FvH4_6g41380

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000434
Physical Location & Seq
Forward (+)
32537433 .. 32539550
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41380.t2

Sequence Viewer

Length: 1218 bp
ATGGGAGGACACAATCTAGTCTTGATTATCTTTTCGGTATTTTTGTTATCATCCTCAGCATATGCAAGTAGAGTCGCACAGTTTTTAGCTCCTGGTGACTTTGATGTGACAAGTCCGAAATATGGTGGGAAGCCTAATACTGATATTAGTCAGCCTTTGGCAAATGCTTGGAAAGATGCTTGTGCAGCAACAACGCCAAGTAGAGTTATTGTTCCAAAAGGGACATTTCAACTAAAAGGAGCAGTTTTCAAAGGTCCTTGTAAGGCTCCTATTACGGTTCAGGTTGATGGCATACTGCAGGCGCCACCAATTGAAGCCCAATTAGCAAATAAAGAATTTTGGGTCCAGTTTTTAGAGGTTGAGAGGCTCACTGTATCAGGTACTGGAACATTTGATGGTCAAGGACAAAATTCTTGGAAAGACAACGACTGCAATAAAAATCCAAACTGTGGAGGTCTAGCCATTAATGTGAGATTCGACAAAGTGAAAAATTCATTAGTAAGGGATGTGACATCACTTAACAGCAAAAATTTCCACTTCAATATTTTAGGGTGTGAACATCTTACATTCCAACATGTCACCATCAAAGCACCGGGAGATAGCCCTAACACAGATGGAATACATATGGGGCGCTCAACCGGGATTAACATTACGGACATGAACATCGGAACCGGAGACGATTGCATATCAGTTGGTGATGGTACCAGACAACTAACTGTAAGTAAGGTAAGTTGTGGACCAGGTCATGGAATAAGTATCGGAAGTCTCGGAAGGTACGACAATGAAGATGACGTCAGTGGACTCAATATTAGAGATTGTACCCTTAGTAATACACTGAATGGTGTTAGAATCAAGACATTTCCTGCTTCTCCTAAAGCTACCACCGCCTCGGATATTCACTTCGAGAAAATCACAATGAATAATGTTGCTAACCCAGTCCTCATTGACCAAGAATACTGCCCATGGGGTCAGTGTAATAAACAGATTCCATCAAAAGTCAAGATCAGCAATGTGAGCTTCAAGAACATTATAGGGACAACTTCAACTGCGGAGGCTTTGAAGATTGTATGCGCTAAAGGCTTGCATTGTGATCAAGTGGTACTGAGTGACATAGATCTCAAGTTAAGTGGCAAAGGAACTCTTACATCTCATTGTGCAAACGTGCAACCCACGATTGCTCGAGTGCCACCGCCTCTTGCTTGTGCTACCAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

406

Amino Acids

43.18

Weight (kDa)

