Rh7BG449300

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001009
Physical Location & Seq
Forward (+)
52510161 .. 52512168
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG449300.1

Sequence Viewer

Length: 690 bp
ATGCCTGGTCCGACGGCGCGTCCTTGGGACGTCGTCGTCGGACAGGCCAAAGAGGACTTACTGTCCGGTGGTGGTTCCTCGTGGACCAGTGGTGGTCGAAACGGAAGTCGTACGTTGAAGGCCGCAAGGTTTCCAATCCGGGCCTTGTCATTGCCGCTTGGAAGCAATGGTAAGAGGGATAAGCAGAGGTGGAGGTGTGGTGGAATGCAGGCGGGGCTGCAAGATCTCTTCCATCTTTGGGCATCGACGATGAAGAAAAGAGATTTTGGGTTGGCTGGGTGGACGGCTTGGACTGCAGGGGTGCGATCTGATTTGACTGCAGATCGATACCGCGTTGGCCAGTGGAAGGGCGGCGGCCAGTGGTTGTCGTCGATTTCGGGCCACCTCAGATCTACTTTTGGGTGGTCGAGTGATGTCGGGATTTGGCAGCCAGGGAGTGGGAGTGACGGGAATTCTGGTGGAGGTATATACATACAACCTATTGATTTTACTCTTTCCGAGAAAGTTATTAGTGCTTCAGAATCAGAAGATGAACTATATCCAAAAACAAGATCAGAAGCTGATCAAGGTCATCTCAGGGATATATTTTGCTCGGGGAGTGCTGGTGGATATACTCTATTTTGGGTTGGTTTTGGGATGTGTGCAGGGTCTTCGTTGGAAAATAGTCACTTTTATCACGTGTTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

24.74

Weight (kDa)

