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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 143 |
| AccIII | TCCGGA | 1 cut(s) | 107 |
| AciI | CCGC | 3 cut(s) | 341, 581, 635 |
| AclI | AACGTT | 1 cut(s) | 550 |
| AclWI | GGATC | 1 cut(s) | 215 |
| AcsI | RAATTY | 2 cut(s) | 163, 365 |
| AcyI | GRCGYC | 2 cut(s) | 536, 608 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 620, 647 |
| AjnI | CCWGG | 1 cut(s) | 157 |
| Alw26I | GTCTC | 1 cut(s) | 383 |
| AlwI | GGATC | 1 cut(s) | 215 |
| Ama87I | CYCGRG | 1 cut(s) | 305 |
| Aor13HI | TCCGGA | 1 cut(s) | 107 |
| AoxI | GGCC | 1 cut(s) | 233 |
| ApeKI | GCWGC | 1 cut(s) | 272 |
| ApoI | RAATTY | 2 cut(s) | 163, 365 |
| AspLEI | GCGC | 1 cut(s) | 144 |
| AvaI | CYCGRG | 1 cut(s) | 305 |
| BbsI | GAAGAC | 1 cut(s) | 26 |
| BbvI | GCAGC | 1 cut(s) | 284 |
| BccI | CCATC | 2 cut(s) | 179, 673 |
| BciT130I | CCWGG | 1 cut(s) | 159 |
| BciVI | GTATCC | 2 cut(s) | 416, 620 |
| BcoDI | GTCTC | 1 cut(s) | 383 |
| BfaI | CTAG | 2 cut(s) | 96, 356 |
| BfmI | CTRYAG | 2 cut(s) | 216, 618 |
| BfuI | GTATCC | 2 cut(s) | 416, 620 |
| BisI | GCNGC | 1 cut(s) | 273 |
| BlsI | GCNGC | 1 cut(s) | 274 |
| Bme1390I | CCNGG | 1 cut(s) | 159 |
| BmeT110I | CYCGRG | 1 cut(s) | 305 |
| BmrFI | CCNGG | 1 cut(s) | 159 |
| BpiI | GAAGAC | 1 cut(s) | 26 |
| BpuEI | CTTGAG | 1 cut(s) | 222 |
| BsaHI | GRCGYC | 2 cut(s) | 536, 608 |
| BsaWI | WCCGGW | 1 cut(s) | 107 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 620, 647 |
| BseAI | TCCGGA | 1 cut(s) | 107 |
| BseBI | CCWGG | 1 cut(s) | 159 |
| BseGI | GGATG | 3 cut(s) | 90, 104, 193 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 620, 647 |
| BseXI | GCAGC | 1 cut(s) | 284 |
| BshFI | GGCC | 1 cut(s) | 235 |
| BsiHKCI | CYCGRG | 1 cut(s) | 305 |
| BsiSI | CCGG | 1 cut(s) | 108 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 620, 647 |
| BsmAI | GTCTC | 1 cut(s) | 383 |
| BsmI | GAATGC | 1 cut(s) | 656 |
| BsnI | GGCC | 1 cut(s) | 235 |
| BsoBI | CYCGRG | 1 cut(s) | 305 |
| Bsp13I | TCCGGA | 1 cut(s) | 107 |
| Bsp143I | GATC | 3 cut(s) | 220, 280, 460 |
| BspACI | CCGC | 3 cut(s) | 341, 581, 635 |
| BspANI | GGCC | 1 cut(s) | 235 |
| BspEI | TCCGGA | 1 cut(s) | 107 |
| BspPI | GGATC | 1 cut(s) | 215 |
| BspQI | GCTCTTC | 1 cut(s) | 474 |
| BssMI | GATC | 3 cut(s) | 220, 280, 460 |
| BssNI | GRCGYC | 2 cut(s) | 536, 608 |
| Bst2UI | CCWGG | 1 cut(s) | 159 |
| Bst4CI | ACNGT | 1 cut(s) | 217 |
| Bst6I | CTCTTC | 1 cut(s) | 474 |
| BstACI | GRCGYC | 2 cut(s) | 536, 608 |
| BstC8I | GCNNGC | 2 cut(s) | 343, 602 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 618 |
| BstF5I | GGATG | 3 cut(s) | 90, 104, 193 |
| BstHHI | GCGC | 1 cut(s) | 144 |
