pycom02g19060

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000346
Physical Location & Seq
Forward (+)
17081760 .. 17086949
5190 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g19060.17

Sequence Viewer

Length: 3579 bp
ATGGCGAAAATCGCTGGAATTTGCTCGGGGTGGGGCGCGGGTGTACAGAAGGGAAAGGGAGGTCTCTTTCCTCTTTTCTTTCTTTCCCCCACCTTTTCCCTCTTAAAATGGCCGGTCCCCTTAATCCCAAAAAGGGTTTGGAGTTTCACAACTTCAAACGTCCGTAACTATACCATTATAATTCGGACTTGCAAATGGCTTTCGCCTATGCGCTCGTGGCTTCGAGACCTATTCGAAAATGTTACTTGTGACCTCAAAAGGTCAATGAATAAAATCTCAATAATAAAAATACATAAATGGGTTATTTCCTCCAATTTTCTGATTTCTCTCTCTAATCTCTCTACTCTGTCCCTCCCTTCCCCTCTCTCTCTAAAATTGATATTTATTGGTTATTTGATTTTGATTATATATGCCTTGTATGAATGCAAGAAAAAAGAAGGTCCCTTTAATGATTTATTTGGAGATTGTTTGTGTTTGTTGTTGGCCCTTTTGGGAAATGAACACATTACTGTGCTTGTAGTTGCAGATTGGGGATTTCTTTCTCAAATCATCATCTTCTTCCCCAAATTCGAAATCCCATTCAAAACCCGTTTGGGTCAAAACCAATCTGAAAAACTAAAAACACCATTTTTTATGCTGCAATCTCTCTCTCTCTCTCTCTCTCTCTCTTCTGCCGATGAATGTCTTCTCCCTCTAAGTTTGAAGCAAATCCCAAATTTGTTATATGTATCAATACGTGCAACGACGTCGGTTTTGTTGTGGACATGCATCATGCAATTAACAGCGTTGGGGGATACGTGGGAGCCTAGATTATGGAAATCCCTTATGGACTATTTTCAAAAACTGAACTCAGGACTAACTCAAAAATACAGTCTATTCTCTAAAAACATAAAAAGTGAGTTTTTAGAGTTTTTAAACGTAAACTCACTCCCTTCTTTTCTCTCACTCCAAATCTATCTCTCTCTTTTCTTCTTTCTCTACTCTTTTTTTTTTTTAACCTTTTTCGTCTCTCCTCCAATCCGCTATCTTCATTCTCTCGGTTACTTCTTTCACTCCTCTTCCTCTCTATCTCGTTCGATCCTCTCTCTTCTTTCTCTTTCCTTCAATCATCTCTTACTTTCTTTCCTTATTTCTCCTCTTTTTCTCTCTCTTTTGAAAATTTTAAATTGCAAACCAATCAAGTTTTTGAATCTTAAAGAAAATTTAAAATTTTCAAATAGGATACTAAACAGGCCCTTAAAGATATGTATACAAATCTTTGGCCTAAACATAGATTCTGGAAATCCCATTAAAAATCATCTCTTTCCAGCTAATAGATCTGAGCCAACCCAAAAACCCAGAGCGCACATCTTTCTCTTCAAAATTAGAGCAATGATGTTGTCACTTGTGACATCCCCACCATATACACTATATACACACCACATGAACATCATATGCACAGTACATGCACAAGATATGCATAGAACTGGAGATCGAGCAACGAGCTACTTTTCTGTTACATATATAAGGTTACAGTGGCAGTTTGAAAAGTCTTGTTACGTATACCAACCATTGCTTAATTGTGCACGTGGAAAACGTGATACTCGTTGTCGTTATTTCATGAACACGCCAACTAGGTTGTGGTCATACTCGCTAAATTTCGGGGCACAACATACATGCATCATAACTAGTGAACATGCCCCACATGCATATTATGGAAAAAAATTCACAAATTTCTACATTGAACAATTTCGAACCATAAATTGTTCCATCCCAGTTTTGTACTTACAAACAACCCATTTATTCCATAAATTATTGATAATCGTCGAAAAAAATGGAACTTTTCGGTTAAAACAGAAAATATGGGATAAACGTCCTATCTTTAAGGATGGAAGAGTTTGTTGTGTTTGGTCACTCTTTTCACCTTTCTTTCGCATCTCTTTGTTCGAAATCAGAAATATGATGTCAACCCAAAACCCAGCTCAGACTTCTCACCTCTCTTTGGACTTCCCTCACTTTGAAATCCGAACTCAGAGATCTGAGCTCAACCCAGAAACCAAGGGCTCACAAAGCTTTCTCAACATCCCTCTTCCAAATGAGAAAATAATTTTGATTTTGTGCATGGTGTGCGTGGCTTGTGGATGGCCAATTTATCCTATTTCTTTCCCAAGATTAAGAAAATGGGTTATTTTTGTCTATTTTCCTAAAAAAAAAAATGAAATTATAACATATAGTTTCAAATGTTGTAAGAAATTGAAAGAGGCGAAGGATAACCCGGGAAATAAGCATGTGAAGGTCTTTGAGGACCCTCTAAATGTTTTTACGGGGACCTTTTATTGCGTAGACAACACTCGACAAATGCTCTCAATAGCACTCATCAGATTGTTTCGAAAAATGCTGATATTCATTGACATATGTGTCTCCATGAATAAACTGAAATTTGTTTACAGCTTCATGCTATGTTTTTTATCTAAAAATCTTGGACCTTTAACATTGGGACTCCCAAGAGATCAAAGCTCTCAAAACCAATTAATTGTAGGACATGAAAACACCATGCTTCTATGTAGACTTTGTAGGGTACATCGCGGCTCCAAATCACCTTATGACCTTGAAGAACCATTTAATAGTAGATCCAACAAACCTCGGGCAAGGTCAGGTGACTCACTGAGTTTCTGGAATAATTACATGCACAAAACTAAAATAAAACAAAATACAAAAAGAATGCTCAATTTTACTCGAGAAGGTCCTCCCATTGATGTCTTAATTTGGTACACCGATGCTTCACTGATGCAAAAGTCCAGGGGCTGTTACTTTCCATCTTGGATATTGTCATTTTTTTTCCTTTTTGGTCAATTGGTGATATTTTTCAAGGATGGAGAAAGTAGAGGCTTAGACTTCATTTTCTTGGGTCAAGTACTGTTAATTGTCTCTCATAGTGTTGGAAAGTTCCTTACATACATAGCTTCGCATCGGTTTAGTATCACTGGCCTACCAGTGTTGCTTAAACGCACAAGCTTGGAGTACACGTCCAAGCAAGATTCGACCAACAATCTAATTTCCCCACTATATAAAAAGCTTCAGAAATTTTCAAAAGAAAAGGGCTCATCAAATCAAATGCGACTTATAATAGTGTCCCTTTTTAGGGGTTTGCTTTCTTTTCCACTGTTTAGGTGTATTTTTATCCTTTTTTATAGAGGGGCTGGCGGGGTTTCGAAAATGTTGGTTCGCTCATTCAGATTTAACGATGGATGCTTTGCACATTATTGGCATGATAATATGACAGTCATTATTAGAGTACTCTATTGTTATTCTCGCTTGGCAAAGGGGCATAATCGAACCAACTTCGGATTGGGGGAGAAGCACTCCTATACTCCCATCTCCATCAACACATTAGGAGAGATATACGAAGGTATATGTAATCAAATTCCCATGACAAAAATTCATAACTTATGGAACGATGGCACTACACACATATACTACTATCGTAATACTCAATCGCATATTCATTGGGCTTATCATTTGCCCTTAAAGGCTGACGAGATAACCACTTTCGGCAAACAAATCATAGACTCTTCGCCGCCTAAGACATGGTATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

1193

Amino Acids

138.51

Weight (kDa)

9.76

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 179, 2204, 3104
AasI GACNNNNNNGTC 1 cut(s) 2396
AatII GACGTC 1 cut(s) 749
AccI GTMKAC 4 cut(s) 1249, 1542, 2322, 2545
AccII CGCG 2 cut(s) 38, 2565
AciI CCGC 5 cut(s) 38, 1021, 2565, 3183, 3557
AclWI GGATC 2 cut(s) 1072, 2604
AcoI YGGCCR 2 cut(s) 110, 2123
AcuI CTGAAG 1 cut(s) 3041
AcvI CACGTG 1 cut(s) 1568
AcyI GRCGYC 1 cut(s) 746
AfaI GTAC 8 cut(s) 45, 1443, 1763, 2559, 2750, 2895, 3002, 3276
AfiI CCNNNNNNNGG 4 cut(s) 133, 1981, 3120, 3121
