RchiOBHm_Chr5g0046811

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000346
Physical Location & Seq
Forward (+)
43063537 .. 43064082
546 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32478

Sequence Viewer

Length: 546 bp
ATGTCTATGAGCATAATTCTAGTAGTGGCGCCTCCTAATGGAACAGTAGATGGAAACTTCTTCACATACACCGGCATGCGTGTTGTCTTTGAAGAAGATGAGCCTAAAAACTTCACAATTCTGAAGGCAACCTCCGCTGAGTTCCCTGCCCTTAATGGTCAGAGTGTTTCATATGCAGTACTTGAATTTCCATCTGGCATTACTAACCCACCTCACACTCATCCTCGCTCTGTTGAGCTCCTCTTCCTTGTTGACGGTACCCTTGAGGTTGGCTTTGTCGACACAAAAAACAACCTCTTTACTCAGACTCTTCAGGTAGGTGACCTGTTTGTTTTTCCAAAGGGACTTGTTCACTACCAGTACAATGCCGATTCACAAAACTCGGCTACAGCAATTTCTGCTTTTGGAAGTGCAAGTGCAGGAACTGTGTCAATTTCTTCCACTTTGTTCGCCACCAACATCGATGACAATGCCTTGGCTTTGTCCTTCAAGACTGATATAGCTACAATTCAACAGCTTAAGGCTGGTCTCTCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

181

Amino Acids

19.36

Weight (kDa)

