FvH4_6g21872

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001009
Physical Location & Seq
Forward (+)
15548382 .. 15549761
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21872.t1

Sequence Viewer

Length: 1236 bp
ATGCCAAATACTTGGGATGTTACTGTCAACTCTATTTGCAGTTCAGCTGGGAAAAACAAGTTGGTCTACAACGAAGTTCGGGATACCCTGTTGAGTGAGGAAACTAAGAGGAAGAAGAATGATGATGAAGAGTCATCTGCGACTACATCTACTTTGCTTGTAAGAGGAAGATCTAGTGTAAAGGAGGGTACAAATCGTGGTAGATCAAAGTCAAGGAGTTTTAGAGATCAAGATAGAGACAAAGATAAGGAGTGCTTTTACTGTCATGATTTTGGGCATATTAGGTATAATTGTCTAAAATTGAAGTTGAAGAGAGCTACTACTGCAATGGTAGCAAGTGAAGATGATGGAGAATGTTATGCAGATGCGCTCATGGTCACATCATGTTTAAAGACGCCACATGATGAGTGGATTTTGGATACTAGTTGTTTCGAGCACATGTGTCCCAATAAGGATTGGTTTTCTTCCTACAAGTCTGTGAAGGGAAGTCTAGTGAGGTCGGTTGGTGCACAAACCTACAAAGTTGTTGGTGTTGGAACAATCAAAATTAAGGTACATGATGGATCAGTGAGGAGTTTAGAGAATGTTCTCCATGTTCCTAAGTTCAATAGGAATCTGATATCTTTGGGAACTTTAGATTCGGAAGGCCACAAGATTGTTGGTCAAGATGGACATATGAAAGTTTTGAAGGGGGCACTCGTAGTAATGAAAGGTGGAAAGATTGACAATCTTGGTTCCTATTGGCATTCAAGGGAGATGAGCAATAGTAAATGCGAACGAACGAAATCAGCTTCTAAGAAGGTGAAATTTGAGGAGCAACCAACATTGCAGCAGTCCTTAAAGAGAGGCAGACCCATAAGGATCCCGACACAAGTTTGTGGTGGAGATATGCTAGTTCATGCATATTCCACAATTGAGGTGTCACCTACTTCAAAGAGTAGTGAGAAAGTTGAGAAACAAACTTGGTGTTTTAAGAGTGATGCGTCTAGAGAAGATTTCGTTCATGGTGGAGAATATGTTAAGAGAAATGTTGCTTGTGATGAGTTGTGCAAACCATTGAAGATCGCTCCCATTAGGTTTAGCAACTTTAGTGGAGACTTGGATTGCATGCAAGTGGCTGCGCTGTGCATGAGGGAAAGTGTTCGTGTCCAAGGGTCAAGTTTGAGTGTTTTTCAAACTTGGCTAACTCAGCTTGCTGTTGAGGGCGCCATGTGGCGCATTGAGGTGTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

412

Amino Acids

46.28

Weight (kDa)