AflIII ACRYGT 1 cut(s) 3003
AhlI ACTAGT 1 cut(s) 1667
AjiI CACGTC 1 cut(s) 3006
AjnI CCWGG 1 cut(s) 2777
Alw21I GWGCWC 2 cut(s) 1567, 2025
Alw26I GTCTC 5 cut(s) 68, 219, 1012, 2404, 2911
Alw44I GTGCAC 1 cut(s) 1563
AlwI GGATC 2 cut(s) 1072, 2604
AlwNI CAGNNNCTG 1 cut(s) 2784
Ama87I CYCGRG 4 cut(s) 25, 2254, 2622, 2715
AoxI GGCC 6 cut(s) 110, 483, 1232, 1261, 2123, 2965
ApaLI GTGCAC 1 cut(s) 1563
ApeKI GCWGC 1 cut(s) 637
ArsI GACNNNNNNTTYG 4 cut(s) 736, 768, 856, 888
AseI ATTAAT 1 cut(s) 2510
Asp700I GAANNNNTTC 2 cut(s) 684, 1728
AspLEI GCGC 3 cut(s) 38, 213, 1345
AspS9I GGNCC 8 cut(s) 115, 440, 484, 1233, 2284, 2307, 2462, 2723
AsuC2I CCSGG 2 cut(s) 2255, 2256
AsuHPI GGTGA 5 cut(s) 1893, 1964, 2568, 2648, 2848
AsuII TTCGAA 6 cut(s) 234, 570, 1732, 1926, 2368, 3191
AvaI CYCGRG 4 cut(s) 25, 2254, 2622, 2715
AvaII GGWCC 6 cut(s) 115, 440, 2284, 2307, 2462, 2723
BaeGI GKGCMC 2 cut(s) 1567, 1648
BalI TGGCCA 1 cut(s) 2125
BanII GRGCYC 3 cut(s) 2025, 2045, 3083
BauI CACGAG 1 cut(s) 214
BbrPI CACGTG 1 cut(s) 1568
BbsI GAAGAC 1 cut(s) 677
Bbv12I GWGCWC 2 cut(s) 1567, 2025
BbvI GCAGC 1 cut(s) 624
BccI CCATC 9 cut(s) 1757, 1862, 2115, 2803, 2846, 3218, 3362, 3368, 3431
BcgI CGANNNNNNTGC 2 cut(s) 729, 763
BciT130I CCWGG 1 cut(s) 2779
BciVI GTATCC 2 cut(s) 787, 1215
BcnI CCSGG 2 cut(s) 2255, 2256
BcoDI GTCTC 5 cut(s) 68, 219, 1012, 2404, 2911
BcuI ACTAGT 1 cut(s) 1667
BfaI CTAG 3 cut(s) 807, 1614, 1668
BfuI GTATCC 2 cut(s) 787, 1215
BglII AGATCT 2 cut(s) 1316, 2015
BisI GCNGC 3 cut(s) 638, 2566, 3557
BlsI GCNGC 3 cut(s) 639, 2567, 3558
BmcAI AGTACT 2 cut(s) 2895, 3276
Bme1390I CCNGG 3 cut(s) 2255, 2256, 2779
Bme18I GGWCC 6 cut(s) 115, 440, 2284, 2307, 2462, 2723
BmeT110I CYCGRG 4 cut(s) 25, 2254, 2622, 2715
BmgBI CACGTC 1 cut(s) 3006
BmgT120I GGNCC 8 cut(s) 115, 440, 484, 1233, 2284, 2307, 2462, 2723
BmiI GGNNCC 6 cut(s) 117, 442, 804, 2286, 2308, 2569
BmrFI CCNGG 3 cut(s) 2255, 2256, 2779
BmrI ACTGGG 1 cut(s) 1748
BmsI GCATC 7 cut(s) 777, 1668, 1923, 2746, 2757, 2956, 3218
BmuI ACTGGG 1 cut(s) 1748
BpiI GAAGAC 1 cut(s) 677
BplI GAGNNNNNCTC 2 cut(s) 3326, 3358
BpmI CTGGAG 1 cut(s) 1488
Bpu14I TTCGAA 6 cut(s) 234, 570, 1732, 1926, 2368, 3191
BpuMI CCSGG 2 cut(s) 2255, 2256
