Rroxscaffold_3G00225320

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001009
Physical Location & Seq
Forward (+)
8586950 .. 8590256
3307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225320.1

Sequence Viewer

Length: 918 bp
ATGTATGACGTACCAACCGTTATAAGTGAAAGGAAGATTATGATTCCTATCTCAGATGTCAAGCTAGAACCCAGAAGGTGTGAGTGGCCAAAGGTCATGGCTGATCAGCTTTCCAGCATGGGTATCCCAGAAGCTCAGCAGCCGCATATATTGGAAAAAACATTTGAGGTGGCTCAAGTGACCAACCCCGAGTTGCGAATTAATGTGATGATGGTGTACCTGACTGTGTACAGGTTTCCAGCCATCTCAAGTGACATTATGGTGAATGAGATAGTCTTGGCCTTCCTGGTTTCACTAGAAATGCCATCTTTTAGTATGAGAGTCGAGAGCGAAAACGTGCCCTCTCAAAACCCTAGCGCCGCCTCCTGTAGGCTGCTTCTAGGGAGGTCTTCGACATCTCCAGTTCAAGGTTCCTCTCAACGATTATGCATTGCGGTGGATCTGTTCCATTTTTGTTGCAAGGAATGGCGGGTTGCGGTTTGTGTAGCAGCGATCCTCTTCATTCTCCGGCCAAGACGCAAACGACTTCGTGATCATAGTCTGAGGAGGAGATCTGGAATCTGGTATGGATCGGGGCATCATTCCTTTAGGGTTTGGCAGGTCTTTGATGGTGGGACAAGGCTGCTGGTGGCTGCGCTGCAGAGCGGTGGCAGAGTCCTGCTTTGGTGGATAGCTCTGAGGCGTTTGATGCGGAAGCTATTGGTGTGGTCAACGATGGTGGTCTGGATGCATGTGGCGCCAGAGTTGCTTGGATTTTGGGTTGATTTACAGGTAGTGCTGGCTTGGCTATTCGGAGTAGCTTTTCTGGCGGCTTGGAATCGCTGGTTTGGCTGTTTGGAGATAGTTGGTATGGTGGCTGTATTGGGTACAGATGACGACTGCATTCGACGACTGCCGACGGCTACAACGGGACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

305

Amino Acids

34.76

Weight (kDa)

9.4

Isoelectric Point (pI)

