Peptidyl-prolyl cis-trans isomerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 572 |
| Acc36I | ACCTGC | 1 cut(s) | 572 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 533 |
| AccBSI | CCGCTC | 1 cut(s) | 324 |
| AccII | CGCG | 1 cut(s) | 728 |
| AciI | CCGC | 4 cut(s) | 324, 335, 726, 728 |
| AclWI | GGATC | 1 cut(s) | 137 |
| AcoI | YGGCCR | 1 cut(s) | 33 |
| AcsI | RAATTY | 3 cut(s) | 258, 538, 752 |
| AcuI | CTGAAG | 1 cut(s) | 27 |
| AfaI | GTAC | 1 cut(s) | 317 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 35, 152, 533, 718 |
| AgsI | TTSAA | 6 cut(s) | 74, 110, 265, 272, 330, 836 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 203 |
| AjnI | CCWGG | 1 cut(s) | 532 |
| AluBI | AGCT | 1 cut(s) | 233 |
| AluI | AGCT | 1 cut(s) | 233 |
| Alw26I | GTCTC | 1 cut(s) | 770 |
| AlwI | GGATC | 1 cut(s) | 137 |
| Ama87I | CYCGRG | 1 cut(s) | 692 |
| AoxI | GGCC | 3 cut(s) | 33, 215, 608 |
| ApeKI | GCWGC | 2 cut(s) | 626, 659 |
| ApoI | RAATTY | 3 cut(s) | 258, 538, 752 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 105 |
| AspS9I | GGNCC | 2 cut(s) | 26, 216 |
| AsuC2I | CCSGG | 1 cut(s) | 376 |
| AsuHPI | GGTGA | 1 cut(s) | 596 |
| AvaI | CYCGRG | 1 cut(s) | 692 |
| AvaII | GGWCC | 1 cut(s) | 26 |
| BaeGI | GKGCMC | 1 cut(s) | 158 |
| BbvI | GCAGC | 2 cut(s) | 613, 671 |
| BccI | CCATC | 2 cut(s) | 81, 554 |
| BciT130I | CCWGG | 1 cut(s) | 534 |
| BcnI | CCSGG | 1 cut(s) | 376 |
| BcoDI | GTCTC | 1 cut(s) | 770 |
| BfaI | CTAG | 2 cut(s) | 426, 471 |
| BfuAI | ACCTGC | 1 cut(s) | 572 |
| BisI | GCNGC | 2 cut(s) | 627, 660 |
| BlsI | GCNGC | 2 cut(s) | 628, 661 |
| Bme1390I | CCNGG | 2 cut(s) | 376, 534 |
| Bme18I | GGWCC | 1 cut(s) | 26 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 203 |
| BmeT110I | CYCGRG | 1 cut(s) | 692 |
| BmgT120I | GGNCC | 2 cut(s) | 26, 216 |
| BmiI | GGNNCC | 1 cut(s) | 218 |
| BmrFI | CCNGG | 2 cut(s) | 376, 534 |
| BmsI | GCATC | 1 cut(s) | 174 |
| BpmI | CTGGAG | 1 cut(s) | 504 |
| BpuEI | CTTGAG | 1 cut(s) | 597 |
| BpuMI | CCSGG | 1 cut(s) | 376 |
| BsaJI | CCNNGG | 1 cut(s) | 726 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 35, 152, 533, 718 |
| Bse1I | ACTGG | 1 cut(s) | 29 |
| BseBI | CCWGG | 1 cut(s) | 534 |
| BseDI | CCNNGG | 1 cut(s) | 726 |
| BseGI | GGATG | 4 cut(s) | 100, 189, 384, 568 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 35, 152, 533, 718 |