BsaAI YACGTR 4 cut(s) 737, 798, 1540, 1568
BsaHI GRCGYC 1 cut(s) 746
BsaI GGTCTC 2 cut(s) 68, 219
BsaJI CCNNGG 4 cut(s) 2037, 2254, 2621, 2778
BsaXI ACNNNNNCTCC 2 cut(s) 2990, 3020
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 1981, 3120, 3121
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 4 cut(s) 1471, 1754, 2968, 2972
Bse3DI GCAATG 2 cut(s) 1377, 1550
BseBI CCWGG 1 cut(s) 2779
BseDI CCNNGG 4 cut(s) 2037, 2254, 2621, 2778
BseGI GGATG 7 cut(s) 1391, 1749, 1873, 2061, 2126, 2857, 3233
BseLI CCNNNNNNNGG 4 cut(s) 133, 1981, 3120, 3121
BseMI GCAATG 2 cut(s) 1377, 1550
BseMII CTCAG 6 cut(s) 864, 1311, 1976, 2010, 2024, 2636
BseNI ACTGG 4 cut(s) 1471, 1754, 2968, 2972
BseRI GAGGAG 3 cut(s) 1002, 1045, 1125
BseSI GKGCMC 2 cut(s) 1567, 1648
BseXI GCAGC 1 cut(s) 624
BseYI CCCAGC 1 cut(s) 1957
Bsh1236I CGCG 2 cut(s) 38, 2565
BshFI GGCC 6 cut(s) 112, 485, 1234, 1263, 2125, 2967
BsiHKAI GWGCWC 2 cut(s) 1567, 2025
BsiHKCI CYCGRG 4 cut(s) 25, 2254, 2622, 2715
BsiSI CCGG 2 cut(s) 113, 2255
BslFI GGGAC 6 cut(s) 101, 334, 426, 2320, 2490, 3097
BslI CCNNNNNNNGG 4 cut(s) 133, 1981, 3120, 3121
BsmAI GTCTC 5 cut(s) 68, 219, 1012, 2404, 2911
BsmBI CGTCTC 1 cut(s) 1012
BsmFI GGGAC 6 cut(s) 101, 334, 426, 2320, 2490, 3097
BsmI GAATGC 2 cut(s) 428, 2706
BsnI GGCC 6 cut(s) 112, 485, 1234, 1263, 2125, 2967
Bso31I GGTCTC 2 cut(s) 68, 219
BsoBI CYCGRG 4 cut(s) 25, 2254, 2622, 2715
Bsp119I TTCGAA 6 cut(s) 234, 570, 1732, 1926, 2368, 3191
Bsp1286I GDGCHC 5 cut(s) 1567, 1648, 2025, 2045, 3083
Bsp1407I TGTACA 1 cut(s) 43
Bsp143I GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
BspACI CCGC 5 cut(s) 38, 1021, 2565, 3183, 3557
BspANI GGCC 6 cut(s) 112, 485, 1234, 1263, 2125, 2967
BspCNI CTCAG 6 cut(s) 863, 1312, 1975, 2011, 2023, 2637
BspFNI CGCG 2 cut(s) 38, 2565
BspHI TCATGA 1 cut(s) 1599
BspLI GGNNCC 6 cut(s) 117, 442, 804, 2286, 2308, 2569
BspPI GGATC 2 cut(s) 1072, 2604
BspT104I TTCGAA 6 cut(s) 234, 570, 1732, 1926, 2368, 3191
BspTNI GGTCTC 2 cut(s) 68, 219
BsrDI GCAATG 2 cut(s) 1377, 1550
BsrFI RCCGGY 1 cut(s) 112
BsrGI TGTACA 1 cut(s) 43
BsrI ACTGG 4 cut(s) 1471, 1754, 2968, 2972
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 4 cut(s) 2037, 2254, 2621, 2778
BssMI GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