56.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 23
Acc36I ACCTGC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 736
AccBSI CCGCTC 1 cut(s) 645
AciI CCGC 8 cut(s) 143, 360, 434, 469, 476, 645, 691, 809
AclWI GGATC 3 cut(s) 447, 487, 577
AcoI YGGCCR 2 cut(s) 86, 509
AcyI GRCGYC 1 cut(s) 737
AfaI GTAC 4 cut(s) 12, 218, 230, 868
AfiI CCNNNNNNNGG 2 cut(s) 369, 407
AgsI TTSAA 1 cut(s) 407
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 6 cut(s) 64, 109, 134, 674, 697, 800
AluI AGCT 6 cut(s) 64, 109, 134, 674, 697, 800
AlwI GGATC 3 cut(s) 447, 487, 577
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 3 cut(s) 86, 279, 509
ApeKI GCWGC 6 cut(s) 139, 373, 488, 622, 632, 637
AseI ATTAAT 1 cut(s) 201
AspLEI GCGC 3 cut(s) 359, 637, 739
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 342
BalI TGGCCA 1 cut(s) 88
BanI GGYRCC 1 cut(s) 736
BbsI GAAGAC 1 cut(s) 381
BbvI GCAGC 6 cut(s) 151, 360, 500, 609, 619, 624
BccI CCATC 5 cut(s) 205, 251, 313, 602, 709
BceAI ACGGC 1 cut(s) 915
BciT130I CCWGG 1 cut(s) 287
BciVI GTATCC 1 cut(s) 134
BclI TGATCA 2 cut(s) 103, 532
BfaI CTAG 5 cut(s) 65, 296, 354, 380, 916
BfmI CTRYAG 2 cut(s) 367, 638
BfoI RGCGCY 2 cut(s) 360, 740
BfuAI ACCTGC 1 cut(s) 589
BfuI GTATCC 1 cut(s) 134
BglII AGATCT 1 cut(s) 551
BisI GCNGC 9 cut(s) 140, 143, 360, 374, 489, 623, 633, 638, 810
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 9 cut(s) 141, 144, 361, 375, 490, 624, 634, 639, 811
Bme1390I CCNGG 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 188
BmiI GGNNCC 2 cut(s) 412, 738
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 586, 678, 717
BpiI GAAGAC 1 cut(s) 381
BpmI CTGGAG 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 159, 232
BsaBI GATNNNNATC 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 737
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 407
Bse1I ACTGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 47
BseBI CCWGG 1 cut(s) 287
BseGI GGATG 1 cut(s) 732
BseJI GATNNNNATC 1 cut(s) 47
BseLI CCNNNNNNNGG 2 cut(s) 369, 407
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 4 cut(s) 66, 149, 533, 668
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 2 cut(s) 559, 562
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 6 cut(s) 151, 360, 500, 609, 619, 624
BshFI GGCC 3 cut(s) 88, 281, 511
BshNI GGYRCC 1 cut(s) 736
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 508
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 2 cut(s) 369, 407
BsmFI GGGAC 1 cut(s) 628
BsmI GAATGC 1 cut(s) 882
BsnI GGCC 3 cut(s) 88, 281, 511
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 342
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 6 cut(s) 103, 439, 492, 532, 551, 569
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 8 cut(s) 143, 360, 434, 469, 476, 645, 691, 809
BspANI GGCC 3 cut(s) 88, 281, 511
BspCNI CTCAG 4 cut(s) 65, 148, 534, 669
BspLI GGNNCC 2 cut(s) 412, 738
BspMAI CTGCAG 1 cut(s) 642
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 3 cut(s) 447, 487, 577
BspT107I GGYRCC 1 cut(s) 736
