Rh5CG346400

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000346
Physical Location & Seq
Forward (+)
39933745 .. 39934305
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG346400.1

Sequence Viewer

Length: 561 bp
ATGGCAATGGCTGGAGATCCAGATATTCTTAGTGACTTCATTGCGCCTCCAAATGGAACAGTAGATGGAAATTTCTTCACATACACCGGCATGCGTGTTGTCTTTGAAGAAGATGAGCCCAAAAACTTCACAGTTCTGAAGGCAACCTCCGCTGAGTTCCCTGCCCTTAATGGTCAGAGTGTTTCATATGCAATACTTGAATTCCCATCTGGCACTACTAACCCACCTCACACTCATCCTCGCTCTGCTGAGCTCCTCTTCCTTGTTGACGGTACCCTTGAGGTTGGCTTTGTCGACACAAAAAACAACCTCTTTACTCAGACTCTTCAGGTAGGTGACCTGTTTGTTTTTCCAAAGGGACTTGTTCACTACCAGTACAATGCCGATTCACAAAACTCGGCTACAGCAATTTCTGCTTTTGGAAGTGCAAGTGCAGGAACTGTGTCAATTCCTTCCACTTTGTTCGCCACCAACATCGATGACAACGCCTTGGCTTTGTCCTTCAAGACTGATGTAGCTACAATTCAACAGCTTAAGGCTGGTCTCGCTCCCAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

186

Amino Acids

19.8

Weight (kDa)

4.44

Isoelectric Point (pI)

