Rw5G029070

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000346
Physical Location & Seq
Forward (+)
44295314 .. 44295940
627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029070.1

Sequence Viewer

Length: 627 bp
ATGGCCTCGAGAACTTCAACCCTAAAATTCTTCTCACTAATAATCTGTTCTCTTGCAATCGCTGAAATGGCAATGGCCGGAGATCCAGATATTCTTAGTGACTTCATTGCGCCCCCAAATGGAACAGTAGATGGAAACTTCTTCACATACACCGGCATGCGTGTTATCTTTCAAGAAGATGATCCCAAAAACTTCACAGTTCTGAAGGCAACCTCCGCTGAGTTCCCTGCTCTTAATGGTCAGAGTGTTTCATATGCTGTACTTGAATTCCCTTCTGGCACTACTAACCCACCTCACACTCATCCTCGCTCTGCTGAGCTCCTCTTCCTTGTTGGCGGTTCCCTTGAAGTTGGCTTTGTCGACACAAAAAACAACCTCTTTACTCAGACTCTTCAGGTAGGTGACCTGTTTGTTTTTCCCAAGGGACTTGTACACTTCCAATACAATGCTGACTCAGAAAGCCCAGCTATAGCAATTTCTGCTTTTGGAAGTGCAAGTGCAGGAACTGTGTCAATTCCTTCCACTTTGTTCGCCACCAACATCGATGACAATGTCTTGGCTTTGTCCTTCAAGACTGACGTATCTACCATTCAAAAGCTTAAGGCTGGTCTCGCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

208

Amino Acids

22.12

Weight (kDa)

