RLG00000034449

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000346
Physical Location & Seq
Forward (+)
42845895 .. 42847296
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034449

Sequence Viewer

Length: 897 bp
ATGGTTCCCAGTGGTCTTGGTCTCAACTCAAGATCTATGAGGAATTACTCTCATGCAGGCGCCCCAATTCTAATAGAAAAGATAAAATCAGTTTTTATGCACTCCTTTATCATATGTATCTATCGTGCATGTAGGCCGGCCTGCAGCAACACTACTACGTTCAACTCCAATCACACGACTGAAAACTCGATAAATAGCTCTTCATCCATCTTAGAAGTACCACATCCAACAATACAAATCAGCTTTAATACAACTATACAAGTCAATCAAATGGCCTCGAGAACTTCCACCCTCAAATTCTTCCCATTACTAATCTGTTCTCTTGCAATCACCGAAATGGCAATGGCCGGAGATCCAGATATTCTTAGTGACTTCATTGCGCCTCCAAATGGAACAGTAGATGGAAACTTCTTCACATACACCGGCATGCGTGTTTTCTTTGAAGAAGATGAGCCCAAAAACTTCACAGTTCTGAAGGCAACCTCCGCTGAGTTCCCTGCCCTTAATGGTCAGAGTGTTTCATATGCAGTACTTGAATTCCCATCTGGCACTACCAACCCACCTCACACTCATCCTCGCTCTGCTGAGCTCCTCTTCCTTGTTGACGGTACCCTTGAAGTTGGCTTTGTCGACACAAAAAACAACCTCTTTACGCAGACTCTTCAGGTTGGTGACCTGTTTGTCTTTCCTAAGGGACTTGTTCACTACCAGTACAATGCCGATTCACAAAACTCGGCTACAGCAATTTCTGCTTTTGGAAGTGCAAGTGCAGGAACTGTGTCAATTCCCTCCACTTTGTTCGCCACCAACATCGATGACAATGTCTTGGCTTTGTCCTTCAAGACTGACGTAGCTACCATTCAAAAGCTTAAGGCTGGTCTCGCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

299

Amino Acids

32.2

Weight (kDa)

