Rh6BG060700

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000672
Physical Location & Seq
Reverse (-)
9619645 .. 9620415
771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG060700.1

Sequence Viewer

Length: 771 bp
ATGAGCAGCATCACCGATGAGAACCTTGAATTTGAATTGGAGGAGACAGACATATTTGATACCATAGTCTATCAGGAGGAAACGAACTGCTGCTACAGAAGCGGCGCCGGCGTGGACATACTGGCGATCAGATTCTGCCTCTACGAGGTAATTAAATACCGTAATAGATTCGAAGAATTTGAGTTGGAACCAGGCGTTTTATTAGCTCGTCATGTACTTAAGTTGCGGATCTCGAGAGAGAGGAGCAGTTGGGATTCAAATAAAAGGATGGACAGGTTGGCTAGGTACATAGGGATCATAGCTAGGTCTCCAAGGGAGAAGAGAAGGTTTAAGAGGAAGATGGTTAGGTATCTTTCAATGATTGGAGCTCCGGAAGAGGAGCACGAGTCTATTATTGAAAATATATTGGAGGAGCTTAAAGATGCTGTCCCCGACTTGATACTTGATGTGCAGATGACGAAATTCATAACTAGATACACGGCCGAGTATTTCTTCGACGACACAGACTACAGGGACAAGCTAATTCCTGCAACCCAATCATTTATCCAGGGTTTGGAGAAAGTGACATCACTAGAGAGTTTTGGCGGACAGACTTGTGCTATTTGTAGGGAGGCATTTGCTGCACATCATCATCTGAATCGGTTGCCCTGCTTGCACTCTTATCATCAACGTTGCATTGTCCAGTGGCTCCAAATCAATCACGTCTGCCCCATTTGTAGACACCCTTATGCTACTGCCTCTGCCGAGCCATCAGACCCTAATCCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

256

Amino Acids

30.0

Weight (kDa)

