FvH4_4g35743

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000127
Physical Location & Seq
Reverse (-)
32960458 .. 32961106
649 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35743.t1

Sequence Viewer

Length: 630 bp
ATGTCTACGCAACCAAGTTGTGATGAATCTCCTATCAACTTAGAAAAAGATGTTGATGAAGTTGCTCCCGACCCGAATGTGCGACCTATAGGAAGAAAGGCTGCAAAGGAAGCACACCGAAAAGGGAAGGCGGCCAAAGAAGAATCACCTGTTGCAGCTGTTGTCGTGACGCTTGCAAACAGCCAAGCATCAGTCTTGTCAGCAAGGGAAAAACGAGATGAAGCATACGCAAGGCAATTGCAAGACCAACAACAACGAGAATATATGCGGCTCCAAATGGATATGCGGAATGAGCAATTCAAAATCCAAGAGAGGGAAGAGCGTATCATGGAGATGAATCCAAGCACTATGTCACCAGGGCCTAGGAAAGATTATTGGCTAGCAAAACAAAAGGCGATAGCGGAGACACAAGCAAATTTGTCGGGGTCTTTCCCCGGAGGATATCCCCAACCATCTTTCCCCGGAGGATATCCCCAAACACCCTATCCTGGTGGGTGTTACCAACCATCTTTTCCCGGTTACTATTATCCCCAACCACCTTCCACCGATGAATCTACAAACCCCATTGATGACAATGATCCTTCAAACACCAATTTCTTTTGTAATGAGGATAATAATCCCGATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.45

Weight (kDa)

4.83

Isoelectric Point (pI)

