Rroxscaffold_1G00034550

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000346
Physical Location & Seq
Reverse (-)
50189563 .. 50190187
625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00034550.1

Sequence Viewer

Length: 522 bp
ATGGCCTCGAGAACTTCAACCCTAGCATTCTTCTCACTAATAATCTGTTCTCTTGCAATCCCTGAAATGGCAATGGCCGGAGATCCAGATATTCTTAGTGACTTCATTGCGCCCCCAAATGGAACAGTAGATGGAAAATTCTTCACTTACACTGGCATGCGTGTTATCTTTCAAGATGATCCCAAAAACTTCACAGTTCTGAAGGCAACCTCCGCTGAGTTCCCTGCTCTTAATGGTCAGAGTGTTTCATATGCAGTACTTGAATTCCCTTCTGGCACTACTAACCCACCTCACACTCATCCTCGCTCTGCTGAGCTCCTCTTCCTTGTTGGCGGTTCCCTTGAAGTTGGCTTTGTCGACACAAAAAACAACCTCTTTACTCAGACTCTTCAGGTAGGAACTGTGTCAATTCCTTCCACTTTGTTCGCCACCAACATCGATGACGATGTCTTGGCTTTGTCCTTCAAGACTGACGTATCTACCATTCAAAAGCTTAAGGCTGGTCTAGCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

173

Amino Acids

18.48

Weight (kDa)

4.82

Isoelectric Point (pI)

