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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 3 cut(s) | 151, 415, 479 |
| AcoI | YGGCCR | 1 cut(s) | 489 |
| AcsI | RAATTY | 1 cut(s) | 416 |
| AcuI | CTGAAG | 1 cut(s) | 423 |
| AfaI | GTAC | 1 cut(s) | 460 |
| AgsI | TTSAA | 2 cut(s) | 112, 376 |
| AjnI | CCWGG | 1 cut(s) | 17 |
| AluBI | AGCT | 2 cut(s) | 8, 272 |
| AluI | AGCT | 2 cut(s) | 8, 272 |
| AlwI | GGATC | 3 cut(s) | 151, 415, 479 |
| AoxI | GGCC | 1 cut(s) | 489 |
| ApeKI | GCWGC | 2 cut(s) | 5, 269 |
| ApoI | RAATTY | 1 cut(s) | 416 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 181, 213 |
| AsuHPI | GGTGA | 2 cut(s) | 332, 458 |
| BbvI | GCAGC | 2 cut(s) | 17, 281 |
| BccI | CCATC | 1 cut(s) | 437 |
| BciT130I | CCWGG | 1 cut(s) | 19 |
| BclI | TGATCA | 2 cut(s) | 67, 184 |
| BisI | GCNGC | 2 cut(s) | 6, 270 |
| BlsI | GCNGC | 2 cut(s) | 7, 271 |
| BmcAI | AGTACT | 1 cut(s) | 460 |
| Bme1390I | CCNGG | 1 cut(s) | 19 |
| BmrFI | CCNGG | 1 cut(s) | 19 |
| BpmI | CTGGAG | 2 cut(s) | 159, 447 |
| BsaBI | GATNNNNATC | 1 cut(s) | 183 |
| Bse1I | ACTGG | 2 cut(s) | 351, 464 |
| Bse8I | GATNNNNATC | 1 cut(s) | 183 |
| BseBI | CCWGG | 1 cut(s) | 19 |
| BseGI | GGATG | 1 cut(s) | 124 |
| BseJI | GATNNNNATC | 1 cut(s) | 183 |
| BseNI | ACTGG | 2 cut(s) | 351, 464 |
| BseXI | GCAGC | 2 cut(s) | 17, 281 |
| BshFI | GGCC | 1 cut(s) | 491 |
| BsiSI | CCGG | 1 cut(s) | 488 |
| BsmI | GAATGC | 1 cut(s) | 19 |
| BsnI | GGCC | 1 cut(s) | 491 |
| Bsp143I | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| BspANI | GGCC | 1 cut(s) | 491 |
| BspPI | GGATC | 3 cut(s) | 151, 415, 479 |
| BsrI | ACTGG | 2 cut(s) | 351, 464 |
| BssMI | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| Bst2UI | CCWGG | 1 cut(s) | 19 |
| Bst4CI | ACNGT | 1 cut(s) | 476 |
| BstDEI | CTNAG | 2 cut(s) | 9, 273 |
| BstF5I | GGATG | 1 cut(s) | 124 |
| BstKTI | GATC | 7 cut(s) | 70, 146, 187, 365, 410, 487, 501 |
| BstMBI | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 14 |
| BstNI | CCWGG | 1 cut(s) | 19 |
| BstSCI | CCNGG | 1 cut(s) | 17 |
| BstV1I | GCAGC | 2 cut(s) | 17, 281 |
| BstX2I | RGATCY | 2 cut(s) | 143, 407 |
| BstYI | RGATCY | 2 cut(s) | 143, 407 |
| BsuRI | GGCC | 1 cut(s) | 491 |
| BtsCI | GGATG | 1 cut(s) | 124 |
| Csp6I | GTAC | 1 cut(s) | 459 |
| CviAII | CATG | 1 cut(s) | 356 |
| CviJI | RGCY | 3 cut(s) | 8, 272, 491 |
| CviKI_1 | RGCY | 3 cut(s) | 8, 272, 491 |
| CviQI | GTAC | 1 cut(s) | 459 |
| DdeI | CTNAG | 2 cut(s) | 9, 273 |
| DpnI | GATC | 7 cut(s) | 69, 145, 186, 364, 409, 486, 500 |
| DpnII | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| EaeI | YGGCCR | 1 cut(s) | 489 |
| Eco57I | CTGAAG | 1 cut(s) | 423 |
| EcoRII | CCWGG | 1 cut(s) | 17 |
| FaeI | CATG | 1 cut(s) | 359 |
| FaiI | YATR | 3 cut(s) | 266, 357, 440 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 23, 55 |
| FatI | CATG | 1 cut(s) | 355 |
| FbaI | TGATCA | 2 cut(s) | 67, 184 |
| Fnu4HI | GCNGC | 2 cut(s) | 6, 270 |
| FokI | GGATG | 1 cut(s) | 111 |
| Fsp4HI | GCNGC | 2 cut(s) | 6, 270 |
| GluI | GCNGC | 2 cut(s) | 6, 270 |
| GsuI | CTGGAG | 2 cut(s) | 159, 447 |
