RLG00000002955

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001009
Physical Location & Seq
Reverse (-)
42243110 .. 42249386
6277 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002955

Sequence Viewer

Length: 969 bp
ATGTCAGGTAGCACAACATACAATGGCCATGGCTTAAACGAGTTTGTGCCGCAGAGGACGTCGGCTTATGTCAGTCAACAGGACTGGTTTGCAGTGGAGATGACTGTAAGGGAGACTCTTGACTTTGCAGGACGGTGTCAAGGTGTTGGATTCAAATATGATATACTAGTAGAACTAGCAAGAAGAGAAAAGATTGCGGGGATAAAACCCGATGGTGATCTTGATATATTCATGAAGTCATTAGCTCTTGGAGAGAAAGAGACAAGCCTTGTGGTAGAGTACATCATGAAGTGTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAGGCAATTAGCTTCTAATGGAGTTGGGGTGGTACTAACAGCAAGAGATGTGAATAGAGGCACAGAAGCTGTTGAAAAGCTTAAGGCCTCTGGTTTCTCTGATGTGGTCTTTCATCAGCTAGATGTAAATAACCCAACTACCATTGCTTCTCTGGCAGATTTTCTCAAAACCCAGTTTGGAAAGCTTGACATTTTGGTTAACAATGCAGGAGTCCTTCGATCCACATACCTCACCGATAACGTCGTAGTGGATCGTCAAAATGTTATTGCAAATGAGAAAGCCAAGAAAGAGCTAGGAGATGTCAATAACCTCACCGAGGAAAAAGTGGACAAGCTGGTTGAGGAATTTCTGGAGGATGTGAAGCTGGATTTGATAAAATCCAAAGGCTGGCCTCTAAACATATCTTCTTACATTGTATCAAAAGCAGCTCTGAATGCTTATAGAAGAGTCTTGGCAAAGAAGTATCCTGAAATTGCTACAAACGCAGTTAGTCCTGGCTTTACCAAAACAGATATCAACCAAAATACTGGGATTAACACAGTTGAAGAAGGTGCAGAAGGTCCTGTGAAGCTGGCTTTGATAGCTGACACTGGAATTTCTGGCCTCTACTTTGAAATGACTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

323

Amino Acids

35.26

Weight (kDa)

5.04

Isoelectric Point (pI)