| BseNI | ACTGG | 1 cut(s) | 29 |
| BseSI | GKGCMC | 1 cut(s) | 158 |
| BseXI | GCAGC | 2 cut(s) | 613, 671 |
| Bsh1236I | CGCG | 1 cut(s) | 728 |
| BshFI | GGCC | 3 cut(s) | 35, 217, 610 |
| BsiHKCI | CYCGRG | 1 cut(s) | 692 |
| BsiSI | CCGG | 3 cut(s) | 36, 146, 375 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 35, 152, 533, 718 |
| BsmAI | GTCTC | 1 cut(s) | 770 |
| BsnI | GGCC | 3 cut(s) | 35, 217, 610 |
| BsoBI | CYCGRG | 1 cut(s) | 692 |
| Bsp1286I | GDGCHC | 1 cut(s) | 158 |
| Bsp143I | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| BspACI | CCGC | 4 cut(s) | 324, 335, 726, 728 |
| BspANI | GGCC | 3 cut(s) | 35, 217, 610 |
| BspFNI | CGCG | 1 cut(s) | 728 |
| BspLI | GGNNCC | 1 cut(s) | 218 |
| BspMI | ACCTGC | 1 cut(s) | 572 |
| BspPI | GGATC | 1 cut(s) | 137 |
| BsrBI | CCGCTC | 1 cut(s) | 324 |
| BsrI | ACTGG | 1 cut(s) | 29 |
| BssECI | CCNNGG | 1 cut(s) | 726 |
| BssMI | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| Bst2UI | CCWGG | 1 cut(s) | 534 |
| Bst6I | CTCTTC | 2 cut(s) | 377, 771 |
| BstC8I | GCNNGC | 3 cut(s) | 303, 372, 730 |
| BstDSI | CCRYGG | 1 cut(s) | 726 |
| BstF5I | GGATG | 4 cut(s) | 100, 189, 384, 568 |
| BstFNI | CGCG | 1 cut(s) | 728 |
| BstKTI | GATC | 5 cut(s) | 52, 145, 230, 670, 850 |
| BstMAI | GTCTC | 1 cut(s) | 770 |
| BstMBI | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 193, 431, 635 |
| BstNI | CCWGG | 1 cut(s) | 534 |
| BstNSI | RCATGY | 2 cut(s) | 191, 374 |
| BstSCI | CCNGG | 2 cut(s) | 374, 532 |
| BstSLI | GKGCMC | 1 cut(s) | 158 |
| BstUI | CGCG | 1 cut(s) | 728 |
| BstV1I | GCAGC | 2 cut(s) | 613, 671 |
| BstX2I | RGATCY | 1 cut(s) | 142 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 553 |
| BstYI | RGATCY | 1 cut(s) | 142 |
| BsuRI | GGCC | 3 cut(s) | 35, 217, 610 |
| BtgI | CCRYGG | 1 cut(s) | 726 |
| BtsCI | GGATG | 4 cut(s) | 100, 189, 384, 568 |
| BtsIMutI | CAGTG | 1 cut(s) | 36 |
| BveI | ACCTGC | 1 cut(s) | 572 |
| Cac8I | GCNNGC | 3 cut(s) | 303, 372, 730 |
| Cfr13I | GGNCC | 2 cut(s) | 26, 216 |
| Cfr42I | CCGCGG | 1 cut(s) | 729 |
| Csp6I | GTAC | 1 cut(s) | 316 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 233, 268 |
| CviAII | CATG | 3 cut(s) | 188, 371, 559 |
| CviQI | GTAC | 1 cut(s) | 316 |
| DpnI | GATC | 5 cut(s) | 51, 144, 229, 669, 849 |
| DpnII | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| DraI | TTTAAA | 1 cut(s) | 460 |