BssNAI GTATAC 2 cut(s) 1250, 1543
BssNI GRCGYC 1 cut(s) 746
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 2037
Bst1107I GTATAC 2 cut(s) 1250, 1543
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 2779
Bst4CI ACNGT 7 cut(s) 511, 872, 1441, 1515, 2898, 3144, 3262
Bst6I CTCTTC 7 cut(s) 673, 1063, 1092, 1361, 1867, 2073, 3556
BstACI GRCGYC 1 cut(s) 746
BstAPI GCANNNNNTGC 1 cut(s) 2106
BstAUI TGTACA 1 cut(s) 43
BstBAI YACGTR 4 cut(s) 737, 798, 1540, 1568
BstBI TTCGAA 6 cut(s) 234, 570, 1732, 1926, 2368, 3191
BstC8I GCNNGC 1 cut(s) 3181
BstDEI CTNAG 9 cut(s) 695, 850, 1320, 1962, 2010, 2019, 2645, 2869, 3561
BstF5I GGATG 7 cut(s) 1391, 1749, 1873, 2061, 2126, 2857, 3233
BstFNI CGCG 2 cut(s) 38, 2565
BstHHI GCGC 3 cut(s) 38, 213, 1345
BstKTI GATC 6 cut(s) 1080, 1319, 1474, 2018, 2491, 2612
BstMAI GTCTC 5 cut(s) 68, 219, 1012, 2404, 2911
BstMBI GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
BstMWI GCNNNNNNNGC 5 cut(s) 11, 217, 1685, 2049, 2106
BstNI CCWGG 1 cut(s) 2779
BstNSI RCATGY 7 cut(s) 768, 1448, 1659, 1679, 1688, 2270, 2668
BstSCI CCNGG 3 cut(s) 2253, 2254, 2777
BstSLI GKGCMC 2 cut(s) 1567, 1648
BstSNI TACGTA 1 cut(s) 1540
BstUI CGCG 2 cut(s) 38, 2565
BstV1I GCAGC 1 cut(s) 624
BstV2I GAAGAC 1 cut(s) 677
BstX2I RGATCY 3 cut(s) 1316, 2015, 2609
BstYI RGATCY 3 cut(s) 1316, 2015, 2609
BstZ17I GTATAC 2 cut(s) 1250, 1543
BsuI GTATCC 2 cut(s) 787, 1215
BsuRI GGCC 6 cut(s) 112, 485, 1234, 1263, 2125, 2967
BtgZI GCGATG 1 cut(s) 2546
BtrI CACGTC 1 cut(s) 3006
BtsCI GGATG 7 cut(s) 1391, 1749, 1873, 2061, 2126, 2857, 3233
BtsIMutI CAGTG 6 cut(s) 1520, 2642, 2762, 2961, 2979, 3140
Cac8I GCNNGC 1 cut(s) 3181
CaiI CAGNNNCTG 1 cut(s) 2784
CciI TCATGA 1 cut(s) 1599
CfoI GCGC 3 cut(s) 38, 213, 1345
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 8 cut(s) 115, 440, 484, 1233, 2284, 2307, 2462, 2723
Cfr9I CCCGGG 1 cut(s) 2254
Csp6I GTAC 8 cut(s) 44, 1442, 1762, 2558, 2749, 2894, 3001, 3275
CviQI GTAC 8 cut(s) 44, 1442, 1762, 2558, 2749, 2894, 3001, 3275
DdeI CTNAG 9 cut(s) 695, 850, 1320, 1962, 2010, 2019, 2645, 2869, 3561
DpnI GATC 6 cut(s) 1079, 1318, 1473, 2017, 2490, 2611
DpnII GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