5.66

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 198 - 240 4.7e-11 Ring finger domain
zf-C3HC4_2 PF13923 199 - 239 5.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 199 - 239 9.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 553
AccI GTMKAC 1 cut(s) 718
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 3 cut(s) 102, 226, 585
AclI AACGTT 1 cut(s) 670
AclWI GGATC 2 cut(s) 236, 302
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 3 cut(s) 29, 176, 461
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 2 cut(s) 216, 287
AfiI CCNNNNNNNGG 2 cut(s) 145, 553
AflII CTTAAG 1 cut(s) 218
AgsI TTSAA 5 cut(s) 29, 35, 258, 357, 398
AjiI CACGTC 1 cut(s) 703
AjnI CCWGG 2 cut(s) 190, 546
AluBI AGCT 5 cut(s) 206, 302, 368, 415, 520
AluI AGCT 5 cut(s) 206, 302, 368, 415, 520
Alw21I GWGCWC 2 cut(s) 370, 384
Alw26I GTCTC 2 cut(s) 38, 312
AlwI GGATC 2 cut(s) 236, 302
AlwNI CAGNNNCTG 1 cut(s) 135
Ama87I CYCGRG 1 cut(s) 232
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 1 cut(s) 480
ApeKI GCWGC 3 cut(s) 6, 90, 620
ApoI RAATTY 3 cut(s) 29, 176, 461
AspLEI GCGC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 4
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 232
BaeI ACNNNNGTAYC 2 cut(s) 51, 84
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 1 cut(s) 370
BauI CACGAG 1 cut(s) 383
Bbv12I GWGCWC 2 cut(s) 370, 384
BbvI GCAGC 3 cut(s) 18, 77, 607
BccI CCATC 3 cut(s) 262, 334, 757
BceAI ACGGC 1 cut(s) 495
BciT130I CCWGG 2 cut(s) 192, 548
BcoDI GTCTC 2 cut(s) 38, 312
BfaI CTAG 4 cut(s) 282, 303, 471, 572
BfmI CTRYAG 2 cut(s) 94, 508
BfoI RGCGCY 1 cut(s) 108
BfrI CTTAAG 1 cut(s) 218
BisI GCNGC 4 cut(s) 7, 91, 103, 621
BlsI GCNGC 4 cut(s) 8, 92, 104, 622
Bme1390I CCNGG 2 cut(s) 192, 548
BmeT110I CYCGRG 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 703
BmiI GGNNCC 3 cut(s) 106, 189, 689
BmrFI CCNGG 2 cut(s) 192, 548
BmsI GCATC 2 cut(s) 18, 412
Bpu14I TTCGAA 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 311, 547
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 553
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 126, 682
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 2 cut(s) 192, 548
BseDI CCNNGG 2 cut(s) 311, 547
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 145, 553
BseNI ACTGG 2 cut(s) 126, 682
BseRI GAGGAG 4 cut(s) 56, 256, 392, 425
BseX3I CGGCCG 1 cut(s) 480
BseXI GCAGC 3 cut(s) 18, 77, 607
BsgI GTGCAG 2 cut(s) 470, 606
Bsh1285I CGRYCG 1 cut(s) 483
BshFI GGCC 1 cut(s) 482
BshNI GGYRCC 1 cut(s) 104
BsiEI CGRYCG 1 cut(s) 483
BsiHKAI GWGCWC 2 cut(s) 370, 384
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 2 cut(s) 108, 371
BslFI GGGAC 2 cut(s) 413, 527
BslI CCNNNNNNNGG 2 cut(s) 145, 553
BsmAI GTCTC 2 cut(s) 38, 312
BsmFI GGGAC 2 cut(s) 413, 527
BsnI GGCC 1 cut(s) 482
Bso31I GGTCTC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 232
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 370, 384
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 3 cut(s) 126, 228, 294
BspACI CCGC 3 cut(s) 102, 226, 585
BspANI GGCC 1 cut(s) 482
BspEI TCCGGA 1 cut(s) 370
BspLI GGNNCC 3 cut(s) 106, 189, 689
BspPI GGATC 2 cut(s) 236, 302
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 104
BspTI CTTAAG 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 2 cut(s) 126, 682
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 311, 547
BssMI GATC 3 cut(s) 126, 228, 294
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 383
BssT1I CCWWGG 1 cut(s) 311
Bst2BI CACGAG 1 cut(s) 383
Bst2UI CCWGG 2 cut(s) 192, 548
Bst4CI ACNGT 1 cut(s) 161
Bst6I CTCTTC 2 cut(s) 314, 369
BstACI GRCGYC 1 cut(s) 105
BstAFI CTTAAG 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 109, 653
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 273
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 3 cut(s) 129, 231, 297
BstMAI GTCTC 2 cut(s) 38, 312
BstMBI GATC 3 cut(s) 126, 228, 294
BstMCI CGRYCG 1 cut(s) 483
BstMWI GCNNNNNNNGC 4 cut(s) 99, 108, 620, 652
BstNI CCWGG 2 cut(s) 192, 548
BstSCI CCNGG 2 cut(s) 190, 546
BstSFI CTRYAG 2 cut(s) 94, 508
BstV1I GCAGC 3 cut(s) 18, 77, 607
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
BstZI CGGCCG 1 cut(s) 480
BsuRI GGCC 1 cut(s) 482
BtrI CACGTC 1 cut(s) 703
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 689
Cac8I GCNNGC 2 cut(s) 109, 653
CaiI CAGNNNCTG 1 cut(s) 135
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 107
Csp6I GTAC 2 cut(s) 215, 286
CviAII CATG 1 cut(s) 212
CviJI RGCY 9 cut(s) 206, 281, 302, 368, 415, 482, 520, 688, 748
CviKI_1 RGCY 9 cut(s) 206, 281, 302, 368, 415, 482, 520, 688, 748
CviQI GTAC 2 cut(s) 215, 286
DinI GGCGCC 1 cut(s) 106
DpnI GATC 3 cut(s) 128, 230, 296
DpnII GATC 3 cut(s) 126, 228, 294
EaeI YGGCCR 1 cut(s) 480
EagI CGGCCG 1 cut(s) 480
Eam1104I CTCTTC 2 cut(s) 314, 369
EarI CTCTTC 2 cut(s) 314, 369
EciI GGCGGA 1 cut(s) 600
Ecl136II GAGCTC 1 cut(s) 368
EclXI CGGCCG 1 cut(s) 480