9.05

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
AatII GACGTC 1 cut(s) 33
AccB7I CCANNNNNTGG 1 cut(s) 437
AccII CGCG 2 cut(s) 19, 333
AciI CCGC 6 cut(s) 123, 155, 212, 331, 351, 354
AcoI YGGCCR 2 cut(s) 337, 355
AcsI RAATTY 1 cut(s) 451
AcuI CTGAAG 1 cut(s) 501
AcvI CACGTG 1 cut(s) 679
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 3 cut(s) 238, 346, 437
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 1 cut(s) 118
AhdI GACNNNNNGTC 2 cut(s) 18, 61
AjnI CCWGG 2 cut(s) 4, 430
AluBI AGCT 1 cut(s) 560
AluI AGCT 1 cut(s) 560
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 592
AoxI GGCC 6 cut(s) 45, 120, 141, 337, 355, 379
ApeKI GCWGC 2 cut(s) 217, 427
ApoI RAATTY 1 cut(s) 451
ArsI GACNNNNNNTTYG 2 cut(s) 91, 123
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 4 cut(s) 8, 84, 141, 379
AsuC2I CCSGG 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 592
AvaII GGWCC 2 cut(s) 8, 84
BalI TGGCCA 1 cut(s) 339
BauI CACGAG 1 cut(s) 79
BbrPI CACGTG 1 cut(s) 679
BbsI GAAGAC 1 cut(s) 642
BbvI GCAGC 2 cut(s) 204, 439
BccI CCATC 1 cut(s) 240
BceAI ACGGC 2 cut(s) 30, 300
BcgI CGANNNNNNTGC 2 cut(s) 633, 667
BciT130I CCWGG 2 cut(s) 6, 432
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 1 cut(s) 140
BfmI CTRYAG 2 cut(s) 294, 318
BglII AGATCT 2 cut(s) 223, 389
BisI GCNGC 6 cut(s) 123, 155, 218, 352, 355, 428
BlsI GCNGC 6 cut(s) 124, 156, 219, 353, 356, 429
Bme1390I CCNGG 3 cut(s) 6, 140, 432
Bme18I GGWCC 2 cut(s) 8, 84
BmeRI GACNNNNNGTC 2 cut(s) 18, 61
BmeT110I CYCGRG 1 cut(s) 592
BmgT120I GGNCC 4 cut(s) 8, 84, 141, 379
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 3 cut(s) 6, 140, 432
BmsI GCATC 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 140
Bsa29I ATCGAT 1 cut(s) 325
BsaAI YACGTR 1 cut(s) 679
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 23, 431
BsaWI WCCGGW 1 cut(s) 65
BsaXI ACNNNNNCTCC 6 cut(s) 184, 214, 427, 457, 589, 619
Bsc4I CCNNNNNNNGG 3 cut(s) 238, 346, 437
Bse1I ACTGG 3 cut(s) 87, 340, 358
Bse3DI GCAATG 2 cut(s) 149, 172
BseBI CCWGG 2 cut(s) 6, 432
BseCI ATCGAT 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 23, 431
BseGI GGATG 1 cut(s) 642
BseLI CCNNNNNNNGG 3 cut(s) 238, 346, 437
BseMI GCAATG 2 cut(s) 149, 172
BseMII CTCAG 2 cut(s) 400, 589
BseNI ACTGG 3 cut(s) 87, 340, 358
BseXI GCAGC 2 cut(s) 204, 439
BseYI CCCAGC 1 cut(s) 275
BsgI GTGCAG 1 cut(s) 663
Bsh1236I CGCG 2 cut(s) 19, 333
BshFI GGCC 6 cut(s) 47, 122, 143, 339, 357, 381
BshVI ATCGAT 1 cut(s) 325
BsiHKCI CYCGRG 1 cut(s) 592
BsiSI CCGG 2 cut(s) 66, 139
BsiWI CGTACG 1 cut(s) 110
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 3 cut(s) 238, 346, 437
BsmFI GGGAC 1 cut(s) 41
BsmI GAATGC 1 cut(s) 210
BsnI GGCC 6 cut(s) 47, 122, 143, 339, 357, 381
BsoBI CYCGRG 1 cut(s) 592
Bsp143I GATC 6 cut(s) 223, 305, 322, 389, 551, 562
BspACI CCGC 6 cut(s) 123, 155, 212, 331, 351, 354
BspANI GGCC 6 cut(s) 47, 122, 143, 339, 357, 381
BspCNI CTCAG 2 cut(s) 399, 588
BspDI ATCGAT 1 cut(s) 325
BspFNI CGCG 2 cut(s) 19, 333
BspLI GGNNCC 1 cut(s) 76
BspMAI CTGCAG 2 cut(s) 298, 322
BsrDI GCAATG 2 cut(s) 149, 172
BsrI ACTGG 3 cut(s) 87, 340, 358
BssECI CCNNGG 2 cut(s) 23, 431
BssMI GATC 6 cut(s) 223, 305, 322, 389, 551, 562
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 79
BssT1I CCWWGG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 79
Bst2UI CCWGG 2 cut(s) 6, 432
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 233
BstACI GRCGYC 1 cut(s) 30
BstBAI YACGTR 1 cut(s) 679
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 386, 575
BstF5I GGATG 1 cut(s) 642
BstFNI CGCG 2 cut(s) 19, 333
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 6 cut(s) 226, 308, 325, 392, 554, 565
BstMBI GATC 6 cut(s) 223, 305, 322, 389, 551, 562
BstMWI GCNNNNNNNGC 2 cut(s) 214, 293
BstNI CCWGG 2 cut(s) 6, 432
BstSCI CCNGG 3 cut(s) 4, 138, 430
BstSFI CTRYAG 2 cut(s) 294, 318
BstUI CGCG 2 cut(s) 19, 333
BstV1I GCAGC 2 cut(s) 204, 439
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 2 cut(s) 223, 389
BstYI RGATCY 2 cut(s) 223, 389
Bsu15I ATCGAT 1 cut(s) 325
BsuRI GGCC 6 cut(s) 47, 122, 143, 339, 357, 381
BsuTUI ATCGAT 1 cut(s) 325
BtsCI GGATG 1 cut(s) 642
BtsIMutI CAGTG 3 cut(s) 94, 347, 365
Cac8I GCNNGC 1 cut(s) 210
CaiI CAGNNNCTG 1 cut(s) 560
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 4 cut(s) 8, 84, 141, 379
ClaI ATCGAT 1 cut(s) 325
CseI GACGC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 111
