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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 362 |
| AciI | CCGC | 2 cut(s) | 360, 516 |
| AclWI | GGATC | 2 cut(s) | 35, 113 |
| AcsI | RAATTY | 1 cut(s) | 653 |
| AfaI | GTAC | 2 cut(s) | 9, 133 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 121, 128 |
| AflIII | ACRYGT | 1 cut(s) | 59 |
| AgsI | TTSAA | 5 cut(s) | 77, 86, 275, 644, 658 |
| AjnI | CCWGG | 1 cut(s) | 323 |
| AluBI | AGCT | 2 cut(s) | 166, 194 |
| AluI | AGCT | 2 cut(s) | 166, 194 |
| Alw26I | GTCTC | 1 cut(s) | 518 |
| AlwI | GGATC | 2 cut(s) | 35, 113 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 518 |
| AoxI | GGCC | 1 cut(s) | 437 |
| ApeKI | GCWGC | 2 cut(s) | 194, 455 |
| ApoI | RAATTY | 1 cut(s) | 653 |
| AseI | ATTAAT | 1 cut(s) | 240 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 81 |
| AsuHPI | GGTGA | 2 cut(s) | 242, 445 |
| BbsI | GAAGAC | 1 cut(s) | 84 |
| BbvI | GCAGC | 2 cut(s) | 181, 442 |
| BciT130I | CCWGG | 1 cut(s) | 325 |
| BcoDI | GTCTC | 1 cut(s) | 518 |
| BfaI | CTAG | 1 cut(s) | 501 |
| BfmI | CTRYAG | 1 cut(s) | 528 |
| BisI | GCNGC | 3 cut(s) | 195, 456, 516 |
| BlsI | GCNGC | 3 cut(s) | 196, 457, 517 |
| Bme1390I | CCNGG | 1 cut(s) | 325 |
| BmiI | GGNNCC | 1 cut(s) | 319 |
| BmrFI | CCNGG | 1 cut(s) | 325 |
| BmsI | GCATC | 4 cut(s) | 333, 382, 403, 497 |
| BpiI | GAAGAC | 1 cut(s) | 84 |
| BpmI | CTGGAG | 2 cut(s) | 307, 333 |
| BpuEI | CTTGAG | 1 cut(s) | 426 |
| BsaJI | CCNNGG | 2 cut(s) | 324, 561 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 121, 128 |
| Bse1I | ACTGG | 1 cut(s) | 379 |
| BseBI | CCWGG | 1 cut(s) | 325 |
| BseDI | CCNNGG | 2 cut(s) | 324, 561 |
| BseGI | GGATG | 1 cut(s) | 348 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 121, 128 |
| BseNI | ACTGG | 1 cut(s) | 379 |
| BseXI | GCAGC | 2 cut(s) | 181, 442 |
| BshFI | GGCC | 1 cut(s) | 439 |
| BsiSI | CCGG | 1 cut(s) | 122 |
| BslFI | GGGAC | 1 cut(s) | 70 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 121, 128 |
| BsmAI | GTCTC | 1 cut(s) | 518 |
| BsmFI | GGGAC | 1 cut(s) | 70 |
| BsnI | GGCC | 1 cut(s) | 439 |
| Bsp143I | GATC | 3 cut(s) | 40, 118, 151 |
| BspACI | CCGC | 2 cut(s) | 360, 516 |
| BspANI | GGCC | 1 cut(s) | 439 |
| BspLI | GGNNCC | 1 cut(s) | 319 |
| BspPI | GGATC | 2 cut(s) | 35, 113 |
| BsrBI | CCGCTC | 1 cut(s) | 362 |
| BsrI | ACTGG | 1 cut(s) | 379 |
| BssECI | CCNNGG | 2 cut(s) | 324, 561 |
| BssMI | GATC | 3 cut(s) | 40, 118, 151 |
| BssT1I | CCWWGG | 1 cut(s) | 561 |
| Bst2UI | CCWGG | 1 cut(s) | 325 |
| Bst4CI | ACNGT | 1 cut(s) | 34 |
| Bst6I | CTCTTC | 1 cut(s) | 310 |
| BstC8I | GCNNGC | 1 cut(s) | 629 |