DraI TTTAAA 3 cut(s) 915, 1164, 1206
DrdI GACNNNNNNGTC 1 cut(s) 2396
DseDI GACNNNNNNGTC 1 cut(s) 2396
EaeI YGGCCR 2 cut(s) 110, 2123
Eam1104I CTCTTC 7 cut(s) 673, 1063, 1092, 1361, 1867, 2073, 3556
EarI CTCTTC 7 cut(s) 673, 1063, 1092, 1361, 1867, 2073, 3556
Ecl136II GAGCTC 1 cut(s) 2023
Eco105I TACGTA 1 cut(s) 1540
Eco130I CCWWGG 1 cut(s) 2037
Eco24I GRGCYC 3 cut(s) 2025, 2045, 3083
Eco31I GGTCTC 2 cut(s) 68, 219
Eco47I GGWCC 6 cut(s) 115, 440, 2284, 2307, 2462, 2723
Eco53kI GAGCTC 1 cut(s) 2023
Eco57I CTGAAG 1 cut(s) 3041
Eco72I CACGTG 1 cut(s) 1568
Eco88I CYCGRG 4 cut(s) 25, 2254, 2622, 2715
EcoICRI GAGCTC 1 cut(s) 2023
EcoO109I RGGNCCY 5 cut(s) 440, 1233, 2284, 2307, 2723
EcoRII CCWGG 1 cut(s) 2777
EcoT14I CCWWGG 1 cut(s) 2037
EcoT22I ATGCAT 4 cut(s) 770, 1461, 1661, 1690
EcoT38I GRGCYC 3 cut(s) 2025, 2045, 3083
ErhI CCWWGG 1 cut(s) 2037
Esp3I CGTCTC 1 cut(s) 1012
FaqI GGGAC 6 cut(s) 101, 334, 426, 2320, 2490, 3097
FauI CCCGC 2 cut(s) 31, 3176
FauNDI CATATG 2 cut(s) 1433, 2393
FblI GTMKAC 4 cut(s) 1249, 1542, 2322, 2545
Fnu4HI GCNGC 3 cut(s) 638, 2566, 3557
FokI GGATG 7 cut(s) 1378, 1736, 1880, 2048, 2133, 2864, 3240
FriOI GRGCYC 3 cut(s) 2025, 2045, 3083
Fsp4HI GCNGC 3 cut(s) 638, 2566, 3557
FspBI CTAG 3 cut(s) 807, 1614, 1668
GlaI GCGC 3 cut(s) 37, 212, 1344
GluI GCNGC 3 cut(s) 638, 2566, 3557
GsaI CCCAGC 1 cut(s) 1961
GsuI CTGGAG 1 cut(s) 1488
HaeIII GGCC 6 cut(s) 112, 485, 1234, 1263, 2125, 2967
HapII CCGG 2 cut(s) 113, 2255
HhaI GCGC 3 cut(s) 38, 213, 1345
Hin1I GRCGYC 1 cut(s) 746
Hin6I GCGC 3 cut(s) 36, 211, 1343
HinP1I GCGC 3 cut(s) 36, 211, 1343
HincII GTYRAC 1 cut(s) 1947
HindII GTYRAC 1 cut(s) 1947
HindIII AAGCTT 3 cut(s) 2050, 2992, 3053
HinfI GANTC 6 cut(s) 1189, 1274, 2478, 2639, 3017, 3548
HpaII CCGG 2 cut(s) 113, 2255
HphI GGTGA 5 cut(s) 1893, 1964, 2568, 2648, 2848
Hpy188III TCNNGA 6 cut(s) 224, 852, 1278, 1600, 2653, 2717
Hpy99I CGWCG 3 cut(s) 748, 751, 1808
HpyAV CCTTC 9 cut(s) 43, 366, 431, 942, 1111, 2239, 2266, 2714, 3380
HpyCH4III ACNGT 7 cut(s) 511, 872, 1441, 1515, 2898, 3144, 3262
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 217, 1685, 2049, 2106
HpyF3I CTNAG 9 cut(s) 695, 850, 1320, 1962, 2010, 2019, 2645, 2869, 3561
Hsp92I GRCGYC 1 cut(s) 746
