FvH4_3g25320

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000346
Physical Location & Seq
Reverse (-)
18272555 .. 18275061
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25320.t1

Sequence Viewer

Length: 543 bp
ATGGCTTCAAGAACTTCCAGCCTCAAATATTTCTCACTACTAGTTCTTTCACTTGCCATTGTTCAAATGACATTGGCAGGAGACCCTGACATTCTTACCGACTTCATTGCCCCTCCAAATGGAACTGTGGACGGAAACTTCTTCACATTCACCGGCATGCGAGTTCTTGTTGGAGGAGGCCCTCCCAAAGCCTTCACCGTGTTGAAGGCAACCTTGACAGAGTTTCCTGCTCTTAATGGGCAGAGTGTTTCATATGCAGTCCTTGAATTCCCATCTGGCACTACTAACCCACCGCACACTCATCCTCGCTCCGCCGAGCTTCTTTTCCTTGTTGAGGGCACCCTTGAAGTTGGCTTTGTTGACACAAAGAATGACCTCTTTACGCAGACTCTTCAATCCGGTGACCTTGCCAATGCAGGAACTGTATCACTTCCTTCAACTTTGTTCGCCACCAACATCGATGACAATGTATTGGCTTTGTCCTTCAAGACTGATGTAGCCACTATTCAGAAGCTCAAGGCCGGTCTTGCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

181

Amino Acids

19.01

Weight (kDa)

4.99

Isoelectric Point (pI)

24.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 63 - 171 1.2e-14 Cupin
Cupin_2 PF07883 87 - 136 3.1e-06 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AciI CCGC 2 cut(s) 293, 312
AcsI RAATTY 1 cut(s) 266
AfiI CCNNNNNNNGG 2 cut(s) 119, 334
AgsI TTSAA 8 cut(s) 9, 65, 205, 266, 347, 395, 438, 487
AhlI ACTAGT 1 cut(s) 40
AluBI AGCT 2 cut(s) 319, 514
AluI AGCT 2 cut(s) 319, 514
Alw26I GTCTC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 2 cut(s) 178, 519
ApoI RAATTY 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 142, 187, 413
BaeGI GKGCMC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 338
BccI CCATC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BcoDI GTCTC 1 cut(s) 75
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 1 cut(s) 340
BpuEI CTTGAG 1 cut(s) 500
Bsa29I ATCGAT 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 334
Bse118I RCCGGY 2 cut(s) 152, 521
Bse3DI GCAATG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 459
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 119, 334
BseMI GCAATG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 189
BseSI GKGCMC 1 cut(s) 341
BshFI GGCC 2 cut(s) 180, 521
BshNI GGYRCC 1 cut(s) 338
BshVI ATCGAT 1 cut(s) 459
BsiSI CCGG 3 cut(s) 153, 399, 522
BslI CCNNNNNNNGG 2 cut(s) 119, 334
BsmAI GTCTC 1 cut(s) 75
BsnI GGCC 2 cut(s) 180, 521
Bso31I GGTCTC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 341
BspACI CCGC 2 cut(s) 293, 312
BspANI GGCC 2 cut(s) 180, 521
BspDI ATCGAT 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 105
BsrFI RCCGGY 2 cut(s) 152, 521
BssAI RCCGGY 2 cut(s) 152, 521
Bst4CI ACNGT 3 cut(s) 127, 199, 424
Bst6I CTCTTC 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 158
BstEII GGTNACC 1 cut(s) 401
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstNSI RCATGY 1 cut(s) 160
BstPI GGTNACC 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 341
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 2 cut(s) 180, 521
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 158
CaiI CAGNNNCTG 1 cut(s) 422
Cfr10I RCCGGY 2 cut(s) 152, 521
Cfr13I GGNCC 1 cut(s) 179
ClaI ATCGAT 1 cut(s) 459
CviAII CATG 2 cut(s) 157, 540
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
EciI GGCGGA 1 cut(s) 301
Eco31I GGTCTC 1 cut(s) 75
Eco91I GGTNACC 1 cut(s) 401
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 266
FaeI CATG 2 cut(s) 160, 543
FaiI YATR 4 cut(s) 158, 253, 255, 541
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 2 cut(s) 156, 539
FauNDI CATATG 1 cut(s) 253
FokI GGATG 1 cut(s) 288
FspBI CTAG 1 cut(s) 41
HaeIII GGCC 2 cut(s) 180, 521
HapII CCGG 3 cut(s) 153, 399, 522
Hin1II CATG 2 cut(s) 160, 543
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 388
HpaII CCGG 3 cut(s) 153, 399, 522
HphI GGTGA 3 cut(s) 142, 187, 413
Hpy166II GTNNAC 2 cut(s) 130, 361
Hpy188I TCNGA 1 cut(s) 510
Hpy188III TCNNGA 2 cut(s) 9, 487
Hpy8I GTNNAC 2 cut(s) 130, 361
HpyAV CCTTC 4 cut(s) 199, 202, 444, 493
HpyCH4III ACNGT 3 cut(s) 127, 199, 424
HpyCH4V TGCA 2 cut(s) 257, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
Hsp92II CATG 2 cut(s) 160, 543
LmnI GCTCC 2 cut(s) 314, 535
LpnPI CCDG 9 cut(s) 31, 63, 99, 166, 240, 261, 402, 412, 535
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 401
MboII GAAGA 2 cut(s) 133, 383
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 1 cut(s) 266
MlyI GAGTC 1 cut(s) 382
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 8 cut(s) 32, 123, 167, 170, 192, 315, 328, 386
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 3 cut(s) 153, 399, 522
MwoI GCNNNNNNNGC 1 cut(s) 527
NdeI CATATG 1 cut(s) 253
NlaIII CATG 2 cut(s) 160, 543
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 401
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PspEI GGTNACC 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 422
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 1 cut(s) 234
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 382
SduI GDGCHC 1 cut(s) 341
SetI ASST 5 cut(s) 215, 321, 378, 408, 516
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
SpeI ACTAGT 1 cut(s) 40
SphI GCATGC 1 cut(s) 160
Sse9I AATT 1 cut(s) 266
SsiI CCGC 2 cut(s) 293, 312
SspI AATATT 1 cut(s) 29
SspMI CTAG 1 cut(s) 41
TaaI ACNGT 3 cut(s) 127, 199, 424
TaqI TCGA 1 cut(s) 459
TasI AATT 1 cut(s) 266
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseFI GTSAC 1 cut(s) 401
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 2 cut(s) 94, 240
TspGWI ACGGA 1 cut(s) 147
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 266
XceI RCATGY 1 cut(s) 160
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.