FvH4_6g41740

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000434
Physical Location & Seq
Forward (+)
32733723 .. 32735644
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41740.t1

Sequence Viewer

Length: 1185 bp
ATGGGTCACAATCTAGTCATGAGTATTTTTCCAATATTTTTATTATTATCCATAGCATATGCAGACTTCGATGTGACAAGTTCGAAGTATGGTGGAAAGCCTAATTCTGATATTAGTCAGCCTTTGGCAAAGGCTTGGAGTGATGCTTGTGCATCAACGTCGCCCAGTAGAATCATTGTTCCAAAAGGGACATTCCAACTAAAAGGAGCATCTTTCAAAGGTCCTTGCAAAGGTCCGATTGAGTTTCAGGTTGATGGCACATTGCAGGCGCCCCAAGATGGTGCGCAACTTATCAAGGCAGATACTTGGATCGAGTTCGCAAACCTTGAGAGGCTCACTCTAATGGGTACTGGAACCTTTGATGGTCAAGGACAAAAAGCTTGGAAAGAAAACGACTGCAACAAAAATCCGAAGTGTTCATCTATTGCCATTAATTTGAGGTTCAACTCTGTCAAACATTCATTAGTTAAGGATGTAACTTCGCTTAATAGCAAAAACTTCCACGTTAATGTTTTAGGATGTGAGCAACTTACATTCCAACATTTCACCGTCAAAGCACCTGGAGATAGTATTAACACAGATGGAATTCATATCGGACGCTCAACCGGGATTAACATTACCGACACGAACATTGGAACCGGTGACGATTGCATATCAATTGGTGATGGTACCAAGCAACTAACAATAAACAAAGTAACTTGTGGACCAGGTCATGGAATAAGCATCGGAAGTCTTGGAAGGTACAACAACGAAGACCACGTTACTGGAATCAATGTTAAGGACTGTACCATTAGTAATACAATGAATGGTGTTAGAATCAAGACATGGCCTAATTCTCCTATAGCTACCACCGCCTCAGATATTCACTTTGAGCACATCACAATGAATAATGTTGGTAACCCAATCCTCATAGACCAAGAATACTGCCCATATGGGCAGTGTAATACACAGACTCCATCAAAAGTCAAGATCAGCAATGTGAGCTTCAAGAATATTAGAGGGACAACTACAACTGCAGAGGCCGTGAAGATTGTATGTGCTAAAGGCTTGCCCTGTGACAAAGTGGTACTGAGTGACATAGATCTCAAGTTAACTGGCAAAGGCACTCTTACATCTCAATGCGCAAACGTGCAGCCCACGATTACTCAAGTGCCACAGGCTCTTGCTTGTGCTACCAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

395

Amino Acids

42.22

Weight (kDa)

8.45

Isoelectric Point (pI)