20.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 61 - 164 8.1e-15 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 357
AciI CCGC 2 cut(s) 213, 333
AclWI GGATC 2 cut(s) 77, 173
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 2 cut(s) 137, 263
AcuI CTGAAG 2 cut(s) 221, 374
AfaI GTAC 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 67, 119
AflII CTTAAG 1 cut(s) 494
AgsI TTSAA 6 cut(s) 18, 173, 263, 344, 466, 488
AluBI AGCT 3 cut(s) 316, 493, 509
AluI AGCT 3 cut(s) 316, 493, 509
Alw21I GWGCWC 1 cut(s) 318
AlwI GGATC 2 cut(s) 77, 173
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 2 cut(s) 3, 75
ApoI RAATTY 2 cut(s) 137, 263
AspLEI GCGC 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 7
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BanII GRGCYC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 318
BccI CCATC 1 cut(s) 125
BfaI CTAG 2 cut(s) 23, 506
BfrI CTTAAG 1 cut(s) 494
BlpI GCTNAGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 258
BmeT110I CYCGRG 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 337
Bpu1102I GCTNAGC 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 438
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 119
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 2 cut(s) 78, 105
BseCI ATCGAT 1 cut(s) 438
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 2 cut(s) 67, 119
BseMI GCAATG 2 cut(s) 78, 105
BseMII CTCAG 3 cut(s) 207, 303, 395
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 308
BshFI GGCC 2 cut(s) 5, 77
BshVI ATCGAT 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 1 cut(s) 78
BslI CCNNNNNNNGG 2 cut(s) 67, 119
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 5, 77
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 2 cut(s) 82, 178
Bsp1720I GCTNAGC 1 cut(s) 312
BspACI CCGC 2 cut(s) 213, 333
BspANI GGCC 2 cut(s) 5, 77
BspCNI CTCAG 3 cut(s) 208, 304, 394
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 337
BspPI GGATC 2 cut(s) 77, 173
BspTI CTTAAG 1 cut(s) 494
BsrDI GCAATG 2 cut(s) 78, 105
BsrI ACTGG 1 cut(s) 157
BssMI GATC 2 cut(s) 82, 178
Bst4CI ACNGT 3 cut(s) 127, 196, 403
Bst6I CTCTTC 2 cut(s) 326, 393
BstAFI CTTAAG 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 4 cut(s) 95, 216, 312, 381
BstF5I GGATG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 85, 181
BstMBI GATC 2 cut(s) 82, 178
BstMWI GCNNNNNNNGC 2 cut(s) 212, 506
BstNSI RCATGY 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
Bsu15I ATCGAT 1 cut(s) 438
BsuRI GGCC 2 cut(s) 5, 77
BsuTUI ATCGAT 1 cut(s) 438
BtsCI GGATG 1 cut(s) 298
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 158
CfoI GCGC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 438
Csp6I GTAC 1 cut(s) 257
CviAII CATG 2 cut(s) 157, 519
CviJI RGCY 9 cut(s) 5, 77, 316, 351, 455, 493, 500, 509, 517
CviKI_1 RGCY 9 cut(s) 5, 77, 316, 351, 455, 493, 500, 509, 517
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 4 cut(s) 95, 216, 312, 381
DpnI GATC 2 cut(s) 84, 180
DpnII GATC 2 cut(s) 82, 178
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 326, 393
EarI CTCTTC 2 cut(s) 326, 393
Ecl136II GAGCTC 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 318
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 221, 374
Eco88I CYCGRG 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 318
FaeI CATG 2 cut(s) 160, 522
FaiI YATR 4 cut(s) 158, 250, 252, 520
FatI CATG 2 cut(s) 156, 518
FauNDI CATATG 1 cut(s) 250
FblI GTMKAC 1 cut(s) 357
FokI GGATG 1 cut(s) 285
FriOI GRGCYC 1 cut(s) 318
FspBI CTAG 2 cut(s) 23, 506
GlaI GCGC 1 cut(s) 111
HaeIII GGCC 2 cut(s) 5, 77
HapII CCGG 1 cut(s) 78
HhaI GCGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 160, 522
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 78
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 3 cut(s) 201, 240, 384
Hpy188III TCNNGA 4 cut(s) 9, 86, 173, 466
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 4 cut(s) 196, 279, 423, 472
HpyCH4III ACNGT 3 cut(s) 127, 196, 403
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 2 cut(s) 56, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 506
HpyF3I CTNAG 4 cut(s) 95, 216, 312, 381
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 2 cut(s) 160, 522
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 2 cut(s) 82, 178
LmnI GCTCC 2 cut(s) 321, 514
LpnPI CCDG 8 cut(s) 75, 91, 99, 138, 237, 258, 377, 486
MaeI CTAG 2 cut(s) 23, 506
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 1 cut(s) 98
MalI GATC 2 cut(s) 84, 180
MboI GATC 2 cut(s) 82, 178
MboII GAAGA 4 cut(s) 22, 133, 313, 380
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 3 cut(s) 137, 263, 408
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 6 cut(s) 16, 220, 300, 312, 329, 383
MseI TTAA 2 cut(s) 231, 495
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 494
MspI CCGG 1 cut(s) 78
Mva1269I GAATGC 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 212, 506
NdeI CATATG 1 cut(s) 250
NdeII GATC 2 cut(s) 82, 178
NlaIII CATG 2 cut(s) 160, 522
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 1 cut(s) 98
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PaeR7I CTCGAG 1 cut(s) 7
PctI GAATGC 1 cut(s) 26
PflFI GACNNNGTC 1 cut(s) 446
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 337
PsuI RGATCY 1 cut(s) 82
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 318
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 231, 495
Sau3AI GATC 2 cut(s) 82, 178
ScaI AGTACT 1 cut(s) 258
SchI GAGTC 1 cut(s) 379
SduI GDGCHC 1 cut(s) 318
SetI ASST 8 cut(s) 212, 292, 318, 375, 396, 477, 495, 511
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 7, 494
SmoI CTYRAG 2 cut(s) 7, 494
SphI GCATGC 1 cut(s) 160
Sse9I AATT 3 cut(s) 137, 263, 408
SsiI CCGC 2 cut(s) 213, 333
SspMI CTAG 2 cut(s) 23, 506
SstI GAGCTC 1 cut(s) 318
TaaI ACNGT 3 cut(s) 127, 196, 403
TaiI ACGT 1 cut(s) 477
TaqI TCGA 3 cut(s) 8, 357, 438
TasI AATT 3 cut(s) 137, 263, 408
TatI WGTACW 1 cut(s) 256
Tru1I TTAA 2 cut(s) 231, 495
Tru9I TTAA 2 cut(s) 231, 495
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 2 cut(s) 94, 237
TspRI CASTG 1 cut(s) 157
Tth111I GACNNNGTC 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 494
XapI RAATTY 2 cut(s) 137, 263
XceI RCATGY 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 2 cut(s) 23, 506
ZrmI AGTACT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.