4.78

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 62 - 197 5.9e-32 Cupin
Cupin_2 PF07883 87 - 160 7.6e-11 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 360
AciI CCGC 2 cut(s) 216, 336
AclWI GGATC 2 cut(s) 77, 176
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 2 cut(s) 26, 266
AcuI CTGAAG 2 cut(s) 224, 377
AfaI GTAC 2 cut(s) 261, 432
AfiI CCNNNNNNNGG 1 cut(s) 119
AflII CTTAAG 1 cut(s) 599
AgsI TTSAA 6 cut(s) 18, 173, 266, 347, 571, 593
AluBI AGCT 3 cut(s) 319, 467, 598
AluI AGCT 3 cut(s) 319, 467, 598
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 2 cut(s) 77, 176
AlwNI CAGNNNCTG 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 2 cut(s) 3, 75
ApoI RAATTY 2 cut(s) 26, 266
AspLEI GCGC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 7
BaeI ACNNNNGTAYC 2 cut(s) 564, 597
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BccI CCATC 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 614
BfmI CTRYAG 1 cut(s) 468
BfrI CTTAAG 1 cut(s) 599
BlpI GCTNAGC 1 cut(s) 315
BmeT110I CYCGRG 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 340
Bpu1102I GCTNAGC 1 cut(s) 315
Bsa29I ATCGAT 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 1 cut(s) 420
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 152
Bse3DI GCAATG 2 cut(s) 78, 105
BseCI ATCGAT 1 cut(s) 543
BseDI CCNNGG 1 cut(s) 420
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 2 cut(s) 78, 105
BseMII CTCAG 4 cut(s) 210, 306, 398, 468
BseRI GAGGAG 1 cut(s) 311
BseYI CCCAGC 1 cut(s) 463
BsgI GTGCAG 1 cut(s) 519
BshFI GGCC 2 cut(s) 5, 77
BshVI ATCGAT 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 2 cut(s) 78, 153
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 2 cut(s) 5, 77
Bso31I GGTCTC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 321
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 2 cut(s) 82, 181
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 2 cut(s) 216, 336
BspANI GGCC 2 cut(s) 5, 77
BspCNI CTCAG 4 cut(s) 211, 307, 397, 467
BspDI ATCGAT 1 cut(s) 543
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 2 cut(s) 77, 176
BspTI CTTAAG 1 cut(s) 599
BspTNI GGTCTC 1 cut(s) 614
BsrDI GCAATG 2 cut(s) 78, 105
BsrFI RCCGGY 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 2 cut(s) 82, 181
BssT1I CCWWGG 1 cut(s) 420
Bst4CI ACNGT 3 cut(s) 127, 199, 508
Bst6I CTCTTC 2 cut(s) 329, 396
BstAFI CTTAAG 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 479
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 5 cut(s) 95, 219, 315, 384, 454
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 1 cut(s) 301
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 85, 184
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 2 cut(s) 82, 181
BstMWI GCNNNNNNNGC 4 cut(s) 68, 215, 479, 611
BstNSI RCATGY 1 cut(s) 160
BstPI GGTNACC 1 cut(s) 401
BstSFI CTRYAG 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
Bsu15I ATCGAT 1 cut(s) 543
BsuRI GGCC 2 cut(s) 5, 77
BsuTUI ATCGAT 1 cut(s) 543
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 158
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 1 cut(s) 152
ClaI ATCGAT 1 cut(s) 543
Csp6I GTAC 2 cut(s) 260, 431
CviAII CATG 2 cut(s) 157, 624
CviQI GTAC 2 cut(s) 260, 431
DdeI CTNAG 5 cut(s) 95, 219, 315, 384, 454
DpnI GATC 2 cut(s) 84, 183
DpnII GATC 2 cut(s) 82, 181
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 329, 396
EarI CTCTTC 2 cut(s) 329, 396
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 614
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 224, 377
Eco88I CYCGRG 1 cut(s) 7
Eco91I GGTNACC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 2 cut(s) 160, 627
FaiI YATR 6 cut(s) 148, 158, 253, 255, 470, 625
FaqI GGGAC 1 cut(s) 438
FatI CATG 2 cut(s) 156, 623
FauNDI CATATG 1 cut(s) 253
FblI GTMKAC 1 cut(s) 360
FokI GGATG 1 cut(s) 288
FriOI GRGCYC 1 cut(s) 321
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 467
HaeIII GGCC 2 cut(s) 5, 77
HapII CCGG 2 cut(s) 78, 153
HhaI GCGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 160, 627
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 2 cut(s) 388, 452
HpaII CCGG 2 cut(s) 78, 153
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 361, 433
Hpy188I TCNGA 4 cut(s) 204, 243, 387, 457
Hpy188III TCNNGA 4 cut(s) 9, 86, 173, 571
Hpy8I GTNNAC 2 cut(s) 361, 433
HpyAV CCTTC 4 cut(s) 199, 282, 528, 577
HpyCH4III ACNGT 3 cut(s) 127, 199, 508
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 3 cut(s) 56, 494, 500
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 215, 479, 611
HpyF3I CTNAG 5 cut(s) 95, 219, 315, 384, 454
HpySE526I ACGT 1 cut(s) 579
Hsp92II CATG 2 cut(s) 160, 627
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 2 cut(s) 82, 181
LmnI GCTCC 2 cut(s) 324, 619
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 2 cut(s) 98, 401
MalI GATC 2 cut(s) 84, 183
MboI GATC 2 cut(s) 82, 181
MboII GAAGA 5 cut(s) 22, 133, 188, 316, 383
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 4 cut(s) 26, 266, 474, 513
MlyI GAGTC 2 cut(s) 382, 446
MnlI CCTC 6 cut(s) 16, 223, 303, 315, 332, 386
MseI TTAA 2 cut(s) 234, 600
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 218
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 2 cut(s) 78, 153
MwoI GCNNNNNNNGC 4 cut(s) 68, 215, 479, 611
NdeI CATATG 1 cut(s) 253
NdeII GATC 2 cut(s) 82, 181
NlaIII CATG 2 cut(s) 160, 627
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 2 cut(s) 98, 401
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PaeR7I CTCGAG 1 cut(s) 7
PflFI GACNNNGTC 1 cut(s) 551
PleI GAGTC 2 cut(s) 382, 446
PpsI GAGTC 2 cut(s) 382, 446
Psp124BI GAGCTC 1 cut(s) 321
PspEI GGTNACC 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 1 cut(s) 82
PsyI GACNNNGTC 1 cut(s) 551
RsaI GTAC 2 cut(s) 261, 432
RsaNI GTAC 2 cut(s) 260, 431
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 321
SalI GTCGAC 1 cut(s) 359
SaqAI TTAA 2 cut(s) 234, 600
Sau3AI GATC 2 cut(s) 82, 181
SchI GAGTC 2 cut(s) 382, 446
SduI GDGCHC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 468
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 7, 599
SmoI CTYRAG 2 cut(s) 7, 599
SphI GCATGC 1 cut(s) 160
Sse9I AATT 4 cut(s) 26, 266, 474, 513
SsiI CCGC 2 cut(s) 216, 336
SstI GAGCTC 1 cut(s) 321
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 3 cut(s) 127, 199, 508
TaiI ACGT 1 cut(s) 582
TaqI TCGA 3 cut(s) 8, 360, 543
TasI AATT 4 cut(s) 26, 266, 474, 513
TatI WGTACW 2 cut(s) 259, 430
Tru1I TTAA 2 cut(s) 234, 600
Tru9I TTAA 2 cut(s) 234, 600
TseFI GTSAC 2 cut(s) 98, 401
Tsp45I GTSAC 2 cut(s) 98, 401
TspDTI ATGAA 2 cut(s) 94, 240
Tth111I GACNNNGTC 1 cut(s) 551
Vha464I CTTAAG 1 cut(s) 599
XapI RAATTY 2 cut(s) 26, 266
XceI RCATGY 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.