BsrBI CCGCTC 1 cut(s) 645
BsrDI GCAATG 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 1 cut(s) 401
BssMI GATC 6 cut(s) 103, 439, 492, 532, 551, 569
BssNI GRCGYC 1 cut(s) 737
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 19, 226
Bst6I CTCTTC 1 cut(s) 503
BstACI GRCGYC 1 cut(s) 737
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 4 cut(s) 52, 135, 542, 677
BstENI CCTNNNNNAGG 1 cut(s) 367
BstF5I GGATG 1 cut(s) 732
BstH2I RGCGCY 2 cut(s) 360, 740
BstHHI GCGC 3 cut(s) 359, 637, 739
BstKTI GATC 6 cut(s) 106, 442, 495, 535, 554, 572
BstMBI GATC 6 cut(s) 103, 439, 492, 532, 551, 569
BstMWI GCNNNNNNNGC 6 cut(s) 688, 736, 745, 784, 806, 828
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 2 cut(s) 367, 638
BstSLI GKGCMC 1 cut(s) 342
BstV1I GCAGC 6 cut(s) 151, 360, 500, 609, 619, 624
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 2 cut(s) 439, 551
BstYI RGATCY 2 cut(s) 439, 551
BsuI GTATCC 1 cut(s) 134
BsuRI GGCC 3 cut(s) 88, 281, 511
BtsCI GGATG 1 cut(s) 732
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 1 cut(s) 780
CfoI GCGC 3 cut(s) 359, 637, 739
CseI GACGC 1 cut(s) 525
Csp6I GTAC 4 cut(s) 11, 217, 229, 867
CspCI CAANNNNNGTGG 2 cut(s) 699, 734
CviAII CATG 3 cut(s) 97, 118, 731
CviQI GTAC 4 cut(s) 11, 217, 229, 867
DdeI CTNAG 4 cut(s) 52, 135, 542, 677
DinI GGCGCC 1 cut(s) 738
DpnI GATC 6 cut(s) 105, 441, 494, 534, 553, 571
DpnII GATC 6 cut(s) 103, 439, 492, 532, 551, 569
EaeI YGGCCR 2 cut(s) 86, 509
Eam1104I CTCTTC 1 cut(s) 503
EarI CTCTTC 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 285
EcoT22I ATGCAT 2 cut(s) 431, 732
EgeI GGCGCC 1 cut(s) 738
EheI GGCGCC 1 cut(s) 738
FaeI CATG 3 cut(s) 100, 121, 734
FaqI GGGAC 1 cut(s) 628
FatI CATG 3 cut(s) 96, 117, 730
FauI CCCGC 1 cut(s) 462
FbaI TGATCA 2 cut(s) 103, 532
Fnu4HI GCNGC 9 cut(s) 140, 143, 360, 374, 489, 623, 633, 638, 810
FokI GGATG 1 cut(s) 739
Fsp4HI GCNGC 9 cut(s) 140, 143, 360, 374, 489, 623, 633, 638, 810
FspBI CTAG 5 cut(s) 65, 296, 354, 380, 916
GlaI GCGC 3 cut(s) 358, 636, 738
GluI GCNGC 9 cut(s) 140, 143, 360, 374, 489, 623, 633, 638, 810
GsuI CTGGAG 1 cut(s) 384
HaeII RGCGCY 2 cut(s) 360, 740
HaeIII GGCC 3 cut(s) 88, 281, 511
HapII CCGG 1 cut(s) 508
HgaI GACGC 1 cut(s) 525
HhaI GCGC 3 cut(s) 359, 637, 739
Hin1I GRCGYC 1 cut(s) 737
Hin1II CATG 3 cut(s) 100, 121, 734
Hin6I GCGC 3 cut(s) 357, 635, 737
HinP1I GCGC 3 cut(s) 357, 635, 737
HincII GTYRAC 1 cut(s) 711
HindII GTYRAC 1 cut(s) 711
HinfI GANTC 6 cut(s) 43, 321, 558, 654, 817, 912
HpaII CCGG 1 cut(s) 508
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 3 cut(s) 217, 229, 711
Hpy188I TCNGA 4 cut(s) 55, 543, 678, 794
Hpy188III TCNNGA 4 cut(s) 325, 530, 555, 724
Hpy8I GTNNAC 3 cut(s) 217, 229, 711
Hpy99I CGWCG 2 cut(s) 891, 901
HpyAV CCTTC 2 cut(s) 69, 292
HpyCH4III ACNGT 2 cut(s) 19, 226
HpyCH4IV ACGT 2 cut(s) 9, 336
HpyCH4V TGCA 5 cut(s) 429, 459, 640, 730, 882
HpyF10VI GCNNNNNNNGC 6 cut(s) 688, 736, 745, 784, 806, 828
HpyF3I CTNAG 4 cut(s) 52, 135, 542, 677
HpySE526I ACGT 2 cut(s) 9, 336
Hsp92I GRCGYC 1 cut(s) 737
Hsp92II CATG 3 cut(s) 100, 121, 734
