MD03G1148000.v1.1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000346
Physical Location & Seq
Forward (+)
16355348 .. 16355971
624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1148000.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGCCTCAAGAACTTATACCCTCAAATTCTTGTCACTACTAATCTTTTCATTTGCCATTGTTCAAATGGCAATGGCTGGAGATCCAGACATTATTTCTGACTTCATAGCGCCCCCAAATGGAACCGTAGACGGAAACTTCTTCACATACACTGGCTTTCGTGTTGTCGGAGGAGGCCCTCCCGCAGACTTCAAGATACTAAAGGCAAGCATGGCTCAGTTCCCTGCTCTCAATGGGCAGAGTGTTTCCTATGCCGTCATTGAATTCCCATCCGGCACTACTAACCCACTACACACTCATCCTCGCTCTGCTGAACTCCTTTTCCTTGCTTATGGTACCCTTGAGGTCGGTTTCGTTGACACCAAAAACAACCTCTTTAACCAAACACTTAAAGCGGGTGATTTGTTTGTGTTTCCAAAGGGACTTATGCACTTCCAGTACAATGCTGATTCACAAAAACCAGCTCTTGCAATTTCTGCCTTAGGAAGTGCAAATGCAGGAACTGTATCAATCCCCTCCACCTTGTTCACAACCGGCATCGGCGACAATGTCTTGGCTATCTCCTTCAAGACTGATGTGGCCACCATTCAAAAGCTCAAGGCCAGTCTTGCCACCAGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

208

Amino Acids

22.04

Weight (kDa)

7.95

Isoelectric Point (pI)