25.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 50 - 378 3.3e-104 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 285, 1123
Acc65I GGTACC 1 cut(s) 668
AccB1I GGYRCC 2 cut(s) 268, 668
AccB7I CCANNNNNTGG 1 cut(s) 713
AciI CCGC 1 cut(s) 852
AclWI GGATC 1 cut(s) 317
AcsI RAATTY 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 269
AfaI GTAC 5 cut(s) 349, 670, 743, 787, 1068
AfiI CCNNNNNNNGG 3 cut(s) 230, 278, 713
AgeI ACCGGT 1 cut(s) 638
AgsI TTSAA 3 cut(s) 217, 445, 988
AjnI CCWGG 2 cut(s) 559, 706
AluBI AGCT 3 cut(s) 380, 845, 984
AluI AGCT 3 cut(s) 380, 845, 984
Alw21I GWGCWC 1 cut(s) 876
AlwI GGATC 1 cut(s) 317
AoxI GGCC 2 cut(s) 827, 1020
ApeKI GCWGC 1 cut(s) 1132
ApoI RAATTY 1 cut(s) 585
AseI ATTAAT 1 cut(s) 432
AsiGI ACCGGT 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 668
AspLEI GCGC 3 cut(s) 271, 286, 1124
AspS9I GGNCC 3 cut(s) 221, 233, 704
AsuC2I CCSGG 1 cut(s) 607
AsuHPI GGTGA 3 cut(s) 538, 653, 674
AsuII TTCGAA 1 cut(s) 83
AvaII GGWCC 3 cut(s) 221, 233, 704
BanI GGYRCC 2 cut(s) 268, 668
BbsI GAAGAC 1 cut(s) 759
Bbv12I GWGCWC 1 cut(s) 876
BbvI GCAGC 1 cut(s) 1144
BccI CCATC 6 cut(s) 248, 272, 356, 575, 659, 964
BceAI ACGGC 1 cut(s) 1007
BcgI CGANNNNNNTGC 2 cut(s) 141, 175
BciT130I CCWGG 2 cut(s) 561, 708
BcnI CCSGG 1 cut(s) 607
BfaI CTAG 1 cut(s) 14
BfmI CTRYAG 2 cut(s) 840, 1014
BfoI RGCGCY 1 cut(s) 272
BglII AGATCT 1 cut(s) 1081
BisI GCNGC 1 cut(s) 1133
BlsI GCNGC 1 cut(s) 1134
Bme1390I CCNGG 3 cut(s) 561, 607, 708
Bme18I GGWCC 3 cut(s) 221, 233, 704
BmgT120I GGNCC 3 cut(s) 221, 233, 704
BmiI GGNNCC 4 cut(s) 270, 355, 637, 670
BmrFI CCNGG 3 cut(s) 561, 607, 708
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 4 cut(s) 133, 161, 218, 732
BmuI ACTGGG 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 759
BplI GAGNNNNNCTC 2 cut(s) 322, 354
BpmI CTGGAG 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 83
BpuEI CTTGAG 3 cut(s) 347, 1070, 1131
BpuMI CCSGG 1 cut(s) 607
BsaHI GRCGYC 1 cut(s) 269
BsaWI WCCGGW 1 cut(s) 638
BsaXI ACNNNNNCTCC 2 cut(s) 937, 967
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 278, 713
Bse118I RCCGGY 1 cut(s) 638
Bse1I ACTGG 4 cut(s) 165, 355, 769, 1099
Bse3DI GCAATG 2 cut(s) 260, 982
BseBI CCWGG 2 cut(s) 561, 708
BseGI GGATG 2 cut(s) 478, 524
BseLI CCNNNNNNNGG 3 cut(s) 230, 278, 713
BseMI GCAATG 2 cut(s) 260, 982
BseMII CTCAG 2 cut(s) 870, 1061
BseNI ACTGG 4 cut(s) 165, 355, 769, 1099
BseXI GCAGC 1 cut(s) 1144
BsgI GTGCAG 1 cut(s) 1151
BshFI GGCC 2 cut(s) 829, 1022
BshNI GGYRCC 2 cut(s) 268, 668
BshTI ACCGGT 1 cut(s) 638
BsiHKAI GWGCWC 1 cut(s) 876
BsiSI CCGG 2 cut(s) 606, 639
BslFI GGGAC 2 cut(s) 202, 1015
BslI CCNNNNNNNGG 3 cut(s) 230, 278, 713
BsmFI GGGAC 2 cut(s) 202, 1015
BsnI GGCC 2 cut(s) 829, 1022
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 876
Bsp143I GATC 3 cut(s) 309, 969, 1081
BspACI CCGC 1 cut(s) 852
BspANI GGCC 2 cut(s) 829, 1022
BspCNI CTCAG 2 cut(s) 869, 1062
BspHI TCATGA 1 cut(s) 18
BspLI GGNNCC 4 cut(s) 270, 355, 637, 670
BspMAI CTGCAG 1 cut(s) 1018
BspPI GGATC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 83
BspT107I GGYRCC 2 cut(s) 268, 668
BsrDI GCAATG 2 cut(s) 260, 982
BsrFI RCCGGY 1 cut(s) 638
BsrI ACTGG 4 cut(s) 165, 355, 769, 1099
BssAI RCCGGY 1 cut(s) 638
BssMI GATC 3 cut(s) 309, 969, 1081
BssNI GRCGYC 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 561, 708