8.96

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 137 - 216 6.4e-27 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1205
AccI GTMKAC 1 cut(s) 66
AclWI GGATC 3 cut(s) 571, 856, 869
AcsI RAATTY 1 cut(s) 806
AcyI GRCGYC 2 cut(s) 395, 1206
AfaI GTAC 2 cut(s) 190, 555
AflIII ACRYGT 1 cut(s) 438
AgsI TTSAA 8 cut(s) 304, 310, 607, 688, 750, 933, 1060, 1175
AhlI ACTAGT 1 cut(s) 422
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 4 cut(s) 47, 317, 791, 1192
AluI AGCT 4 cut(s) 47, 317, 791, 1192
Alw21I GWGCWC 2 cut(s) 438, 511
Alw26I GTCTC 2 cut(s) 231, 1089
Alw44I GTGCAC 1 cut(s) 507
AlwI GGATC 3 cut(s) 571, 856, 869
AoxI GGCC 1 cut(s) 646
ApaLI GTGCAC 1 cut(s) 507
ApeKI GCWGC 2 cut(s) 829, 1118
ApoI RAATTY 1 cut(s) 806
Asp700I GAANNNNTTC 1 cut(s) 1140
AspLEI GCGC 4 cut(s) 370, 1123, 1208, 1218
AsuHPI GGTGA 2 cut(s) 814, 915
BaeGI GKGCMC 2 cut(s) 511, 697
BamHI GGATCC 1 cut(s) 861
BanI GGYRCC 1 cut(s) 1205
Bbv12I GWGCWC 2 cut(s) 438, 511
BbvI GCAGC 2 cut(s) 841, 1105
BccI CCATC 3 cut(s) 341, 554, 662
BciVI GTATCC 2 cut(s) 76, 412
BcoDI GTCTC 2 cut(s) 231, 1089
BcuI ACTAGT 1 cut(s) 422
BfaI CTAG 5 cut(s) 174, 423, 491, 893, 987
BfoI RGCGCY 1 cut(s) 1209
BfuI GTATCC 2 cut(s) 76, 412
BglII AGATCT 1 cut(s) 170
BisI GCNGC 2 cut(s) 830, 1119
BlsI GCNGC 2 cut(s) 831, 1120
BmiI GGNNCC 3 cut(s) 736, 863, 1207
BmsI GCATC 2 cut(s) 355, 970
BsaHI GRCGYC 2 cut(s) 395, 1206
BsaJI CCNNGG 1 cut(s) 1150
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bse3DI GCAATG 2 cut(s) 333, 824
BseDI CCNNGG 1 cut(s) 1150
BseGI GGATG 1 cut(s) 22
BseMI GCAATG 2 cut(s) 333, 824
BseMII CTCAG 1 cut(s) 1202
BseRI GAGGAG 2 cut(s) 586, 827
BseSI GKGCMC 2 cut(s) 511, 697
BseXI GCAGC 2 cut(s) 841, 1105
BseYI CCCAGC 1 cut(s) 47
BshFI GGCC 1 cut(s) 648
BshNI GGYRCC 1 cut(s) 1205
BsiHKAI GWGCWC 2 cut(s) 438, 511
BslFI GGGAC 1 cut(s) 429
BsmAI GTCTC 2 cut(s) 231, 1089
BsmFI GGGAC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 1 cut(s) 648
Bsp1286I GDGCHC 3 cut(s) 438, 511, 697
Bsp143I GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
BspANI GGCC 1 cut(s) 648
BspCNI CTCAG 1 cut(s) 1201
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 736, 863, 1207
BspPI GGATC 3 cut(s) 571, 856, 869
BspT107I GGYRCC 1 cut(s) 1205
BsrDI GCAATG 2 cut(s) 333, 824
BssECI CCNNGG 1 cut(s) 1150
BssMI GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
BssNI GRCGYC 2 cut(s) 395, 1206
BssT1I CCWWGG 1 cut(s) 1150
Bst4CI ACNGT 2 cut(s) 25, 263
Bst6I CTCTTC 2 cut(s) 123, 305
BstACI GRCGYC 2 cut(s) 395, 1206
BstC8I GCNNGC 2 cut(s) 1109, 1194
BstDEI CTNAG 4 cut(s) 105, 600, 795, 1188
BstF5I GGATG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 1209
