Rorug05G0520000

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000434
Physical Location & Seq
Forward (+)
70427126 .. 70431695
4570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0520000.1

Sequence Viewer

Length: 1731 bp
ATGATGGGCTCTGAGAACCACCAGGACCATCAATTCGGTGGTTCGAAAGAAGAGATGGAGAGTCTCGTCCTCGACGACCCGCCGCCGTTGAACAACGGCGGCGCCAGTAAGTCGTCGTATTCGAATTACCGCAGCGCCATGTCGACGCTCTCCGACACGCACCACCCGCTCTCGTCTATAATCGTCTCCACGACCCCGGCCCCGGCCGATTCCGATCCGCTTCTGACTGCGCCACCGCCGTTCCGCGATCTCCGAAACCCTAACGGCGCCGACAGCTCCGCGTACGTCGACCCGCCCTCTTACGGCGACGTGGTTTTCAGTCCGTTGGATGGCGAGACGATCGGCGAAATCAACGGCGTCGAGAGTCCGAGCAAGAGCTCCGACCATTCCAGCTCTTATTCGCTTTCGCGATCTTCGTCTTCCAGCTCCGACTACATCAAAATCACGGTTTCGAATCCTCAGAAGGAGCAGGAGTCCACGAATTCGGTTATTCCCGGAGGGAATACTTACGTCACGTATCTGATCACGACGAGGACGAACATTCCGGAGTTCGACGGCTCGGAATTTGGCGTCCGGCGGCGGTTTCGGGACATTGTGACGTTATCGGACCGGCTGGCCGAGGCCTACAGAGGGTTCTTCATTCCGCCGCGGCCGGATAAGAACGTGGTGGAGAGCCAGGTGATGCAGAAACAAGAGTTTGTGGAGCAGAGAAGAGTGGCATTGGAGAAGTACTTGAGGCGGTTAGCGGCTCACCCTGTGATCAAGAAGAGTGATGAGTTGAAGGTGTTTCTGCAAGTTCAGGGGAGGTTTCCGTTGCCGATGACCACCGACGTGGCGTCGAGAATGCTGGATGGGGCTGCTAAGCTGCCGAAGCAGTTGTTCGGCGAGAGTTCCCCGGTGGCTCCACACGAGGTGGTTCAGCCAGCGAAAGGCGGGAGGGACTTGCTGAGGTTGTTCAAGGAGCTGAAGCAGTCTGTGGCCAATGACTGGGGAGGTTCGAAGCCGCTTGTGGGAGAGGAGGATAAGGAGTTTATGGAAAAGAAGGACCACATGCAGGAGCTCGAGCAACAGCTCAGCAATGCATCTCAGCATGCGGAATCACTTGTAAAAGCTCAACAAGACATGGGAGAGACTATGGGAGAGTTAGGACTAGCATTTATAAAACTGACTAAGTTTGAAAATGAGGAGGCCTTGTTCAACTCTCAAAGAATACGTGCTGCTGACATGAAGAATGTGGCGACTGCTGCTGTAAAAGCCAGCAGATACTATCGAGAATTGAATTCTCAGACTGTCAAACATTTGGACACATTCCATGAGTATCTTGGGCTAATGTTATCCGTCAAAGGTGCATTCTCGGATCGATCAAGTGCTTTATTGACTGTTCAGACTCTTCTATCAGAATTGGGTTCATTGCAGTCGAGGGCTGAAAAACTTGAAGTTGCATCAAACAAAATTTTTGGTGGTGACAAATCAAGGACTCGCAAACTAGAGGAGTTAAATGAAACCATAAGAGCTACTGAGGATGCTAAGAATATAGCAATCAGAGAATATGAGAGAATCAAGGAAAACAACAGAAGTGAACTTGAGAGGCTTGACAGCGAGAGGCATGCTGACTTCTTGAACATGTTGAAGGGGTTTGTTCATAATCAGGTGGGATATGCCGAGAAAATAGCAAATGTGTGGTCCAAGGTTGCCGAGGAGACCAGTTCGTATGCACGAGAGAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

576

Amino Acids

64.14

Weight (kDa)

5.69

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PX PF00787 183 - 266 2.9e-18 PX domain