| BstKTI | GATC | 3 cut(s) | 223, 283, 463 |
| BstMAI | GTCTC | 1 cut(s) | 383 |
| BstMBI | GATC | 3 cut(s) | 220, 280, 460 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 433 |
| BstNI | CCWGG | 1 cut(s) | 159 |
| BstNSI | RCATGY | 1 cut(s) | 405 |
| BstSCI | CCNGG | 1 cut(s) | 157 |
| BstSFI | CTRYAG | 2 cut(s) | 216, 618 |
| BstV1I | GCAGC | 1 cut(s) | 284 |
| BstV2I | GAAGAC | 1 cut(s) | 26 |
| BstX2I | RGATCY | 1 cut(s) | 220 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 178 |
| BstYI | RGATCY | 1 cut(s) | 220 |
| BsuI | GTATCC | 2 cut(s) | 416, 620 |
| BsuRI | GGCC | 1 cut(s) | 235 |
| BtsCI | GGATG | 3 cut(s) | 90, 104, 193 |
| Cac8I | GCNNGC | 2 cut(s) | 343, 602 |
| CfoI | GCGC | 1 cut(s) | 144 |
| CseI | GACGC | 2 cut(s) | 544, 616 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 631, 666 |
| CviAII | CATG | 2 cut(s) | 184, 402 |
| CviJI | RGCY | 3 cut(s) | 72, 99, 235 |
| CviKI_1 | RGCY | 3 cut(s) | 72, 99, 235 |
| DpnI | GATC | 3 cut(s) | 222, 282, 462 |
| DpnII | GATC | 3 cut(s) | 220, 280, 460 |
| DraI | TTTAAA | 1 cut(s) | 85 |
| Eam1104I | CTCTTC | 1 cut(s) | 474 |
| EarI | CTCTTC | 1 cut(s) | 474 |
| Eco147I | AGGCCT | 1 cut(s) | 235 |
| Eco88I | CYCGRG | 1 cut(s) | 305 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 618 |
| EcoRI | GAATTC | 1 cut(s) | 365 |
| EcoRII | CCWGG | 1 cut(s) | 157 |
| FaeI | CATG | 2 cut(s) | 187, 405 |
| FaiI | YATR | 8 cut(s) | 102, 138, 140, 185, 314, 403, 512, 620 |
| FatI | CATG | 2 cut(s) | 183, 401 |
| FauI | CCCGC | 1 cut(s) | 334 |
| FauNDI | CATATG | 1 cut(s) | 138 |
| Fnu4HI | GCNGC | 1 cut(s) | 273 |
| FokI | GGATG | 3 cut(s) | 77, 91, 200 |
| Fsp4HI | GCNGC | 1 cut(s) | 273 |
| FspAI | RTGCGCAY | 1 cut(s) | 143 |
| FspBI | CTAG | 2 cut(s) | 96, 356 |
| FspI | TGCGCA | 1 cut(s) | 143 |
| GlaI | GCGC | 1 cut(s) | 143 |
| GluI | GCNGC | 1 cut(s) | 273 |
| HaeIII | GGCC | 1 cut(s) | 235 |
| HapII | CCGG | 1 cut(s) | 108 |
| HgaI | GACGC | 2 cut(s) | 544, 616 |
| HhaI | GCGC | 1 cut(s) | 144 |
| Hin1I | GRCGYC | 2 cut(s) | 536, 608 |
| Hin1II | CATG | 2 cut(s) | 187, 405 |
| Hin6I | GCGC | 1 cut(s) | 142 |
| HinP1I | GCGC | 1 cut(s) | 142 |
| HinfI | GANTC | 1 cut(s) | 683 |
| HpaII | CCGG | 1 cut(s) | 108 |
| Hpy188I | TCNGA | 1 cut(s) | 42 |
| Hpy188III | TCNNGA | 3 cut(s) | 108, 307, 561 |
| HpyAV | CCTTC | 3 cut(s) | 119, 262, 624 |
| HpyCH4III | ACNGT | 1 cut(s) | 217 |
| HpyCH4IV | ACGT | 2 cut(s) | 505, 550 |
| HpyCH4V | TGCA | 3 cut(s) | 77, 206, 656 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 433 |
| HpySE526I | ACGT | 2 cut(s) | 505, 550 |
| Hsp92I | GRCGYC | 2 cut(s) | 536, 608 |
| Hsp92II | CATG | 2 cut(s) | 187, 405 |
| HspAI | GCGC | 1 cut(s) | 142 |
| Kpn2I | TCCGGA | 1 cut(s) | 107 |
