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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 151 |
| AcsI | RAATTY | 2 cut(s) | 374, 443 |
| AcuI | CTGAAG | 1 cut(s) | 219 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 24, 53, 228 |
| AgeI | ACCGGT | 1 cut(s) | 53 |
| AgsI | TTSAA | 5 cut(s) | 112, 175, 316, 404, 434 |
| AluBI | AGCT | 2 cut(s) | 8, 212 |
| AluI | AGCT | 2 cut(s) | 8, 212 |
| Alw26I | GTCTC | 2 cut(s) | 91, 362 |
| AlwI | GGATC | 1 cut(s) | 151 |
| ApeKI | GCWGC | 3 cut(s) | 5, 194, 209 |
| ApoI | RAATTY | 2 cut(s) | 374, 443 |
| AseI | ATTAAT | 1 cut(s) | 339 |
| AsiGI | ACCGGT | 1 cut(s) | 53 |
| AsuHPI | GGTGA | 1 cut(s) | 191 |
| BbvI | GCAGC | 3 cut(s) | 17, 181, 221 |
| BccI | CCATC | 2 cut(s) | 55, 199 |
| BclI | TGATCA | 1 cut(s) | 67 |
| BcoDI | GTCTC | 2 cut(s) | 91, 362 |
| BglII | AGATCT | 1 cut(s) | 418 |
| BisI | GCNGC | 3 cut(s) | 6, 195, 210 |
| BlsI | GCNGC | 3 cut(s) | 7, 196, 211 |
| BmsI | GCATC | 1 cut(s) | 208 |
| BpmI | CTGGAG | 1 cut(s) | 159 |
| Bpu10I | CCTNAGC | 1 cut(s) | 213 |
| BsaI | GGTCTC | 1 cut(s) | 91 |
| BsaWI | WCCGGW | 1 cut(s) | 53 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 24, 53, 228 |
| Bse118I | RCCGGY | 1 cut(s) | 53 |
| BseGI | GGATG | 2 cut(s) | 210, 223 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 24, 53, 228 |
| BseXI | GCAGC | 3 cut(s) | 17, 181, 221 |
| BshTI | ACCGGT | 1 cut(s) | 53 |
| BsiSI | CCGG | 1 cut(s) | 54 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 24, 53, 228 |
| BsmAI | GTCTC | 2 cut(s) | 91, 362 |
| Bso31I | GGTCTC | 1 cut(s) | 91 |
| Bsp143I | GATC | 4 cut(s) | 67, 143, 302, 418 |
| BspPI | GGATC | 1 cut(s) | 151 |
| BspTNI | GGTCTC | 1 cut(s) | 91 |
| BsrFI | RCCGGY | 1 cut(s) | 53 |
| BssAI | RCCGGY | 1 cut(s) | 53 |
| BssMI | GATC | 4 cut(s) | 67, 143, 302, 418 |
| BstDEI | CTNAG | 3 cut(s) | 40, 213, 261 |
| BstF5I | GGATG | 2 cut(s) | 210, 223 |
| BstKTI | GATC | 4 cut(s) | 70, 146, 305, 421 |
| BstMAI | GTCTC | 2 cut(s) | 91, 362 |
| BstMBI | GATC | 4 cut(s) | 67, 143, 302, 418 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 14, 218 |
| BstV1I | GCAGC | 3 cut(s) | 17, 181, 221 |
| BstX2I | RGATCY | 2 cut(s) | 143, 418 |
| BstYI | RGATCY | 2 cut(s) | 143, 418 |
| BtsCI | GGATG | 2 cut(s) | 210, 223 |
| Cfr10I | RCCGGY | 1 cut(s) | 53 |
| CspAI | ACCGGT | 1 cut(s) | 53 |
| CviAII | CATG | 2 cut(s) | 92, 296 |
| CviJI | RGCY | 2 cut(s) | 8, 212 |
| CviKI_1 | RGCY | 2 cut(s) | 8, 212 |
| DdeI | CTNAG | 3 cut(s) | 40, 213, 261 |
| DpnI | GATC | 4 cut(s) | 69, 145, 304, 420 |
| DpnII | GATC | 4 cut(s) | 67, 143, 302, 418 |
| Eco31I | GGTCTC | 1 cut(s) | 91 |
| Eco57I | CTGAAG | 1 cut(s) | 219 |
| EcoRI | GAATTC | 1 cut(s) | 374 |
| FaeI | CATG | 2 cut(s) | 95, 299 |