Vps5 PF09325 334 - 566 2.5e-21 Vps5 C terminal like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1160
AccB1I GGYRCC 2 cut(s) 101, 266
AccB7I CCANNNNNTGG 1 cut(s) 987
AccBSI CCGCTC 1 cut(s) 169
AccI GTMKAC 2 cut(s) 143, 288
AccII CGCG 4 cut(s) 246, 281, 409, 649
AccIII TCCGGA 1 cut(s) 544
AclWI GGATC 2 cut(s) 209, 1365
AcoI YGGCCR 4 cut(s) 204, 615, 650, 978
AcsI RAATTY 4 cut(s) 481, 563, 1279, 1452
AcuI CTGAAG 1 cut(s) 986
AcyI GRCGYC 5 cut(s) 102, 267, 357, 570, 836
AdeI CACNNNGTG 2 cut(s) 757, 913
AfaI GTAC 2 cut(s) 284, 731
AfiI CCNNNNNNNGG 8 cut(s) 202, 302, 329, 630, 929, 987, 1010, 1054
AflIII ACRYGT 1 cut(s) 1623
AgsI TTSAA 9 cut(s) 91, 781, 958, 1178, 1198, 1279, 1436, 1621, 1630
AhdI GACNNNNNGTC 1 cut(s) 835
AjiI CACGTC 2 cut(s) 310, 832
AjnI CCWGG 2 cut(s) 21, 675
AleI CACNNNNGTG 1 cut(s) 830
AloI GAACNNNNNNTCC 2 cut(s) 1178, 1210
Alw21I GWGCWC 3 cut(s) 380, 1062, 1727
Alw26I GTCTC 5 cut(s) 68, 190, 329, 1124, 1694
AlwI GGATC 2 cut(s) 209, 1365
Ama87I CYCGRG 1 cut(s) 1061
Aor13HI TCCGGA 1 cut(s) 544
AoxI GGCC 7 cut(s) 198, 204, 615, 621, 650, 978, 1188
ApeKI GCWGC 5 cut(s) 132, 857, 865, 1217, 1244
ApoI RAATTY 4 cut(s) 481, 563, 1279, 1452
ArsI GACNNNNNNTTYG 4 cut(s) 17, 49, 1667, 1699
AspLEI GCGC 4 cut(s) 104, 137, 232, 269
AspS9I GGNCC 5 cut(s) 25, 199, 607, 1045, 1683
AsuC2I CCSGG 4 cut(s) 197, 203, 495, 896
AsuHPI GGTGA 3 cut(s) 691, 743, 1475
AsuII TTCGAA 4 cut(s) 44, 122, 452, 998
AvaI CYCGRG 1 cut(s) 1061
AvaII GGWCC 4 cut(s) 25, 607, 1045, 1683
BalI TGGCCA 1 cut(s) 980
BanI GGYRCC 2 cut(s) 101, 266
BanII GRGCYC 4 cut(s) 11, 380, 1062, 1727
BauI CACGAG 2 cut(s) 908, 1716
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 3 cut(s) 380, 1062, 1727
BbvCI CCTCAGC 1 cut(s) 947
BbvI GCAGC 5 cut(s) 144, 844, 852, 1204, 1231
BccI CCATC 4 cut(s) 36, 49, 323, 845
BceAI ACGGC 7 cut(s) 70, 112, 223, 280, 319, 370, 571
BcgI CGANNNNNNTGC 2 cut(s) 1589, 1623
BciT130I CCWGG 2 cut(s) 23, 677
BclI TGATCA 2 cut(s) 522, 759
BcnI CCSGG 4 cut(s) 197, 203, 495, 896
BcoDI GTCTC 5 cut(s) 68, 190, 329, 1124, 1694
BfaI CTAG 2 cut(s) 1151, 1487
BfmI CTRYAG 1 cut(s) 625
BfoI RGCGCY 3 cut(s) 105, 138, 270