23.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 98 - 185 7.8e-18 short chain dehydrogenase
KR PF08659 98 - 197 1.8e-07 KR domain
adh_short_C2 PF13561 99 - 285 5.2e-23 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 952
AatII GACGTC 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 714
AciI CCGC 2 cut(s) 50, 197
AclWI GGATC 2 cut(s) 540, 585
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 2 cut(s) 671, 921
AcuI CTGAAG 1 cut(s) 969
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 281, 360
AfiI CCNNNNNNNGG 3 cut(s) 306, 643, 714
AflII CTTAAG 1 cut(s) 407
AgsI TTSAA 4 cut(s) 154, 401, 872, 941
AhlI ACTAGT 1 cut(s) 166
AjnI CCWGG 1 cut(s) 820
Alw26I GTCTC 2 cut(s) 107, 254
AlwI GGATC 2 cut(s) 540, 585
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 4 cut(s) 25, 411, 716, 928
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 2 cut(s) 671, 921
AspS9I GGNCC 1 cut(s) 887
AsuHPI GGTGA 3 cut(s) 227, 550, 631
AvaII GGWCC 1 cut(s) 887
BalI TGGCCA 1 cut(s) 27
BbvI GCAGC 1 cut(s) 764
BccI CCATC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 822
BciVI GTATCC 1 cut(s) 801
BcoDI GTCTC 2 cut(s) 107, 254
BcuI ACTAGT 1 cut(s) 166
BfaI CTAG 4 cut(s) 167, 176, 446, 620
BfrI CTTAAG 1 cut(s) 407
BfuI GTATCC 1 cut(s) 801
BisI GCNGC 2 cut(s) 50, 753
BlsI GCNGC 2 cut(s) 51, 754
Bme1390I CCNGG 1 cut(s) 822
Bme18I GGWCC 1 cut(s) 887
BmgT120I GGNCC 1 cut(s) 887
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 822
BmrI ACTGGG 2 cut(s) 493, 864
BmuI ACTGGG 2 cut(s) 493, 864
BpmI CTGGAG 1 cut(s) 698
BpuEI CTTGAG 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 28, 642
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 643, 714
Bse1I ACTGG 4 cut(s) 89, 499, 859, 922
Bse3DI GCAATG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 216
BseBI CCWGG 1 cut(s) 822
BseDI CCNNGG 2 cut(s) 28, 642
BseGI GGATG 1 cut(s) 688
BseJI GATNNNNATC 1 cut(s) 216
BseLI CCNNNNNNNGG 3 cut(s) 306, 643, 714
BseMI GCAATG 1 cut(s) 468
BseNI ACTGG 4 cut(s) 89, 499, 859, 922
BseXI GCAGC 1 cut(s) 764
BsgI GTGCAG 1 cut(s) 900
BshFI GGCC 4 cut(s) 27, 413, 718, 930
BslI CCNNNNNNNGG 3 cut(s) 306, 643, 714
BsmAI GTCTC 2 cut(s) 107, 254
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 4 cut(s) 27, 413, 718, 930
Bsp143I GATC 3 cut(s) 217, 545, 577
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 2 cut(s) 50, 197
BspANI GGCC 4 cut(s) 27, 413, 718, 930
BspHI TCATGA 2 cut(s) 231, 285
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 2 cut(s) 540, 585
BspTI CTTAAG 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 4 cut(s) 89, 499, 859, 922
BssECI CCNNGG 2 cut(s) 28, 642
BssMI GATC 3 cut(s) 217, 545, 577
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 28
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 3 cut(s) 106, 135, 868
Bst6I CTCTTC 3 cut(s) 178, 766, 945
BstACI GRCGYC 1 cut(s) 59
BstAFI CTTAAG 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 716, 900
BstDSI CCRYGG 1 cut(s) 28
BstENI CCTNNNNNAGG 1 cut(s) 641
BstF5I GGATG 1 cut(s) 688
BstKTI GATC 3 cut(s) 220, 548, 580
BstMAI GTCTC 2 cut(s) 107, 254
BstMBI GATC 3 cut(s) 217, 545, 577
BstMWI GCNNNNNNNGC 4 cut(s) 479, 761, 809, 908
BstNI CCWGG 1 cut(s) 822
BstSCI CCNGG 1 cut(s) 820
BstV1I GCAGC 1 cut(s) 764
BstXI CCANNNNNNTGG 1 cut(s) 854
BsuI GTATCC 1 cut(s) 801
BsuRI GGCC 4 cut(s) 27, 413, 718, 930
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 1 cut(s) 688
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 2 cut(s) 99, 915
Cac8I GCNNGC 2 cut(s) 716, 900
CaiI CAGNNNCTG 1 cut(s) 395
CciI TCATGA 2 cut(s) 231, 285
Cfr13I GGNCC 1 cut(s) 887
Csp6I GTAC 2 cut(s) 280, 359
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 3 cut(s) 29, 232, 286
CviQI GTAC 2 cut(s) 280, 359
DpnI GATC 3 cut(s) 219, 547, 579
DpnII GATC 3 cut(s) 217, 545, 577
DrdI GACNNNNNNGTC 1 cut(s) 952
DseDI GACNNNNNNGTC 1 cut(s) 952
EaeI YGGCCR 1 cut(s) 25
Eam1104I CTCTTC 3 cut(s) 178, 766, 945
EarI CTCTTC 3 cut(s) 178, 766, 945
Eco130I CCWWGG 1 cut(s) 28
Eco147I AGGCCT 1 cut(s) 413