18.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 71 - 196 4.8e-29 Cupin
Cupin_2 PF07883 86 - 158 2.4e-09 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 129
AciI CCGC 2 cut(s) 183, 395
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 579
AcsI RAATTY 2 cut(s) 26, 263
AfaI GTAC 2 cut(s) 337, 440
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 5 cut(s) 65, 193, 263, 568, 590
AjnI CCWGG 1 cut(s) 614
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
AluBI AGCT 2 cut(s) 464, 595
AluI AGCT 2 cut(s) 464, 595
AlwI GGATC 1 cut(s) 77
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 5 cut(s) 3, 175, 579, 600, 617
ApoI RAATTY 2 cut(s) 26, 263
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 481
BalI TGGCCA 1 cut(s) 581
BanI GGYRCC 1 cut(s) 335
BccI CCATC 1 cut(s) 277
BceAI ACGGC 1 cut(s) 239
BciT130I CCWGG 1 cut(s) 616
BfoI RGCGCY 1 cut(s) 113
Bme1390I CCNGG 1 cut(s) 616
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 124, 337
BmrFI CCNGG 1 cut(s) 616
BmsI GCATC 1 cut(s) 546
BpmI CTGGAG 1 cut(s) 99
BpuEI CTTGAG 2 cut(s) 362, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 3 cut(s) 157, 436, 603
Bse21I CCTNAGG 1 cut(s) 481
Bse3DI GCAATG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 616
BseGI GGATG 2 cut(s) 269, 298
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 3 cut(s) 157, 436, 603
BseRI GAGGAG 1 cut(s) 186
BshFI GGCC 5 cut(s) 5, 177, 581, 602, 619
BshNI GGYRCC 1 cut(s) 335
BsiSI CCGG 2 cut(s) 273, 534
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 435
BsnI GGCC 5 cut(s) 5, 177, 581, 602, 619
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 2 cut(s) 183, 395
BspANI GGCC 5 cut(s) 5, 177, 581, 602, 619
BspCNI CTCAG 1 cut(s) 229
BspLI GGNNCC 2 cut(s) 124, 337
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 3 cut(s) 157, 436, 603
BssAI RCCGGY 1 cut(s) 533
BssMI GATC 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 616
Bst4CI ACNGT 2 cut(s) 127, 505
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 216, 481
BstF5I GGATG 2 cut(s) 269, 298
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 212, 476, 608
BstNI CCWGG 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 614
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
Bsu36I CCTNAGG 1 cut(s) 481
BsuRI GGCC 5 cut(s) 5, 177, 581, 602, 619
BtsCI GGATG 2 cut(s) 269, 298
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 208
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 336, 439
CviAII CATG 2 cut(s) 211, 621
CviQI GTAC 2 cut(s) 336, 439
DdeI CTNAG 2 cut(s) 216, 481
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
EaeI YGGCCR 1 cut(s) 579
Eco81I CCTNAGG 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 614
FaeI CATG 2 cut(s) 214, 624
FaiI YATR 8 cut(s) 18, 107, 148, 212, 252, 333, 428, 622
FaqI GGGAC 1 cut(s) 435
FatI CATG 2 cut(s) 210, 620
FauI CCCGC 2 cut(s) 190, 388
FblI GTMKAC 1 cut(s) 129
FokI GGATG 2 cut(s) 256, 285
GlaI GCGC 1 cut(s) 111
GsuI CTGGAG 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 5 cut(s) 5, 177, 581, 602, 619
HapII CCGG 2 cut(s) 273, 534
HhaI GCGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 214, 624
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 1 cut(s) 449
HpaII CCGG 2 cut(s) 273, 534
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 3 cut(s) 130, 358, 528
Hpy188I TCNGA 2 cut(s) 100, 170
Hpy188III TCNNGA 4 cut(s) 9, 86, 193, 568
Hpy8I GTNNAC 3 cut(s) 130, 358, 528
HpyAV CCTTC 1 cut(s) 574
HpyCH4III ACNGT 2 cut(s) 127, 505
HpyCH4V TGCA 4 cut(s) 430, 470, 491, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 476, 608
HpyF3I CTNAG 2 cut(s) 216, 481
Hsp92II CATG 2 cut(s) 214, 624
HspAI GCGC 1 cut(s) 110
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 1 cut(s) 82
LweI GCATC 1 cut(s) 546
MaeIII GTNAC 1 cut(s) 33
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 133
MflI RGATCY 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 3 cut(s) 26, 263, 471
MluNI TGGCCA 1 cut(s) 581
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 9 cut(s) 16, 32, 164, 167, 189, 312, 337, 383, 526
Mox20I TGGCCA 1 cut(s) 581
MscI TGGCCA 1 cut(s) 581
MseI TTAA 2 cut(s) 378, 390
Msp20I TGGCCA 1 cut(s) 581
MspI CCGG 2 cut(s) 273, 534
MspR9I CCNGG 1 cut(s) 616
MvaI CCWGG 1 cut(s) 616
MwoI GCNNNNNNNGC 3 cut(s) 212, 476, 608
NdeII GATC 1 cut(s) 82
NlaIII CATG 2 cut(s) 214, 624
NlaIV GGNNCC 2 cut(s) 124, 337
NmuCI GTSAC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 449
PflFI GACNNNGTC 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 614
PspGI CCWGG 1 cut(s) 614
PspN4I GGNNCC 2 cut(s) 124, 337
PspPI GGNCC 1 cut(s) 176
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 1 cut(s) 82
PsyI GACNNNGTC 1 cut(s) 548
RsaI GTAC 2 cut(s) 337, 440
RsaNI GTAC 2 cut(s) 336, 439
SaqAI TTAA 2 cut(s) 378, 390
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 616
SetI ASST 5 cut(s) 348, 375, 466, 524, 597
SfaNI GCATC 1 cut(s) 546
SmlI CTYRAG 3 cut(s) 7, 341, 596
SmoI CTYRAG 3 cut(s) 7, 341, 596
Sse9I AATT 3 cut(s) 26, 263, 471
SsiI CCGC 2 cut(s) 183, 395
StyD4I CCNGG 1 cut(s) 614
TaaI ACNGT 2 cut(s) 127, 505
TasI AATT 3 cut(s) 26, 263, 471
TatI WGTACW 1 cut(s) 438
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 2 cut(s) 378, 390
Tru9I TTAA 2 cut(s) 378, 390
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 33
TspDTI ATGAA 2 cut(s) 39, 94
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 1 cut(s) 157
Tth111I GACNNNGTC 1 cut(s) 548
XapI RAATTY 2 cut(s) 26, 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.