5.77

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 151 - 287 2.7e-31 Cupin
Cupin_2 PF07883 177 - 248 1.2e-11 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
Acc65I GGTACC 1 cut(s) 608
AccB1I GGYRCC 2 cut(s) 59, 608
AccI GTMKAC 1 cut(s) 630
AciI CCGC 1 cut(s) 486
AclWI GGATC 1 cut(s) 347
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 296, 536
AcuI CTGAAG 2 cut(s) 494, 647
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 4 cut(s) 219, 531, 610, 713
AfiI CCNNNNNNNGG 1 cut(s) 389
AflII CTTAAG 1 cut(s) 869
AgsI TTSAA 6 cut(s) 163, 443, 536, 617, 841, 863
AluBI AGCT 5 cut(s) 198, 243, 589, 854, 868
AluI AGCT 5 cut(s) 198, 243, 589, 854, 868
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 26, 884
AlwI GGATC 1 cut(s) 347
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 4 cut(s) 134, 138, 273, 345
ApeKI GCWGC 1 cut(s) 144
ApoI RAATTY 2 cut(s) 296, 536
Asp718I GGTACC 1 cut(s) 608
AspLEI GCGC 2 cut(s) 62, 382
AsuHPI GGTGA 2 cut(s) 322, 683
AvaI CYCGRG 1 cut(s) 277
AxyI CCTNAGG 1 cut(s) 690
BanI GGYRCC 2 cut(s) 59, 608
BanII GRGCYC 2 cut(s) 456, 591
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 156
BccI CCATC 3 cut(s) 215, 395, 550
BcoDI GTCTC 2 cut(s) 26, 884
BfmI CTRYAG 2 cut(s) 142, 738
BfoI RGCGCY 1 cut(s) 63
BfrI CTTAAG 1 cut(s) 869
BglII AGATCT 1 cut(s) 32
BisI GCNGC 1 cut(s) 145
BlpI GCTNAGC 1 cut(s) 585
BlsI GCNGC 1 cut(s) 146
BmcAI AGTACT 1 cut(s) 531
BmeT110I CYCGRG 1 cut(s) 277
BmiI GGNNCC 3 cut(s) 6, 61, 610
BmrI ACTGGG 1 cut(s) 3
BmuI ACTGGG 1 cut(s) 3
Bpu1102I GCTNAGC 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 13
Bsa29I ATCGAT 1 cut(s) 813
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 2 cut(s) 26, 884
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse118I RCCGGY 2 cut(s) 136, 422
Bse1I ACTGG 2 cut(s) 9, 709
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 2 cut(s) 348, 375
BseCI ATCGAT 1 cut(s) 813
BseGI GGATG 3 cut(s) 203, 223, 571
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 2 cut(s) 348, 375
BseMII CTCAG 2 cut(s) 480, 576
BseNI ACTGG 2 cut(s) 9, 709
BseRI GAGGAG 1 cut(s) 581
BseXI GCAGC 1 cut(s) 156
BsgI GTGCAG 1 cut(s) 789
BshFI GGCC 4 cut(s) 136, 140, 275, 347
BshNI GGYRCC 2 cut(s) 59, 608
BshVI ATCGAT 1 cut(s) 813
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 3 cut(s) 137, 348, 423
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 26, 884
BsmFI GGGAC 1 cut(s) 708
BsnI GGCC 4 cut(s) 136, 140, 275, 347
Bso31I GGTCTC 2 cut(s) 26, 884
BsoBI CYCGRG 1 cut(s) 277
Bsp1286I GDGCHC 2 cut(s) 456, 591
Bsp143I GATC 2 cut(s) 32, 352
Bsp1720I GCTNAGC 1 cut(s) 585
BspACI CCGC 1 cut(s) 486
BspANI GGCC 4 cut(s) 136, 140, 275, 347
BspCNI CTCAG 2 cut(s) 481, 577
BspDI ATCGAT 1 cut(s) 813
BspLI GGNNCC 3 cut(s) 6, 61, 610
BspMAI CTGCAG 1 cut(s) 146
BspPI GGATC 1 cut(s) 347
BspQI GCTCTTC 1 cut(s) 205
BspT107I GGYRCC 2 cut(s) 59, 608
BspTI CTTAAG 1 cut(s) 869
BspTNI GGTCTC 2 cut(s) 26, 884
BsrDI GCAATG 2 cut(s) 348, 375
BsrFI RCCGGY 2 cut(s) 136, 422
BsrI ACTGG 2 cut(s) 9, 709
BssAI RCCGGY 2 cut(s) 136, 422
BssMI GATC 2 cut(s) 32, 352
BssNI GRCGYC 1 cut(s) 60
Bst4CI ACNGT 4 cut(s) 397, 469, 608, 778