4.71

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 34 - 168 2.9e-29 Cupin
Cupin_2 PF07883 62 - 131 4.3e-11 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 2 cut(s) 28, 257
AccI GTMKAC 1 cut(s) 279
AciI CCGC 1 cut(s) 135
AcsI RAATTY 1 cut(s) 185
AcuI CTGAAG 2 cut(s) 143, 296
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 3 cut(s) 180, 259, 362
AfiI CCNNNNNNNGG 1 cut(s) 38
AflII CTTAAG 1 cut(s) 518
AgsI TTSAA 4 cut(s) 92, 185, 490, 512
AluBI AGCT 3 cut(s) 238, 503, 517
AluI AGCT 3 cut(s) 238, 503, 517
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 533
AlwNI CAGNNNCTG 1 cut(s) 425
ApoI RAATTY 1 cut(s) 185
Asp718I GGTACC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 332
BanI GGYRCC 2 cut(s) 28, 257
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 240
BccI CCATC 2 cut(s) 44, 199
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BcoDI GTCTC 1 cut(s) 533
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 1 cut(s) 32
BfrI CTTAAG 1 cut(s) 518
BmcAI AGTACT 1 cut(s) 180
BmiI GGNNCC 2 cut(s) 30, 259
BpuEI CTTGAG 1 cut(s) 284
Bsa29I ATCGAT 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 358
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMII CTCAG 2 cut(s) 129, 317
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 1 cut(s) 230
BsgI GTGCAG 1 cut(s) 438
BshNI GGYRCC 2 cut(s) 28, 257
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 38
BsmAI GTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 357
Bso31I GGTCTC 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 240
BspACI CCGC 1 cut(s) 135
BspCNI CTCAG 2 cut(s) 130, 316
BspDI ATCGAT 1 cut(s) 462
BspLI GGNNCC 2 cut(s) 30, 259
BspT107I GGYRCC 2 cut(s) 28, 257
BspTI CTTAAG 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 533
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 474
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 46, 257, 427
Bst6I CTCTTC 2 cut(s) 248, 315
BstACI GRCGYC 1 cut(s) 29
BstAFI CTTAAG 1 cut(s) 518
BstAPI GCANNNNNTGC 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 2 cut(s) 138, 303
BstEII GGTNACC 1 cut(s) 320
BstF5I GGATG 1 cut(s) 220
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstMAI GTCTC 1 cut(s) 533
BstMWI GCNNNNNNNGC 2 cut(s) 134, 398
BstNSI RCATGY 1 cut(s) 79
BstPI GGTNACC 1 cut(s) 320
BstSFI CTRYAG 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 462
BsuTUI ATCGAT 1 cut(s) 462
BtsCI GGATG 1 cut(s) 220
Cac8I GCNNGC 1 cut(s) 77
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 71
ClaI ATCGAT 1 cut(s) 462
Csp6I GTAC 3 cut(s) 179, 258, 361
CviAII CATG 2 cut(s) 76, 543
CviJI RGCY 9 cut(s) 103, 238, 273, 386, 479, 503, 517, 524, 541
CviKI_1 RGCY 9 cut(s) 103, 238, 273, 386, 479, 503, 517, 524, 541
CviQI GTAC 3 cut(s) 179, 258, 361
DdeI CTNAG 2 cut(s) 138, 303
DinI GGCGCC 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 248, 315
EarI CTCTTC 2 cut(s) 248, 315
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 474
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 533
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 2 cut(s) 143, 296
Eco91I GGTNACC 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 238
EcoO65I GGTNACC 1 cut(s) 320
EcoT14I CCWWGG 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 240
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 2 cut(s) 79, 546
FaiI YATR 8 cut(s) 8, 14, 67, 77, 172, 174, 500, 544
FaqI GGGAC 1 cut(s) 357
FatI CATG 2 cut(s) 75, 542
FauNDI CATATG 1 cut(s) 172
FblI GTMKAC 1 cut(s) 279
FokI GGATG 1 cut(s) 207
FriOI GRGCYC 1 cut(s) 240
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 32
HapII CCGG 1 cut(s) 72
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 2 cut(s) 79, 546
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 2 cut(s) 253, 280
HindII GTYRAC 2 cut(s) 253, 280
HinfI GANTC 2 cut(s) 307, 371
HpaII CCGG 1 cut(s) 72
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 3 cut(s) 253, 280, 352
Hpy188I TCNGA 3 cut(s) 123, 162, 306
Hpy188III TCNNGA 1 cut(s) 490
Hpy8I GTNNAC 3 cut(s) 253, 280, 352
HpyAV CCTTC 2 cut(s) 118, 496
HpyCH4III ACNGT 3 cut(s) 46, 257, 427
HpyCH4V TGCA 3 cut(s) 176, 413, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 398
HpyF3I CTNAG 2 cut(s) 138, 303
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 79, 546
HspAI GCGC 1 cut(s) 29
KasI GGCGCC 1 cut(s) 28
KpnI GGTACC 1 cut(s) 261
LmnI GCTCC 1 cut(s) 243
LpnPI CCDG 8 cut(s) 85, 159, 180, 299, 338, 371, 405, 510
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 320
MboII GAAGA 6 cut(s) 52, 104, 107, 235, 302, 429
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 6 cut(s) 15, 117, 185, 393, 432, 507
Mly113I GGCGCC 1 cut(s) 29
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 7 cut(s) 42, 142, 222, 234, 251, 259, 305
MseI TTAA 2 cut(s) 153, 519
MslI CAYNNNNRTG 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 137
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 1 cut(s) 72
MwoI GCNNNNNNNGC 2 cut(s) 134, 398
NarI GGCGCC 1 cut(s) 29
NdeI CATATG 1 cut(s) 172
NlaIII CATG 2 cut(s) 79, 546
NlaIV GGNNCC 2 cut(s) 30, 259
NmeAIII GCCGAG 1 cut(s) 362
NmuCI GTSAC 1 cut(s) 320
NspI RCATGY 1 cut(s) 79
PaeI GCATGC 1 cut(s) 79
PfeI GAWTC 1 cut(s) 371
PleI GAGTC 1 cut(s) 301
PluTI GGCGCC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 301
Psp124BI GAGCTC 1 cut(s) 240
PspEI GGTNACC 1 cut(s) 320
PspN4I GGNNCC 2 cut(s) 30, 259
PstNI CAGNNNCTG 1 cut(s) 425
RsaI GTAC 3 cut(s) 180, 259, 362
RsaNI GTAC 3 cut(s) 179, 258, 361
RseI CAYNNNNRTG 1 cut(s) 74
SacI GAGCTC 1 cut(s) 240
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 153, 519
ScaI AGTACT 1 cut(s) 180
SchI GAGTC 1 cut(s) 301
SduI GDGCHC 1 cut(s) 240
SfcI CTRYAG 1 cut(s) 387
SfoI GGCGCC 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 263, 518
SmoI CTYRAG 2 cut(s) 263, 518
SphI GCATGC 1 cut(s) 79
Sse9I AATT 6 cut(s) 15, 117, 185, 393, 432, 507
SsiI CCGC 1 cut(s) 135
SspDI GGCGCC 1 cut(s) 28
SspMI CTAG 1 cut(s) 20
SstI GAGCTC 1 cut(s) 240
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 3 cut(s) 46, 257, 427
TaqI TCGA 2 cut(s) 279, 462
TasI AATT 6 cut(s) 15, 117, 185, 393, 432, 507
TatI WGTACW 2 cut(s) 178, 360
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 2 cut(s) 153, 519
Tru9I TTAA 2 cut(s) 153, 519
TseFI GTSAC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 1 cut(s) 159
Vha464I CTTAAG 1 cut(s) 518
XapI RAATTY 1 cut(s) 185
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 1 cut(s) 20
ZrmI AGTACT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.