FvH4_3g25340

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000346
Physical Location & Seq
Forward (+)
18278584 .. 18279339
756 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25340.t1

Sequence Viewer

Length: 624 bp
ATGGCTTCAAGAACGTCAAGCCTCAAATTCTTCTCACTACTGATTTCCTCACTTACCATTGTTCAAATGGCAATTGCAGACCCAGACATTCTTACCGACTTCATTGCCCCTGCAAATGGAACAGTAGATGGAAACTTCTTCACATTCACCGGAATGCGAGTTCTTGTTAGTGGAGACGAGCCTAAAAACTTCACAGTATTGAAGGCAACCATGGCAGAGTTTCCTGCTCTCAATGGGCAGAGTGTTTCATACGCAGTTCTTGAATTTCCAGCTGGAACTACTAACCCACCACACACTCATCCTCGCTCTGCTGAGCTCCTCTTCCTTGTCGACGGCACCCTTGAAGTTGGCTTTGTCGACTCAAAGAACAAACTCTTTACTCAGACTCTTCAGTCAGGTGACCTGTTTGTGTTTCCCAAGGGACTTGTGCACTTCCAATACAATGCTGACTCACAAAACGCAGCTCTAGCAATTTCTGCTTTTGGAAGTGCAAGTGCAGGAACTGTGTCACTTCCTTCAACTTTGTTCGCCACCAACATCGATGACAATGTGTTGGCTTTGTCCTTCAAGACTGATGTAGCCACCATTCAAAAGCTCAAGACTGGTCTTGCTCCCAAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

208

Amino Acids

22.0

Weight (kDa)

5.23

Isoelectric Point (pI)

