Rh5DG331100

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000346
Physical Location & Seq
Forward (+)
43658392 .. 43658946
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG331100.1

Sequence Viewer

Length: 555 bp
ATGGCCGGAGATCCAGATATTCTTAGTGACTTCATTGCGCCTCCAAATGGAACAGTAGATGGAAACTTTTTCACATACACCGGCATGCGTGTTATCTTTGAAGAAGATGATCCCAAAAACTTCACAGTTCTGAAGGCAACCTCCGCTGAGTTCCCTGCTCTTAATGGTCAGAGTGTTTCATATGCAGTTCTTGAATTCCCTTCTGGCACTACTAACCCACCTCACACTCATCCTCGCTCTGCTGAGCTCCTCTTCCTTGTTGGCGGTTCCCTTGAAGTTGGCTTTGTCGACACAAAAAACAACCTCTTTACTCAGACTCTTCAGGTAGGTGACCTGTTTGTTTTTCCCAAGGGACTCGTGCACTTCCAATACAATGCTGACTCAGAAAACCCAGCTATAGCAATTTCTGCTTTTGGAAGTGCAAGTGCAGGAACTGTGTCAATTCCTTCCACTTTGTTCGCCACCAACATCGATGACAATGTCTTGGCTTTGTCCTTCAAGACTGACGTAGCTACCATTCAAAAGCTTAAGGCTGGTCTCGCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

184

Amino Acids

19.55

Weight (kDa)

4.55

Isoelectric Point (pI)