59.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 14 - 133 4.8e-10 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000127)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02381 FvH4_1g19463 FvH4_1g28433 FvH4_2g16143 FvH4_2g18491 FvH4_2g27711 FvH4_3g19631 FvH4_3g23573 FvH4_4g35743 FvH4_5g23701 FvH4_6g19603 FvH4_6g39781 FvH4_6g40561 FvH4_7g02563 FvH4_7g11472 FvH4_7g17530
rosa_chinensis RchiOBHm_Chr1g0315991 RchiOBHm_Chr1g0318371 RchiOBHm_Chr1g0328341 RchiOBHm_Chr1g0331431 RchiOBHm_Chr1g0344801 RchiOBHm_Chr1g0371461 RchiOBHm_Chr1g0379261 RchiOBHm_Chr2g0117661 RchiOBHm_Chr5g0011791 RchiOBHm_Chr5g0020991 RchiOBHm_Chr5g0060631 RchiOBHm_Chr5g0064181 RchiOBHm_Chr5g0080181 RchiOBHm_Chr6g0254691 RchiOBHm_Chr6g0260101 RchiOBHm_Chr6g0290901 RchiOBHm_Chr7g0201691
rosa_laevigata RLG00000003529 RLG00000009370 RLG00000013568
rosa_multiflora Rmu_co8437743.1_g000001 Rmu_sc0000063.1_g000028 Rmu_sc0000098.1_g000004 Rmu_sc0000166.1_g000028 Rmu_sc0000258.1_g000102 Rmu_sc0000288.1_g000018 Rmu_sc0000333.1_g000043 Rmu_sc0000451.1_g000011 Rmu_sc0000455.1_g000003 Rmu_sc0000479.1_g000002 Rmu_sc0000687.1_g000014 Rmu_sc0000733.1_g000024 Rmu_sc0000736.1_g000001 Rmu_sc0000745.1_g000017 Rmu_sc0000760.1_g000031 Rmu_sc0000776.1_g000044 Rmu_sc0000828.1_g000011 Rmu_sc0000830.1_g000027 Rmu_sc0000857.1_g000025 Rmu_sc0000864.1_g000013 Rmu_sc0001016.1_g000034 Rmu_sc0001016.1_g000035 Rmu_sc0001036.1_g000015 Rmu_sc0001096.1_g000020 Rmu_sc0001145.1_g000023 Rmu_sc0001167.1_g000057 Rmu_sc0001173.1_g000030 Rmu_sc0001260.1_g000006 Rmu_sc0001266.1_g000016 Rmu_sc0001346.1_g000003 Rmu_sc0001346.1_g000004 Rmu_sc0001386.1_g000005 Rmu_sc0001501.1_g000057 Rmu_sc0001565.1_g000058 Rmu_sc0001634.1_g000047 Rmu_sc0001699.1_g000018 Rmu_sc0001700.1_g000041 Rmu_sc0001700.1_g000055 Rmu_sc0001725.1_g000005 Rmu_sc0001823.1_g000005 Rmu_sc0001877.1_g000014 Rmu_sc0001942.1_g000059 Rmu_sc0001966.1_g000033 Rmu_sc0001998.1_g000009 Rmu_sc0002011.1_g000030 Rmu_sc0002147.1_g000007 Rmu_sc0002176.1_g000005 Rmu_sc0002235.1_g000097 Rmu_sc0002324.1_g000002 Rmu_sc0002352.1_g000011 Rmu_sc0002365.1_g000022 Rmu_sc0002548.1_g000007 Rmu_sc0002890.1_g000008 Rmu_sc0002936.1_g000003 Rmu_sc0002955.1_g000016 Rmu_sc0003160.1_g000032 Rmu_sc0003452.1_g000010 Rmu_sc0003490.1_g000023 Rmu_sc0003541.1_g000014 Rmu_sc0003555.1_g000014 Rmu_sc0003759.1_g000008 Rmu_sc0003843.1_g000001 Rmu_sc0003846.1_g000005 Rmu_sc0003890.1_g000019 Rmu_sc0003953.1_g000016 Rmu_sc0003967.1_g000001 Rmu_sc0004068.1_g000002 Rmu_sc0004101.1_g000004 Rmu_sc0004298.1_g000010 Rmu_sc0004507.1_g000008 Rmu_sc0004649.1_g000015 Rmu_sc0004719.1_g000006 Rmu_sc0004751.1_g000012 Rmu_sc0004821.1_g000005 Rmu_sc0004868.1_g000003 Rmu_sc0004876.1_g000023 Rmu_sc0004932.1_g000020 Rmu_sc0005093.1_g000020 Rmu_sc0005132.1_g000019 Rmu_sc0005274.1_g000001 Rmu_sc0005381.1_g000013 Rmu_sc0005693.1_g000009 Rmu_sc0005708.1_g000011 Rmu_sc0005834.1_g000016 Rmu_sc0005966.1_g000017 Rmu_sc0006173.1_g000033 Rmu_sc0006175.1_g000012 Rmu_sc0006590.1_g000004 Rmu_sc0006695.1_g000044 Rmu_sc0007061.1_g000002 Rmu_sc0007069.1_g000023 Rmu_sc0007216.1_g000006 Rmu_sc0008124.1_g000026 Rmu_sc0008148.1_g000028 Rmu_sc0008198.1_g000004 Rmu_sc0008423.1_g000003 Rmu_sc0008441.1_g000011 Rmu_sc0008707.1_g000005 Rmu_sc0009101.1_g000008 Rmu_sc0009240.1_g000039 Rmu_sc0009414.1_g000002 Rmu_sc0009628.1_g000001 Rmu_sc0010048.1_g000007 Rmu_sc0010275.1_g000001 Rmu_sc0010338.1_g000004 Rmu_sc0010659.1_g000005 Rmu_sc0010691.1_g000001 Rmu_sc0011023.1_g000006 Rmu_sc0011533.1_g000001 Rmu_sc0011901.1_g000001 Rmu_sc0012746.1_g000042 Rmu_sc0012773.1_g000005 Rmu_sc0012952.1_g000005 Rmu_sc0014568.1_g000002 Rmu_sc0014568.1_g000003 Rmu_sc0015188.1_g000004 Rmu_sc0019714.1_g000001 Rmu_sc0020148.1_g000003 Rmu_sc0020260.1_g000003 Rmu_sc0021213.1_g000002 Rmu_sc0022616.1_g000001 Rmu_ssc0000084.1_g000006 Rmu_ssc0000116.1_g000017 Rmu_ssc0000116.1_g000018 Rmu_ssc0000189.1_g000037 Rmu_ssc0000330.1_g000009 Rmu_ssc0000359.1_g000004 Rmu_ssc0000366.1_g000031 Rmu_ssc0000386.1_g000056 Rmu_ssc0000387.1_g000006 Rmu_ssc0000400.1_g000065 Rmu_ssc0000440.1_g000006 Rmu_ssc0000452.1_g000014