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 370
Eco31I GGTCTC 1 cut(s) 312
Eco52I CGGCCG 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 368
Eco88I CYCGRG 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 190, 546
EcoT14I CCWWGG 1 cut(s) 311
EcoT38I GRGCYC 1 cut(s) 370
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 1 cut(s) 215
FaiI YATR 9 cut(s) 53, 65, 119, 213, 290, 299, 404, 467, 729
FaqI GGGAC 2 cut(s) 413, 527
FatI CATG 1 cut(s) 211
FblI GTMKAC 1 cut(s) 718
Fnu4HI GCNGC 4 cut(s) 7, 91, 103, 621
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 4 cut(s) 7, 91, 103, 621
FspBI CTAG 4 cut(s) 282, 303, 471, 572
GlaI GCGC 1 cut(s) 106
GluI GCNGC 4 cut(s) 7, 91, 103, 621
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 1 cut(s) 482
HapII CCGG 2 cut(s) 108, 371
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 1 cut(s) 215
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 5 cut(s) 132, 168, 254, 386, 637
HpaII CCGG 2 cut(s) 108, 371
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 2 cut(s) 115, 719
Hpy188I TCNGA 3 cut(s) 131, 636, 754
Hpy188III TCNNGA 5 cut(s) 74, 232, 234, 371, 764
Hpy8I GTNNAC 2 cut(s) 115, 719
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 1 cut(s) 318
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 2 cut(s) 670, 702
HpyCH4V TGCA 5 cut(s) 451, 530, 623, 655, 675
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 108, 620, 652
HpySE526I ACGT 2 cut(s) 670, 702
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 1 cut(s) 215
HspAI GCGC 1 cut(s) 105
KasI GGCGCC 1 cut(s) 104
Kpn2I TCCGGA 1 cut(s) 370
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Kzo9I GATC 3 cut(s) 126, 228, 294
LmnI GCTCC 6 cut(s) 243, 365, 373, 379, 412, 693
Lsp1109I GCAGC 3 cut(s) 18, 77, 607
LweI GCATC 2 cut(s) 18, 412
MaeI CTAG 4 cut(s) 282, 303, 471, 572
MaeII ACGT 2 cut(s) 670, 702
MaeIII GTNAC 1 cut(s) 562
MalI GATC 3 cut(s) 128, 230, 296
MboI GATC 3 cut(s) 126, 228, 294
MboII GAAGA 5 cut(s) 185, 331, 349, 386, 484
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 370, 384
MluCI AATT 6 cut(s) 29, 35, 150, 176, 461, 522
Mly113I GGCGCC 1 cut(s) 105
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 165
MreI CGCCGGCG 1 cut(s) 107
MroI TCCGGA 1 cut(s) 370
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 4 cut(s) 153, 219, 330, 417
MslI CAYNNNNRTG 1 cut(s) 726
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 2 cut(s) 108, 371
MspR9I CCNGG 2 cut(s) 192, 548
MvaI CCWGG 2 cut(s) 192, 548
MwoI GCNNNNNNNGC 4 cut(s) 99, 108, 620, 652
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 105
NdeII GATC 3 cut(s) 126, 228, 294
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 1 cut(s) 215
NlaIV GGNNCC 3 cut(s) 106, 189, 689
NmeAIII GCCGAG 2 cut(s) 508, 769
NmuCI GTSAC 1 cut(s) 562
NspV TTCGAA 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 4 cut(s) 132, 168, 254, 637
PflMI CCANNNNNTGG 1 cut(s) 553
PkrI GCNGC 4 cut(s) 8, 92, 104, 622
PleI GAGTC 1 cut(s) 394
PluTI GGCGCC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 370
Psp1406I AACGTT 1 cut(s) 670
Psp6I CCWGG 2 cut(s) 190, 546
PspGI CCWGG 2 cut(s) 190, 546
PspN4I GGNNCC 3 cut(s) 106, 189, 689
PsrI GAACNNNNNNTAC 2 cut(s) 77, 109
PstNI CAGNNNCTG 1 cut(s) 135
PsuI RGATCY 1 cut(s) 228
RsaI GTAC 2 cut(s) 216, 287
RsaNI GTAC 2 cut(s) 215, 286
RseI CAYNNNNRTG 1 cut(s) 726
SacI GAGCTC 1 cut(s) 370
SaqAI TTAA 4 cut(s) 153, 219, 330, 417
SatI GCNGC 4 cut(s) 7, 91, 103, 621
Sau3AI GATC 3 cut(s) 126, 228, 294
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 2 cut(s) 192, 548
SduI GDGCHC 2 cut(s) 370, 384
SfaNI GCATC 2 cut(s) 18, 412
SfcI CTRYAG 2 cut(s) 94, 508
SfoI GGCGCC 1 cut(s) 106
Sfr274I CTCGAG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 171
SgrAI CRCCGGYG 1 cut(s) 107
SlaI CTCGAG 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 2 cut(s) 218, 232
SmoI CTYRAG 2 cut(s) 218, 232
Sse9I AATT 6 cut(s) 29, 35, 150, 176, 461, 522
SsiI CCGC 3 cut(s) 102, 226, 585
SspDI GGCGCC 1 cut(s) 104
SspMI CTAG 4 cut(s) 282, 303, 471, 572
SstI GAGCTC 1 cut(s) 370
StyD4I CCNGG 2 cut(s) 190, 546
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 2 cut(s) 673, 705
TaqI TCGA 3 cut(s) 171, 233, 495
TasI AATT 6 cut(s) 29, 35, 150, 176, 461, 522
TatI WGTACW 1 cut(s) 214
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 4 cut(s) 132, 168, 254, 637
Tru1I TTAA 4 cut(s) 153, 219, 330, 417
Tru9I TTAA 4 cut(s) 153, 219, 330, 417
TscAI CASTG 1 cut(s) 689
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 3 cut(s) 6, 90, 620
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 1 cut(s) 454
TspRI CASTG 1 cut(s) 689
Van91I CCANNNNNTGG 1 cut(s) 553
Vha464I CTTAAG 1 cut(s) 218
XagI CCTNNNNNAGG 1 cut(s) 143
XapI RAATTY 3 cut(s) 29, 176, 461
XhoI CTCGAG 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 718
XspI CTAG 4 cut(s) 282, 303, 471, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.