HspAI GCGC 3 cut(s) 36, 211, 1343
Kzo9I GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
LmnI GCTCC 2 cut(s) 802, 2573
Lsp1109I GCAGC 1 cut(s) 624
LweI GCATC 7 cut(s) 777, 1668, 1923, 2746, 2757, 2956, 3218
MaeI CTAG 3 cut(s) 807, 1614, 1668
MalI GATC 6 cut(s) 1079, 1318, 1473, 2017, 2490, 2611
MboI GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
MfeI CAATTG 1 cut(s) 2831
MflI RGATCY 3 cut(s) 1316, 2015, 2609
MhlI GDGCHC 5 cut(s) 1567, 1648, 2025, 2045, 3083
MlsI TGGCCA 1 cut(s) 2125
MluNI TGGCCA 1 cut(s) 2125
MlyI GAGTC 3 cut(s) 2472, 2633, 3542
MmeI TCCRAC 2 cut(s) 2637, 2899
Mox20I TGGCCA 1 cut(s) 2125
Mph1103I ATGCAT 4 cut(s) 770, 1461, 1661, 1690
MroXI GAANNNNTTC 2 cut(s) 684, 1728
MscI TGGCCA 1 cut(s) 2125
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 2125
MspI CCGG 2 cut(s) 113, 2255
MspR9I CCNGG 3 cut(s) 2255, 2256, 2779
MunI CAATTG 1 cut(s) 2831
Mva1269I GAATGC 2 cut(s) 428, 2706
MvaI CCWGG 1 cut(s) 2779
MvnI CGCG 2 cut(s) 38, 2565
MwoI GCNNNNNNNGC 5 cut(s) 11, 217, 1685, 2049, 2106
NciI CCSGG 2 cut(s) 2255, 2256
NdeI CATATG 2 cut(s) 1433, 2393
NdeII GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
NlaIV GGNNCC 6 cut(s) 117, 442, 804, 2286, 2308, 2569
NmuCI GTSAC 5 cut(s) 248, 1380, 1387, 1890, 2636
NsiI ATGCAT 4 cut(s) 770, 1461, 1661, 1690
NspI RCATGY 7 cut(s) 768, 1448, 1659, 1679, 1688, 2270, 2668
NspV TTCGAA 6 cut(s) 234, 570, 1732, 1926, 2368, 3191
PaeR7I CTCGAG 1 cut(s) 2715
PagI TCATGA 1 cut(s) 1599
PcsI WCGNNNNNNNCGW 2 cut(s) 1573, 1582
PctI GAATGC 2 cut(s) 428, 2706
PdmI GAANNNNTTC 2 cut(s) 684, 1728
PfeI GAWTC 3 cut(s) 1189, 1274, 3017
PkrI GCNGC 3 cut(s) 639, 2567, 3558
PleI GAGTC 3 cut(s) 2472, 2633, 3542
PmaCI CACGTG 1 cut(s) 1568
PmlI CACGTG 1 cut(s) 1568
PpsI GAGTC 3 cut(s) 2472, 2633, 3542
Ppu21I YACGTR 4 cut(s) 737, 798, 1540, 1568
PpuMI RGGWCCY 4 cut(s) 440, 2284, 2307, 2723
PshBI ATTAAT 1 cut(s) 2510
PsiI TTATAA 3 cut(s) 179, 2204, 3104
Psp124BI GAGCTC 1 cut(s) 2025
Psp5II RGGWCCY 4 cut(s) 440, 2284, 2307, 2723
Psp6I CCWGG 1 cut(s) 2777
PspCI CACGTG 1 cut(s) 1568
PspFI CCCAGC 1 cut(s) 1957
PspGI CCWGG 1 cut(s) 2777
PspN4I GGNNCC 6 cut(s) 117, 442, 804, 2286, 2308, 2569
PspPI GGNCC 8 cut(s) 115, 440, 484, 1233, 2284, 2307, 2462, 2723
