cut(s) | 99 |
| BsaJI | CCNNGG | 1 cut(s) | 175 |
| BseDI | CCNNGG | 1 cut(s) | 175 |
| BseYI | CCCAGC | 1 cut(s) | 126 |
| BshFI | GGCC | 1 cut(s) | 63 |
| BsnI | GGCC | 1 cut(s) | 63 |
| BspACI | CCGC | 1 cut(s) | 236 |
| BspANI | GGCC | 1 cut(s) | 63 |
| BspLI | GGNNCC | 1 cut(s) | 147 |
| BsrBI | CCGCTC | 1 cut(s) | 236 |
| BssECI | CCNNGG | 1 cut(s) | 175 |
| BssT1I | CCWWGG | 1 cut(s) | 175 |
| BstC8I | GCNNGC | 1 cut(s) | 187 |
| BstHHI | GCGC | 1 cut(s) | 205 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 191, 200 |
| BstV2I | GAAGAC | 1 cut(s) | 99 |
| BsuRI | GGCC | 1 cut(s) | 63 |
| BtsI | GCAGTG | 1 cut(s) | 66 |
| BtsIMutI | CAGTG | 1 cut(s) | 66 |
| Cac8I | GCNNGC | 1 cut(s) | 187 |
| CfoI | GCGC | 1 cut(s) | 205 |
| CviAII | CATG | 1 cut(s) | 142 |
| CviJI | RGCY | 5 cut(s) | 63, 130, 146, 159, 239 |
| CviKI_1 | RGCY | 5 cut(s) | 63, 130, 146, 159, 239 |
| Eco130I | CCWWGG | 1 cut(s) | 175 |
| EcoT14I | CCWWGG | 1 cut(s) | 175 |
| ErhI | CCWWGG | 1 cut(s) | 175 |
| FaeI | CATG | 1 cut(s) | 145 |
| FaiI | YATR | 1 cut(s) | 143 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 159, 191 |
| FatI | CATG | 1 cut(s) | 141 |
| Fnu4HI | GCNGC | 1 cut(s) | 237 |
| Fsp4HI | GCNGC | 1 cut(s) | 237 |
| FspBI | CTAG | 1 cut(s) | 161 |
| GlaI | GCGC | 1 cut(s) | 204 |
| GluI | GCNGC | 1 cut(s) | 237 |
| GsaI | CCCAGC | 1 cut(s) | 130 |
| HaeIII | GGCC | 1 cut(s) | 63 |
| HhaI | GCGC | 1 cut(s) | 205 |
| Hin1II | CATG | 1 cut(s) | 145 |
| Hin6I | GCGC | 1 cut(s) | 203 |
| HinP1I | GCGC | 1 cut(s) | 203 |
| Hpy188I | TCNGA | 4 cut(s) | 82, 91, 150, 233 |
| HpyCH4IV | ACGT | 1 cut(s) | 111 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 191, 200 |
| HpySE526I | ACGT | 1 cut(s) | 111 |
| Hsp92II | CATG | 1 cut(s) | 145 |
| HspAI | GCGC | 1 cut(s) | 203 |
| LmnI | GCTCC | 1 cut(s) | 151 |
| LpnPI | CCDG | 2 cut(s) | 140, 199 |
| MaeI | CTAG | 1 cut(s) | 161 |
| MaeII | ACGT | 1 cut(s) | 111 |
| MbiI | CCGCTC | 1 cut(s) | 236 |
| MboII | GAAGA | 1 cut(s) | 104 |
| MluCI | AATT | 1 cut(s) | 5 |
| MnlI | CCTC | 4 cut(s) | 33, 51, 74, 125 |
| MseI | TTAA | 2 cut(s) | 137, 241 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 191, 200 |
| NlaIII | CATG | 1 cut(s) | 145 |
| NlaIV | GGNNCC | 1 cut(s) | 147 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 108 |
| PflFI | GACNNNGTC | 1 cut(s) | 110 |
| PkrI | GCNGC | 1 cut(s) | 238 |
| PspFI | CCCAGC | 1 cut(s) | 126 |
| PspN4I | GGNNCC | 1 cut(s) | 147 |
| PsyI | GACNNNGTC | 1 cut(s) | 110 |
| SaqAI | TTAA | 2 cut(s) | 137, 241 |
| SatI | GCNGC | 1 cut(s) | 237 |
| SetI | ASST | 3 cut(s) | 99, 114, 177 |
| Sse9I | AATT | 1 cut(s) | 5 |
| SsiI | CCGC | 1 cut(s) | 236 |
| SspMI | CTAG | 1 cut(s) | 161 |
| StyI | CCWWGG | 1 cut(s) | 175 |
| TaiI | ACGT | 1 cut(s) | 114 |
| TaqI | TCGA | 1 cut(s) | 155 |
| TasI | AATT | 1 cut(s) | 5 |
| TauI | GCSGC | 1 cut(s) | 239 |
| Tru1I | TTAA | 2 cut(s) | 137, 241 |
| Tru9I | TTAA | 2 cut(s) | 137, 241 |
| TscAI | CASTG | 1 cut(s) | 73 |
| TspRI | CASTG | 1 cut(s) | 73 |
| Tth111I | GACNNNGTC | 1 cut(s) | 110 |
| XapI | RAATTY | 1 cut(s) | 5 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 56, 139, 173 |
| XspI | CTAG | 1 cut(s) | 161 |