Rh5BG319700

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000346
Physical Location & Seq
Forward (+)
42812469 .. 42813137
669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG319700.1

Sequence Viewer

Length: 627 bp
ATGGCTTCAAGAACTTCCAGCCTCAAATACTTCTCGCTACTAATTTTTGTACTCGCCATTGTTCAAATGACATTGGCAGGAGATCCAGACATTCTTAGTGACTTTATTGCGCCTCCAAATGGAACAGTAGATGGAAACTTCTTCACATACACCGGCATGCGTGTTCTTGTTGGAGGAGACGTGCCCAAAACCTTCACAGTACTGAAGGCAACCTTGGCTGAGTTCCCTGCTCTTAATGGGCAGAGTGTTTCATATGCCGTCCTTGAATTCCCAGCTGGGACTACTAATCCACCACACACTCACCCTCGCTCTGCTGAGCTCCTCTTCCTTATTGACGGTACCCTTGAAGTTGGCTTTGTCGACACAAAGAACAACCTCTTTACTCAGACTCTTCAGACAGGCGACCTGTTTGTGTTTCCCAAGGGCCTTGTTCACTACCAGTACAATGCCGATTCACAAAACTCGGCTCTGGCAGTTTCTGCCTTTGGAAGTGCAAGTGCCGGAACTGTGTCAATTCCTACCACTTTGTTTGCCACCAACATCGATGACAATGTCTTGGCTTTATCCTTCAAGACTGATGTAGCCACTATTCAAAAGCTCAAGGCCGGTCTTGCTCCGAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

208

Amino Acids

22.11

Weight (kDa)

5.19

Isoelectric Point (pI)