Bst6I CTCTTC 3 cut(s) 205, 599, 666
BstACI GRCGYC 1 cut(s) 60
BstAFI CTTAAG 1 cut(s) 869
BstAPI GCANNNNNTGC 1 cut(s) 749
BstC8I GCNNGC 4 cut(s) 58, 138, 142, 428
BstDEI CTNAG 5 cut(s) 211, 365, 489, 585, 690
BstEII GGTNACC 1 cut(s) 671
BstF5I GGATG 3 cut(s) 203, 223, 571
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 2 cut(s) 62, 382
BstKTI GATC 2 cut(s) 35, 355
BstMAI GTCTC 2 cut(s) 26, 884
BstMBI GATC 2 cut(s) 32, 352
BstMWI GCNNNNNNNGC 3 cut(s) 485, 749, 881
BstNSI RCATGY 2 cut(s) 132, 430
BstPI GGTNACC 1 cut(s) 671
BstSFI CTRYAG 2 cut(s) 142, 738
BstV1I GCAGC 1 cut(s) 156
BstX2I RGATCY 2 cut(s) 32, 352
BstYI RGATCY 2 cut(s) 32, 352
Bsu15I ATCGAT 1 cut(s) 813
Bsu36I CCTNAGG 1 cut(s) 690
BsuRI GGCC 4 cut(s) 136, 140, 275, 347
BsuTUI ATCGAT 1 cut(s) 813
BtsCI GGATG 3 cut(s) 203, 223, 571
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 4 cut(s) 58, 138, 142, 428
CaiI CAGNNNCTG 1 cut(s) 776
CfoI GCGC 2 cut(s) 62, 382
Cfr10I RCCGGY 2 cut(s) 136, 422
ClaI ATCGAT 1 cut(s) 813
Csp6I GTAC 4 cut(s) 218, 530, 609, 712
CviAII CATG 4 cut(s) 53, 129, 427, 894
CviQI GTAC 4 cut(s) 218, 530, 609, 712
DdeI CTNAG 5 cut(s) 211, 365, 489, 585, 690
DinI GGCGCC 1 cut(s) 61
DpnI GATC 2 cut(s) 34, 354
DpnII GATC 2 cut(s) 32, 352
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 3 cut(s) 205, 599, 666
EarI CTCTTC 3 cut(s) 205, 599, 666
Ecl136II GAGCTC 1 cut(s) 589
Eco24I GRGCYC 2 cut(s) 456, 591
Eco31I GGTCTC 2 cut(s) 26, 884
Eco53kI GAGCTC 1 cut(s) 589
Eco57I CTGAAG 2 cut(s) 494, 647
Eco81I CCTNAGG 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 277
Eco91I GGTNACC 1 cut(s) 671
EcoICRI GAGCTC 1 cut(s) 589
EcoO65I GGTNACC 1 cut(s) 671
EcoRI GAATTC 1 cut(s) 536
EcoT38I GRGCYC 2 cut(s) 456, 591
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
FaeI CATG 4 cut(s) 56, 132, 430, 897
FaqI GGGAC 1 cut(s) 708
FatI CATG 4 cut(s) 52, 128, 426, 893
FauNDI CATATG 2 cut(s) 113, 523
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 1 cut(s) 145
FokI GGATG 3 cut(s) 190, 210, 558
FriOI GRGCYC 2 cut(s) 456, 591
FseI GGCCGGCC 1 cut(s) 140
Fsp4HI GCNGC 1 cut(s) 145
GlaI GCGC 2 cut(s) 61, 381
GluI GCNGC 1 cut(s) 145
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 4 cut(s) 136, 140, 275, 347
HapII CCGG 3 cut(s) 137, 348, 423
HhaI GCGC 2 cut(s) 62, 382
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 4 cut(s) 56, 132, 430, 897
Hin6I GCGC 2 cut(s) 60, 380
HinP1I GCGC 2 cut(s) 60, 380
HincII GTYRAC 2 cut(s) 604, 631
HindII GTYRAC 2 cut(s) 604, 631
HindIII AAGCTT 1 cut(s) 866
HinfI GANTC 2 cut(s) 658, 722
HpaII CCGG 3 cut(s) 137, 348, 423
HphI GGTGA 2 cut(s) 322, 683
Hpy166II GTNNAC 3 cut(s) 604, 631, 703
Hpy188I TCNGA 2 cut(s) 474, 513
Hpy188III TCNNGA 4 cut(s) 30, 279, 356, 841
Hpy8I GTNNAC 3 cut(s) 604, 631, 703
HpyAV CCTTC 2 cut(s) 469, 847
HpyCH4III ACNGT 4 cut(s) 397, 469, 608, 778
HpyCH4IV ACGT 2 cut(s) 158, 849
HpyCH4V TGCA 8 cut(s) 56, 100, 128, 144, 326, 527, 764, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 485, 749, 881
HpyF3I CTNAG 5 cut(s) 211, 365, 489, 585, 690
HpySE526I ACGT 2 cut(s) 158, 849
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 4 cut(s) 56, 132, 430, 897