| DriI | GACNNNNNGTC | 1 cut(s) | 203 |
| EaeI | YGGCCR | 1 cut(s) | 33 |
| Eam1104I | CTCTTC | 2 cut(s) | 377, 771 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 203 |
| EarI | CTCTTC | 2 cut(s) | 377, 771 |
| Eco147I | AGGCCT | 1 cut(s) | 610 |
| Eco47I | GGWCC | 1 cut(s) | 26 |
| Eco57I | CTGAAG | 1 cut(s) | 27 |
| Eco88I | CYCGRG | 1 cut(s) | 692 |
| EcoRII | CCWGG | 1 cut(s) | 532 |
| EcoT22I | ATGCAT | 1 cut(s) | 189 |
| FaeI | CATG | 3 cut(s) | 191, 374, 562 |
| FatI | CATG | 3 cut(s) | 187, 370, 558 |
| FauI | CCCGC | 1 cut(s) | 721 |
| Fnu4HI | GCNGC | 2 cut(s) | 627, 660 |
| FokI | GGATG | 4 cut(s) | 107, 196, 391, 575 |
| Fsp4HI | GCNGC | 2 cut(s) | 627, 660 |
| FspBI | CTAG | 2 cut(s) | 426, 471 |
| GluI | GCNGC | 2 cut(s) | 627, 660 |
| GsuI | CTGGAG | 1 cut(s) | 504 |
| HaeIII | GGCC | 3 cut(s) | 35, 217, 610 |
| HapII | CCGG | 3 cut(s) | 36, 146, 375 |
| Hin1II | CATG | 3 cut(s) | 191, 374, 562 |
| HinfI | GANTC | 1 cut(s) | 804 |
| HpaII | CCGG | 3 cut(s) | 36, 146, 375 |
| HphI | GGTGA | 1 cut(s) | 596 |
| Hpy188I | TCNGA | 2 cut(s) | 165, 417 |
| Hpy188III | TCNNGA | 5 cut(s) | 74, 483, 692, 694, 836 |
| HpyAV | CCTTC | 6 cut(s) | 243, 259, 494, 612, 616, 649 |
| HpyCH4V | TGCA | 7 cut(s) | 117, 187, 244, 293, 452, 581, 593 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 193, 431, 635 |
| Hsp92II | CATG | 3 cut(s) | 191, 374, 562 |
| KspI | CCGCGG | 1 cut(s) | 729 |
| Kzo9I | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| Lsp1109I | GCAGC | 2 cut(s) | 613, 671 |
| LweI | GCATC | 1 cut(s) | 174 |
| MaeI | CTAG | 2 cut(s) | 426, 471 |
| MaeIII | GTNAC | 1 cut(s) | 428 |
| MalI | GATC | 5 cut(s) | 51, 144, 229, 669, 849 |
| MbiI | CCGCTC | 1 cut(s) | 324 |
| MboI | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| MboII | GAAGA | 5 cut(s) | 20, 71, 113, 394, 788 |
| MfeI | CAATTG | 1 cut(s) | 110 |
| MflI | RGATCY | 1 cut(s) | 142 |
| MhlI | GDGCHC | 1 cut(s) | 158 |
| Mph1103I | ATGCAT | 1 cut(s) | 189 |
| MroXI | GAANNNNTTC | 1 cut(s) | 105 |
| MseI | TTAA | 3 cut(s) | 296, 459, 855 |
| MspA1I | CMGCKG | 1 cut(s) | 728 |
| MspI | CCGG | 3 cut(s) | 36, 146, 375 |
| MspR9I | CCNGG | 2 cut(s) | 376, 534 |
| MunI | CAATTG | 1 cut(s) | 110 |
| MvaI | CCWGG | 1 cut(s) | 534 |
| MvnI | CGCG | 1 cut(s) | 728 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 193, 431, 635 |
| NciI | CCSGG | 1 cut(s) | 376 |
