FvH4_3g25310

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000346
Physical Location & Seq
Reverse (-)
18260104 .. 18260661
558 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25310.t1

Sequence Viewer

Length: 558 bp
ATGGCAATAGCTGGAGATCCAGATATTCTTACCGACTTCATGGCGCCTCCAAATGGAACAGTAGATGGAAACTTCTTCACATACACCGGCATGCGTGTTATTCTGCAAGATGAGCCAAAAAACTTCACAGTATTGAAGGCAACATCGGCTGAGTTCCCTGCTCTTAATGGTCAAAGTGTTTCATATGCAGTACTTTTCTTCACACCCGGCACTATCAATCCGCCTCACACTCATCCTCGCTCCGCTGAGCTCCTCTTCCTTGTTGGCGGTACCCTTGAAGTTGGCTTTGTCGACACAAAGAACAACCTCTTTACTCAGACTCTTCAGTCAGGAGACATGTTTGTTTTTCCCAAGGGACTTGTGCACTTCCAATACAATGCTGACTCAGAAAACCCAGCTATAGCAATTTCTGCTTTTGGAAGTGCAAGCGCAGGAACTGTGTCAATTCCCTCCACTTTGTTCGCTACCAACATCGATGACAATATCTTGGCTTTGTCCTTCAAGACTGATGTAGCTACCGTTCAAAAGCTTAAGGCTAGTTTCGCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

186

Amino Acids

19.74

Weight (kDa)

4.81

Isoelectric Point (pI)