HspAI GCGC 2 cut(s) 60, 380
KasI GGCGCC 1 cut(s) 59
KpnI GGTACC 1 cut(s) 612
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 2 cut(s) 32, 352
LguI GCTCTTC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 594, 889
Lsp1109I GCAGC 1 cut(s) 156
MaeII ACGT 2 cut(s) 158, 849
MaeIII GTNAC 2 cut(s) 368, 671
MalI GATC 2 cut(s) 34, 354
MboI GATC 2 cut(s) 32, 352
MboII GAAGA 7 cut(s) 192, 292, 403, 455, 458, 586, 653
MflI RGATCY 2 cut(s) 32, 352
MhlI GDGCHC 2 cut(s) 456, 591
MluCI AATT 6 cut(s) 43, 66, 296, 536, 744, 783
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 1 cut(s) 652
MmeI TCCRAC 1 cut(s) 251
MroNI GCCGGC 1 cut(s) 136
MseI TTAA 3 cut(s) 246, 504, 870
MslI CAYNNNNRTG 2 cut(s) 335, 425
MspA1I CMGCKG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 869
MspI CCGG 3 cut(s) 137, 348, 423
MwoI GCNNNNNNNGC 3 cut(s) 485, 749, 881
NaeI GCCGGC 1 cut(s) 138
NarI GGCGCC 1 cut(s) 60
NdeI CATATG 2 cut(s) 113, 523
NdeII GATC 2 cut(s) 32, 352
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 4 cut(s) 56, 132, 430, 897
NlaIV GGNNCC 3 cut(s) 6, 61, 610
NmeAIII GCCGAG 1 cut(s) 713
NmuCI GTSAC 2 cut(s) 368, 671
NspI RCATGY 2 cut(s) 132, 430
PaeI GCATGC 1 cut(s) 430
PaeR7I CTCGAG 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 205
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 1 cut(s) 722
PflFI GACNNNGTC 1 cut(s) 821
PkrI GCNGC 1 cut(s) 146
PleI GAGTC 1 cut(s) 652
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 652
Psp124BI GAGCTC 1 cut(s) 591
PspEI GGTNACC 1 cut(s) 671
PspN4I GGNNCC 3 cut(s) 6, 61, 610
PstI CTGCAG 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 2 cut(s) 32, 352
PsyI GACNNNGTC 1 cut(s) 821
RigI GGCCGGCC 1 cut(s) 140
RsaI GTAC 4 cut(s) 219, 531, 610, 713
RsaNI GTAC 4 cut(s) 218, 530, 609, 712
RseI CAYNNNNRTG 2 cut(s) 335, 425
SacI GAGCTC 1 cut(s) 591
SalI GTCGAC 1 cut(s) 629
SapI GCTCTTC 1 cut(s) 205
SaqAI TTAA 3 cut(s) 246, 504, 870
SatI GCNGC 1 cut(s) 145
Sau3AI GATC 2 cut(s) 32, 352
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 1 cut(s) 652
SduI GDGCHC 2 cut(s) 456, 591
SfcI CTRYAG 2 cut(s) 142, 738
SfoI GGCGCC 1 cut(s) 61
Sfr274I CTCGAG 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 277
SmiMI CAYNNNNRTG 2 cut(s) 335, 425
SmlI CTYRAG 3 cut(s) 28, 277, 869
SmoI CTYRAG 3 cut(s) 28, 277, 869
SphI GCATGC 1 cut(s) 430
Sse9I AATT 6 cut(s) 43, 66, 296, 536, 744, 783
SsiI CCGC 1 cut(s) 486
SspDI GGCGCC 1 cut(s) 59
SstI GAGCTC 1 cut(s) 591
TaaI ACNGT 4 cut(s) 397, 469, 608, 778
TaiI ACGT 2 cut(s) 161, 852
TaqI TCGA 4 cut(s) 188, 278, 630, 813
TasI AATT 6 cut(s) 43, 66, 296, 536, 744, 783
TatI WGTACW 2 cut(s) 529, 711
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 3 cut(s) 246, 504, 870
Tru9I TTAA 3 cut(s) 246, 504, 870
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 2 cut(s) 368, 671
TseI GCWGC 1 cut(s) 144
Tsp45I GTSAC 2 cut(s) 368, 671
TspDTI ATGAA 3 cut(s) 192, 364, 510
TspRI CASTG 1 cut(s) 16
Tth111I GACNNNGTC 1 cut(s) 821
Vha464I CTTAAG 1 cut(s) 869
XapI RAATTY 2 cut(s) 296, 536
XceI RCATGY 2 cut(s) 132, 430
XhoI CTCGAG 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 630
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.