BlpI GCTNAGC 2 cut(s) 861, 1073
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 6 cut(s) 23, 197, 203, 495, 677, 896
Bme18I GGWCC 4 cut(s) 25, 607, 1045, 1683
BmeRI GACNNNNNGTC 1 cut(s) 835
BmeT110I CYCGRG 1 cut(s) 1061
BmgBI CACGTC 2 cut(s) 310, 832
BmgT120I GGNCC 5 cut(s) 25, 199, 607, 1045, 1683
BmiI GGNNCC 4 cut(s) 103, 201, 268, 903
BmrFI CCNGG 6 cut(s) 23, 197, 203, 495, 677, 896
BmrI ACTGGG 1 cut(s) 997
BmsI GCATC 4 cut(s) 672, 1091, 1451, 1513
BmuI ACTGGG 1 cut(s) 997
BpiI GAAGAC 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 947
Bpu1102I GCTNAGC 2 cut(s) 861, 1073
Bpu14I TTCGAA 4 cut(s) 44, 122, 452, 998
BpuEI CTTGAG 2 cut(s) 754, 1604
BpuMI CCSGG 4 cut(s) 197, 203, 495, 896
Bsa29I ATCGAT 1 cut(s) 1360
BsaAI YACGTR 2 cut(s) 516, 1214
BsaBI GATNNNNATC 1 cut(s) 213
BsaHI GRCGYC 5 cut(s) 102, 267, 357, 570, 836
BsaI GGTCTC 1 cut(s) 1694
BsaJI CCNNGG 7 cut(s) 195, 201, 618, 647, 894, 1686, 1695
BsaWI WCCGGW 1 cut(s) 544
BsaXI ACNNNNNCTCC 4 cut(s) 458, 488, 1178, 1208
Bsc4I CCNNNNNNNGG 8 cut(s) 202, 302, 329, 630, 929, 987, 1010, 1054
Bse118I RCCGGY 1 cut(s) 609
Bse1I ACTGG 3 cut(s) 105, 992, 1704
Bse3DI GCAATG 2 cut(s) 1084, 1409
Bse8I GATNNNNATC 1 cut(s) 213
BseAI TCCGGA 1 cut(s) 544
BseBI CCWGG 2 cut(s) 23, 677
BseCI ATCGAT 1 cut(s) 1360
BseDI CCNNGG 7 cut(s) 195, 201, 618, 647, 894, 1686, 1695
BseGI GGATG 3 cut(s) 334, 856, 1528
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 8 cut(s) 202, 302, 329, 630, 929, 987, 1010, 1054
BseMI GCAATG 2 cut(s) 1084, 1409
BseMII CTCAG 6 cut(s) 473, 938, 1087, 1100, 1298, 1509
BseNI ACTGG 3 cut(s) 105, 992, 1704
BseRI GAGGAG 4 cut(s) 1031, 1199, 1505, 1712
BseX3I CGGCCG 2 cut(s) 204, 650
BseXI GCAGC 5 cut(s) 144, 844, 852, 1204, 1231
Bsh1236I CGCG 4 cut(s) 246, 281, 409, 649
Bsh1285I CGRYCG 3 cut(s) 207, 342, 653
BshFI GGCC 7 cut(s) 200, 206, 617, 623, 652, 980, 1190
BshNI GGYRCC 2 cut(s) 101, 266
BshVI ATCGAT 1 cut(s) 1360
BsiEI CGRYCG 3 cut(s) 207, 342, 653
BsiHKAI GWGCWC 3 cut(s) 380, 1062, 1727
BsiHKCI CYCGRG 1 cut(s) 1061
BsiSI CCGG 8 cut(s) 197, 203, 495, 545, 574, 610, 653, 896
BsiWI CGTACG 1 cut(s) 282
BslFI GGGAC 2 cut(s) 602, 953