rosa_roxburghii Rroxscaffold_1G00020180 Rroxscaffold_2G00089580 Rroxscaffold_2G00143500 Rroxscaffold_3G00264360 Rroxscaffold_4G00316450 Rroxscaffold_4G00327050 Rroxscaffold_5G00350260 Rroxscaffold_5G00351750 Rroxscaffold_5G00364680 Rroxscaffold_5G00376220 Rroxscaffold_6G00391720
rosa_rugosa Rorug04G0096700 Rorug05G0284300 Rorug06G0059800 Rorug06G0059900 Rorug06G0060000 Rorug06G0060100 Rorug06G0060200 Rorug06G0127700 Rorug06G0243400
rosa_samantha Rh1AG033000 Rh1AG274600 Rh1DG069700 Rh2DG069900 Rh2DG210700 Rh2DG560100 Rh3AG245500 Rh3BG095400 Rh4AG018100 Rh4AG084900 Rh4AG177900 Rh4CG092300 Rh4CG194700 Rh5BG350600 Rh6BG362400 Rh7DG198200 Rh7DG403500
rosa_wichuraiana Rw1G008260 Rw1G035940 Rw2G027180 Rw4G003470 Rw4G010610 Rw5G037240 Rw6G036490 Rw7G015690 Rw7G028950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AciI CCGC 4 cut(s) 131, 268, 286, 401
AclWI GGATC 1 cut(s) 572
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 415
AfiI CCNNNNNNNGG 2 cut(s) 313, 488
AgsI TTSAA 2 cut(s) 301, 585
AjnI CCWGG 2 cut(s) 355, 487
AjuI GAANNNNNNNTTGG 2 cut(s) 495, 527
AluBI AGCT 1 cut(s) 158
AluI AGCT 1 cut(s) 158
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 1 cut(s) 572
AoxI GGCC 2 cut(s) 132, 359
ApeKI GCWGC 2 cut(s) 101, 155
ApoI RAATTY 1 cut(s) 415
AspA2I CCTAGG 1 cut(s) 362
AspS9I GGNCC 1 cut(s) 359
AsuC2I CCSGG 3 cut(s) 435, 462, 516
AsuHPI GGTGA 2 cut(s) 138, 345
AsuNHI GCTAGC 1 cut(s) 379
AvrII CCTAGG 1 cut(s) 362
BbvI GCAGC 2 cut(s) 88, 167
BccI CCATC 2 cut(s) 460, 514
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciT130I CCWGG 2 cut(s) 357, 489
BcnI CCSGG 3 cut(s) 435, 462, 516
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 2 cut(s) 363, 380
BfmI CTRYAG 1 cut(s) 87
BisI GCNGC 4 cut(s) 102, 132, 156, 269
BlnI CCTAGG 1 cut(s) 362
BlsI GCNGC 4 cut(s) 103, 133, 157, 270
Bme1390I CCNGG 5 cut(s) 357, 435, 462, 489, 516
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 5 cut(s) 357, 435, 462, 489, 516
BmsI GCATC 1 cut(s) 197
BmtI GCTAGC 1 cut(s) 383
BpuMI CCSGG 3 cut(s) 435, 462, 516
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 4 cut(s) 356, 362, 433, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 488
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 2 cut(s) 357, 489
BseDI CCNNGG 4 cut(s) 356, 362, 433, 460
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 2 cut(s) 313, 488
BseXI GCAGC 2 cut(s) 88, 167
BshFI GGCC 2 cut(s) 134, 361
BsiSI CCGG 3 cut(s) 435, 462, 516
BslI CCNNNNNNNGG 2 cut(s) 313, 488
BsmAI GTCTC 1 cut(s) 398
BsnI GGCC 2 cut(s) 134, 361
Bsp143I GATC 1 cut(s) 577
BspACI CCGC 4 cut(s) 131, 268, 286, 401
BspANI GGCC 2 cut(s) 134, 361
BspLI GGNNCC 1 cut(s) 272
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 572
BspQI GCTCTTC 1 cut(s) 312
BssECI CCNNGG 4 cut(s) 356, 362, 433, 460
BssMI GATC 1 cut(s) 577
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 2 cut(s) 357, 489
Bst6I CTCTTC 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 174, 381
BstDEI CTNAG 1 cut(s) 40
BstKTI GATC 1 cut(s) 580