| Kzo9I | GATC | 3 cut(s) | 220, 280, 460 |
| LguI | GCTCTTC | 1 cut(s) | 474 |
| LpnPI | CCDG | 3 cut(s) | 121, 144, 171 |
| Lsp1109I | GCAGC | 1 cut(s) | 284 |
| MaeI | CTAG | 2 cut(s) | 96, 356 |
| MaeII | ACGT | 2 cut(s) | 505, 550 |
| MaeIII | GTNAC | 1 cut(s) | 53 |
| MalI | GATC | 3 cut(s) | 222, 282, 462 |
| MboI | GATC | 3 cut(s) | 220, 280, 460 |
| MboII | GAAGA | 6 cut(s) | 16, 31, 470, 491, 512, 586 |
| MflI | RGATCY | 1 cut(s) | 220 |
| MluCI | AATT | 8 cut(s) | 34, 80, 153, 163, 201, 365, 465, 643 |
| MroI | TCCGGA | 1 cut(s) | 107 |
| MseI | TTAA | 3 cut(s) | 84, 468, 700 |
| MspI | CCGG | 1 cut(s) | 108 |
| MspR9I | CCNGG | 1 cut(s) | 159 |
| Mva1269I | GAATGC | 1 cut(s) | 656 |
| MvaI | CCWGG | 1 cut(s) | 159 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 433 |
| NdeI | CATATG | 1 cut(s) | 138 |
| NdeII | GATC | 3 cut(s) | 220, 280, 460 |
| NlaIII | CATG | 2 cut(s) | 187, 405 |
| NsbI | TGCGCA | 1 cut(s) | 143 |
| NspI | RCATGY | 1 cut(s) | 405 |
| PaeR7I | CTCGAG | 1 cut(s) | 305 |
| PceI | AGGCCT | 1 cut(s) | 235 |
| PciSI | GCTCTTC | 1 cut(s) | 474 |
| PctI | GAATGC | 1 cut(s) | 656 |
| PfeI | GAWTC | 1 cut(s) | 683 |
| PfoI | TCCNGGA | 1 cut(s) | 157 |
| PkrI | GCNGC | 1 cut(s) | 274 |
| Psp1406I | AACGTT | 1 cut(s) | 550 |
| Psp6I | CCWGG | 1 cut(s) | 157 |
| PspGI | CCWGG | 1 cut(s) | 157 |
| PsuI | RGATCY | 1 cut(s) | 220 |
| SapI | GCTCTTC | 1 cut(s) | 474 |
| SaqAI | TTAA | 3 cut(s) | 84, 468, 700 |
| SatI | GCNGC | 1 cut(s) | 273 |
| Sau3AI | GATC | 3 cut(s) | 220, 280, 460 |
| ScrFI | CCNGG | 1 cut(s) | 159 |
| SetI | ASST | 7 cut(s) | 201, 508, 553, 616, 625, 673, 696 |
| SfcI | CTRYAG | 2 cut(s) | 216, 618 |
| Sfr274I | CTCGAG | 1 cut(s) | 305 |
| SlaI | CTCGAG | 1 cut(s) | 305 |
| SmlI | CTYRAG | 2 cut(s) | 237, 305 |
| SmoI | CTYRAG | 2 cut(s) | 237, 305 |
| Sse9I | AATT | 8 cut(s) | 34, 80, 153, 163, 201, 365, 465, 643 |
| SseBI | AGGCCT | 1 cut(s) | 235 |
| SsiI | CCGC | 3 cut(s) | 341, 581, 635 |
| SspMI | CTAG | 2 cut(s) | 96, 356 |
| StuI | AGGCCT | 1 cut(s) | 235 |
| StyD4I | CCNGG | 1 cut(s) | 157 |
| TaaI | ACNGT | 1 cut(s) | 217 |
| TaiI | ACGT | 2 cut(s) | 508, 553 |
| TaqI | TCGA | 4 cut(s) | 306, 373, 420, 463 |
| TasI | AATT | 8 cut(s) | 34, 80, 153, 163, 201, 365, 465, 643 |
| TfiI | GAWTC | 1 cut(s) | 683 |
| Tru1I | TTAA | 3 cut(s) | 84, 468, 700 |
| Tru9I | TTAA | 3 cut(s) | 84, 468, 700 |
| TseI | GCWGC | 1 cut(s) | 272 |
| TspDTI | ATGAA | 2 cut(s) | 17, 138 |
| TspGWI | ACGGA | 1 cut(s) | 271 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 618 |
| XapI | RAATTY | 2 cut(s) | 163, 365 |
| XceI | RCATGY | 1 cut(s) | 405 |
| XhoI | CTCGAG | 1 cut(s) | 305 |
| XspI | CTAG | 2 cut(s) | 96, 356 |