PspPPI RGGWCCY 4 cut(s) 440, 2284, 2307, 2723
PstNI CAGNNNCTG 1 cut(s) 2784
PsuI RGATCY 3 cut(s) 1316, 2015, 2609
RsaI GTAC 8 cut(s) 45, 1443, 1763, 2559, 2750, 2895, 3002, 3276
RsaNI GTAC 8 cut(s) 44, 1442, 1762, 2558, 2749, 2894, 3001, 3275
RseI CAYNNNNRTG 1 cut(s) 509
SacI GAGCTC 1 cut(s) 2025
SatI GCNGC 3 cut(s) 638, 2566, 3557
Sau3AI GATC 6 cut(s) 1077, 1316, 1471, 2015, 2488, 2609
Sau96I GGNCC 8 cut(s) 115, 440, 484, 1233, 2284, 2307, 2462, 2723
ScaI AGTACT 2 cut(s) 2895, 3276
SchI GAGTC 3 cut(s) 2472, 2633, 3542
ScrFI CCNGG 3 cut(s) 2255, 2256, 2779
SduI GDGCHC 5 cut(s) 1567, 1648, 2025, 2045, 3083
SfaNI GCATC 7 cut(s) 777, 1668, 1923, 2746, 2757, 2956, 3218
Sfr274I CTCGAG 1 cut(s) 2715
SfuI TTCGAA 6 cut(s) 234, 570, 1732, 1926, 2368, 3191
SinI GGWCC 6 cut(s) 115, 440, 2284, 2307, 2462, 2723
SlaI CTCGAG 1 cut(s) 2715
SmaI CCCGGG 1 cut(s) 2256
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 2715
SmoI CTYRAG 1 cut(s) 2715
SnaBI TACGTA 1 cut(s) 1540
SpeI ACTAGT 1 cut(s) 1667
SsiI CCGC 5 cut(s) 38, 1021, 2565, 3183, 3557
SspMI CTAG 3 cut(s) 807, 1614, 1668
SstI GAGCTC 1 cut(s) 2025
StyD4I CCNGG 3 cut(s) 2253, 2254, 2777
StyI CCWWGG 1 cut(s) 2037
TaaI ACNGT 7 cut(s) 511, 872, 1441, 1515, 2898, 3144, 3262
TatI WGTACW 6 cut(s) 43, 1441, 1761, 2893, 3000, 3274
TauI GCSGC 2 cut(s) 2568, 3559
TfiI GAWTC 3 cut(s) 1189, 1274, 3017
TscAI CASTG 6 cut(s) 1520, 2649, 2769, 2968, 2979, 3147
TseFI GTSAC 5 cut(s) 248, 1380, 1387, 1890, 2636
TseI GCWGC 1 cut(s) 637
Tsp45I GTSAC 5 cut(s) 248, 1380, 1387, 1890, 2636
TspGWI ACGGA 1 cut(s) 152
TspMI CCCGGG 1 cut(s) 2254
TspRI CASTG 6 cut(s) 1520, 2649, 2769, 2968, 2979, 3147
VneI GTGCAC 1 cut(s) 1563
VpaK11BI GGWCC 6 cut(s) 115, 440, 2284, 2307, 2462, 2723
VspI ATTAAT 1 cut(s) 2510
XceI RCATGY 7 cut(s) 768, 1448, 1659, 1679, 1688, 2270, 2668
XcmI CCANNNNNNNNNTGG 3 cut(s) 135, 1617, 3325
XhoI CTCGAG 1 cut(s) 2715
XmaI CCCGGG 1 cut(s) 2254
XmiI GTMKAC 4 cut(s) 1249, 1542, 2322, 2545
XmnI GAANNNNTTC 2 cut(s) 684, 1728
XspI CTAG 3 cut(s) 807, 1614, 1668
ZraI GACGTC 1 cut(s) 747
ZrmI AGTACT 2 cut(s) 2895, 3276
Zsp2I ATGCAT 4 cut(s) 770, 1461, 1661, 1690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.