19.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 38 - 173 4.4e-32 Cupin
Cupin_2 PF07883 63 - 136 6e-11 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 288
AciI CCGC 2 cut(s) 144, 264
AclWI GGATC 2 cut(s) 5, 104
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 2 cut(s) 152, 305
AfiI CCNNNNNNNGG 1 cut(s) 47
AflII CTTAAG 1 cut(s) 527
AgsI TTSAA 5 cut(s) 101, 194, 275, 499, 521
AluBI AGCT 4 cut(s) 247, 395, 512, 526
AluI AGCT 4 cut(s) 247, 395, 512, 526
Alw21I GWGCWC 2 cut(s) 249, 363
Alw26I GTCTC 1 cut(s) 542
Alw44I GTGCAC 1 cut(s) 359
AlwI GGATC 2 cut(s) 5, 104
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 359
ApoI RAATTY 1 cut(s) 194
AspLEI GCGC 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 341
BaeGI GKGCMC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 356
Bbv12I GWGCWC 2 cut(s) 249, 363
BccI CCATC 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 396
BfrI CTTAAG 1 cut(s) 527
BlpI GCTNAGC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 268
Bpu1102I GCTNAGC 1 cut(s) 243
Bsa29I ATCGAT 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse118I RCCGGY 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 33
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 4 cut(s) 138, 234, 326, 396
BseRI GAGGAG 1 cut(s) 239
BseSI GKGCMC 1 cut(s) 363
BseYI CCCAGC 1 cut(s) 391
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 249, 363
BsiSI CCGG 2 cut(s) 6, 81
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 542
Bsp1286I GDGCHC 2 cut(s) 249, 363
Bsp143I GATC 2 cut(s) 10, 109
Bsp1720I GCTNAGC 1 cut(s) 243
BspACI CCGC 2 cut(s) 144, 264
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 139, 235, 325, 395
BspDI ATCGAT 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 268
BspPI GGATC 2 cut(s) 5, 104
BspTI CTTAAG 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 2 cut(s) 10, 109
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 356
Bst4CI ACNGT 3 cut(s) 55, 127, 436
Bst6I CTCTTC 2 cut(s) 257, 324
BstAFI CTTAAG 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 407
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 5 cut(s) 23, 147, 243, 312, 382
BstEII GGTNACC 1 cut(s) 329
BstF5I GGATG 1 cut(s) 229
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 13, 112
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 2 cut(s) 10, 109
BstMWI GCNNNNNNNGC 3 cut(s) 143, 407, 539
BstNSI RCATGY 1 cut(s) 88
BstPI GGTNACC 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 471
BtsCI GGATG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 86
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 80
ClaI ATCGAT 1 cut(s) 471
CviAII CATG 2 cut(s) 85, 552
CviJI RGCY 9 cut(s) 5, 247, 282, 395, 488, 512, 526, 533, 550
CviKI_1 RGCY 9 cut(s) 5, 247, 282, 395, 488, 512, 526, 533, 550
DdeI CTNAG 5 cut(s) 23, 147, 243, 312, 382
DpnI GATC 2 cut(s) 12, 111
DpnII GATC 2 cut(s) 10, 109
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 257, 324
EarI CTCTTC 2 cut(s) 257, 324
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 542
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 152, 305
Eco91I GGTNACC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 2 cut(s) 88, 555
FaiI YATR 6 cut(s) 76, 86, 181, 183, 398, 553
FaqI GGGAC 1 cut(s) 366
FatI CATG 2 cut(s) 84, 551
FauNDI CATATG 1 cut(s) 181
FblI GTMKAC 1 cut(s) 288
FokI GGATG 1 cut(s) 216
FriOI GRGCYC 1 cut(s) 249
GlaI GCGC 1 cut(s) 39
GsaI CCCAGC 1 cut(s) 395
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 6, 81
HhaI GCGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 88, 555
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 3 cut(s) 316, 354, 380
HpaII CCGG 2 cut(s) 6, 81
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 2 cut(s) 289, 361
Hpy188I TCNGA 4 cut(s) 132, 171, 315, 385
Hpy188III TCNNGA 3 cut(s) 14, 191, 499
Hpy8I GTNNAC 2 cut(s) 289, 361
HpyAV CCTTC 4 cut(s) 127, 210, 456, 505
HpyCH4III ACNGT 3 cut(s) 55, 127, 436
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 4 cut(s) 185, 361, 422, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 407, 539
HpyF3I CTNAG 5 cut(s) 23, 147, 243, 312, 382
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 2 cut(s) 88, 555
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 10, 109
LmnI GCTCC 2 cut(s) 252, 547
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 2 cut(s) 26, 329
MalI GATC 2 cut(s) 12, 111
MboI GATC 2 cut(s) 10, 109
MboII GAAGA 4 cut(s) 113, 116, 244, 311
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 2 cut(s) 249, 363
MluCI AATT 3 cut(s) 194, 402, 441
MlyI GAGTC 3 cut(s) 310, 348, 374
MnlI CCTC 6 cut(s) 51, 151, 231, 243, 260, 314
MseI TTAA 2 cut(s) 162, 528
MslI CAYNNNNRTG 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 146
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 2 cut(s) 6, 81
MwoI GCNNNNNNNGC 3 cut(s) 143, 407, 539
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 10, 109
NlaIII CATG 2 cut(s) 88, 555
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 2 cut(s) 26, 329
NspI RCATGY 1 cut(s) 88
PaeI GCATGC 1 cut(s) 88
PflFI GACNNNGTC 1 cut(s) 479
PleI GAGTC 3 cut(s) 310, 348, 374
PpsI GAGTC 3 cut(s) 310, 348, 374
Psp124BI GAGCTC 1 cut(s) 249
PspEI GGTNACC 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 10
PsyI GACNNNGTC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 249
SalI GTCGAC 1 cut(s) 287
SaqAI TTAA 2 cut(s) 162, 528
Sau3AI GATC 2 cut(s) 10, 109
SchI GAGTC 3 cut(s) 310, 348, 374
SduI GDGCHC 2 cut(s) 249, 363
SfcI CTRYAG 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
SphI GCATGC 1 cut(s) 88
Sse9I AATT 3 cut(s) 194, 402, 441
SsiI CCGC 2 cut(s) 144, 264
SstI GAGCTC 1 cut(s) 249
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 3 cut(s) 55, 127, 436
TaiI ACGT 1 cut(s) 510
TaqI TCGA 2 cut(s) 288, 471
TasI AATT 3 cut(s) 194, 402, 441
Tru1I TTAA 2 cut(s) 162, 528
Tru9I TTAA 2 cut(s) 162, 528
TseFI GTSAC 2 cut(s) 26, 329
Tsp45I GTSAC 2 cut(s) 26, 329
TspDTI ATGAA 2 cut(s) 22, 168
Tth111I GACNNNGTC 1 cut(s) 479
Vha464I CTTAAG 1 cut(s) 527
VneI GTGCAC 1 cut(s) 359
XapI RAATTY 1 cut(s) 194
XceI RCATGY 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.