Rw5G029100

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000346
Physical Location & Seq
Forward (+)
44317701 .. 44318321
621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029100.1

Sequence Viewer

Length: 621 bp
ATGGCTTCAAGAACTTCAAGCCTCAAATTCTTCTCACTACTAATTTCTTCACTTGCCATTGTTCAAATGGCAATGGCAGACCCGGACATTCTTACCGATTTCATTGCCCCAAATAATACAGTGGATGGAAACTTCTTCACATTCACGGGAATGCGTGTTCTTGTTGGTGGAGACGAGCCTAAAACCTTCACAGTATTGAAGGCAACCTTGGCAGAGTTTCCTGCTCTAAATGGGCAGAGTGTTTCATATGCAGTCCTTGAATTCCCAGCTGGCACTACTAATCCACCACACACTCATCCTCGCTCTGCCGAGCTCCTCTTCCTCATTGAGGGTGCTCTTGAAGTCGGCTTTGTCGACACAAAGAACAACCTCTTTACTCAGACTCTTCAGACAGGTGACCTGTTTGTGTTTCCCAAGGGACTTGTGCACTACCAATACAATGCTGATTCACAAAACCCTGCTCTAGCAATCTCTGCCTTTGGAAGCGCAAGTGCCGGAACTGTGTCAATCCCTACCACTTTGTTCGCCACCAACATCGATGACAATGTCTTGGCTTTGTCCTTCAAGACTGATGCAGCCACCATTCAAAAGCTCAAGGCTGGTCTTGCTCCCAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

206

Amino Acids

21.9

Weight (kDa)

5.03

Isoelectric Point (pI)