Bst4CI ACNGT 2 cut(s) 550, 785
BstACI GRCGYC 1 cut(s) 269
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 267, 1049
BstDEI CTNAG 2 cut(s) 856, 1070
BstEII GGTNACC 1 cut(s) 896
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 2 cut(s) 478, 524
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 3 cut(s) 271, 286, 1124
BstKTI GATC 3 cut(s) 312, 972, 1084
BstMBI GATC 3 cut(s) 309, 969, 1081
BstMWI GCNNNNNNNGC 2 cut(s) 851, 981
BstNI CCWGG 2 cut(s) 561, 708
BstPI GGTNACC 1 cut(s) 896
BstSCI CCNGG 3 cut(s) 559, 605, 706
BstSFI CTRYAG 2 cut(s) 840, 1014
BstV1I GCAGC 1 cut(s) 1144
BstV2I GAAGAC 1 cut(s) 759
BstX2I RGATCY 1 cut(s) 1081
BstXI CCANNNNNNTGG 1 cut(s) 764
BstYI RGATCY 1 cut(s) 1081
BsuRI GGCC 2 cut(s) 829, 1022
BtsCI GGATG 2 cut(s) 478, 524
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 1 cut(s) 944
Cac8I GCNNGC 2 cut(s) 267, 1049
CciI TCATGA 1 cut(s) 18
CfoI GCGC 3 cut(s) 271, 286, 1124
Cfr10I RCCGGY 1 cut(s) 638
Cfr13I GGNCC 3 cut(s) 221, 233, 704
CseI GACGC 1 cut(s) 606
CsiI ACCWGGT 1 cut(s) 706
Csp6I GTAC 5 cut(s) 348, 669, 742, 786, 1067
CspAI ACCGGT 1 cut(s) 638
CviAII CATG 3 cut(s) 19, 713, 825
CviQI GTAC 5 cut(s) 348, 669, 742, 786, 1067
DdeI CTNAG 2 cut(s) 856, 1070
DinI GGCGCC 1 cut(s) 270
DpnI GATC 3 cut(s) 311, 971, 1083
DpnII GATC 3 cut(s) 309, 969, 1081
Eco47I GGWCC 3 cut(s) 221, 233, 704
Eco91I GGTNACC 1 cut(s) 896
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 221
EcoO65I GGTNACC 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 585
EcoRII CCWGG 2 cut(s) 559, 706
EgeI GGCGCC 1 cut(s) 270
EheI GGCGCC 1 cut(s) 270
FaeI CATG 3 cut(s) 22, 716, 828
FalI AAGNNNNNCTT 2 cut(s) 1092, 1124
FaqI GGGAC 2 cut(s) 202, 1015
FatI CATG 3 cut(s) 18, 712, 824
FauNDI CATATG 2 cut(s) 58, 931
Fnu4HI GCNGC 1 cut(s) 1133
FokI GGATG 2 cut(s) 485, 531
Fsp4HI GCNGC 1 cut(s) 1133
FspBI CTAG 1 cut(s) 14
FspI TGCGCA 2 cut(s) 285, 1123
GlaI GCGC 3 cut(s) 270, 285, 1123
GluI GCNGC 1 cut(s) 1133
GsuI CTGGAG 1 cut(s) 582
HaeII RGCGCY 1 cut(s) 272
HaeIII GGCC 2 cut(s) 829, 1022
HapII CCGG 2 cut(s) 606, 639
HgaI GACGC 1 cut(s) 606
HhaI GCGC 3 cut(s) 271, 286, 1124
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 3 cut(s) 22, 716, 828
Hin6I GCGC 3 cut(s) 269, 284, 1122
HinP1I GCGC 3 cut(s) 269, 284, 1122
HincII GTYRAC 1 cut(s) 1092
HindII GTYRAC 1 cut(s) 1092
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 4 cut(s) 171, 768, 816, 952
HpaI GTTAAC 1 cut(s) 1092
HpaII CCGG 2 cut(s) 606, 639
HphI GGTGA 3 cut(s) 538, 653, 674
Hpy166II GTNNAC 2 cut(s) 704, 1092
Hpy188I TCNGA 6 cut(s) 109, 237, 411, 596, 728, 859
Hpy188III TCNNGA 4 cut(s) 19, 820, 967, 988
Hpy8I GTNNAC 2 cut(s) 704, 1092
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 1 cut(s) 732
HpyCH4III ACNGT 2 cut(s) 550, 785
HpyCH4IV ACGT 4 cut(s) 158, 504, 759, 1128
HpyCH4V TGCA 8 cut(s) 62, 152, 228, 265, 399, 651, 1016, 1132
HpyF10VI GCNNNNNNNGC 2 cut(s) 851, 981
HpyF3I CTNAG 2 cut(s) 856, 1070
HpySE526I ACGT 4 cut(s) 158, 504, 759, 1128
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 3 cut(s) 22, 716, 828
HspAI GCGC 3 cut(s) 269, 284, 1122
KasI GGCGCC 1 cut(s) 268
KpnI GGTACC 1 cut(s) 672
KspAI GTTAAC 1 cut(s) 1092
Kzo9I GATC 3 cut(s) 309, 969, 1081
LmnI GCTCC 1 cut(s) 206
Lsp1109I GCAGC 1 cut(s) 1144
LweI GCATC 4 cut(s) 133, 161, 218, 732
MabI ACCWGGT 1 cut(s) 706
MaeI CTAG 1 cut(s) 14