19.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 67 - 197 8.5e-31 Cupin
Cupin_2 PF07883 87 - 153 1.5e-12 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000346)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08590 FvH4_3g08600 FvH4_3g25280 FvH4_3g25310 FvH4_3g25320 FvH4_3g25340
malus_domestica MD03G1148000.v1.1 MD11G1166800.v1.1 MD11G1166900.v1.1 MD11G1167000.v1.1 MD11G1167300.v1.1 MD11G1167400.v1.1 MD11G1169200.v1.1 MD11G1169300.v1.1
prunus_persica Prupe.2G051900_v2.0.a1 Prupe.2G052000_v2.0.a1 Prupe.6G132900_v2.0.a1 Prupe.6G133000_v2.0.a1 Prupe.6G133100_v2.0.a1 Prupe.6G133200_v2.0.a1 Prupe.6G133300_v2.0.a1 Prupe.6G133400_v2.0.a1
pyrus_communis pycom02g19060 pycom11g13850 pycom11g13920 pycom11g13930 pycom13g26450
rosa_chinensis RchiOBHm_Chr5g0046741 RchiOBHm_Chr5g0046751 RchiOBHm_Chr5g0046771 RchiOBHm_Chr5g0046781 RchiOBHm_Chr5g0046791 RchiOBHm_Chr5g0046801 RchiOBHm_Chr5g0046811 RchiOBHm_Chr5g0046821
rosa_laevigata RLG00000029833 RLG00000029836 RLG00000034439 RLG00000034440 RLG00000034441 RLG00000034442 RLG00000034446 RLG00000034447 RLG00000034448 RLG00000034449 RLG00000034450
rosa_multiflora Rmu_co8272763.1_g000001 Rmu_co8405265.1_g000001 Rmu_sc0002264.1_g000001 Rmu_sc0002264.1_g000009 Rmu_sc0002264.1_g000010 Rmu_sc0002264.1_g000013 Rmu_sc0002264.1_g000024 Rmu_sc0004673.1_g000002 Rmu_sc0006050.1_g000018 Rmu_sc0006050.1_g000030 Rmu_sc0010683.1_g000003 Rmu_sc0010683.1_g000005 Rmu_sc0015021.1_g000001 Rmu_sc0019490.1_g000007 Rmu_sc0025079.1_g000001
rosa_roxburghii Rroxscaffold_1G00034530 Rroxscaffold_1G00034540 Rroxscaffold_1G00034550 Rroxscaffold_1G00034610 Rroxscaffold_1G00034620 Rroxscaffold_1G00034630
rosa_rugosa Rorug01G0085700 Rorug01G0085700 Rorug05G0231300 Rorug05G0231300 Rorug05G0231500 Rorug05G0231900 Rorug05G0232000 Rorug05G0232100 Rorug05G0232200
rosa_samantha Rh1AG104300 Rh1BG082600 Rh5AG311400 Rh5BG319600 Rh5BG319700 Rh5BG319800 Rh5BG319900 Rh5BG320100 Rh5BG320200 Rh5BG320400 Rh5CG345600 Rh5CG345700 Rh5CG345800 Rh5CG345900 Rh5CG346100 Rh5CG346400 Rh5CG346500 Rh5CG346600 Rh5DG330400 Rh5DG330500 Rh5DG330600 Rh5DG330700 Rh5DG330800 Rh5DG330900 Rh5DG331000 Rh5DG331100
rosa_wichuraiana Rw0G015910 Rw5G029040 Rw5G029050 Rw5G029060 Rw5G029070 Rw5G029100 Rw5G029110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 338
AccI GTMKAC 1 cut(s) 360
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 1 cut(s) 266
AcuI CTGAAG 2 cut(s) 224, 377
AfaI GTAC 4 cut(s) 51, 201, 340, 443
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 6 cut(s) 9, 65, 266, 347, 571, 593
AjiI CACGTC 1 cut(s) 181
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 3 cut(s) 275, 319, 598
AluI AGCT 3 cut(s) 275, 319, 598
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 77
AlwNI CAGNNNCTG 1 cut(s) 479
AoxI GGCC 2 cut(s) 424, 603
ApoI RAATTY 1 cut(s) 266
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 171
BlpI GCTNAGC 1 cut(s) 315
BmcAI AGTACT 1 cut(s) 201
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 1 cut(s) 340
Bpu1102I GCTNAGC 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 584
Bsa29I ATCGAT 1 cut(s) 543
BsaJI CCNNGG 2 cut(s) 213, 420
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 2 cut(s) 152, 605
Bse1I ACTGG 1 cut(s) 439
BseCI ATCGAT 1 cut(s) 543
BseDI CCNNGG 2 cut(s) 213, 420
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 3 cut(s) 210, 306, 398
BseNI ACTGG 1 cut(s) 439
BseRI GAGGAG 2 cut(s) 189, 311
BseSI GKGCMC 1 cut(s) 186
BseYI CCCAGC 2 cut(s) 271, 275
BshFI GGCC 2 cut(s) 426, 605
BshNI GGYRCC 1 cut(s) 338
BshVI ATCGAT 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 3 cut(s) 153, 501, 606
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 2 cut(s) 426, 605
Bsp1286I GDGCHC 2 cut(s) 186, 321
Bsp143I GATC 1 cut(s) 82
Bsp1720I GCTNAGC 1 cut(s) 315
BspANI GGCC 2 cut(s) 426, 605
BspCNI CTCAG 3 cut(s) 211, 307, 397
BspDI ATCGAT 1 cut(s) 543
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 338
BsrFI RCCGGY 2 cut(s) 152, 605
BsrI ACTGG 1 cut(s) 439
BssAI RCCGGY 2 cut(s) 152, 605
BssECI CCNNGG 2 cut(s) 213, 420
BssMI GATC 1 cut(s) 82
BssT1I CCWWGG 2 cut(s) 213, 420
Bst4CI ACNGT 4 cut(s) 127, 199, 338, 508
Bst6I CTCTTC 2 cut(s) 329, 396
BstAPI GCANNNNNTGC 1 cut(s) 479
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 4 cut(s) 95, 219, 315, 384