24.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 39 - 174 5.5e-31 Cupin
Cupin_2 PF07883 65 - 136 5e-12 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 272
AccI GTMKAC 1 cut(s) 294
AciI CCGC 1 cut(s) 150
AclWI GGATC 1 cut(s) 11
AcsI RAATTY 2 cut(s) 70, 200
AcuI CTGAAG 2 cut(s) 158, 311
AfaI GTAC 2 cut(s) 274, 377
AfiI CCNNNNNNNGG 1 cut(s) 53
AflII CTTAAG 1 cut(s) 533
AgsI TTSAA 4 cut(s) 107, 200, 505, 527
AluBI AGCT 3 cut(s) 253, 518, 532
AluI AGCT 3 cut(s) 253, 518, 532
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 1 cut(s) 11
AlwNI CAGNNNCTG 1 cut(s) 440
ApoI RAATTY 2 cut(s) 70, 200
Asp718I GGTACC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 347
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 2 cut(s) 120, 255
Bbv12I GWGCWC 1 cut(s) 255
BccI CCATC 2 cut(s) 59, 214
BcoDI GTCTC 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 402
BfrI CTTAAG 1 cut(s) 533
BlpI GCTNAGC 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 477
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 489
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 373
Bse3DI GCAATG 2 cut(s) 12, 39
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 2 cut(s) 12, 39
BseMII CTCAG 3 cut(s) 144, 240, 332
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 453
BshNI GGYRCC 1 cut(s) 272
BshVI ATCGAT 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 548
BsmFI GGGAC 1 cut(s) 372
Bso31I GGTCTC 1 cut(s) 548
Bsp1286I GDGCHC 2 cut(s) 120, 255
Bsp143I GATC 1 cut(s) 16
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 1 cut(s) 150
BspCNI CTCAG 3 cut(s) 145, 241, 331
BspDI ATCGAT 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 274
BspPI GGATC 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 272
BspTI CTTAAG 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 12, 39
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 1 cut(s) 16
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 4 cut(s) 61, 133, 272, 442
Bst6I CTCTTC 2 cut(s) 263, 330
BstAFI CTTAAG 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 4 cut(s) 29, 153, 249, 318
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 1 cut(s) 19
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 3 cut(s) 149, 413, 545
BstNSI RCATGY 1 cut(s) 94
BstPI GGTNACC 1 cut(s) 335
BstSFI CTRYAG 1 cut(s) 402
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 477
BsuTUI ATCGAT 1 cut(s) 477
BtsCI GGATG 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 92
CaiI CAGNNNCTG 1 cut(s) 440
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 86
ClaI ATCGAT 1 cut(s) 477
Csp6I GTAC 2 cut(s) 273, 376
CviAII CATG 2 cut(s) 91, 558
CviQI GTAC 2 cut(s) 273, 376
DdeI CTNAG 4 cut(s) 29, 153, 249, 318
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 263, 330
EarI CTCTTC 2 cut(s) 263, 330
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 2 cut(s) 120, 255
Eco31I GGTCTC 1 cut(s) 548
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 2 cut(s) 158, 311
Eco91I GGTNACC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 2 cut(s) 120, 255
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 2 cut(s) 94, 561
FaiI YATR 5 cut(s) 82, 92, 187, 189, 559
FaqI GGGAC 1 cut(s) 372
FatI CATG 2 cut(s) 90, 557
FauNDI CATATG 1 cut(s) 187
FblI GTMKAC 1 cut(s) 294
FokI GGATG 1 cut(s) 222
FriOI GRGCYC 2 cut(s) 120, 255
GlaI GCGC 1 cut(s) 45
GsuI CTGGAG 1 cut(s) 33
HapII CCGG 1 cut(s) 87
HhaI GCGC 1 cut(s) 46
Hin1II CATG 2 cut(s) 94, 561
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 2 cut(s) 268, 295
HindII GTYRAC 2 cut(s) 268, 295
HinfI GANTC 2 cut(s) 322, 386
HpaII CCGG 1 cut(s) 87
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 3 cut(s) 268, 295, 367
Hpy188I TCNGA 3 cut(s) 138, 177, 321
Hpy188III TCNNGA 2 cut(s) 20, 505
Hpy8I GTNNAC 3 cut(s) 268, 295, 367
HpyAV CCTTC 3 cut(s) 133, 462, 511
HpyCH4III ACNGT 4 cut(s) 61, 133, 272, 442
HpyCH4V TGCA 3 cut(s) 191, 428, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 413, 545
HpyF3I CTNAG 4 cut(s) 29, 153, 249, 318
Hsp92II CATG 2 cut(s) 94, 561
HspAI GCGC 1 cut(s) 44
KpnI GGTACC 1 cut(s) 276
Kzo9I GATC 1 cut(s) 16
LmnI GCTCC 2 cut(s) 258, 553
LpnPI CCDG 9 cut(s) 33, 100, 174, 195, 314, 353, 386, 420, 525
MaeIII GTNAC 2 cut(s) 32, 335
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 5 cut(s) 67, 119, 122, 250, 317
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 120, 255
MluCI AATT 5 cut(s) 70, 200, 408, 447, 522
MlyI GAGTC 1 cut(s) 316
MnlI CCTC 7 cut(s) 57, 157, 237, 249, 266, 274, 320
MseI TTAA 2 cut(s) 168, 534
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 152
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 149, 413, 545
NdeI CATATG 1 cut(s) 187
NdeII GATC 1 cut(s) 16
NlaIII CATG 2 cut(s) 94, 561
NlaIV GGNNCC 1 cut(s) 274
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 32, 335
NspI RCATGY 1 cut(s) 94
PaeI GCATGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 386
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 335
PspN4I GGNNCC 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 2 cut(s) 274, 377
RsaNI GTAC 2 cut(s) 273, 376
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 1 cut(s) 255
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 168, 534
Sau3AI GATC 1 cut(s) 16
SchI GAGTC 1 cut(s) 316
SduI GDGCHC 2 cut(s) 120, 255
SfcI CTRYAG 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 278, 533
SmoI CTYRAG 2 cut(s) 278, 533
SphI GCATGC 1 cut(s) 94
Sse9I AATT 5 cut(s) 70, 200, 408, 447, 522
SsiI CCGC 1 cut(s) 150
SstI GAGCTC 1 cut(s) 255
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 4 cut(s) 61, 133, 272, 442
TaqI TCGA 2 cut(s) 294, 477
TasI AATT 5 cut(s) 70, 200, 408, 447, 522
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 168, 534
Tru9I TTAA 2 cut(s) 168, 534
TseFI GTSAC 2 cut(s) 32, 335
Tsp45I GTSAC 2 cut(s) 32, 335
TspDTI ATGAA 2 cut(s) 28, 174
Vha464I CTTAAG 1 cut(s) 533
XapI RAATTY 2 cut(s) 70, 200
XceI RCATGY 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.