| FaiI | YATR | 7 cut(s) | 87, 93, 297, 410, 428, 452, 454 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 23, 55 |
| FatI | CATG | 2 cut(s) | 91, 295 |
| FbaI | TGATCA | 1 cut(s) | 67 |
| Fnu4HI | GCNGC | 3 cut(s) | 6, 195, 210 |
| FokI | GGATG | 2 cut(s) | 217, 230 |
| Fsp4HI | GCNGC | 3 cut(s) | 6, 195, 210 |
| GluI | GCNGC | 3 cut(s) | 6, 195, 210 |
| GsuI | CTGGAG | 1 cut(s) | 159 |
| HapII | CCGG | 1 cut(s) | 54 |
| Hin1II | CATG | 2 cut(s) | 95, 299 |
| HinfI | GANTC | 2 cut(s) | 160, 251 |
| HpaII | CCGG | 1 cut(s) | 54 |
| HphI | GGTGA | 1 cut(s) | 191 |
| Hpy188I | TCNGA | 4 cut(s) | 82, 159, 363, 380 |
| Hpy188III | TCNNGA | 2 cut(s) | 71, 128 |
| HpyAV | CCTTC | 1 cut(s) | 194 |
| HpyCH4IV | ACGT | 1 cut(s) | 289 |
| HpyCH4V | TGCA | 1 cut(s) | 169 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 14, 218 |
| HpyF3I | CTNAG | 3 cut(s) | 40, 213, 261 |
| HpySE526I | ACGT | 1 cut(s) | 289 |
| Hsp92II | CATG | 2 cut(s) | 95, 299 |
| Ksp22I | TGATCA | 1 cut(s) | 67 |
| Kzo9I | GATC | 4 cut(s) | 67, 143, 302, 418 |
| LpnPI | CCDG | 2 cut(s) | 67, 123 |
| Lsp1109I | GCAGC | 3 cut(s) | 17, 181, 221 |
| LweI | GCATC | 1 cut(s) | 208 |
| MaeII | ACGT | 1 cut(s) | 289 |
| MalI | GATC | 4 cut(s) | 69, 145, 304, 420 |
| MboI | GATC | 4 cut(s) | 67, 143, 302, 418 |
| MboII | GAAGA | 1 cut(s) | 428 |
| MfeI | CAATTG | 1 cut(s) | 170 |
| MflI | RGATCY | 2 cut(s) | 143, 418 |
| MluCI | AATT | 5 cut(s) | 63, 132, 170, 374, 443 |
| MmeI | TCCRAC | 1 cut(s) | 137 |
| MnlI | CCTC | 4 cut(s) | 17, 27, 134, 374 |
| MseI | TTAA | 2 cut(s) | 270, 339 |
| MspA1I | CMGCKG | 1 cut(s) | 8 |
| MspI | CCGG | 1 cut(s) | 54 |
| MunI | CAATTG | 1 cut(s) | 170 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 14, 218 |
| NdeII | GATC | 4 cut(s) | 67, 143, 302, 418 |
| NlaIII | CATG | 2 cut(s) | 95, 299 |
| NmeAIII | GCCGAG | 1 cut(s) | 356 |
| PfeI | GAWTC | 2 cut(s) | 160, 251 |
| PinAI | ACCGGT | 1 cut(s) | 53 |
| PkrI | GCNGC | 3 cut(s) | 7, 196, 211 |
| PshBI | ATTAAT | 1 cut(s) | 339 |
| PsuI | RGATCY | 2 cut(s) | 143, 418 |
| PvuII | CAGCTG | 1 cut(s) | 8 |
| SaqAI | TTAA | 2 cut(s) | 270, 339 |
| SatI | GCNGC | 3 cut(s) | 6, 195, 210 |
| Sau3AI | GATC | 4 cut(s) | 67, 143, 302, 418 |
| SetI | ASST | 7 cut(s) | 10, 38, 181, 214, 292, 385, 409 |
| SfaNI | GCATC | 1 cut(s) | 208 |
| Sse9I | AATT | 5 cut(s) | 63, 132, 170, 374, 443 |
| TaiI | ACGT | 1 cut(s) | 292 |
| TaqI | TCGA | 1 cut(s) | 127 |
| TasI | AATT | 5 cut(s) | 63, 132, 170, 374, 443 |
| TfiI | GAWTC | 2 cut(s) | 160, 251 |
| Tru1I | TTAA | 2 cut(s) | 270, 339 |
| Tru9I | TTAA | 2 cut(s) | 270, 339 |
| TseI | GCWGC | 3 cut(s) | 5, 194, 209 |
| TspDTI | ATGAA | 4 cut(s) | 113, 201, 357, 429 |
| VspI | ATTAAT | 1 cut(s) | 339 |
| XapI | RAATTY | 2 cut(s) | 374, 443 |