MaeII ACGT 4 cut(s) 158, 504, 759, 1128
MaeIII GTNAC 9 cut(s) 5, 73, 475, 641, 694, 760, 896, 1055, 1073
MalI GATC 3 cut(s) 311, 971, 1083
MboI GATC 3 cut(s) 309, 969, 1081
MboII GAAGA 2 cut(s) 764, 1039
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 1 cut(s) 1081
MhlI GDGCHC 1 cut(s) 876
MluCI AATT 5 cut(s) 103, 433, 585, 657, 832
Mly113I GGCGCC 1 cut(s) 269
MlyI GAGTC 1 cut(s) 946
MmeI TCCRAC 2 cut(s) 220, 562
MnlI CCTC 6 cut(s) 324, 432, 865, 917, 992, 1012
MseI TTAA 8 cut(s) 432, 468, 486, 507, 573, 612, 777, 1091
MslI CAYNNNNRTG 4 cut(s) 341, 507, 881, 1117
MspI CCGG 2 cut(s) 606, 639
MspR9I CCNGG 3 cut(s) 561, 607, 708
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 2 cut(s) 561, 708
MwoI GCNNNNNNNGC 2 cut(s) 851, 981
NarI GGCGCC 1 cut(s) 269
NciI CCSGG 1 cut(s) 607
NdeI CATATG 2 cut(s) 58, 931
NdeII GATC 3 cut(s) 309, 969, 1081
NlaIII CATG 3 cut(s) 22, 716, 828
NlaIV GGNNCC 4 cut(s) 270, 355, 637, 670
NmuCI GTSAC 5 cut(s) 5, 73, 641, 1055, 1073
NsbI TGCGCA 2 cut(s) 285, 1123
NspV TTCGAA 1 cut(s) 83
PagI TCATGA 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 756
PfeI GAWTC 3 cut(s) 171, 768, 816
PflFI GACNNNGTC 1 cut(s) 708
PflMI CCANNNNNTGG 1 cut(s) 713
PinAI ACCGGT 1 cut(s) 638
PkrI GCNGC 1 cut(s) 1134
PleI GAGTC 1 cut(s) 946
PluTI GGCGCC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 946
PpuMI RGGWCCY 1 cut(s) 221
PshBI ATTAAT 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 2 cut(s) 559, 706
PspEI GGTNACC 1 cut(s) 896
PspGI CCWGG 2 cut(s) 559, 706
PspN4I GGNNCC 4 cut(s) 270, 355, 637, 670
PspPI GGNCC 3 cut(s) 221, 233, 704
PspPPI RGGWCCY 1 cut(s) 221
PstI CTGCAG 1 cut(s) 1018
PsuI RGATCY 1 cut(s) 1081
PsyI GACNNNGTC 1 cut(s) 708
RsaI GTAC 5 cut(s) 349, 670, 743, 787, 1068
RsaNI GTAC 5 cut(s) 348, 669, 742, 786, 1067
RseI CAYNNNNRTG 4 cut(s) 341, 507, 881, 1117
SaqAI TTAA 8 cut(s) 432, 468, 486, 507, 573, 612, 777, 1091
SatI GCNGC 1 cut(s) 1133
Sau3AI GATC 3 cut(s) 309, 969, 1081
Sau96I GGNCC 3 cut(s) 221, 233, 704
SchI GAGTC 1 cut(s) 946
ScrFI CCNGG 3 cut(s) 561, 607, 708
SduI GDGCHC 1 cut(s) 876
SexAI ACCWGGT 1 cut(s) 706
SfaNI GCATC 4 cut(s) 133, 161, 218, 732
SfcI CTRYAG 2 cut(s) 840, 1014
SfoI GGCGCC 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 83
SinI GGWCC 3 cut(s) 221, 233, 704
SmiMI CAYNNNNRTG 4 cut(s) 341, 507, 881, 1117
SmlI CTYRAG 3 cut(s) 326, 1085, 1146
SmoI CTYRAG 3 cut(s) 326, 1085, 1146
Sse9I AATT 5 cut(s) 103, 433, 585, 657, 832
SsiI CCGC 1 cut(s) 852
SspDI GGCGCC 1 cut(s) 268
SspI AATATT 2 cut(s) 36, 994
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 559, 605, 706
TaaI ACNGT 2 cut(s) 550, 785
TaiI ACGT 4 cut(s) 161, 507, 762, 1131
TaqI TCGA 3 cut(s) 69, 83, 312
TasI AATT 5 cut(s) 103, 433, 585, 657, 832
TfiI GAWTC 3 cut(s) 171, 768, 816
Tru1I TTAA 8 cut(s) 432, 468, 486, 507, 573, 612, 777, 1091
Tru9I TTAA 8 cut(s) 432, 468, 486, 507, 573, 612, 777, 1091
TscAI CASTG 1 cut(s) 944
TseFI GTSAC 5 cut(s) 5, 73, 641, 1055, 1073
TseI GCWGC 1 cut(s) 1132
Tsp45I GTSAC 5 cut(s) 5, 73, 641, 1055, 1073
TspDTI ATGAA 5 cut(s) 408, 450, 578, 818, 899
TspRI CASTG 1 cut(s) 944
Tth111I GACNNNGTC 1 cut(s) 708
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 3 cut(s) 221, 233, 704
VspI ATTAAT 1 cut(s) 432
XagI CCTNNNNNAGG 1 cut(s) 228
XapI RAATTY 1 cut(s) 585
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.