| NdeII | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| NlaIII | CATG | 3 cut(s) | 191, 374, 562 |
| NlaIV | GGNNCC | 1 cut(s) | 218 |
| NsiI | ATGCAT | 1 cut(s) | 189 |
| NspI | RCATGY | 2 cut(s) | 191, 374 |
| PaeI | GCATGC | 1 cut(s) | 374 |
| PaeR7I | CTCGAG | 1 cut(s) | 692 |
| PaqCI | CACCTGC | 1 cut(s) | 572 |
| PceI | AGGCCT | 1 cut(s) | 610 |
| PdmI | GAANNNNTTC | 1 cut(s) | 105 |
| PfeI | GAWTC | 1 cut(s) | 804 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 533 |
| PfoI | TCCNGGA | 1 cut(s) | 532 |
| PkrI | GCNGC | 2 cut(s) | 628, 661 |
| Psp6I | CCWGG | 1 cut(s) | 532 |
| PspGI | CCWGG | 1 cut(s) | 532 |
| PspN4I | GGNNCC | 1 cut(s) | 218 |
| PspPI | GGNCC | 2 cut(s) | 26, 216 |
| PsuI | RGATCY | 1 cut(s) | 142 |
| RsaI | GTAC | 1 cut(s) | 317 |
| RsaNI | GTAC | 1 cut(s) | 316 |
| SacII | CCGCGG | 1 cut(s) | 729 |
| SaqAI | TTAA | 3 cut(s) | 296, 459, 855 |
| SatI | GCNGC | 2 cut(s) | 627, 660 |
| Sau3AI | GATC | 5 cut(s) | 49, 142, 227, 667, 847 |
| Sau96I | GGNCC | 2 cut(s) | 26, 216 |
| ScrFI | CCNGG | 2 cut(s) | 376, 534 |
| SduI | GDGCHC | 1 cut(s) | 158 |
| SetI | ASST | 6 cut(s) | 28, 235, 254, 270, 576, 586 |
| SfaNI | GCATC | 1 cut(s) | 174 |
| Sfr274I | CTCGAG | 1 cut(s) | 692 |
| Sfr303I | CCGCGG | 1 cut(s) | 729 |
| SgrBI | CCGCGG | 1 cut(s) | 729 |
| SinI | GGWCC | 1 cut(s) | 26 |
| SlaI | CTCGAG | 1 cut(s) | 692 |
| SmlI | CTYRAG | 2 cut(s) | 612, 692 |
| SmoI | CTYRAG | 2 cut(s) | 612, 692 |
| SphI | GCATGC | 1 cut(s) | 374 |
| SseBI | AGGCCT | 1 cut(s) | 610 |
| SsiI | CCGC | 4 cut(s) | 324, 335, 726, 728 |
| SspI | AATATT | 1 cut(s) | 70 |
| SspMI | CTAG | 2 cut(s) | 426, 471 |
| StuI | AGGCCT | 1 cut(s) | 610 |
| StyD4I | CCNGG | 2 cut(s) | 374, 532 |
| TaqI | TCGA | 4 cut(s) | 693, 760, 807, 850 |
| TfiI | GAWTC | 1 cut(s) | 804 |
| Tru1I | TTAA | 3 cut(s) | 296, 459, 855 |
| Tru9I | TTAA | 3 cut(s) | 296, 459, 855 |
| TscAI | CASTG | 1 cut(s) | 36 |
| TseI | GCWGC | 2 cut(s) | 626, 659 |
| TspDTI | ATGAA | 4 cut(s) | 72, 366, 395, 513 |
| TspGWI | ACGGA | 2 cut(s) | 107, 658 |
| TspRI | CASTG | 1 cut(s) | 36 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 533 |
| VpaK11BI | GGWCC | 1 cut(s) | 26 |
| XapI | RAATTY | 3 cut(s) | 258, 538, 752 |
| XceI | RCATGY | 2 cut(s) | 191, 374 |
| XhoI | CTCGAG | 1 cut(s) | 692 |
| XmnI | GAANNNNTTC | 1 cut(s) | 105 |
| XspI | CTAG | 2 cut(s) | 426, 471 |
| Zsp2I | ATGCAT | 1 cut(s) | 189 |