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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 141 |
| AciI | CCGC | 2 cut(s) | 141, 152 |
| AcsI | RAATTY | 1 cut(s) | 75 |
| AfaI | GTAC | 1 cut(s) | 134 |
| AgsI | TTSAA | 2 cut(s) | 82, 89 |
| AluBI | AGCT | 1 cut(s) | 50 |
| AluI | AGCT | 1 cut(s) | 50 |
| AoxI | GGCC | 1 cut(s) | 32 |
| ApoI | RAATTY | 1 cut(s) | 75 |
| AspS9I | GGNCC | 1 cut(s) | 33 |
| AsuII | TTCGAA | 1 cut(s) | 146 |
| BmgT120I | GGNCC | 1 cut(s) | 33 |
| BmiI | GGNNCC | 1 cut(s) | 35 |
| Bpu14I | TTCGAA | 1 cut(s) | 146 |
| BshFI | GGCC | 1 cut(s) | 34 |
| BsmI | GAATGC | 1 cut(s) | 154 |
| BsnI | GGCC | 1 cut(s) | 34 |
| Bsp119I | TTCGAA | 1 cut(s) | 146 |
| Bsp143I | GATC | 1 cut(s) | 44 |
| BspACI | CCGC | 2 cut(s) | 141, 152 |
| BspANI | GGCC | 1 cut(s) | 34 |
| BspLI | GGNNCC | 1 cut(s) | 35 |
| BspT104I | TTCGAA | 1 cut(s) | 146 |
| BsrBI | CCGCTC | 1 cut(s) | 141 |
| BssMI | GATC | 1 cut(s) | 44 |
| BstBI | TTCGAA | 1 cut(s) | 146 |
| BstC8I | GCNNGC | 1 cut(s) | 120 |
| BstKTI | GATC | 1 cut(s) | 47 |
| BstMBI | GATC | 1 cut(s) | 44 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 10 |
| BsuRI | GGCC | 1 cut(s) | 34 |
| Cac8I | GCNNGC | 1 cut(s) | 120 |
| Cfr13I | GGNCC | 1 cut(s) | 33 |
| Csp6I | GTAC | 1 cut(s) | 133 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 50, 85 |
| CviJI | RGCY | 3 cut(s) | 34, 42, 50 |
| CviKI_1 | RGCY | 3 cut(s) | 34, 42, 50 |
| CviQI | GTAC | 1 cut(s) | 133 |
| DpnI | GATC | 1 cut(s) | 46 |
| DpnII | GATC | 1 cut(s) | 44 |
| FaiI | YATR | 2 cut(s) | 96, 166 |
| HaeIII | GGCC | 1 cut(s) | 34 |
| HpyAV | CCTTC | 2 cut(s) | 60, 76 |
| HpyCH4V | TGCA | 2 cut(s) | 61, 110 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 10 |
| Kzo9I | GATC | 1 cut(s) | 44 |
| LpnPI | CCDG | 1 cut(s) | 50 |
| MaeIII | GTNAC | 1 cut(s) | 22 |
| MalI | GATC | 1 cut(s) | 46 |
| MbiI | CCGCTC | 1 cut(s) | 141 |
| MboI | GATC | 1 cut(s) | 44 |
| MluCI | AATT | 4 cut(s) | 17, 28, 75, 127 |
| MnlI | CCTC | 3 cut(s) | 3, 64, 154 |
| MseI | TTAA | 1 cut(s) | 113 |
| Mva1269I | GAATGC | 1 cut(s) | 154 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 10 |
| NdeII | GATC | 1 cut(s) | 44 |
| NlaIV | GGNNCC | 1 cut(s) | 35 |
| NspV | TTCGAA | 1 cut(s) | 146 |
| PctI | GAATGC | 1 cut(s) | 154 |
| PspN4I | GGNNCC | 1 cut(s) | 35 |
| PspPI | GGNCC | 1 cut(s) | 33 |
| RsaI | GTAC | 1 cut(s) | 134 |
| RsaNI | GTAC | 1 cut(s) | 133 |
| SaqAI | TTAA | 1 cut(s) | 113 |
| Sau3AI | GATC | 1 cut(s) | 44 |
| Sau96I | GGNCC | 1 cut(s) | 33 |
| SetI | ASST | 4 cut(s) | 52, 71, 87, 165 |
| SfuI | TTCGAA | 1 cut(s) | 146 |
| SgeI | CNNG | 5 cut(s) | 17, 49, 74, 131, 148 |
| Sse9I | AATT | 4 cut(s) | 17, 28, 75, 127 |
| SsiI | CCGC | 2 cut(s) | 141, 152 |
| TaqI | TCGA | 1 cut(s) | 146 |
| TasI | AATT | 4 cut(s) | 17, 28, 75, 127 |
| Tru1I | TTAA | 1 cut(s) | 113 |
| Tru9I | TTAA | 1 cut(s) | 113 |
| XapI | RAATTY | 1 cut(s) | 75 |