| BstF5I | GGATG | 1 cut(s) | 348 |
| BstKTI | GATC | 3 cut(s) | 43, 121, 154 |
| BstMAI | GTCTC | 1 cut(s) | 518 |
| BstMBI | GATC | 3 cut(s) | 40, 118, 151 |
| BstNI | CCWGG | 1 cut(s) | 325 |
| BstSCI | CCNGG | 1 cut(s) | 323 |
| BstSFI | CTRYAG | 1 cut(s) | 528 |
| BstV1I | GCAGC | 2 cut(s) | 181, 442 |
| BstV2I | GAAGAC | 1 cut(s) | 84 |
| BstX2I | RGATCY | 2 cut(s) | 40, 118 |
| BstYI | RGATCY | 2 cut(s) | 40, 118 |
| BsuRI | GGCC | 1 cut(s) | 439 |
| BtgZI | GCGATG | 1 cut(s) | 212 |
| BtsCI | GGATG | 1 cut(s) | 348 |
| Cac8I | GCNNGC | 1 cut(s) | 629 |
| CaiI | CAGNNNCTG | 1 cut(s) | 518 |
| Csp6I | GTAC | 2 cut(s) | 8, 132 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 167, 202 |
| CviAII | CATG | 1 cut(s) | 374 |
| CviJI | RGCY | 8 cut(s) | 107, 114, 166, 194, 311, 439, 455, 515 |
| CviKI_1 | RGCY | 8 cut(s) | 107, 114, 166, 194, 311, 439, 455, 515 |
| CviQI | GTAC | 2 cut(s) | 8, 132 |
| DpnI | GATC | 3 cut(s) | 42, 120, 153 |
| DpnII | GATC | 3 cut(s) | 40, 118, 151 |
| Eam1104I | CTCTTC | 1 cut(s) | 310 |
| EarI | CTCTTC | 1 cut(s) | 310 |
| Eco130I | CCWWGG | 1 cut(s) | 561 |
| Eco147I | AGGCCT | 1 cut(s) | 439 |
| EcoRI | GAATTC | 1 cut(s) | 653 |
| EcoRII | CCWGG | 1 cut(s) | 323 |
| EcoT14I | CCWWGG | 1 cut(s) | 561 |
| ErhI | CCWWGG | 1 cut(s) | 561 |
| FaeI | CATG | 1 cut(s) | 377 |
| FaiI | YATR | 7 cut(s) | 157, 375, 389, 530, 585, 621, 623 |
| FaqI | GGGAC | 1 cut(s) | 70 |
| FatI | CATG | 1 cut(s) | 373 |
| Fnu4HI | GCNGC | 3 cut(s) | 195, 456, 516 |
| FokI | GGATG | 1 cut(s) | 355 |
| Fsp4HI | GCNGC | 3 cut(s) | 195, 456, 516 |
| FspBI | CTAG | 1 cut(s) | 501 |
| GluI | GCNGC | 3 cut(s) | 195, 456, 516 |
| GsuI | CTGGAG | 2 cut(s) | 307, 333 |
| HaeIII | GGCC | 1 cut(s) | 439 |
| HapII | CCGG | 1 cut(s) | 122 |
| Hin1II | CATG | 1 cut(s) | 377 |
| HinfI | GANTC | 2 cut(s) | 368, 497 |
| HpaII | CCGG | 1 cut(s) | 122 |
| HphI | GGTGA | 2 cut(s) | 242, 445 |
| Hpy166II | GTNNAC | 2 cut(s) | 8, 406 |
| Hpy188I | TCNGA | 2 cut(s) | 474, 510 |
| Hpy188III | TCNNGA | 3 cut(s) | 365, 644, 665 |
| Hpy8I | GTNNAC | 2 cut(s) | 8, 406 |
| HpyAV | CCTTC | 2 cut(s) | 438, 445 |
| HpyCH4III | ACNGT | 1 cut(s) | 34 |
| HpyCH4IV | ACGT | 3 cut(s) | 61, 169, 571 |
| HpyCH4V | TGCA | 5 cut(s) | 93, 346, 395, 416, 631 |
| HpySE526I | ACGT | 3 cut(s) | 61, 169, 571 |
| Hsp92II | CATG | 1 cut(s) | 377 |
| Kzo9I | GATC | 3 cut(s) | 40, 118, 151 |
| LpnPI | CCDG | 5 cut(s) | 135, 297, 310, 337, 392 |
| Lsp1109I | GCAGC | 2 cut(s) | 181, 442 |
| LweI | GCATC | 4 cut(s) | 333, 382, 403, 497 |
| MaeI | CTAG | 1 cut(s) | 501 |
| MaeII | ACGT | 3 cut(s) | 61, 169, 571 |