BslI CCNNNNNNNGG 8 cut(s) 202, 302, 329, 630, 929, 987, 1010, 1054
BsmAI GTCTC 5 cut(s) 68, 190, 329, 1124, 1694
BsmBI CGTCTC 2 cut(s) 190, 329
BsmFI GGGAC 2 cut(s) 602, 953
BsmI GAATGC 2 cut(s) 849, 1349
BsnI GGCC 7 cut(s) 200, 206, 617, 623, 652, 980, 1190
Bso31I GGTCTC 1 cut(s) 1694
BsoBI CYCGRG 1 cut(s) 1061
Bsp119I TTCGAA 4 cut(s) 44, 122, 452, 998
Bsp1286I GDGCHC 4 cut(s) 11, 380, 1062, 1727
Bsp13I TCCGGA 1 cut(s) 544
Bsp143I GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
Bsp1720I GCTNAGC 2 cut(s) 861, 1073
Bsp68I TCGCGA 1 cut(s) 409
BspANI GGCC 7 cut(s) 200, 206, 617, 623, 652, 980, 1190
BspCNI CTCAG 7 cut(s) 4, 472, 939, 1086, 1099, 1297, 1510
BspDI ATCGAT 1 cut(s) 1360
BspEI TCCGGA 1 cut(s) 544
BspFNI CGCG 4 cut(s) 246, 281, 409, 649
BspLI GGNNCC 4 cut(s) 103, 201, 268, 903
BspPI GGATC 2 cut(s) 209, 1365
BspT104I TTCGAA 4 cut(s) 44, 122, 452, 998
BspT107I GGYRCC 2 cut(s) 101, 266
BspTNI GGTCTC 1 cut(s) 1694
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 2 cut(s) 1084, 1409
BsrFI RCCGGY 1 cut(s) 609
BsrI ACTGG 3 cut(s) 105, 992, 1704
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 7 cut(s) 195, 201, 618, 647, 894, 1686, 1695
BssMI GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
BssNI GRCGYC 5 cut(s) 102, 267, 357, 570, 836
BssSI CACGAG 2 cut(s) 908, 1716
BssT1I CCWWGG 1 cut(s) 1686
Bst2BI CACGAG 2 cut(s) 908, 1716
Bst2UI CCWGG 2 cut(s) 23, 677
Bst4CI ACNGT 3 cut(s) 448, 1291, 1381
Bst6I CTCTTC 4 cut(s) 45, 706, 761, 1395
BstACI GRCGYC 5 cut(s) 102, 267, 357, 570, 836
BstBAI YACGTR 2 cut(s) 516, 1214
BstBI TTCGAA 4 cut(s) 44, 122, 452, 998
BstC8I GCNNGC 5 cut(s) 615, 924, 1092, 1258, 1608
BstDSI CCRYGG 1 cut(s) 647
BstF5I GGATG 3 cut(s) 334, 856, 1528
BstFNI CGCG 4 cut(s) 246, 281, 409, 649
BstH2I RGCGCY 3 cut(s) 105, 138, 270
BstHHI GCGC 4 cut(s) 104, 137, 232, 269
BstKTI GATC 8 cut(s) 217, 250, 342, 413, 525, 762, 1360, 1364
BstMAI GTCTC 5 cut(s) 68, 190, 329, 1124, 1694
BstMBI GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
BstMCI CGRYCG 3 cut(s) 207, 342, 653
BstMWI GCNNNNNNNGC 5 cut(s) 166, 273, 871, 1244, 1253
BstNI CCWGG 2 cut(s) 23, 677
BstNSI RCATGY 4 cut(s) 1054, 1094, 1610, 1627
