Rh5DG331000

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000346
Physical Location & Seq
Forward (+)
43654791 .. 43655261
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG331000.1

Sequence Viewer

Length: 471 bp
ATGCGTGTTGTCTTTGAAGAAGATGAGCCTAAAAACTTCACAATTCTGAAGGCAACCTCCGCTGAGTTCCCTGCCCTTAATGGTCAGAGTGTTTCATATGCAGTACTTGAATTTCCATCTGGCATTACTAACCCACCTCACACTCATCCTCGCTCTGTTGAGCTCCTCTTCCTTGTTGACGGTACCCTTGAGGTTGGCTTTGTCGACACAAAAAACAACCTCTTTACTCAGACTCTTCAGGTAGGTGACCTGTTTGTTTTTCCAAAGGGACTTGTTCACTACCAGTACAATGCCGATTCACAAAACTCGGCTACAGCAATTTCTGCTTTTGGAAGTGCAAGTGCAGGAACTGTGTCAATTTCTTCCACTTTGTTCGCCACCAACATCGATGACAATGCCTTGGCTTTGTCCTTCAAGACTGATATAGCTACAATTCAACAGCTTAAGGCTGGTCTCTCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

156

Amino Acids

16.74

Weight (kDa)

4.84

Isoelectric Point (pI)

29.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 9 - 143 1.8e-29 Cupin
Cupin_2 PF07883 37 - 106 3e-11 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 182
AccB1I GGYRCC 1 cut(s) 182
AccI GTMKAC 1 cut(s) 204
AciI CCGC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 2 cut(s) 68, 221
AfaI GTAC 3 cut(s) 105, 184, 287
AflII CTTAAG 1 cut(s) 443
AgsI TTSAA 4 cut(s) 17, 110, 415, 437
AluBI AGCT 3 cut(s) 163, 428, 442
AluI AGCT 3 cut(s) 163, 428, 442
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 350
ApoI RAATTY 1 cut(s) 110
Asp718I GGTACC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BcoDI GTCTC 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 312
BfrI CTTAAG 1 cut(s) 443
BmcAI AGTACT 1 cut(s) 105
BmiI GGNNCC 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 283
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 54, 242
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 363
BshNI GGYRCC 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 165
BslFI GGGAC 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 165
BspACI CCGC 1 cut(s) 60
BspCNI CTCAG 2 cut(s) 55, 241
BspDI ATCGAT 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 182
BspTI CTTAAG 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 458
BsrI ACTGG 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 182, 352
Bst6I CTCTTC 2 cut(s) 173, 240
BstAFI CTTAAG 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 323
BstDEI CTNAG 2 cut(s) 63, 228
BstEII GGTNACC 1 cut(s) 245
BstF5I GGATG 1 cut(s) 145
BstMAI GTCTC 1 cut(s) 458
BstMWI GCNNNNNNNGC 2 cut(s) 59, 323
BstPI GGTNACC 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 312
Bsu15I ATCGAT 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 387
BtsCI GGATG 1 cut(s) 145
CaiI CAGNNNCTG 1 cut(s) 350
ClaI ATCGAT 1 cut(s) 387
Csp6I GTAC 3 cut(s) 104, 183, 286
CviAII CATG 1 cut(s) 468
CviJI RGCY 9 cut(s) 28, 163, 198, 311, 404, 428, 442, 449, 466
CviKI_1 RGCY 9 cut(s) 28, 163, 198, 311, 404, 428, 442, 449, 466
CviQI GTAC 3 cut(s) 104, 183, 286
DdeI CTNAG 2 cut(s) 63, 228
Eam1104I CTCTTC 2 cut(s) 173, 240
EarI CTCTTC 2 cut(s) 173, 240
Ecl136II GAGCTC 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 68, 221
Eco91I GGTNACC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 163
EcoO65I GGTNACC 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 1 cut(s) 471
FaiI YATR 4 cut(s) 97, 99, 425, 469
FaqI GGGAC 1 cut(s) 282
FatI CATG 1 cut(s) 467
FauNDI CATATG 1 cut(s) 97
FblI GTMKAC 1 cut(s) 204
FokI GGATG 1 cut(s) 132
FriOI GRGCYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 471
HincII GTYRAC 2 cut(s) 178, 205
HindII GTYRAC 2 cut(s) 178, 205
HinfI GANTC 2 cut(s) 232, 296
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 3 cut(s) 178, 205, 277
Hpy188I TCNGA 3 cut(s) 48, 87, 231
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 3 cut(s) 178, 205, 277
HpyAV CCTTC 2 cut(s) 43, 421
HpyCH4III ACNGT 2 cut(s) 182, 352
HpyCH4V TGCA 3 cut(s) 101, 338, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 323
HpyF3I CTNAG 2 cut(s) 63, 228
Hsp92II CATG 1 cut(s) 471
KpnI GGTACC 1 cut(s) 186
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 7 cut(s) 84, 105, 224, 263, 296, 330, 435
MaeIII GTNAC 1 cut(s) 245
MboII GAAGA 5 cut(s) 29, 32, 160, 227, 354
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 5 cut(s) 42, 110, 318, 357, 432
MlyI GAGTC 1 cut(s) 226
MnlI CCTC 6 cut(s) 67, 147, 159, 176, 184, 230
MseI TTAA 2 cut(s) 78, 444
MspA1I CMGCKG 1 cut(s) 62
MspCI CTTAAG 1 cut(s) 443
MwoI GCNNNNNNNGC 2 cut(s) 59, 323
NdeI CATATG 1 cut(s) 97
NlaIII CATG 1 cut(s) 471
NlaIV GGNNCC 1 cut(s) 184
NmeAIII GCCGAG 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 296
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 165
PspEI GGTNACC 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 350
RsaI GTAC 3 cut(s) 105, 184, 287
RsaNI GTAC 3 cut(s) 104, 183, 286
SacI GAGCTC 1 cut(s) 165
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 2 cut(s) 78, 444
ScaI AGTACT 1 cut(s) 105
SchI GAGTC 1 cut(s) 226
SduI GDGCHC 1 cut(s) 165
SfcI CTRYAG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 188, 443
SmoI CTYRAG 2 cut(s) 188, 443
Sse9I AATT 5 cut(s) 42, 110, 318, 357, 432
SsiI CCGC 1 cut(s) 60
SstI GAGCTC 1 cut(s) 165
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 182, 352
TaqI TCGA 2 cut(s) 204, 387
TasI AATT 5 cut(s) 42, 110, 318, 357, 432
TatI WGTACW 2 cut(s) 103, 285
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 2 cut(s) 78, 444
Tru9I TTAA 2 cut(s) 78, 444
TseFI GTSAC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 443
XapI RAATTY 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.