No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 167 |
| Acc16I | TGCGCA | 1 cut(s) | 113 |
| Acc36I | ACCTGC | 1 cut(s) | 167 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 128, 527 |
| AccIII | TCCGGA | 1 cut(s) | 77 |
| AciI | CCGC | 3 cut(s) | 321, 434, 488 |
| AclWI | GGATC | 2 cut(s) | 185, 580 |
| AcsI | RAATTY | 3 cut(s) | 133, 347, 523 |
| AcyI | GRCGYC | 1 cut(s) | 461 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 128, 313, 473, 500, 527, 557 |
| Alw26I | GTCTC | 1 cut(s) | 365 |
| AlwI | GGATC | 2 cut(s) | 185, 580 |
| Aor13HI | TCCGGA | 1 cut(s) | 77 |
| AoxI | GGCC | 1 cut(s) | 203 |
| ApeKI | GCWGC | 1 cut(s) | 254 |
| ApoI | RAATTY | 3 cut(s) | 133, 347, 523 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 234 |
| AspLEI | GCGC | 1 cut(s) | 114 |
| AsuC2I | CCSGG | 1 cut(s) | 558 |
| AsuHPI | GGTGA | 3 cut(s) | 191, 464, 515 |
| BbvI | GCAGC | 1 cut(s) | 266 |
| BccI | CCATC | 1 cut(s) | 149 |
| BciVI | GTATCC | 1 cut(s) | 473 |
| BcnI | CCSGG | 1 cut(s) | 558 |
| BcoDI | GTCTC | 1 cut(s) | 365 |
| BfaI | CTAG | 1 cut(s) | 21 |
| BfmI | CTRYAG | 1 cut(s) | 186 |
| BfuAI | ACCTGC | 1 cut(s) | 167 |
| BfuI | GTATCC | 1 cut(s) | 473 |
| BglII | AGATCT | 1 cut(s) | 445 |
| BisI | GCNGC | 1 cut(s) | 255 |
| BlsI | GCNGC | 1 cut(s) | 256 |
| Bme1390I | CCNGG | 1 cut(s) | 558 |
| BmrFI | CCNGG | 1 cut(s) | 558 |
| BmsI | GCATC | 1 cut(s) | 496 |
| BpuEI | CTTGAG | 2 cut(s) | 192, 272 |
| BpuMI | CCSGG | 1 cut(s) | 558 |
| BsaHI | GRCGYC | 1 cut(s) | 461 |
| BsaJI | CCNNGG | 1 cut(s) | 556 |
| BsaWI | WCCGGW | 1 cut(s) | 77 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 128, 313, 473, 500, 527, 557 |
| BseAI | TCCGGA | 1 cut(s) | 77 |
| BseDI | CCNNGG | 1 cut(s) | 556 |
| BseGI | GGATG | 3 cut(s) | 60, 74, 163 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 128, 313, 473, 500, 527, 557 |
| BseXI | GCAGC | 1 cut(s) | 266 |
| BshFI | GGCC | 1 cut(s) | 205 |
| BsiSI | CCGG | 2 cut(s) | 78, 558 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 128, 313, 473, 500, 527, 557 |
| BsmAI | GTCTC | 1 cut(s) | 365 |
| BsmI | GAATGC | 1 cut(s) | 509 |
| BsnI | GGCC | 1 cut(s) | 205 |
| Bsp13I | TCCGGA | 1 cut(s) | 77 |
| Bsp143I | GATC | 4 cut(s) | 190, 262, 445, 585 |
| BspACI | CCGC | 3 cut(s) | 321, 434, 488 |
| BspANI | GGCC | 1 cut(s) | 205 |
| BspEI | TCCGGA | 1 cut(s) | 77 |
| BspMAI | CTGCAG | 1 cut(s) | 190 |
| BspMI | ACCTGC | 1 cut(s) | 167 |
| BspPI | GGATC | 2 cut(s) | 185, 580 |
| BssECI | CCNNGG | 1 cut(s) | 556 |
| BssMI | GATC | 4 cut(s) | 190, 262, 445, 585 |
| BssNI | GRCGYC | 1 cut(s) | 461 |
| Bst4CI | ACNGT | 1 cut(s) | 14 |
| Bst6I | CTCTTC | 1 cut(s) | 366 |
| BstACI | GRCGYC | 1 cut(s) | 461 |