24.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 60 - 196 1.1e-31 Cupin
Cupin_2 PF07883 86 - 155 8.7e-12 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 335
AccI GTMKAC 2 cut(s) 330, 357
AcsI RAATTY 2 cut(s) 26, 263
AcuI CTGAAG 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 8 cut(s) 9, 65, 202, 263, 344, 519, 568, 590
AloI GAACNNNNNNTCC 2 cut(s) 144, 176
AluBI AGCT 4 cut(s) 272, 316, 464, 595
AluI AGCT 4 cut(s) 272, 316, 464, 595
Alw21I GWGCWC 2 cut(s) 318, 432
Alw26I GTCTC 1 cut(s) 168
Alw44I GTGCAC 1 cut(s) 428
AlwNI CAGNNNCTG 1 cut(s) 503
ApaLI GTGCAC 1 cut(s) 428
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 2 cut(s) 26, 263
AsuHPI GGTGA 2 cut(s) 139, 410
BaeGI GKGCMC 1 cut(s) 432
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 318
Bbv12I GWGCWC 2 cut(s) 318, 432
BbvI GCAGC 1 cut(s) 473
BccI CCATC 1 cut(s) 122
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 467
BisI GCNGC 1 cut(s) 462
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 463
BmiI GGNNCC 1 cut(s) 337
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 581
Bsa29I ATCGAT 1 cut(s) 540
BsaJI CCNNGG 2 cut(s) 210, 417
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 607
Bse3DI GCAATG 1 cut(s) 102
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 210, 417
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 2 cut(s) 303, 395
BseNI ACTGG 1 cut(s) 607
BseRI GAGGAG 1 cut(s) 308
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 1 cut(s) 473
BsgI GTGCAG 1 cut(s) 516
BshNI GGYRCC 1 cut(s) 335
BshVI ATCGAT 1 cut(s) 540
BsiHKAI GWGCWC 2 cut(s) 318, 432
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 168
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 435
BsmI GAATGC 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 318, 432
Bsp1720I GCTNAGC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 210
BspCNI CTCAG 2 cut(s) 304, 394
BspDI ATCGAT 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 2 cut(s) 210, 417
BssT1I CCWWGG 2 cut(s) 210, 417
Bst4CI ACNGT 3 cut(s) 124, 196, 505
Bst6I CTCTTC 2 cut(s) 326, 393
BstAPI GCANNNNNTGC 1 cut(s) 476
BstDEI CTNAG 2 cut(s) 312, 381
BstDSI CCRYGG 1 cut(s) 210
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 1 cut(s) 298
BstMAI GTCTC 1 cut(s) 168
BstMWI GCNNNNNNNGC 3 cut(s) 212, 467, 476
BstPI GGTNACC 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 432
BstV1I GCAGC 1 cut(s) 473
Bsu15I ATCGAT 1 cut(s) 540
BsuTUI ATCGAT 1 cut(s) 540
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 1 cut(s) 298
CaiI CAGNNNCTG 1 cut(s) 503
ClaI ATCGAT 1 cut(s) 540
CviAII CATG 1 cut(s) 211
DdeI CTNAG 2 cut(s) 312, 381
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 2 cut(s) 326, 393
EarI CTCTTC 2 cut(s) 326, 393
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 2 cut(s) 210, 417
Eco24I GRGCYC 1 cut(s) 318
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 374
Eco91I GGTNACC 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 316
EcoO65I GGTNACC 1 cut(s) 398
EcoT14I CCWWGG 2 cut(s) 210, 417
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 2 cut(s) 210, 417
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 1 cut(s) 214
FaiI YATR 3 cut(s) 212, 250, 622
FaqI GGGAC 1 cut(s) 435
FatI CATG 1 cut(s) 210
FblI GTMKAC 2 cut(s) 330, 357
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 1 cut(s) 285
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 1 cut(s) 467
GluI GCNGC 1 cut(s) 462
HapII CCGG 1 cut(s) 150
Hin1II CATG 1 cut(s) 214
HincII GTYRAC 2 cut(s) 331, 358
HindII GTYRAC 2 cut(s) 331, 358
HinfI GANTC 3 cut(s) 359, 385, 449
HpaII CCGG 1 cut(s) 150
HphI GGTGA 2 cut(s) 139, 410
Hpy166II GTNNAC 3 cut(s) 331, 358, 430
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 4 cut(s) 9, 260, 568, 598
Hpy8I GTNNAC 3 cut(s) 331, 358, 430
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 3 cut(s) 196, 525, 574
HpyCH4III ACNGT 3 cut(s) 124, 196, 505
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 5 cut(s) 77, 113, 430, 491, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 467, 476
HpyF3I CTNAG 2 cut(s) 312, 381
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 1 cut(s) 214
LmnI GCTCC 2 cut(s) 321, 616
Lsp1109I GCAGC 1 cut(s) 473
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 2 cut(s) 398, 507
MboII GAAGA 4 cut(s) 22, 130, 313, 380
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 2 cut(s) 318, 432
MluCI AATT 4 cut(s) 26, 72, 263, 471
MlyI GAGTC 3 cut(s) 353, 379, 443
MnlI CCTC 4 cut(s) 32, 58, 312, 329
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 1 cut(s) 150
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 212, 467, 476
NcoI CCATGG 1 cut(s) 210
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 2 cut(s) 398, 507
PctI GAATGC 1 cut(s) 159
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 3 cut(s) 353, 379, 443
PpsI GAGTC 3 cut(s) 353, 379, 443
Psp124BI GAGCTC 1 cut(s) 318
PspEI GGTNACC 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 272
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 318
SalI GTCGAC 2 cut(s) 329, 356
SatI GCNGC 1 cut(s) 462
SchI GAGTC 3 cut(s) 353, 379, 443
SduI GDGCHC 2 cut(s) 318, 432
SetI ASST 7 cut(s) 17, 274, 318, 400, 405, 466, 597
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse9I AATT 4 cut(s) 26, 72, 263, 471
SspMI CTAG 1 cut(s) 467
SstI GAGCTC 1 cut(s) 318
StyI CCWWGG 2 cut(s) 210, 417
TaaI ACNGT 3 cut(s) 124, 196, 505
TaiI ACGT 1 cut(s) 17
TaqI TCGA 3 cut(s) 330, 357, 540
TasI AATT 4 cut(s) 26, 72, 263, 471
TseFI GTSAC 2 cut(s) 398, 507
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 2 cut(s) 398, 507
TspDTI ATGAA 2 cut(s) 91, 237
VneI GTGCAC 1 cut(s) 428
XapI RAATTY 2 cut(s) 26, 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmiI GTMKAC 2 cut(s) 330, 357
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.