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 215, 479, 611
BstNSI RCATGY 1 cut(s) 160
BstSLI GKGCMC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
Bsu15I ATCGAT 1 cut(s) 543
BsuRI GGCC 2 cut(s) 426, 605
BsuTUI ATCGAT 1 cut(s) 543
BtrI CACGTC 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 158
CaiI CAGNNNCTG 1 cut(s) 479
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 2 cut(s) 152, 605
Cfr13I GGNCC 1 cut(s) 424
ClaI ATCGAT 1 cut(s) 543
Csp6I GTAC 4 cut(s) 50, 200, 339, 442
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviAII CATG 2 cut(s) 157, 624
CviQI GTAC 4 cut(s) 50, 200, 339, 442
DdeI CTNAG 4 cut(s) 95, 219, 315, 384
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 329, 396
EarI CTCTTC 2 cut(s) 329, 396
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 213, 420
Eco24I GRGCYC 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 224, 377
EcoICRI GAGCTC 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 266
EcoT14I CCWWGG 2 cut(s) 213, 420
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 2 cut(s) 213, 420
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 2 cut(s) 160, 627
FaiI YATR 5 cut(s) 148, 158, 253, 255, 625
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 1 cut(s) 292
FatI CATG 2 cut(s) 156, 623
FauNDI CATATG 1 cut(s) 253
FblI GTMKAC 1 cut(s) 360
FriOI GRGCYC 1 cut(s) 321
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 2 cut(s) 275, 279
HaeIII GGCC 2 cut(s) 426, 605
HapII CCGG 3 cut(s) 153, 501, 606
HhaI GCGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 160, 627
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 2 cut(s) 388, 452
HpaII CCGG 3 cut(s) 153, 501, 606
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 2 cut(s) 361, 433
Hpy188I TCNGA 3 cut(s) 387, 396, 618
Hpy188III TCNNGA 3 cut(s) 9, 86, 571
Hpy8I GTNNAC 2 cut(s) 361, 433
HpyAV CCTTC 3 cut(s) 199, 202, 577
HpyCH4III ACNGT 4 cut(s) 127, 199, 338, 508
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 1 cut(s) 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 479, 611
HpyF3I CTNAG 4 cut(s) 95, 219, 315, 384
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 2 cut(s) 160, 627
HspAI GCGC 1 cut(s) 110
KpnI GGTACC 1 cut(s) 342
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 324, 619
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 3 cut(s) 133, 316, 383
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 2 cut(s) 186, 321
MluCI AATT 3 cut(s) 42, 266, 513
MlyI GAGTC 1 cut(s) 382
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 6 cut(s) 32, 123, 167, 315, 332, 386
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 3 cut(s) 153, 501, 606
MwoI GCNNNNNNNGC 3 cut(s) 215, 479, 611
NdeI CATATG 1 cut(s) 253
NdeII GATC 1 cut(s) 82
NlaIII CATG 2 cut(s) 160, 627
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 443
NmuCI GTSAC 1 cut(s) 98
NspI RCATGY 1 cut(s) 160
PaeI GCATGC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 452
PflFI GACNNNGTC 1 cut(s) 551
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 321
PspFI CCCAGC 2 cut(s) 271, 275
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 424
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 82
PsyI GACNNNGTC 1 cut(s) 551
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 4 cut(s) 51, 201, 340, 443
RsaNI GTAC 4 cut(s) 50, 200, 339, 442
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 321
SalI GTCGAC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 234
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 424
ScaI AGTACT 1 cut(s) 201
SchI GAGTC 1 cut(s) 382
SduI GDGCHC 2 cut(s) 186, 321
SetI ASST 8 cut(s) 183, 194, 215, 277, 321, 378, 408, 600
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SphI GCATGC 1 cut(s) 160
Sse9I AATT 3 cut(s) 42, 266, 513
SstI GAGCTC 1 cut(s) 321
StyI CCWWGG 2 cut(s) 213, 420
TaaI ACNGT 4 cut(s) 127, 199, 338, 508
TaiI ACGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 360, 543
TasI AATT 3 cut(s) 42, 266, 513
TatI WGTACW 3 cut(s) 49, 199, 441
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseFI GTSAC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 1 cut(s) 240
Tth111I GACNNNGTC 1 cut(s) 551
XapI RAATTY 1 cut(s) 266
XceI RCATGY 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 360
ZrmI AGTACT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.