8.33

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 1 - 182 8.3e-62 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 795
Acc65I GGTACC 1 cut(s) 701
AccB1I GGYRCC 2 cut(s) 301, 701
AccB7I CCANNNNNTGG 2 cut(s) 449, 746
AciI CCGC 3 cut(s) 885, 1049, 1190
AcsI RAATTY 4 cut(s) 335, 409, 490, 529
AcyI GRCGYC 2 cut(s) 302, 792
AfaI GTAC 5 cut(s) 382, 703, 776, 820, 1101
AfiI CCNNNNNNNGG 3 cut(s) 122, 449, 746
AflIII ACRYGT 1 cut(s) 574
AgsI TTSAA 7 cut(s) 230, 250, 314, 541, 1021, 1044, 1060
AjnI CCWGG 2 cut(s) 91, 739
AluBI AGCT 3 cut(s) 89, 878, 1017
AluI AGCT 3 cut(s) 89, 878, 1017
Alw26I GTCTC 2 cut(s) 669, 770
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 1179
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 4 cut(s) 335, 409, 490, 529
AseI ATTAAT 1 cut(s) 465
Asp718I GGTACC 1 cut(s) 701
AspLEI GCGC 3 cut(s) 304, 633, 1073
AspS9I GGNCC 3 cut(s) 254, 343, 737
AsuC2I CCSGG 2 cut(s) 594, 640
AsuHPI GGTGA 3 cut(s) 107, 571, 707
AvaI CYCGRG 1 cut(s) 1179
AvaII GGWCC 3 cut(s) 254, 343, 737
BanI GGYRCC 2 cut(s) 301, 701
BbvCI CCTCAGC 1 cut(s) 55
BbvI GCAGC 1 cut(s) 197
BccI CCATC 6 cut(s) 281, 389, 590, 608, 692, 997
BciT130I CCWGG 2 cut(s) 93, 741
BclI TGATCA 1 cut(s) 1090
BcnI CCSGG 2 cut(s) 594, 640
BcoDI GTCTC 2 cut(s) 669, 770
BfaI CTAG 2 cut(s) 17, 458
BfmI CTRYAG 1 cut(s) 296
BfoI RGCGCY 2 cut(s) 305, 634
BglII AGATCT 1 cut(s) 1114
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 4 cut(s) 93, 594, 640, 741
Bme18I GGWCC 3 cut(s) 254, 343, 737
BmeT110I CYCGRG 1 cut(s) 1179
BmgT120I GGNCC 3 cut(s) 254, 343, 737
BmiI GGNNCC 5 cut(s) 267, 303, 344, 670, 703
BmrFI CCNGG 4 cut(s) 93, 594, 640, 741
BmrI ACTGGG 1 cut(s) 929
BmsI GCATC 1 cut(s) 166
BmuI ACTGGG 1 cut(s) 929
Bpu10I CCTNAGC 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 1103
BpuMI CCSGG 2 cut(s) 594, 640
BsaHI GRCGYC 2 cut(s) 302, 792
BsaJI CCNNGG 3 cut(s) 888, 962, 1209
BsaWI WCCGGW 1 cut(s) 671
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 449, 746
Bse1I ACTGG 3 cut(s) 346, 388, 935
Bse3DI GCAATG 1 cut(s) 1015
BseBI CCWGG 2 cut(s) 93, 741
BseDI CCNNGG 3 cut(s) 888, 962, 1209
BseGI GGATG 2 cut(s) 50, 511
BseLI CCNNNNNNNGG 3 cut(s) 122, 449, 746
BseMI GCAATG 1 cut(s) 1015
BseMII CTCAG 2 cut(s) 69, 1094
BseNI ACTGG 3 cut(s) 346, 388, 935
BseXI GCAGC 1 cut(s) 197
BsgI GTGCAG 1 cut(s) 204
BshNI GGYRCC 2 cut(s) 301, 701
BsiHKCI CYCGRG 1 cut(s) 1179
BsiSI CCGG 3 cut(s) 593, 639, 672
BslFI GGGAC 2 cut(s) 235, 1048
BslI CCNNNNNNNGG 3 cut(s) 122, 449, 746
BsmAI GTCTC 2 cut(s) 669, 770
BsmBI CGTCTC 1 cut(s) 669
BsmFI GGGAC 2 cut(s) 235, 1048
BsoBI CYCGRG 1 cut(s) 1179
Bsp143I GATC 3 cut(s) 1002, 1090, 1114
Bsp19I CCATGG 1 cut(s) 962
BspACI CCGC 3 cut(s) 885, 1049, 1190
BspCNI CTCAG 2 cut(s) 68, 1095
BspLI GGNNCC 5 cut(s) 267, 303, 344, 670, 703
BspMAI CTGCAG 1 cut(s) 300
BspT107I GGYRCC 2 cut(s) 301, 701
BsrDI GCAATG 1 cut(s) 1015
BsrI ACTGG 3 cut(s) 346, 388, 935
BssECI CCNNGG 3 cut(s) 888, 962, 1209
BssMI GATC 3 cut(s) 1002, 1090, 1114
BssNI GRCGYC 2 cut(s) 302, 792
BssT1I CCWWGG 2 cut(s) 962, 1209
Bst2UI CCWGG 2 cut(s) 93, 741
Bst4CI ACNGT 5 cut(s) 81, 277, 373, 449, 718
BstACI GRCGYC 2 cut(s) 302, 792
BstC8I GCNNGC 2 cut(s) 300, 1082
BstDEI CTNAG 3 cut(s) 55, 824, 1103