| HaeIII | GGCC | 1 cut(s) | 491 |
| HapII | CCGG | 1 cut(s) | 488 |
| Hin1II | CATG | 1 cut(s) | 359 |
| HincII | GTYRAC | 1 cut(s) | 434 |
| HindII | GTYRAC | 1 cut(s) | 434 |
| HinfI | GANTC | 3 cut(s) | 77, 203, 347 |
| HpaII | CCGG | 1 cut(s) | 488 |
| HphI | GGTGA | 2 cut(s) | 332, 458 |
| Hpy166II | GTNNAC | 1 cut(s) | 434 |
| Hpy188I | TCNGA | 1 cut(s) | 159 |
| Hpy188III | TCNNGA | 3 cut(s) | 128, 174, 200 |
| Hpy8I | GTNNAC | 1 cut(s) | 434 |
| HpyAV | CCTTC | 3 cut(s) | 329, 398, 479 |
| HpyCH4III | ACNGT | 1 cut(s) | 476 |
| HpyCH4V | TGCA | 1 cut(s) | 169 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 14 |
| HpyF3I | CTNAG | 2 cut(s) | 9, 273 |
| Hsp92II | CATG | 1 cut(s) | 359 |
| Ksp22I | TGATCA | 2 cut(s) | 67, 184 |
| Kzo9I | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| LpnPI | CCDG | 9 cut(s) | 4, 31, 123, 141, 159, 303, 364, 477, 501 |
| Lsp1109I | GCAGC | 2 cut(s) | 17, 281 |
| MaeIII | GTNAC | 2 cut(s) | 56, 256 |
| MalI | GATC | 7 cut(s) | 69, 145, 186, 364, 409, 486, 500 |
| MboI | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| MboII | GAAGA | 2 cut(s) | 303, 306 |
| MflI | RGATCY | 2 cut(s) | 143, 407 |
| MluCI | AATT | 5 cut(s) | 132, 215, 229, 416, 511 |
| MmeI | TCCRAC | 1 cut(s) | 262 |
| MnlI | CCTC | 4 cut(s) | 27, 134, 254, 281 |
| MseI | TTAA | 3 cut(s) | 218, 305, 514 |
| MslI | CAYNNNNRTG | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 1 cut(s) | 8 |
| MspI | CCGG | 1 cut(s) | 488 |
| MspR9I | CCNGG | 1 cut(s) | 19 |
| Mva1269I | GAATGC | 1 cut(s) | 19 |
| MvaI | CCWGG | 1 cut(s) | 19 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 14 |
| NdeII | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| NlaIII | CATG | 1 cut(s) | 359 |
| NmuCI | GTSAC | 2 cut(s) | 56, 256 |
| PctI | GAATGC | 1 cut(s) | 19 |
| PfeI | GAWTC | 3 cut(s) | 77, 203, 347 |
| PkrI | GCNGC | 2 cut(s) | 7, 271 |
| Psp6I | CCWGG | 1 cut(s) | 17 |
| PspGI | CCWGG | 1 cut(s) | 17 |
| PsuI | RGATCY | 2 cut(s) | 143, 407 |
| PvuII | CAGCTG | 1 cut(s) | 8 |
| RsaI | GTAC | 1 cut(s) | 460 |
| RsaNI | GTAC | 1 cut(s) | 459 |
| RseI | CAYNNNNRTG | 1 cut(s) | 263 |
| SaqAI | TTAA | 3 cut(s) | 218, 305, 514 |
| SatI | GCNGC | 2 cut(s) | 6, 270 |
| Sau3AI | GATC | 7 cut(s) | 67, 143, 184, 362, 407, 484, 498 |
| ScaI | AGTACT | 1 cut(s) | 460 |
| ScrFI | CCNGG | 1 cut(s) | 19 |
| SetI | ASST | 4 cut(s) | 10, 38, 246, 274 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 263 |
| Sse9I | AATT | 5 cut(s) | 132, 215, 229, 416, 511 |
| SspI | AATATT | 1 cut(s) | 120 |
| StyD4I | CCNGG | 1 cut(s) | 17 |
| TaaI | ACNGT | 1 cut(s) | 476 |
| TaqII | GACCGA | 1 cut(s) | 75 |
| TasI | AATT | 5 cut(s) | 132, 215, 229, 416, 511 |
| TatI | WGTACW | 1 cut(s) | 458 |
| TfiI | GAWTC | 3 cut(s) | 77, 203, 347 |
| Tru1I | TTAA | 3 cut(s) | 218, 305, 514 |
| Tru9I | TTAA | 3 cut(s) | 218, 305, 514 |
| TseFI | GTSAC | 2 cut(s) | 56, 256 |
| TseI | GCWGC | 2 cut(s) | 5, 269 |
| Tsp45I | GTSAC | 2 cut(s) | 56, 256 |
| TspDTI | ATGAA | 7 cut(s) | 113, 165, 177, 195, 237, 348, 429 |
| XapI | RAATTY | 1 cut(s) | 416 |
| ZrmI | AGTACT | 1 cut(s) | 460 |