HspAI GCGC 3 cut(s) 357, 635, 737
KasI GGCGCC 1 cut(s) 736
Ksp22I TGATCA 2 cut(s) 103, 532
Kzo9I GATC 6 cut(s) 103, 439, 492, 532, 551, 569
Lsp1109I GCAGC 6 cut(s) 151, 360, 500, 609, 619, 624
LweI GCATC 3 cut(s) 586, 678, 717
MaeI CTAG 5 cut(s) 65, 296, 354, 380, 916
MaeII ACGT 2 cut(s) 9, 336
MaeIII GTNAC 2 cut(s) 178, 251
MalI GATC 6 cut(s) 105, 441, 494, 534, 553, 571
MbiI CCGCTC 1 cut(s) 645
MboI GATC 6 cut(s) 103, 439, 492, 532, 551, 569
MboII GAAGA 3 cut(s) 46, 381, 490
MflI RGATCY 2 cut(s) 439, 551
MhlI GDGCHC 1 cut(s) 342
MlsI TGGCCA 1 cut(s) 88
MluCI AATT 1 cut(s) 198
MluNI TGGCCA 1 cut(s) 88
Mly113I GGCGCC 1 cut(s) 737
MlyI GAGTC 3 cut(s) 330, 663, 906
MnlI CCTC 9 cut(s) 160, 352, 373, 378, 424, 506, 537, 540, 672
Mox20I TGGCCA 1 cut(s) 88
Mph1103I ATGCAT 2 cut(s) 431, 732
MscI TGGCCA 1 cut(s) 88
MseI TTAA 1 cut(s) 201
MslI CAYNNNNRTG 2 cut(s) 260, 434
Msp20I TGGCCA 1 cut(s) 88
MspI CCGG 1 cut(s) 508
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 882
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 6 cut(s) 688, 736, 745, 784, 806, 828
NarI GGCGCC 1 cut(s) 737
NdeII GATC 6 cut(s) 103, 439, 492, 532, 551, 569
NlaIII CATG 3 cut(s) 100, 121, 734
NlaIV GGNNCC 2 cut(s) 412, 738
NmuCI GTSAC 2 cut(s) 178, 251
NsiI ATGCAT 2 cut(s) 431, 732
NspI RCATGY 1 cut(s) 734
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 882
PfeI GAWTC 3 cut(s) 43, 558, 817
PkrI GCNGC 9 cut(s) 141, 144, 361, 375, 490, 624, 634, 639, 811
PleI GAGTC 3 cut(s) 329, 662, 906
PluTI GGCGCC 1 cut(s) 740
PpsI GAGTC 3 cut(s) 329, 662, 906
PshBI ATTAAT 1 cut(s) 201
PsiI TTATAA 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 412, 738
PstI CTGCAG 1 cut(s) 642
PsuI RGATCY 2 cut(s) 439, 551
RsaI GTAC 4 cut(s) 12, 218, 230, 868
RsaNI GTAC 4 cut(s) 11, 217, 229, 867
RseI CAYNNNNRTG 2 cut(s) 260, 434
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 9 cut(s) 140, 143, 360, 374, 489, 623, 633, 638, 810
Sau3AI GATC 6 cut(s) 103, 439, 492, 532, 551, 569
SchI GAGTC 3 cut(s) 330, 663, 906
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 342
SfaNI GCATC 3 cut(s) 586, 678, 717
SfcI CTRYAG 2 cut(s) 367, 638
SfoI GGCGCC 1 cut(s) 738
SmiMI CAYNNNNRTG 2 cut(s) 260, 434
SmlI CTYRAG 2 cut(s) 174, 247
SmoI CTYRAG 2 cut(s) 174, 247
Sse9I AATT 1 cut(s) 198
SsiI CCGC 8 cut(s) 143, 360, 434, 469, 476, 645, 691, 809
SspDI GGCGCC 1 cut(s) 736
SspMI CTAG 5 cut(s) 65, 296, 354, 380, 916
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 19, 226
TaiI ACGT 2 cut(s) 12, 339
TaqI TCGA 3 cut(s) 324, 392, 886
TasI AATT 1 cut(s) 198
TatI WGTACW 1 cut(s) 228
TauI GCSGC 3 cut(s) 145, 362, 812
TfiI GAWTC 3 cut(s) 43, 558, 817
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseFI GTSAC 2 cut(s) 178, 251
TseI GCWGC 6 cut(s) 139, 373, 488, 622, 632, 637
Tsp45I GTSAC 2 cut(s) 178, 251
TspDTI ATGAA 1 cut(s) 490
VspI ATTAAT 1 cut(s) 201
XagI CCTNNNNNAGG 1 cut(s) 367
XceI RCATGY 1 cut(s) 734
XspI CTAG 5 cut(s) 65, 296, 354, 380, 916
Zsp2I ATGCAT 2 cut(s) 431, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.