BstSCI CCNGG 6 cut(s) 21, 195, 201, 493, 675, 894
BstSFI CTRYAG 1 cut(s) 625
BstUI CGCG 4 cut(s) 246, 281, 409, 649
BstV1I GCAGC 5 cut(s) 144, 844, 852, 1204, 1231
BstV2I GAAGAC 1 cut(s) 411
BstXI CCANNNNNNTGG 1 cut(s) 832
BstZI CGGCCG 2 cut(s) 204, 650
Bsu15I ATCGAT 1 cut(s) 1360
BsuRI GGCC 7 cut(s) 200, 206, 617, 623, 652, 980, 1190
BsuTUI ATCGAT 1 cut(s) 1360
BtgI CCRYGG 1 cut(s) 647
BtrI CACGTC 2 cut(s) 310, 832
BtsCI GGATG 3 cut(s) 334, 856, 1528
BtuMI TCGCGA 1 cut(s) 409
Cac8I GCNNGC 5 cut(s) 615, 924, 1092, 1258, 1608
CfoI GCGC 4 cut(s) 104, 137, 232, 269
Cfr10I RCCGGY 1 cut(s) 609
Cfr13I GGNCC 5 cut(s) 25, 199, 607, 1045, 1683
Cfr42I CCGCGG 1 cut(s) 650
ClaI ATCGAT 1 cut(s) 1360
CpoI CGGWCCG 1 cut(s) 607
CseI GACGC 4 cut(s) 154, 346, 559, 825
Csp6I GTAC 2 cut(s) 283, 730
CspI CGGWCCG 1 cut(s) 607
CviAII CATG 8 cut(s) 139, 1051, 1091, 1123, 1225, 1313, 1607, 1624
CviQI GTAC 2 cut(s) 283, 730
DinI GGCGCC 2 cut(s) 103, 268
DpnI GATC 8 cut(s) 216, 249, 341, 412, 524, 761, 1359, 1363
DpnII GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
DraIII CACNNNGTG 2 cut(s) 757, 913
DriI GACNNNNNGTC 1 cut(s) 835
EaeI YGGCCR 4 cut(s) 204, 615, 650, 978
EagI CGGCCG 2 cut(s) 204, 650
Eam1104I CTCTTC 4 cut(s) 45, 706, 761, 1395
Eam1105I GACNNNNNGTC 1 cut(s) 835
EarI CTCTTC 4 cut(s) 45, 706, 761, 1395
EciI GGCGGA 1 cut(s) 633
Ecl136II GAGCTC 3 cut(s) 378, 1060, 1725
EclXI CGGCCG 2 cut(s) 204, 650
Eco130I CCWWGG 1 cut(s) 1686
Eco147I AGGCCT 2 cut(s) 623, 1190
Eco24I GRGCYC 4 cut(s) 11, 380, 1062, 1727
Eco31I GGTCTC 1 cut(s) 1694
Eco47I GGWCC 4 cut(s) 25, 607, 1045, 1683
Eco52I CGGCCG 2 cut(s) 204, 650
Eco53kI GAGCTC 3 cut(s) 378, 1060, 1725
Eco57I CTGAAG 1 cut(s) 986
Eco88I CYCGRG 1 cut(s) 1061
EcoICRI GAGCTC 3 cut(s) 378, 1060, 1725
EcoRI GAATTC 2 cut(s) 481, 1279
EcoRII CCWGG 2 cut(s) 21, 675
EcoT14I CCWWGG 1 cut(s) 1686
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 4 cut(s) 11, 380, 1062, 1727
EgeI GGCGCC 2 cut(s) 103, 268
EheI GGCGCC 2 cut(s) 103, 268
ErhI CCWWGG 1 cut(s) 1686
Esp3I CGTCTC 2 cut(s) 190, 329
FaeI CATG 8 cut(s) 142, 1054, 1094, 1126, 1228, 1316, 1610, 1627
FaqI GGGAC 2 cut(s) 602, 953