CviQI GTAC 1 cut(s) 111
DdeI CTNAG 2 cut(s) 386, 575
DpnI GATC 6 cut(s) 225, 307, 324, 391, 553, 564
DpnII GATC 6 cut(s) 223, 305, 322, 389, 551, 562
DrdI GACNNNNNNGTC 1 cut(s) 35
DriI GACNNNNNGTC 2 cut(s) 18, 61
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 2 cut(s) 337, 355
Eam1104I CTCTTC 1 cut(s) 233
Eam1105I GACNNNNNGTC 2 cut(s) 18, 61
EarI CTCTTC 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 23
Eco47I GGWCC 2 cut(s) 8, 84
Eco57I CTGAAG 1 cut(s) 501
Eco72I CACGTG 1 cut(s) 679
Eco88I CYCGRG 1 cut(s) 592
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 2 cut(s) 4, 430
EcoT14I CCWWGG 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 23
FaiI YATR 7 cut(s) 467, 469, 473, 538, 584, 612, 688
FaqI GGGAC 1 cut(s) 41
FauI CCCGC 1 cut(s) 205
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 6 cut(s) 123, 155, 218, 352, 355, 428
FokI GGATG 1 cut(s) 649
Fsp4HI GCNGC 6 cut(s) 123, 155, 218, 352, 355, 428
GlaI GCGC 1 cut(s) 18
GluI GCNGC 6 cut(s) 123, 155, 218, 352, 355, 428
GsaI CCCAGC 1 cut(s) 279
HaeIII GGCC 6 cut(s) 47, 122, 143, 339, 357, 381
HapII CCGG 2 cut(s) 66, 139
HgaI GACGC 1 cut(s) 8
HhaI GCGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 1 cut(s) 521
HpaII CCGG 2 cut(s) 66, 139
Hpy166II GTNNAC 2 cut(s) 84, 282
Hpy188I TCNGA 8 cut(s) 12, 41, 310, 389, 499, 520, 526, 556
Hpy188III TCNNGA 1 cut(s) 418
Hpy8I GTNNAC 2 cut(s) 84, 282
Hpy99I CGWCG 6 cut(s) 16, 35, 38, 41, 250, 373
HpyAV CCTTC 2 cut(s) 112, 340
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 3 cut(s) 30, 113, 678
HpyCH4V TGCA 5 cut(s) 208, 220, 296, 320, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 293
HpyF3I CTNAG 2 cut(s) 386, 575
HpySE526I ACGT 3 cut(s) 30, 113, 678
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 1 cut(s) 17
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 6 cut(s) 223, 305, 322, 389, 551, 562
Lsp1109I GCAGC 2 cut(s) 204, 439
LweI GCATC 1 cut(s) 251
MaeII ACGT 3 cut(s) 30, 113, 678
MaeIII GTNAC 2 cut(s) 443, 665
MalI GATC 6 cut(s) 225, 307, 324, 391, 553, 564
MboI GATC 6 cut(s) 223, 305, 322, 389, 551, 562
MboII GAAGA 4 cut(s) 220, 265, 539, 642
MflI RGATCY 2 cut(s) 223, 389
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 1 cut(s) 451
MluNI TGGCCA 1 cut(s) 339
MmeI TCCRAC 3 cut(s) 19, 35, 636
MnlI CCTC 7 cut(s) 46, 88, 168, 180, 186, 395, 455
Mox20I TGGCCA 1 cut(s) 339
MscI TGGCCA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 339
MspI CCGG 2 cut(s) 66, 139
MspR9I CCNGG 3 cut(s) 6, 140, 432
Mva1269I GAATGC 1 cut(s) 210
MvaI CCWGG 2 cut(s) 6, 432
MvnI CGCG 2 cut(s) 19, 333
MwoI GCNNNNNNNGC 2 cut(s) 214, 293
NciI CCSGG 1 cut(s) 140
NdeII GATC 6 cut(s) 223, 305, 322, 389, 551, 562
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 443, 665
PctI GAATGC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 521
Pfl23II CGTACG 1 cut(s) 110
PflFI GACNNNGTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 6 cut(s) 124, 156, 219, 353, 356, 429
PmaCI CACGTG 1 cut(s) 679
PmlI CACGTG 1 cut(s) 679
Ppu21I YACGTR 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 4, 430
PspCI CACGTG 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 2 cut(s) 4, 430
PspLI CGTACG 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 4 cut(s) 8, 84, 141, 379
PstI CTGCAG 2 cut(s) 298, 322
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 2 cut(s) 223, 389
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
SatI GCNGC 6 cut(s) 123, 155, 218, 352, 355, 428
Sau3AI GATC 6 cut(s) 223, 305, 322, 389, 551, 562
Sau96I GGNCC 4 cut(s) 8, 84, 141, 379
ScrFI CCNGG 3 cut(s) 6, 140, 432
SfaNI GCATC 1 cut(s) 251
SfcI CTRYAG 2 cut(s) 294, 318
SinI GGWCC 2 cut(s) 8, 84
Sse9I AATT 1 cut(s) 451
SsiI CCGC 6 cut(s) 123, 155, 212, 331, 351, 354
StyD4I CCNGG 3 cut(s) 4, 138, 430
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 3 cut(s) 33, 116, 681
TaqI TCGA 5 cut(s) 97, 245, 325, 371, 407
TasI AATT 1 cut(s) 451
TauI GCSGC 4 cut(s) 125, 157, 354, 357
TfiI GAWTC 1 cut(s) 521
TscAI CASTG 3 cut(s) 94, 347, 365
TseFI GTSAC 2 cut(s) 443, 665
TseI GCWGC 2 cut(s) 217, 427
Tsp45I GTSAC 2 cut(s) 443, 665
TspDTI ATGAA 2 cut(s) 266, 546
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 3 cut(s) 94, 347, 365
Tth111I GACNNNGTC 1 cut(s) 32
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 8, 84
XapI RAATTY 1 cut(s) 451
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.