BstDSI CCRYGG 1 cut(s) 962
BstF5I GGATG 2 cut(s) 50, 511
BstH2I RGCGCY 2 cut(s) 305, 634
BstHHI GCGC 3 cut(s) 304, 633, 1073
BstKTI GATC 3 cut(s) 1005, 1093, 1117
BstMAI GTCTC 2 cut(s) 669, 770
BstMBI GATC 3 cut(s) 1002, 1090, 1114
BstMWI GCNNNNNNNGC 6 cut(s) 185, 323, 884, 1014, 1077, 1211
BstNI CCWGG 2 cut(s) 93, 741
BstNSI RCATGY 1 cut(s) 578
BstSCI CCNGG 4 cut(s) 91, 592, 638, 739
BstSFI CTRYAG 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 1114
BstYI RGATCY 1 cut(s) 1114
BtgI CCRYGG 1 cut(s) 962
BtsCI GGATG 2 cut(s) 50, 511
BtsIMutI CAGTG 4 cut(s) 369, 802, 833, 977
Cac8I GCNNGC 2 cut(s) 300, 1082
CaiI CAGNNNCTG 1 cut(s) 383
CfoI GCGC 3 cut(s) 304, 633, 1073
Cfr13I GGNCC 3 cut(s) 254, 343, 737
CsiI ACCWGGT 1 cut(s) 739
Csp6I GTAC 5 cut(s) 381, 702, 775, 819, 1100
CspCI CAANNNNNGTGG 2 cut(s) 1108, 1143
CviAII CATG 4 cut(s) 575, 658, 746, 963
CviQI GTAC 5 cut(s) 381, 702, 775, 819, 1100
DdeI CTNAG 3 cut(s) 55, 824, 1103
DinI GGCGCC 1 cut(s) 303
DpnI GATC 3 cut(s) 1004, 1092, 1116
DpnII GATC 3 cut(s) 1002, 1090, 1114
Eco130I CCWWGG 2 cut(s) 962, 1209
Eco47I GGWCC 3 cut(s) 254, 343, 737
Eco88I CYCGRG 1 cut(s) 1179
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 91, 739
EcoT14I CCWWGG 2 cut(s) 962, 1209
EgeI GGCGCC 1 cut(s) 303
EheI GGCGCC 1 cut(s) 303
ErhI CCWWGG 2 cut(s) 962, 1209
Esp3I CGTCTC 1 cut(s) 669
FaeI CATG 4 cut(s) 578, 661, 749, 966
FalI AAGNNNNNCTT 2 cut(s) 1125, 1157
FaqI GGGAC 2 cut(s) 235, 1048
FatI CATG 4 cut(s) 574, 657, 745, 962
FauNDI CATATG 2 cut(s) 61, 624
FbaI TGATCA 1 cut(s) 1090
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 2 cut(s) 37, 518
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 2 cut(s) 17, 458
GlaI GCGC 3 cut(s) 303, 632, 1072
GluI GCNGC 1 cut(s) 186
HaeII RGCGCY 2 cut(s) 305, 634
HapII CCGG 3 cut(s) 593, 639, 672
HhaI GCGC 3 cut(s) 304, 633, 1073
Hin1I GRCGYC 2 cut(s) 302, 792
Hin1II CATG 4 cut(s) 578, 661, 749, 966
Hin6I GCGC 3 cut(s) 302, 631, 1071
HinP1I GCGC 3 cut(s) 302, 631, 1071
HinfI GANTC 5 cut(s) 72, 474, 801, 849, 985
HpaII CCGG 3 cut(s) 593, 639, 672
HphI GGTGA 3 cut(s) 107, 571, 707
Hpy166II GTNNAC 3 cut(s) 557, 737, 800
Hpy188I TCNGA 5 cut(s) 117, 668, 761, 770, 892
Hpy188III TCNNGA 5 cut(s) 22, 853, 904, 1000, 1021
Hpy8I GTNNAC 3 cut(s) 557, 737, 800
HpyAV CCTTC 1 cut(s) 765
HpyCH4III ACNGT 5 cut(s) 81, 277, 373, 449, 718
HpyCH4IV ACGT 2 cut(s) 792, 1161
HpyCH4V TGCA 8 cut(s) 65, 185, 298, 432, 684, 1084, 1157, 1165
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 323, 884, 1014, 1077, 1211
HpyF3I CTNAG 3 cut(s) 55, 824, 1103
HpySE526I ACGT 2 cut(s) 792, 1161
Hsp92I GRCGYC 2 cut(s) 302, 792
Hsp92II CATG 4 cut(s) 578, 661, 749, 966
HspAI GCGC 3 cut(s) 302, 631, 1071
KasI GGCGCC 1 cut(s) 301
KpnI GGTACC 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 1090
Kzo9I GATC 3 cut(s) 1002, 1090, 1114
LmnI GCTCC 3 cut(s) 94, 239, 271
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 1 cut(s) 166
MabI ACCWGGT 1 cut(s) 739
MaeI CTAG 2 cut(s) 17, 458
MaeII ACGT 2 cut(s) 792, 1161
MaeIII GTNAC 5 cut(s) 95, 106, 508, 577, 1106
MalI GATC 3 cut(s) 1004, 1092, 1116
MboI GATC 3 cut(s) 1002, 1090, 1114
MboII GAAGA 2 cut(s) 797, 1072
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 1114