FatI CATG 8 cut(s) 138, 1050, 1090, 1122, 1224, 1312, 1606, 1623
FauI CCCGC 4 cut(s) 87, 174, 300, 926
FbaI TGATCA 2 cut(s) 522, 759
FblI GTMKAC 2 cut(s) 143, 288
FokI GGATG 3 cut(s) 341, 863, 1535
FriOI GRGCYC 4 cut(s) 11, 380, 1062, 1727
FspBI CTAG 2 cut(s) 1151, 1487
GlaI GCGC 4 cut(s) 103, 136, 231, 268
HaeII RGCGCY 3 cut(s) 105, 138, 270
HaeIII GGCC 7 cut(s) 200, 206, 617, 623, 652, 980, 1190
HapII CCGG 8 cut(s) 197, 203, 495, 545, 574, 610, 653, 896
HgaI GACGC 4 cut(s) 154, 346, 559, 825
HhaI GCGC 4 cut(s) 104, 137, 232, 269
Hin1I GRCGYC 5 cut(s) 102, 267, 357, 570, 836
Hin1II CATG 8 cut(s) 142, 1054, 1094, 1126, 1228, 1316, 1610, 1627
Hin6I GCGC 4 cut(s) 102, 135, 230, 267
HinP1I GCGC 4 cut(s) 102, 135, 230, 267
HincII GTYRAC 2 cut(s) 144, 289
HindII GTYRAC 2 cut(s) 144, 289
HinfI GANTC 9 cut(s) 61, 209, 364, 454, 473, 1097, 1387, 1477, 1557
HpaII CCGG 8 cut(s) 197, 203, 495, 545, 574, 610, 653, 896
HphI GGTGA 3 cut(s) 691, 743, 1475
Hpy166II GTNNAC 4 cut(s) 144, 289, 477, 1580
Hpy8I GTNNAC 4 cut(s) 144, 289, 477, 1580
HpyAV CCTTC 4 cut(s) 457, 775, 1036, 1624
HpyCH4III ACNGT 3 cut(s) 448, 1291, 1381
HpyCH4IV ACGT 8 cut(s) 285, 309, 510, 515, 599, 663, 831, 1213
HpyCH4V TGCA 8 cut(s) 685, 793, 1054, 1082, 1349, 1414, 1442, 1715
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 273, 871, 1244, 1253
HpySE526I ACGT 8 cut(s) 285, 309, 510, 515, 599, 663, 831, 1213
Hsp92I GRCGYC 5 cut(s) 102, 267, 357, 570, 836
Hsp92II CATG 8 cut(s) 142, 1054, 1094, 1126, 1228, 1316, 1610, 1627
HspAI GCGC 4 cut(s) 102, 135, 230, 267
KasI GGCGCC 2 cut(s) 101, 266
Kpn2I TCCGGA 1 cut(s) 544
Ksp22I TGATCA 2 cut(s) 522, 759
KspI CCGCGG 1 cut(s) 650
Kzo9I GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
LmnI GCTCC 8 cut(s) 281, 383, 431, 466, 703, 907, 961, 1057
Lsp1109I GCAGC 5 cut(s) 144, 844, 852, 1204, 1231
LweI GCATC 4 cut(s) 672, 1091, 1451, 1513
MaeI CTAG 2 cut(s) 1151, 1487
MaeII ACGT 8 cut(s) 285, 309, 510, 515, 599, 663, 831, 1213
MaeIII GTNAC 3 cut(s) 511, 595, 1463
MalI GATC 8 cut(s) 216, 249, 341, 412, 524, 761, 1359, 1363
MbiI CCGCTC 1 cut(s) 169
MboI GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