BstHHI GCGC 4 cut(s) 370, 1123, 1208, 1218
BstKTI GATC 6 cut(s) 173, 206, 229, 566, 864, 1065
BstMAI GTCTC 2 cut(s) 231, 1089
BstMBI GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
BstMWI GCNNNNNNNGC 3 cut(s) 323, 332, 1189
BstNSI RCATGY 2 cut(s) 442, 1111
BstSLI GKGCMC 2 cut(s) 511, 697
BstV1I GCAGC 2 cut(s) 841, 1105
BstX2I RGATCY 2 cut(s) 170, 861
BstXI CCANNNNNNTGG 1 cut(s) 12
BstYI RGATCY 2 cut(s) 170, 861
BsuI GTATCC 2 cut(s) 76, 412
BsuRI GGCC 1 cut(s) 648
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 2 cut(s) 1109, 1194
CciI TCATGA 1 cut(s) 265
CfoI GCGC 4 cut(s) 370, 1123, 1208, 1218
CseI GACGC 2 cut(s) 403, 972
Csp6I GTAC 2 cut(s) 189, 554
CspCI CAANNNNNGTGG 2 cut(s) 1072, 1107
CviJI RGCY 7 cut(s) 47, 317, 648, 791, 1118, 1183, 1192
CviKI_1 RGCY 7 cut(s) 47, 317, 648, 791, 1118, 1183, 1192
CviQI GTAC 2 cut(s) 189, 554
DdeI CTNAG 4 cut(s) 105, 600, 795, 1188
DinI GGCGCC 1 cut(s) 1207
DpnI GATC 6 cut(s) 172, 205, 228, 565, 863, 1064
DpnII GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
DraI TTTAAA 1 cut(s) 390
Eam1104I CTCTTC 2 cut(s) 123, 305
EarI CTCTTC 2 cut(s) 123, 305
Eco130I CCWWGG 1 cut(s) 1150
Eco32I GATATC 1 cut(s) 621
EcoRV GATATC 1 cut(s) 621
EcoT14I CCWWGG 1 cut(s) 1150
EcoT22I ATGCAT 1 cut(s) 904
EgeI GGCGCC 1 cut(s) 1207
EheI GGCGCC 1 cut(s) 1207
ErhI CCWWGG 1 cut(s) 1150
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 1 cut(s) 429
FauNDI CATATG 1 cut(s) 675
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 830, 1119
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 830, 1119
FspBI CTAG 5 cut(s) 174, 423, 491, 893, 987
GlaI GCGC 4 cut(s) 369, 1122, 1207, 1217
GluI GCNGC 2 cut(s) 830, 1119
GsaI CCCAGC 1 cut(s) 51
HaeII RGCGCY 1 cut(s) 1209
HaeIII GGCC 1 cut(s) 648
HgaI GACGC 2 cut(s) 403, 972
HhaI GCGC 4 cut(s) 370, 1123, 1208, 1218
Hin1I GRCGYC 2 cut(s) 395, 1206
Hin6I GCGC 4 cut(s) 368, 1121, 1206, 1216
HinP1I GCGC 4 cut(s) 368, 1121, 1206, 1216
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 3 cut(s) 131, 613, 638
HphI GGTGA 2 cut(s) 814, 915
Hpy166II GTNNAC 3 cut(s) 28, 67, 509
Hpy188I TCNGA 2 cut(s) 618, 643
Hpy188III TCNNGA 6 cut(s) 80, 230, 266, 665, 865, 987
Hpy8I GTNNAC 3 cut(s) 28, 67, 509
HpyAV CCTTC 4 cut(s) 475, 638, 682, 793
HpyCH4III ACNGT 2 cut(s) 25, 263
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 332, 1189
HpyF3I CTNAG 4 cut(s) 105, 600, 795, 1188
Hsp92I GRCGYC 2 cut(s) 395, 1206
HspAI GCGC 4 cut(s) 368, 1121, 1206, 1216
KasI GGCGCC 1 cut(s) 1205
Kzo9I GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
LmnI GCTCC 2 cut(s) 814, 1072
LpnPI CCDG 2 cut(s) 33, 101
Lsp1109I GCAGC 2 cut(s) 841, 1105
LweI GCATC 2 cut(s) 355, 970