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 969
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 887
EcoRII CCWGG 1 cut(s) 820
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 3 cut(s) 32, 235, 289
FatI CATG 3 cut(s) 28, 231, 285
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 50, 753
FokI GGATG 1 cut(s) 695
Fsp4HI GCNGC 2 cut(s) 50, 753
FspBI CTAG 4 cut(s) 167, 176, 446, 620
GluI GCNGC 2 cut(s) 50, 753
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 4 cut(s) 27, 413, 718, 930
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 3 cut(s) 32, 235, 289
HincII GTYRAC 3 cut(s) 77, 526, 957
HindII GTYRAC 3 cut(s) 77, 526, 957
HindIII AAGCTT 2 cut(s) 404, 509
HinfI GANTC 5 cut(s) 115, 150, 537, 774, 953
HpaI GTTAAC 1 cut(s) 526
HphI GGTGA 3 cut(s) 227, 550, 631
Hpy166II GTNNAC 4 cut(s) 77, 526, 655, 957
Hpy188I TCNGA 2 cut(s) 427, 759
Hpy188III TCNNGA 6 cut(s) 119, 221, 232, 286, 677, 794
Hpy8I GTNNAC 4 cut(s) 77, 526, 655, 957
Hpy99I CGWCG 2 cut(s) 64, 572
HpyAV CCTTC 3 cut(s) 551, 869, 878
HpyCH4III ACNGT 3 cut(s) 106, 135, 868
HpyCH4IV ACGT 2 cut(s) 59, 567
HpyCH4V TGCA 5 cut(s) 92, 128, 533, 596, 881
HpyF10VI GCNNNNNNNGC 4 cut(s) 479, 761, 809, 908
HpySE526I ACGT 2 cut(s) 59, 567
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 3 cut(s) 32, 235, 289
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 3 cut(s) 217, 545, 577
LmnI GCTCC 1 cut(s) 296
Lsp1109I GCAGC 1 cut(s) 764
MaeI CTAG 4 cut(s) 167, 176, 446, 620
MaeII ACGT 2 cut(s) 59, 567
MalI GATC 3 cut(s) 219, 547, 579
MboI GATC 3 cut(s) 217, 545, 577
MboII GAAGA 5 cut(s) 195, 723, 783, 884, 962
MlsI TGGCCA 1 cut(s) 27
MluCI AATT 4 cut(s) 332, 671, 798, 921
MluNI TGGCCA 1 cut(s) 27
MlyI GAGTC 4 cut(s) 109, 546, 783, 962
MmeI TCCRAC 1 cut(s) 127
Mox20I TGGCCA 1 cut(s) 27
MscI TGGCCA 1 cut(s) 27
MseI TTAA 4 cut(s) 35, 408, 525, 861
Msp20I TGGCCA 1 cut(s) 27
MspCI CTTAAG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 822
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 1 cut(s) 822
MwoI GCNNNNNNNGC 4 cut(s) 479, 761, 809, 908
NcoI CCATGG 1 cut(s) 28
NdeII GATC 3 cut(s) 217, 545, 577
NlaIII CATG 3 cut(s) 32, 235, 289
NlaIV GGNNCC 1 cut(s) 298
PagI TCATGA 2 cut(s) 231, 285
PceI AGGCCT 1 cut(s) 413
PctI GAATGC 1 cut(s) 766
PfeI GAWTC 1 cut(s) 150
PflFI GACNNNGTC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 714
PkrI GCNGC 2 cut(s) 51, 754
PleI GAGTC 4 cut(s) 109, 545, 782, 961
PpsI GAGTC 4 cut(s) 109, 545, 782, 961
PpuMI RGGWCCY 1 cut(s) 887
Psp5II RGGWCCY 1 cut(s) 887
Psp6I CCWGG 1 cut(s) 820
PspGI CCWGG 1 cut(s) 820
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 887
PspPPI RGGWCCY 1 cut(s) 887
PstNI CAGNNNCTG 1 cut(s) 395
PsyI GACNNNGTC 1 cut(s) 135
RsaI GTAC 2 cut(s) 281, 360
RsaNI GTAC 2 cut(s) 280, 359
SaqAI TTAA 4 cut(s) 35, 408, 525, 861
SatI GCNGC 2 cut(s) 50, 753
Sau3AI GATC 3 cut(s) 217, 545, 577
Sau96I GGNCC 1 cut(s) 887
SchI GAGTC 4 cut(s) 109, 546, 783, 962
ScrFI CCNGG 1 cut(s) 822
SinI GGWCC 1 cut(s) 887
SmlI CTYRAG 2 cut(s) 317, 407
SmoI CTYRAG 2 cut(s) 317, 407
SpeI ACTAGT 1 cut(s) 166
Sse9I AATT 4 cut(s) 332, 671, 798, 921
SseBI AGGCCT 1 cut(s) 413
SsiI CCGC 2 cut(s) 50, 197
SspMI CTAG 4 cut(s) 167, 176, 446, 620
StuI AGGCCT 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 820
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 3 cut(s) 106, 135, 868
TaiI ACGT 2 cut(s) 62, 570
TaqI TCGA 1 cut(s) 544
TasI AATT 4 cut(s) 332, 671, 798, 921
TatI WGTACW 1 cut(s) 279
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 4 cut(s) 35, 408, 525, 861
Tru9I TTAA 4 cut(s) 35, 408, 525, 861
TscAI CASTG 2 cut(s) 99, 922
TseI GCWGC 1 cut(s) 752
TspDTI ATGAA 4 cut(s) 220, 248, 302, 428
TspRI CASTG 2 cut(s) 99, 922
Tth111I GACNNNGTC 1 cut(s) 135
Van91I CCANNNNNTGG 1 cut(s) 714
Vha464I CTTAAG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 887
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 2 cut(s) 671, 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XspI CTAG 4 cut(s) 167, 176, 446, 620
ZraI GACGTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.