MboII GAAGA 8 cut(s) 62, 405, 411, 628, 723, 778, 1240, 1382
MhlI GDGCHC 4 cut(s) 11, 380, 1062, 1727
MlsI TGGCCA 1 cut(s) 980
MluCI AATT 8 cut(s) 32, 124, 481, 563, 1274, 1279, 1400, 1452
MluNI TGGCCA 1 cut(s) 980
Mly113I GGCGCC 2 cut(s) 102, 267
MlyI GAGTC 5 cut(s) 70, 373, 482, 1381, 1471
MmeI TCCRAC 4 cut(s) 177, 306, 405, 453
Mox20I TGGCCA 1 cut(s) 980
Mph1103I ATGCAT 1 cut(s) 1084
MroI TCCGGA 1 cut(s) 544
MscI TGGCCA 1 cut(s) 980
MseI TTAA 1 cut(s) 1496
MslI CAYNNNNRTG 1 cut(s) 830
Msp20I TGGCCA 1 cut(s) 980
MspA1I CMGCKG 1 cut(s) 649
MspI CCGG 8 cut(s) 197, 203, 495, 545, 574, 610, 653, 896
MspR9I CCNGG 6 cut(s) 23, 197, 203, 495, 677, 896
Mva1269I GAATGC 2 cut(s) 849, 1349
MvaI CCWGG 2 cut(s) 23, 677
MvnI CGCG 4 cut(s) 246, 281, 409, 649
MwoI GCNNNNNNNGC 5 cut(s) 166, 273, 871, 1244, 1253
NarI GGCGCC 2 cut(s) 102, 267
NciI CCSGG 4 cut(s) 197, 203, 495, 896
NdeII GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
NlaIII CATG 8 cut(s) 142, 1054, 1094, 1126, 1228, 1316, 1610, 1627
NlaIV GGNNCC 4 cut(s) 103, 201, 268, 903
NmeAIII GCCGAG 3 cut(s) 643, 1687, 1720
NmuCI GTSAC 3 cut(s) 511, 595, 1463
NruI TCGCGA 1 cut(s) 409
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 4 cut(s) 1054, 1094, 1610, 1627
NspV TTCGAA 4 cut(s) 44, 122, 452, 998
OliI CACNNNNGTG 1 cut(s) 830
PaeI GCATGC 2 cut(s) 1094, 1610
PaeR7I CTCGAG 1 cut(s) 1061
PceI AGGCCT 2 cut(s) 623, 1190
PciI ACATGT 1 cut(s) 1623
PcsI WCGNNNNNNNCGW 4 cut(s) 72, 119, 413, 533
PctI GAATGC 2 cut(s) 849, 1349
PfeI GAWTC 4 cut(s) 209, 454, 1097, 1557
Pfl23II CGTACG 1 cut(s) 282
PflMI CCANNNNNTGG 1 cut(s) 987
PfoI TCCNGGA 1 cut(s) 493
Ple19I CGATCG 1 cut(s) 342
PleI GAGTC 5 cut(s) 69, 372, 481, 1381, 1471
PluTI GGCGCC 2 cut(s) 105, 270
PpsI GAGTC 5 cut(s) 69, 372, 481, 1381, 1471
Ppu21I YACGTR 2 cut(s) 516, 1214
PscI ACATGT 1 cut(s) 1623
PsiI TTATAA 1 cut(s) 1160
Psp124BI GAGCTC 3 cut(s) 380, 1062, 1727
Psp6I CCWGG 2 cut(s) 21, 675
PspGI CCWGG 2 cut(s) 21, 675
PspLI CGTACG 1 cut(s) 282
PspN4I GGNNCC 4 cut(s) 103, 201, 268, 903
PspPI GGNCC 5 cut(s) 25, 199, 607, 1045, 1683
PspXI VCTCGAGB 1 cut(s) 1061
PvuI CGATCG 1 cut(s) 342
RruI TCGCGA 1 cut(s) 409
RsaI GTAC 2 cut(s) 284, 731