| BstC8I | GCNNGC | 1 cut(s) | 325 |
| BstF5I | GGATG | 3 cut(s) | 60, 74, 163 |
| BstHHI | GCGC | 1 cut(s) | 114 |
| BstKTI | GATC | 4 cut(s) | 193, 265, 448, 588 |
| BstMAI | GTCTC | 1 cut(s) | 365 |
| BstMBI | GATC | 4 cut(s) | 190, 262, 445, 585 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 26 |
| BstNSI | RCATGY | 1 cut(s) | 387 |
| BstSCI | CCNGG | 1 cut(s) | 556 |
| BstSFI | CTRYAG | 1 cut(s) | 186 |
| BstV1I | GCAGC | 1 cut(s) | 266 |
| BstX2I | RGATCY | 2 cut(s) | 190, 445 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 148 |
| BstYI | RGATCY | 2 cut(s) | 190, 445 |
| BsuI | GTATCC | 1 cut(s) | 473 |
| BsuRI | GGCC | 1 cut(s) | 205 |
| BtsCI | GGATG | 3 cut(s) | 60, 74, 163 |
| BveI | ACCTGC | 1 cut(s) | 167 |
| Cac8I | GCNNGC | 1 cut(s) | 325 |
| CfoI | GCGC | 1 cut(s) | 114 |
| CseI | GACGC | 1 cut(s) | 469 |
| CviAII | CATG | 2 cut(s) | 154, 384 |
| CviJI | RGCY | 2 cut(s) | 205, 221 |
| CviKI_1 | RGCY | 2 cut(s) | 205, 221 |
| DpnI | GATC | 4 cut(s) | 192, 264, 447, 587 |
| DpnII | GATC | 4 cut(s) | 190, 262, 445, 585 |
| DraI | TTTAAA | 1 cut(s) | 55 |
| Eam1104I | CTCTTC | 1 cut(s) | 366 |
| EarI | CTCTTC | 1 cut(s) | 366 |
| Eco147I | AGGCCT | 1 cut(s) | 205 |
| EcoRI | GAATTC | 1 cut(s) | 347 |
| EcoT22I | ATGCAT | 2 cut(s) | 6, 511 |
| FaeI | CATG | 2 cut(s) | 157, 387 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 432, 464 |
| FatI | CATG | 2 cut(s) | 153, 383 |
| FauNDI | CATATG | 1 cut(s) | 108 |
| Fnu4HI | GCNGC | 1 cut(s) | 255 |
| FokI | GGATG | 3 cut(s) | 47, 61, 170 |
| Fsp4HI | GCNGC | 1 cut(s) | 255 |
| FspAI | RTGCGCAY | 1 cut(s) | 113 |
| FspBI | CTAG | 1 cut(s) | 21 |
| FspI | TGCGCA | 1 cut(s) | 113 |
| GlaI | GCGC | 1 cut(s) | 113 |
| GluI | GCNGC | 1 cut(s) | 255 |
| HaeIII | GGCC | 1 cut(s) | 205 |
| HapII | CCGG | 2 cut(s) | 78, 558 |
| HgaI | GACGC | 1 cut(s) | 469 |
| HhaI | GCGC | 1 cut(s) | 114 |
| Hin1I | GRCGYC | 1 cut(s) | 461 |
| Hin1II | CATG | 2 cut(s) | 157, 387 |
| Hin6I | GCGC | 1 cut(s) | 112 |
| HinP1I | GCGC | 1 cut(s) | 112 |
| HpaII | CCGG | 2 cut(s) | 78, 558 |
| HphI | GGTGA | 3 cut(s) | 191, 464, 515 |
| Hpy188I | TCNGA | 1 cut(s) | 240 |
| Hpy188III | TCNNGA | 2 cut(s) | 78, 289 |
| HpyAV | CCTTC | 5 cut(s) | 89, 207, 211, 244, 477 |
| HpyCH4III | ACNGT | 1 cut(s) | 14 |
| HpyCH4IV | ACGT | 1 cut(s) | 198 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 47, 176, 188, 327, 397, 509, 536, 563 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 26 |
| HpySE526I | ACGT | 1 cut(s) | 198 |
| Hsp92I | GRCGYC | 1 cut(s) | 461 |
| Hsp92II | CATG | 2 cut(s) | 157, 387 |
| HspAI | GCGC | 1 cut(s) | 112 |
| Kpn2I | TCCGGA | 1 cut(s) | 77 |
| Kzo9I | GATC | 4 cut(s) | 190, 262, 445, 585 |