MluCI AATT 6 cut(s) 309, 320, 335, 409, 490, 529
Mly113I GGCGCC 1 cut(s) 302
MlyI GAGTC 2 cut(s) 81, 795
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 8 cut(s) 64, 349, 357, 446, 898, 950, 1045, 1203
MseI TTAA 4 cut(s) 465, 519, 645, 1124
MslI CAYNNNNRTG 1 cut(s) 467
MspI CCGG 3 cut(s) 593, 639, 672
MspR9I CCNGG 4 cut(s) 93, 594, 640, 741
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 2 cut(s) 93, 741
MwoI GCNNNNNNNGC 6 cut(s) 185, 323, 884, 1014, 1077, 1211
NarI GGCGCC 1 cut(s) 302
NciI CCSGG 2 cut(s) 594, 640
NcoI CCATGG 1 cut(s) 962
NdeI CATATG 2 cut(s) 61, 624
NdeII GATC 3 cut(s) 1002, 1090, 1114
NlaIII CATG 4 cut(s) 578, 661, 749, 966
NlaIV GGNNCC 5 cut(s) 267, 303, 344, 670, 703
NmuCI GTSAC 5 cut(s) 95, 106, 508, 577, 1106
NspI RCATGY 1 cut(s) 578
PaeR7I CTCGAG 1 cut(s) 1179
PciI ACATGT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 774
PfeI GAWTC 3 cut(s) 474, 849, 985
PflFI GACNNNGTC 1 cut(s) 741
PflMI CCANNNNNTGG 2 cut(s) 449, 746
PkrI GCNGC 1 cut(s) 187
PleI GAGTC 2 cut(s) 80, 795
PluTI GGCGCC 1 cut(s) 305
PpsI GAGTC 2 cut(s) 80, 795
PpuMI RGGWCCY 1 cut(s) 254
PscI ACATGT 1 cut(s) 574
PshBI ATTAAT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 91, 739
PspGI CCWGG 2 cut(s) 91, 739
PspN4I GGNNCC 5 cut(s) 267, 303, 344, 670, 703
PspPI GGNCC 3 cut(s) 254, 343, 737
PspPPI RGGWCCY 1 cut(s) 254
PspXI VCTCGAGB 1 cut(s) 1179
PstI CTGCAG 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 1 cut(s) 1114
PsyI GACNNNGTC 1 cut(s) 741
RsaI GTAC 5 cut(s) 382, 703, 776, 820, 1101
RsaNI GTAC 5 cut(s) 381, 702, 775, 819, 1100
RseI CAYNNNNRTG 1 cut(s) 467
SaqAI TTAA 4 cut(s) 465, 519, 645, 1124
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 3 cut(s) 1002, 1090, 1114
Sau96I GGNCC 3 cut(s) 254, 343, 737
SchI GAGTC 2 cut(s) 81, 795
ScrFI CCNGG 4 cut(s) 93, 594, 640, 741
SexAI ACCWGGT 1 cut(s) 739
SfaNI GCATC 1 cut(s) 166
SfcI CTRYAG 1 cut(s) 296
SfoI GGCGCC 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 1179
SinI GGWCC 3 cut(s) 254, 343, 737
SlaI CTCGAG 1 cut(s) 1179
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 2 cut(s) 1118, 1179
SmoI CTYRAG 2 cut(s) 1118, 1179
Sse9I AATT 6 cut(s) 309, 320, 335, 409, 490, 529
SsiI CCGC 3 cut(s) 885, 1049, 1190
SspDI GGCGCC 1 cut(s) 301
SspI AATATT 2 cut(s) 544, 808
SspMI CTAG 2 cut(s) 17, 458
StyD4I CCNGG 4 cut(s) 91, 592, 638, 739
StyI CCWWGG 2 cut(s) 962, 1209
TaaI ACNGT 5 cut(s) 81, 277, 373, 449, 718
TaiI ACGT 2 cut(s) 795, 1164
TaqI TCGA 3 cut(s) 477, 903, 1180
TasI AATT 6 cut(s) 309, 320, 335, 409, 490, 529
TfiI GAWTC 3 cut(s) 474, 849, 985
Tru1I TTAA 4 cut(s) 465, 519, 645, 1124
Tru9I TTAA 4 cut(s) 465, 519, 645, 1124
TscAI CASTG 4 cut(s) 376, 802, 840, 977
TseFI GTSAC 5 cut(s) 95, 106, 508, 577, 1106
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 5 cut(s) 95, 106, 508, 577, 1106
TspDTI ATGAA 4 cut(s) 483, 674, 798, 932
TspGWI ACGGA 1 cut(s) 668
TspRI CASTG 4 cut(s) 376, 802, 840, 977
Tth111I GACNNNGTC 1 cut(s) 741
Van91I CCANNNNNTGG 2 cut(s) 449, 746
VpaK11BI GGWCC 3 cut(s) 254, 343, 737
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 4 cut(s) 335, 409, 490, 529
XceI RCATGY 1 cut(s) 578
XhoI CTCGAG 1 cut(s) 1179
XspI CTAG 2 cut(s) 17, 458
ZraI GACGTC 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.