RsaNI GTAC 2 cut(s) 283, 730
RseI CAYNNNNRTG 1 cut(s) 830
Rsr2I CGGWCCG 1 cut(s) 607
RsrII CGGWCCG 1 cut(s) 607
SacI GAGCTC 3 cut(s) 380, 1062, 1727
SacII CCGCGG 1 cut(s) 650
SalI GTCGAC 2 cut(s) 142, 287
SaqAI TTAA 1 cut(s) 1496
Sau3AI GATC 8 cut(s) 214, 247, 339, 410, 522, 759, 1357, 1361
Sau96I GGNCC 5 cut(s) 25, 199, 607, 1045, 1683
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 5 cut(s) 70, 373, 482, 1381, 1471
ScrFI CCNGG 6 cut(s) 23, 197, 203, 495, 677, 896
SduI GDGCHC 4 cut(s) 11, 380, 1062, 1727
SfaNI GCATC 4 cut(s) 672, 1091, 1451, 1513
SfcI CTRYAG 1 cut(s) 625
SfoI GGCGCC 2 cut(s) 103, 268
Sfr274I CTCGAG 1 cut(s) 1061
Sfr303I CCGCGG 1 cut(s) 650
SfuI TTCGAA 4 cut(s) 44, 122, 452, 998
SgrBI CCGCGG 1 cut(s) 650
SinI GGWCC 4 cut(s) 25, 607, 1045, 1683
SlaI CTCGAG 1 cut(s) 1061
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 3 cut(s) 733, 1061, 1583
SmoI CTYRAG 3 cut(s) 733, 1061, 1583
SphI GCATGC 2 cut(s) 1094, 1610
Sse9I AATT 8 cut(s) 32, 124, 481, 563, 1274, 1279, 1400, 1452
SseBI AGGCCT 2 cut(s) 623, 1190
SspDI GGCGCC 2 cut(s) 101, 266
SspMI CTAG 2 cut(s) 1151, 1487
SstI GAGCTC 3 cut(s) 380, 1062, 1727
StuI AGGCCT 2 cut(s) 623, 1190
StyD4I CCNGG 6 cut(s) 21, 195, 201, 493, 675, 894
StyI CCWWGG 1 cut(s) 1686
TaaI ACNGT 3 cut(s) 448, 1291, 1381
TaiI ACGT 8 cut(s) 288, 312, 513, 518, 602, 666, 834, 1216
TasI AATT 8 cut(s) 32, 124, 481, 563, 1274, 1279, 1400, 1452
TatI WGTACW 1 cut(s) 729
TauI GCSGC 7 cut(s) 85, 102, 580, 649, 652, 749, 1006
TfiI GAWTC 4 cut(s) 209, 454, 1097, 1557
Tru1I TTAA 1 cut(s) 1496
Tru9I TTAA 1 cut(s) 1496
TseFI GTSAC 3 cut(s) 511, 595, 1463
TseI GCWGC 5 cut(s) 132, 857, 865, 1217, 1244
Tsp45I GTSAC 3 cut(s) 511, 595, 1463
TspDTI ATGAA 5 cut(s) 628, 1241, 1398, 1515, 1631
TspGWI ACGGA 3 cut(s) 312, 801, 1327
Van91I CCANNNNNTGG 1 cut(s) 987
VpaK11BI GGWCC 4 cut(s) 25, 607, 1045, 1683
XapI RAATTY 4 cut(s) 481, 563, 1279, 1452
XceI RCATGY 4 cut(s) 1054, 1094, 1610, 1627
XcmI CCANNNNNNNNNTGG 2 cut(s) 35, 1319
XhoI CTCGAG 1 cut(s) 1061
XmiI GTMKAC 2 cut(s) 143, 288
XspI CTAG 2 cut(s) 1151, 1487
ZrmI AGTACT 1 cut(s) 731
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.