25.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 61 - 195 1.4e-31 Cupin
Cupin_2 PF07883 85 - 158 5.6e-13 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 354
AcsI RAATTY 2 cut(s) 26, 260
AcuI CTGAAG 1 cut(s) 371
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 8 cut(s) 9, 18, 65, 199, 260, 341, 565, 587
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 3 cut(s) 269, 313, 592
AluI AGCT 3 cut(s) 269, 313, 592
Alw21I GWGCWC 3 cut(s) 315, 337, 429
Alw26I GTCTC 1 cut(s) 165
Alw44I GTGCAC 1 cut(s) 425
ApaLI GTGCAC 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 2 cut(s) 26, 260
AspLEI GCGC 1 cut(s) 488
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 429
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 3 cut(s) 315, 337, 429
BbvI GCAGC 1 cut(s) 587
BccI CCATC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 1 cut(s) 464
BisI GCNGC 1 cut(s) 576
BlsI GCNGC 1 cut(s) 577
Bme1390I CCNGG 1 cut(s) 83
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 562
BpuEI CTTGAG 1 cut(s) 578
BpuMI CCSGG 1 cut(s) 83
Bsa29I ATCGAT 1 cut(s) 537
BsaJI CCNNGG 3 cut(s) 207, 414, 612
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse3DI GCAATG 2 cut(s) 78, 102
BseCI ATCGAT 1 cut(s) 537
BseDI CCNNGG 3 cut(s) 207, 414, 612
BseGI GGATG 2 cut(s) 130, 295
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 2 cut(s) 78, 102
BseMII CTCAG 1 cut(s) 392
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 429
BseXI GCAGC 1 cut(s) 587
BseYI CCCAGC 1 cut(s) 265
BshVI ATCGAT 1 cut(s) 537
BsiHKAI GWGCWC 3 cut(s) 315, 337, 429
BsiSI CCGG 2 cut(s) 83, 495
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 432
BsmI GAATGC 1 cut(s) 156
Bsp1286I GDGCHC 3 cut(s) 315, 337, 429
BspCNI CTCAG 1 cut(s) 391
BspDI ATCGAT 1 cut(s) 537
BsrDI GCAATG 2 cut(s) 78, 102
BssECI CCNNGG 3 cut(s) 207, 414, 612
BssT1I CCWWGG 3 cut(s) 207, 414, 612
Bst4CI ACNGT 3 cut(s) 121, 193, 502
Bst6I CTCTTC 2 cut(s) 323, 390
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 378
BstEII GGTNACC 1 cut(s) 395
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 130, 295
BstHHI GCGC 1 cut(s) 488
BstMAI GTCTC 1 cut(s) 165
BstMWI GCNNNNNNNGC 4 cut(s) 209, 473, 605, 614
BstPI GGTNACC 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 537
BsuTUI ATCGAT 1 cut(s) 537
BtsCI GGATG 2 cut(s) 130, 295
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 271
CfoI GCGC 1 cut(s) 488
ClaI ATCGAT 1 cut(s) 537
CviAII CATG 1 cut(s) 618
DdeI CTNAG 1 cut(s) 378
Eam1104I CTCTTC 2 cut(s) 323, 390
EarI CTCTTC 2 cut(s) 323, 390
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 3 cut(s) 207, 414, 612
Eco24I GRGCYC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 371
Eco91I GGTNACC 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoO65I GGTNACC 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 260
EcoT14I CCWWGG 3 cut(s) 207, 414, 612
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 3 cut(s) 207, 414, 612
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 1 cut(s) 621
FaiI YATR 3 cut(s) 247, 249, 619
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 1 cut(s) 432
FatI CATG 1 cut(s) 617
FauNDI CATATG 1 cut(s) 247
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 576
FokI GGATG 2 cut(s) 137, 282
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 487
GluI GCNGC 1 cut(s) 576
GsaI CCCAGC 1 cut(s) 269
HapII CCGG 2 cut(s) 83, 495
HhaI GCGC 1 cut(s) 488
Hin1II CATG 1 cut(s) 621
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 2 cut(s) 382, 446
HpaII CCGG 2 cut(s) 83, 495
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 2 cut(s) 355, 427
Hpy188I TCNGA 2 cut(s) 381, 390
Hpy188III TCNNGA 3 cut(s) 9, 338, 565
Hpy8I GTNNAC 2 cut(s) 355, 427
HpyAV CCTTC 3 cut(s) 193, 196, 571
HpyCH4III ACNGT 3 cut(s) 121, 193, 502
HpyCH4V TGCA 3 cut(s) 251, 427, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 473, 605, 614
HpyF3I CTNAG 1 cut(s) 378
Hsp92II CATG 1 cut(s) 621
HspAI GCGC 1 cut(s) 486
LmnI GCTCC 2 cut(s) 318, 613
LpnPI CCDG 9 cut(s) 96, 234, 255, 279, 378, 413, 471, 508, 585
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 1 cut(s) 562
MaeI CTAG 1 cut(s) 464
MaeIII GTNAC 1 cut(s) 395
MboII GAAGA 5 cut(s) 22, 39, 127, 310, 377
MhlI GDGCHC 3 cut(s) 315, 337, 429
MluCI AATT 3 cut(s) 26, 42, 260
MlyI GAGTC 1 cut(s) 376
MnlI CCTC 6 cut(s) 32, 309, 322, 326, 332, 380
MslI CAYNNNNRTG 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 2 cut(s) 83, 495
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 156
MwoI GCNNNNNNNGC 4 cut(s) 209, 473, 605, 614
NciI CCSGG 1 cut(s) 83
NdeI CATATG 1 cut(s) 247
NlaIII CATG 1 cut(s) 621
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PctI GAATGC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 446
PflFI GACNNNGTC 1 cut(s) 545
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 315
PspEI GGTNACC 1 cut(s) 395
PspFI CCCAGC 1 cut(s) 265
PsyI GACNNNGTC 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 315
SalI GTCGAC 1 cut(s) 353
SatI GCNGC 1 cut(s) 576
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 3 cut(s) 315, 337, 429
SetI ASST 8 cut(s) 188, 209, 271, 315, 372, 397, 402, 594
SfaNI GCATC 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 3 cut(s) 26, 42, 260
SspMI CTAG 1 cut(s) 464
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 3 cut(s) 207, 414, 612
TaaI ACNGT 3 cut(s) 121, 193, 502
TaqI TCGA 2 cut(s) 354, 537
TasI AATT 3 cut(s) 26, 42, 260
TfiI GAWTC 1 cut(s) 446
TscAI CASTG 1 cut(s) 126
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 91, 234
TspRI CASTG 1 cut(s) 126
Tth111I GACNNNGTC 1 cut(s) 545
VneI GTGCAC 1 cut(s) 425
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 2 cut(s) 26, 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 354
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.