FvH4_3g25280

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000346
Physical Location & Seq
Forward (+)
18250729 .. 18251289
561 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25280.t1

Sequence Viewer

Length: 561 bp
ATGGCAATGGCTGACGAGGACATTCTTTCGGATTTCATTAAGCCTACTCCAAATGCCACAATAGATGCAAACTACTTCACATTCACCGGCATGCGTGTTCTTGTTGGAGGAACACCTCCCGCAACCTTCACAGTGTTGAAGGCAAGCATGGCGGAGTTCCCTGCTCTTTTCGGGCAGAGTGTTTCGTACGCTGTCCTTCAATTCCCATCTGGCACAGTTAACCCACCACACACTCATCCTCGCTCTGCTGAGCTCCTCTTCCTTGTTGACGGTACCCTTGAAGTGGGATTTGTTGACACAAAGAACAACCTCTTTACTCAGACTATCCAAGCTGGTGACCTGTTTGTGTTTCCCAAGGGACTTGTGCACTACCAGTACAATGCTGATGCACAGAACCCGGCTCTAGCCATTTCAGCCTTTGGAAGTGCAAATGCTGGAACTGTATCAATTCCTTCCACTTTGTTCACCACCAATATTGATGATAATGTCCTTGCTTTGTCACTCAAGACCGATGTAGCCACCGTTCAGAAACTCAAGGCTGGACTTGCTCCGAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

187

Amino Acids

19.74

Weight (kDa)

4.94

Isoelectric Point (pI)

25.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 48 - 175 1.2e-30 Cupin
Cupin_2 PF07883 66 - 138 4.9e-12 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 272
AciI CCGC 2 cut(s) 120, 152
AfaI GTAC 3 cut(s) 188, 274, 377
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 3 cut(s) 139, 200, 281
AluBI AGCT 2 cut(s) 253, 332
AluI AGCT 2 cut(s) 253, 332
Alw21I GWGCWC 2 cut(s) 255, 369
Alw44I GTGCAC 1 cut(s) 365
ApaLI GTGCAC 1 cut(s) 365
Asp718I GGTACC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 3 cut(s) 76, 347, 457
BaeGI GKGCMC 1 cut(s) 369
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 255, 369
BccI CCATC 1 cut(s) 214
BcnI CCSGG 1 cut(s) 398
BfaI CTAG 1 cut(s) 404
BlpI GCTNAGC 1 cut(s) 249
Bme1390I CCNGG 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 2 cut(s) 55, 376
Bpu1102I GCTNAGC 1 cut(s) 249
BpuEI CTTGAG 2 cut(s) 488, 518
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 373
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 240, 332
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 245
BseSI GKGCMC 1 cut(s) 369
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 2 cut(s) 255, 369
BsiSI CCGG 2 cut(s) 87, 398
BsiWI CGTACG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 255, 369
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 2 cut(s) 120, 152
BspCNI CTCAG 2 cut(s) 241, 331
BspLI GGNNCC 1 cut(s) 274
BspT107I GGYRCC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 354
Bst4CI ACNGT 5 cut(s) 133, 217, 272, 442, 523
Bst6I CTCTTC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 92, 145
BstDEI CTNAG 2 cut(s) 249, 318
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 149, 413, 545
BstNSI RCATGY 1 cut(s) 94
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 92, 145
Cfr10I RCCGGY 1 cut(s) 86
Csp6I GTAC 3 cut(s) 187, 273, 376
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 3 cut(s) 91, 148, 558
CviQI GTAC 3 cut(s) 187, 273, 376
DdeI CTNAG 2 cut(s) 249, 318
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 255
Eco53kI GAGCTC 1 cut(s) 253
Eco91I GGTNACC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 3 cut(s) 94, 151, 561
FaiI YATR 3 cut(s) 92, 149, 559
FaqI GGGAC 1 cut(s) 372
FatI CATG 3 cut(s) 90, 147, 557
FauI CCCGC 1 cut(s) 127
FokI GGATG 1 cut(s) 222
FriOI GRGCYC 1 cut(s) 255
FspBI CTAG 1 cut(s) 404
HapII CCGG 2 cut(s) 87, 398
Hin1II CATG 3 cut(s) 94, 151, 561
HincII GTYRAC 3 cut(s) 220, 268, 295
HindII GTYRAC 3 cut(s) 220, 268, 295
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 2 cut(s) 87, 398
HphI GGTGA 3 cut(s) 76, 347, 457
Hpy166II GTNNAC 5 cut(s) 220, 268, 295, 367, 465
Hpy188I TCNGA 4 cut(s) 31, 321, 528, 552
Hpy188III TCNNGA 1 cut(s) 505
Hpy8I GTNNAC 5 cut(s) 220, 268, 295, 367, 465
HpyAV CCTTC 4 cut(s) 133, 136, 206, 462
HpyCH4III ACNGT 5 cut(s) 133, 217, 272, 442, 523
HpyCH4V TGCA 4 cut(s) 68, 367, 389, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 413, 545
HpyF3I CTNAG 2 cut(s) 249, 318
Hsp92II CATG 3 cut(s) 94, 151, 561
KpnI GGTACC 1 cut(s) 276
KspAI GTTAAC 1 cut(s) 220
LmnI GCTCC 2 cut(s) 258, 553
LpnPI CCDG 9 cut(s) 100, 174, 195, 318, 353, 386, 411, 420, 525
LweI GCATC 2 cut(s) 55, 376
MaeI CTAG 1 cut(s) 404
MaeIII GTNAC 2 cut(s) 335, 498
MboII GAAGA 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 255, 369
MluCI AATT 2 cut(s) 200, 447
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 6 cut(s) 10, 101, 126, 249, 266, 320
MseI TTAA 2 cut(s) 39, 219
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 2 cut(s) 87, 398
MspR9I CCNGG 1 cut(s) 398
MwoI GCNNNNNNNGC 3 cut(s) 149, 413, 545
NciI CCSGG 1 cut(s) 398
NlaIII CATG 3 cut(s) 94, 151, 561
NlaIV GGNNCC 1 cut(s) 274
NmuCI GTSAC 2 cut(s) 335, 498
NspI RCATGY 1 cut(s) 94
PaeI GCATGC 1 cut(s) 94
Pfl23II CGTACG 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 335
PspLI CGTACG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 274
PsrI GAACNNNNNNTAC 2 cut(s) 507, 539
RsaI GTAC 3 cut(s) 188, 274, 377
RsaNI GTAC 3 cut(s) 187, 273, 376
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 39, 219
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 2 cut(s) 255, 369
SetI ASST 6 cut(s) 118, 128, 255, 312, 334, 342
SfaNI GCATC 2 cut(s) 55, 376
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 503, 533
SmoI CTYRAG 2 cut(s) 503, 533
SphI GCATGC 1 cut(s) 94
Sse9I AATT 2 cut(s) 200, 447
SsiI CCGC 2 cut(s) 120, 152
SspI AATATT 1 cut(s) 475
SspMI CTAG 1 cut(s) 404
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 5 cut(s) 133, 217, 272, 442, 523
TaqII GACCGA 1 cut(s) 524
TasI AATT 2 cut(s) 200, 447
TatI WGTACW 1 cut(s) 375
Tru1I TTAA 2 cut(s) 39, 219
Tru9I TTAA 2 cut(s) 39, 219
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 2 cut(s) 335, 498
Tsp45I GTSAC 2 cut(s) 335, 498
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 1 cut(s) 138
VneI GTGCAC 1 cut(s) 365
XceI RCATGY 1 cut(s) 94
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.