| LmnI | GCTCC | 1 cut(s) | 436 |
| LpnPI | CCDG | 5 cut(s) | 91, 162, 187, 571, 591 |
| Lsp1109I | GCAGC | 1 cut(s) | 266 |
| LweI | GCATC | 1 cut(s) | 496 |
| MaeI | CTAG | 1 cut(s) | 21 |
| MaeII | ACGT | 1 cut(s) | 198 |
| MaeIII | GTNAC | 1 cut(s) | 23 |
| MalI | GATC | 4 cut(s) | 192, 264, 447, 587 |
| MboI | GATC | 4 cut(s) | 190, 262, 445, 585 |
| MboII | GAAGA | 4 cut(s) | 382, 383, 439, 455 |
| MflI | RGATCY | 2 cut(s) | 190, 445 |
| MluCI | AATT | 7 cut(s) | 123, 133, 171, 234, 347, 496, 523 |
| MnlI | CCTC | 6 cut(s) | 31, 74, 160, 216, 307, 361 |
| Mph1103I | ATGCAT | 2 cut(s) | 6, 511 |
| MroI | TCCGGA | 1 cut(s) | 77 |
| MroXI | GAANNNNTTC | 1 cut(s) | 234 |
| MseI | TTAA | 3 cut(s) | 54, 89, 607 |
| MspI | CCGG | 2 cut(s) | 78, 558 |
| MspR9I | CCNGG | 1 cut(s) | 558 |
| Mva1269I | GAATGC | 1 cut(s) | 509 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 26 |
| NciI | CCSGG | 1 cut(s) | 558 |
| NdeI | CATATG | 1 cut(s) | 108 |
| NdeII | GATC | 4 cut(s) | 190, 262, 445, 585 |
| NlaIII | CATG | 2 cut(s) | 157, 387 |
| NsbI | TGCGCA | 1 cut(s) | 113 |
| NsiI | ATGCAT | 2 cut(s) | 6, 511 |
| NspI | RCATGY | 1 cut(s) | 387 |
| PaqCI | CACCTGC | 1 cut(s) | 167 |
| PceI | AGGCCT | 1 cut(s) | 205 |
| PctI | GAATGC | 1 cut(s) | 509 |
| PdmI | GAANNNNTTC | 1 cut(s) | 234 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 128, 527 |
| PkrI | GCNGC | 1 cut(s) | 256 |
| PstI | CTGCAG | 1 cut(s) | 190 |
| PsuI | RGATCY | 2 cut(s) | 190, 445 |
| SaqAI | TTAA | 3 cut(s) | 54, 89, 607 |
| SatI | GCNGC | 1 cut(s) | 255 |
| Sau3AI | GATC | 4 cut(s) | 190, 262, 445, 585 |
| ScrFI | CCNGG | 1 cut(s) | 558 |
| SetI | ASST | 6 cut(s) | 171, 181, 201, 469, 553, 603 |
| SfaNI | GCATC | 1 cut(s) | 496 |
| SfcI | CTRYAG | 1 cut(s) | 186 |
| SmlI | CTYRAG | 2 cut(s) | 207, 287 |
| SmoI | CTYRAG | 2 cut(s) | 207, 287 |
| Sse9I | AATT | 7 cut(s) | 123, 133, 171, 234, 347, 496, 523 |
| SseBI | AGGCCT | 1 cut(s) | 205 |
| SsiI | CCGC | 3 cut(s) | 321, 434, 488 |
| SspMI | CTAG | 1 cut(s) | 21 |
| StuI | AGGCCT | 1 cut(s) | 205 |
| StyD4I | CCNGG | 1 cut(s) | 556 |
| TaaI | ACNGT | 1 cut(s) | 14 |
| TaiI | ACGT | 1 cut(s) | 201 |
| TaqI | TCGA | 3 cut(s) | 339, 355, 402 |
| TasI | AATT | 7 cut(s) | 123, 133, 171, 234, 347, 496, 523 |
| Tru1I | TTAA | 3 cut(s) | 54, 89, 607 |
| Tru9I | TTAA | 3 cut(s) | 54, 89, 607 |
| TseI | GCWGC | 1 cut(s) | 254 |
| TspDTI | ATGAA | 1 cut(s) | 108 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 128, 527 |
| XapI | RAATTY | 3 cut(s) | 133, 347, 523 |
| XceI | RCATGY | 1 cut(s) | 387 |
| XmnI | GAANNNNTTC | 1 cut(s) | 234 |
| XspI | CTAG | 1 cut(s) | 21 |
| Zsp2I | ATGCAT | 2 cut(s) | 6, 511 |