27.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 38 - 174 1.2e-33 Cupin
Cupin_2 PF07883 67 - 137 6.5e-11 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 325
Acc65I GGTACC 1 cut(s) 269
AccB1I GGYRCC 2 cut(s) 43, 269
AccI GTMKAC 1 cut(s) 291
AciI CCGC 3 cut(s) 221, 243, 267
AclWI GGATC 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 2 cut(s) 192, 271
AfiI CCNNNNNNNGG 1 cut(s) 53
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 4 cut(s) 136, 278, 502, 524
AluBI AGCT 5 cut(s) 11, 250, 398, 515, 529
AluI AGCT 5 cut(s) 11, 250, 398, 515, 529
Alw21I GWGCWC 2 cut(s) 252, 366
Alw26I GTCTC 1 cut(s) 327
Alw44I GTGCAC 1 cut(s) 362
AlwI GGATC 1 cut(s) 11
AlwNI CAGNNNCTG 1 cut(s) 437
ApaLI GTGCAC 1 cut(s) 362
Asp718I GGTACC 1 cut(s) 269
AspLEI GCGC 2 cut(s) 46, 431
AsuC2I CCSGG 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 43, 269
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 252, 366
BccI CCATC 1 cut(s) 59
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 530
BlpI GCTNAGC 1 cut(s) 246
BmcAI AGTACT 1 cut(s) 192
Bme1390I CCNGG 1 cut(s) 207
BmiI GGNNCC 2 cut(s) 45, 271
BmrFI CCNGG 1 cut(s) 207
BpmI CTGGAG 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 207
Bsa29I ATCGAT 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse118I RCCGGY 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMII CTCAG 4 cut(s) 141, 237, 329, 399
BseRI GAGGAG 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 394
BshNI GGYRCC 2 cut(s) 43, 269
BshVI ATCGAT 1 cut(s) 474
BsiHKAI GWGCWC 2 cut(s) 252, 366
BsiSI CCGG 2 cut(s) 87, 207
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 369
Bsp1286I GDGCHC 2 cut(s) 252, 366
Bsp143I GATC 1 cut(s) 16
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 3 cut(s) 221, 243, 267
BspCNI CTCAG 4 cut(s) 142, 238, 328, 398
BspDI ATCGAT 1 cut(s) 474
BspLI GGNNCC 2 cut(s) 45, 271
BspPI GGATC 1 cut(s) 11
BspT107I GGYRCC 2 cut(s) 43, 269
BspTI CTTAAG 1 cut(s) 530
BsrFI RCCGGY 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 1 cut(s) 16
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 4 cut(s) 61, 130, 439, 520
Bst6I CTCTTC 2 cut(s) 260, 327
BstACI GRCGYC 1 cut(s) 44
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 92, 427
BstDEI CTNAG 4 cut(s) 150, 246, 315, 385
BstF5I GGATG 1 cut(s) 232
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 2 cut(s) 46, 431
BstKTI GATC 1 cut(s) 19
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 4 cut(s) 112, 146, 410, 542
BstNSI RCATGY 2 cut(s) 94, 340
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 366
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 474
BtsCI GGATG 1 cut(s) 232
Cac8I GCNNGC 2 cut(s) 92, 427
CaiI CAGNNNCTG 1 cut(s) 437
CfoI GCGC 2 cut(s) 46, 431
Cfr10I RCCGGY 1 cut(s) 86
ClaI ATCGAT 1 cut(s) 474
Csp6I GTAC 2 cut(s) 191, 270
CviAII CATG 4 cut(s) 40, 91, 337, 555
CviQI GTAC 2 cut(s) 191, 270
DdeI CTNAG 4 cut(s) 150, 246, 315, 385
DinI GGCGCC 1 cut(s) 45
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
DrdI GACNNNNNNGTC 1 cut(s) 325
DseDI GACNNNNNNGTC 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 260, 327
EarI CTCTTC 2 cut(s) 260, 327
EciI GGCGGA 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 252
EgeI GGCGCC 1 cut(s) 45
EheI GGCGCC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 4 cut(s) 43, 94, 340, 558
FaiI YATR 8 cut(s) 41, 82, 92, 184, 186, 338, 401, 556
FaqI GGGAC 1 cut(s) 369
FatI CATG 4 cut(s) 39, 90, 336, 554
FauNDI CATATG 1 cut(s) 184
FblI GTMKAC 1 cut(s) 291
FokI GGATG 1 cut(s) 219
FriOI GRGCYC 1 cut(s) 252
FspBI CTAG 1 cut(s) 537
GlaI GCGC 2 cut(s) 45, 430
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 47
HapII CCGG 2 cut(s) 87, 207
HhaI GCGC 2 cut(s) 46, 431
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 4 cut(s) 43, 94, 340, 558
Hin6I GCGC 2 cut(s) 44, 429
HinP1I GCGC 2 cut(s) 44, 429
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 2 cut(s) 319, 383
HpaII CCGG 2 cut(s) 87, 207
Hpy166II GTNNAC 2 cut(s) 292, 364
Hpy188I TCNGA 2 cut(s) 318, 388
Hpy188III TCNNGA 3 cut(s) 20, 330, 502
Hpy8I GTNNAC 2 cut(s) 292, 364
HpyAV CCTTC 2 cut(s) 130, 508
HpyCH4III ACNGT 4 cut(s) 61, 130, 439, 520
HpyCH4V TGCA 4 cut(s) 106, 188, 364, 425
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 146, 410, 542
HpyF3I CTNAG 4 cut(s) 150, 246, 315, 385
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 4 cut(s) 43, 94, 340, 558
HspAI GCGC 2 cut(s) 44, 429
KasI GGCGCC 1 cut(s) 43
KpnI GGTACC 1 cut(s) 273
Kzo9I GATC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 245, 255, 550
LpnPI CCDG 7 cut(s) 33, 100, 171, 220, 315, 408, 417
MaeI CTAG 1 cut(s) 537
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 4 cut(s) 67, 190, 247, 314
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 252, 366
MluCI AATT 2 cut(s) 405, 444
Mly113I GGCGCC 1 cut(s) 44
MlyI GAGTC 2 cut(s) 313, 377
MnlI CCTC 6 cut(s) 57, 234, 246, 263, 317, 460
MseI TTAA 2 cut(s) 165, 531
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 245
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 2 cut(s) 87, 207
MspR9I CCNGG 1 cut(s) 207
MwoI GCNNNNNNNGC 4 cut(s) 112, 146, 410, 542
NarI GGCGCC 1 cut(s) 44
NciI CCSGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 184
NdeII GATC 1 cut(s) 16
NlaIII CATG 4 cut(s) 43, 94, 340, 558
NlaIV GGNNCC 2 cut(s) 45, 271
NspI RCATGY 2 cut(s) 94, 340
PaeI GCATGC 1 cut(s) 94
PciI ACATGT 1 cut(s) 336
PleI GAGTC 2 cut(s) 313, 377
PluTI GGCGCC 1 cut(s) 47
PpsI GAGTC 2 cut(s) 313, 377
PscI ACATGT 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 45, 271
PsrI GAACNNNNNNTAC 2 cut(s) 504, 536
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 2 cut(s) 192, 271
RsaNI GTAC 2 cut(s) 191, 270
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 252
SalI GTCGAC 1 cut(s) 290
SaqAI TTAA 2 cut(s) 165, 531
Sau3AI GATC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 192
SchI GAGTC 2 cut(s) 313, 377
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 2 cut(s) 252, 366
SetI ASST 6 cut(s) 13, 252, 309, 400, 517, 531
SfcI CTRYAG 1 cut(s) 399
SfoI GGCGCC 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SphI GCATGC 1 cut(s) 94
Sse9I AATT 2 cut(s) 405, 444
SsiI CCGC 3 cut(s) 221, 243, 267
SspDI GGCGCC 1 cut(s) 43
SspMI CTAG 1 cut(s) 537
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 4 cut(s) 61, 130, 439, 520
TaqI TCGA 2 cut(s) 291, 474
TasI AATT 2 cut(s) 405, 444
TatI WGTACW 1 cut(s) 190
Tru1I TTAA 2 cut(s) 165, 531
Tru9I TTAA 2 cut(s) 165, 531
TspDTI ATGAA 2 cut(s) 28, 171
Vha464I CTTAAG 1 cut(s) 530
VneI GTGCAC 1 cut(s) 362
XceI RCATGY 2 cut(s) 94, 340
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 1 cut(s) 537
ZrmI AGTACT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.