MaeI CTAG 5 cut(s) 174, 423, 491, 893, 987
MaeIII GTNAC 3 cut(s) 19, 376, 921
MalI GATC 6 cut(s) 172, 205, 228, 565, 863, 1064
MboI GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
MboII GAAGA 9 cut(s) 124, 127, 140, 180, 322, 353, 456, 1004, 1072
MfeI CAATTG 1 cut(s) 912
MflI RGATCY 2 cut(s) 170, 861
MhlI GDGCHC 3 cut(s) 438, 511, 697
MluCI AATT 5 cut(s) 289, 299, 546, 806, 912
Mly113I GGCGCC 1 cut(s) 1206
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 514
Mph1103I ATGCAT 1 cut(s) 904
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 5 cut(s) 389, 549, 839, 972, 1020
MslI CAYNNNNRTG 2 cut(s) 1112, 1223
MspA1I CMGCKG 1 cut(s) 47
MunI CAATTG 1 cut(s) 912
Mva1269I GAATGC 1 cut(s) 745
MwoI GCNNNNNNNGC 3 cut(s) 323, 332, 1189
NarI GGCGCC 1 cut(s) 1206
NdeI CATATG 1 cut(s) 675
NdeII GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
NlaIV GGNNCC 3 cut(s) 736, 863, 1207
NmuCI GTSAC 2 cut(s) 376, 921
NsiI ATGCAT 1 cut(s) 904
NspI RCATGY 2 cut(s) 442, 1111
PaeI GCATGC 1 cut(s) 1111
PagI TCATGA 1 cut(s) 265
PciI ACATGT 1 cut(s) 438
PctI GAATGC 1 cut(s) 745
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 2 cut(s) 613, 638
PkrI GCNGC 2 cut(s) 831, 1120
PleI GAGTC 1 cut(s) 139
PluTI GGCGCC 1 cut(s) 1209
PpsI GAGTC 1 cut(s) 139
PscI ACATGT 1 cut(s) 438
PspFI CCCAGC 1 cut(s) 47
PspN4I GGNNCC 3 cut(s) 736, 863, 1207
PsuI RGATCY 2 cut(s) 170, 861
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 2 cut(s) 190, 555
RsaNI GTAC 2 cut(s) 189, 554
RseI CAYNNNNRTG 2 cut(s) 1112, 1223
SaqAI TTAA 5 cut(s) 389, 549, 839, 972, 1020
SatI GCNGC 2 cut(s) 830, 1119
Sau3AI GATC 6 cut(s) 170, 203, 226, 563, 861, 1062
SchI GAGTC 1 cut(s) 140
SduI GDGCHC 3 cut(s) 438, 511, 697
SfaNI GCATC 2 cut(s) 355, 970
SfoI GGCGCC 1 cut(s) 1207
SmiMI CAYNNNNRTG 2 cut(s) 1112, 1223
SpeI ACTAGT 1 cut(s) 422
SphI GCATGC 1 cut(s) 1111
Sse9I AATT 5 cut(s) 289, 299, 546, 806, 912
SspDI GGCGCC 1 cut(s) 1205
SspMI CTAG 5 cut(s) 174, 423, 491, 893, 987
StyI CCWWGG 1 cut(s) 1150
TaaI ACNGT 2 cut(s) 25, 263
TaqI TCGA 1 cut(s) 432
TasI AATT 5 cut(s) 289, 299, 546, 806, 912
TfiI GAWTC 2 cut(s) 613, 638
Tru1I TTAA 5 cut(s) 389, 549, 839, 972, 1020
Tru9I TTAA 5 cut(s) 389, 549, 839, 972, 1020
TscAI CASTG 1 cut(s) 573
TseFI GTSAC 2 cut(s) 376, 921
TseI GCWGC 2 cut(s) 829, 1118
Tsp45I GTSAC 2 cut(s) 376, 921
TspDTI ATGAA 5 cut(s) 141, 692, 722, 887, 992
TspRI CASTG 1 cut(s) 573
VneI GTGCAC 1 cut(s) 507
XapI RAATTY 1 cut(s) 806
XbaI TCTAGA 1 cut(s) 986
XceI RCATGY 2 cut(s) 442, 1111
XcmI CCANNNNNNNNNTGG 2 cut(s) 405, 656
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 5 cut(s) 174, 423, 491, 893, 987
Zsp2I ATGCAT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.