| MaeIII | GTNAC | 2 cut(s) | 251, 599 |
| MalI | GATC | 3 cut(s) | 42, 120, 153 |
| MbiI | CCGCTC | 1 cut(s) | 362 |
| MboI | GATC | 3 cut(s) | 40, 118, 151 |
| MboII | GAAGA | 3 cut(s) | 89, 153, 297 |
| MfeI | CAATTG | 2 cut(s) | 86, 632 |
| MflI | RGATCY | 2 cut(s) | 40, 118 |
| MluCI | AATT | 5 cut(s) | 86, 241, 278, 632, 653 |
| MmeI | TCCRAC | 1 cut(s) | 363 |
| MroXI | GAANNNNTTC | 1 cut(s) | 81 |
| MseI | TTAA | 2 cut(s) | 240, 264 |
| MslI | CAYNNNNRTG | 1 cut(s) | 597 |
| MspA1I | CMGCKG | 1 cut(s) | 518 |
| MspI | CCGG | 1 cut(s) | 122 |
| MspR9I | CCNGG | 1 cut(s) | 325 |
| MunI | CAATTG | 2 cut(s) | 86, 632 |
| MvaI | CCWGG | 1 cut(s) | 325 |
| NdeII | GATC | 3 cut(s) | 40, 118, 151 |
| NlaIII | CATG | 1 cut(s) | 377 |
| NlaIV | GGNNCC | 1 cut(s) | 319 |
| NmuCI | GTSAC | 1 cut(s) | 599 |
| PceI | AGGCCT | 1 cut(s) | 439 |
| PdmI | GAANNNNTTC | 1 cut(s) | 81 |
| PfeI | GAWTC | 2 cut(s) | 368, 497 |
| PkrI | GCNGC | 3 cut(s) | 196, 457, 517 |
| PshBI | ATTAAT | 1 cut(s) | 240 |
| Psp6I | CCWGG | 1 cut(s) | 323 |
| PspGI | CCWGG | 1 cut(s) | 323 |
| PspN4I | GGNNCC | 1 cut(s) | 319 |
| PstNI | CAGNNNCTG | 1 cut(s) | 518 |
| PsuI | RGATCY | 2 cut(s) | 40, 118 |
| RsaI | GTAC | 2 cut(s) | 9, 133 |
| RsaNI | GTAC | 2 cut(s) | 8, 132 |
| RseI | CAYNNNNRTG | 1 cut(s) | 597 |
| SaqAI | TTAA | 2 cut(s) | 240, 264 |
| SatI | GCNGC | 3 cut(s) | 195, 456, 516 |
| Sau3AI | GATC | 3 cut(s) | 40, 118, 151 |
| ScrFI | CCNGG | 1 cut(s) | 325 |
| SetI | ASST | 8 cut(s) | 64, 168, 172, 196, 232, 323, 405, 574 |
| SfaNI | GCATC | 4 cut(s) | 333, 382, 403, 497 |
| SfcI | CTRYAG | 1 cut(s) | 528 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 597 |
| SmlI | CTYRAG | 1 cut(s) | 441 |
| SmoI | CTYRAG | 1 cut(s) | 441 |
| Sse9I | AATT | 5 cut(s) | 86, 241, 278, 632, 653 |
| SseBI | AGGCCT | 1 cut(s) | 439 |
| SsiI | CCGC | 2 cut(s) | 360, 516 |
| SspMI | CTAG | 1 cut(s) | 501 |
| StuI | AGGCCT | 1 cut(s) | 439 |
| StyD4I | CCNGG | 1 cut(s) | 323 |
| StyI | CCWWGG | 1 cut(s) | 561 |
| TaaI | ACNGT | 1 cut(s) | 34 |
| TaiI | ACGT | 3 cut(s) | 64, 172, 574 |
| TaqI | TCGA | 1 cut(s) | 589 |
| TasI | AATT | 5 cut(s) | 86, 241, 278, 632, 653 |
| TauI | GCSGC | 1 cut(s) | 518 |
| TfiI | GAWTC | 2 cut(s) | 368, 497 |
| Tru1I | TTAA | 2 cut(s) | 240, 264 |
| Tru9I | TTAA | 2 cut(s) | 240, 264 |
| TseFI | GTSAC | 1 cut(s) | 599 |
| TseI | GCWGC | 2 cut(s) | 194, 455 |
| Tsp45I | GTSAC | 1 cut(s) | 599 |
| TspDTI | ATGAA | 1 cut(s) | 226 |
| TspGWI | ACGGA | 1 cut(s) | 149 |
| VspI | ATTAAT | 1 cut(s) | 240 |
| XapI | RAATTY | 1 cut(s) | 653 |
| XmnI | GAANNNNTTC | 1 cut(s) | 81 |
| XspI | CTAG | 1 cut(s) | 501 |