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 1 cut(s) 577
BstMWI GCNNNNNNNGC 2 cut(s) 110, 292
BstNI CCWGG 2 cut(s) 357, 489
BstSCI CCNGG 5 cut(s) 355, 433, 460, 487, 514
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 88, 167
BsuRI GGCC 2 cut(s) 134, 361
Cac8I GCNNGC 2 cut(s) 174, 381
Cfr13I GGNCC 1 cut(s) 359
CseI GACGC 1 cut(s) 178
CviAII CATG 1 cut(s) 328
CviJI RGCY 7 cut(s) 101, 134, 158, 183, 271, 361, 379
CviKI_1 RGCY 7 cut(s) 101, 134, 158, 183, 271, 361, 379
DdeI CTNAG 1 cut(s) 40
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 362
Eco32I GATATC 2 cut(s) 443, 470
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 355, 487
EcoRV GATATC 2 cut(s) 443, 470
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 1 cut(s) 331
FaiI YATR 7 cut(s) 89, 226, 264, 266, 284, 329, 350
FatI CATG 1 cut(s) 327
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 102, 132, 156, 269
Fsp4HI GCNGC 4 cut(s) 102, 132, 156, 269
FspBI CTAG 2 cut(s) 363, 380
GluI GCNGC 4 cut(s) 102, 132, 156, 269
HaeIII GGCC 2 cut(s) 134, 361
HapII CCGG 3 cut(s) 435, 462, 516
HgaI GACGC 1 cut(s) 178
Hin1II CATG 1 cut(s) 331
HinfI GANTC 4 cut(s) 26, 143, 337, 551
HpaII CCGG 3 cut(s) 435, 462, 516
HphI GGTGA 2 cut(s) 138, 345
Hpy166II GTNNAC 1 cut(s) 6
Hpy188III TCNNGA 3 cut(s) 68, 166, 620
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 3 cut(s) 121, 549, 591
HpyCH4V TGCA 4 cut(s) 104, 155, 176, 241
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 292
HpyF3I CTNAG 1 cut(s) 40
Hsp92II CATG 1 cut(s) 331
Kzo9I GATC 1 cut(s) 577
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 70, 276
LpnPI CCDG 8 cut(s) 162, 342, 369, 448, 474, 475, 501, 529
Lsp1109I GCAGC 2 cut(s) 88, 167
LweI GCATC 1 cut(s) 197
MaeI CTAG 2 cut(s) 363, 380
MaeIII GTNAC 4 cut(s) 166, 351, 497, 518
MalI GATC 1 cut(s) 579
MboI GATC 1 cut(s) 577
MboII GAAGA 3 cut(s) 105, 152, 329
MfeI CAATTG 1 cut(s) 236
MluCI AATT 5 cut(s) 236, 296, 415, 592, 625
MnlI CCTC 4 cut(s) 306, 431, 458, 601
MslI CAYNNNNRTG 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 3 cut(s) 435, 462, 516
MspR9I CCNGG 5 cut(s) 357, 435, 462, 489, 516
MunI CAATTG 1 cut(s) 236
MvaI CCWGG 2 cut(s) 357, 489
MwoI GCNNNNNNNGC 2 cut(s) 110, 292
NciI CCSGG 3 cut(s) 435, 462, 516
NdeII GATC 1 cut(s) 577
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 1 cut(s) 331
NlaIV GGNNCC 1 cut(s) 272
NmuCI GTSAC 2 cut(s) 166, 351
PciSI GCTCTTC 1 cut(s) 312
PfeI GAWTC 4 cut(s) 26, 143, 337, 551
PkrI GCNGC 4 cut(s) 103, 133, 157, 270
Psp6I CCWGG 2 cut(s) 355, 487
PspGI CCWGG 2 cut(s) 355, 487
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 359
PvuII CAGCTG 1 cut(s) 158
RseI CAYNNNNRTG 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 312
SatI GCNGC 4 cut(s) 102, 132, 156, 269
Sau3AI GATC 1 cut(s) 577
Sau96I GGNCC 1 cut(s) 359
ScrFI CCNGG 5 cut(s) 357, 435, 462, 489, 516
SetI ASST 4 cut(s) 88, 151, 160, 541
SfaNI GCATC 1 cut(s) 197
SfcI CTRYAG 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 332
Sse9I AATT 5 cut(s) 236, 296, 415, 592, 625
SsiI CCGC 4 cut(s) 131, 268, 286, 401
SspMI CTAG 2 cut(s) 363, 380
StyD4I CCNGG 5 cut(s) 355, 433, 460, 487, 514
StyI CCWWGG 1 cut(s) 362
TasI AATT 5 cut(s) 236, 296, 415, 592, 625
TauI GCSGC 2 cut(s) 134, 271
TfiI GAWTC 4 cut(s) 26, 143, 337, 551
TseFI GTSAC 2 cut(s) 166, 351
TseI GCWGC 2 cut(s) 101, 155
Tsp45I GTSAC 2 cut(s) 166, 351
TspDTI ATGAA 5 cut(s) 39, 72, 234, 350, 564
XapI RAATTY 1 cut(s) 415
XmaJI CCTAGG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 363, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.