MD00G1117700.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000434
Physical Location & Seq
Reverse (-)
25034910 .. 25036109
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1117700.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGCTCGGAAATTGAATATCCTGGCAGCGTTCTTGTTCTTATTGTCAGCATCTAAAACTCAAGCTGAGCCAGGTGTGTTTGATGTCACGAGTGCGACATATGGTGCAAAGGCCGGCTCTGATATCACGCACGCTTTAGCAAAGGCTTGGATTGATGCGTGTGCATCACCGGCACCGAGTAAGGTTGTTGTTCCGAGTGGGACGTACATGTTAACAGAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCTATTGAGATTCAAGTACAAGGGAAATTAACAGCTCCAGCAGACGGCGGCAAACTTACGAGACCGGATACTTGGGTTGGTTTTGACCACGTCAACAAGCTTACCGTATCAGGTGGTGGAACTTTTGACGGCCAAGGAGCACTTGCTTGGAAGAAAAATGACTGCAACAAAAACAAAGATTGCAAATCTATTGCCATCAATATGAGGTTCAACTTCGTTACCAATTCCACAATTCAGGACATAACTTCCCTCGACAGCAAGAATTTCCACGTCAACGTTTTGGGGTGCCACAATGTCACATTCCAACGTGTCAATGTCACGGCACCGGGGGAGAGCATAAATACAGATGGAATCCATATCGGGCGTTCAACTGGAATCAACATTACTGATGCACACATTGGAACTGGGGATGACTGTGTTTCCATTGGCGATGGAAACAAAGAAATCACCGTAACTAGGGTTACTTGTGGACCAGGCCATGGCATAAGCGTTGGAAGTCTTGGAAGGTATCCGAATGAAGAACCTGTGGTTGGAATCAGAGTTAAGAACTGCACCTTGACTAATACATCCAACGGTGTGAGAATCAAGACATGGCCTGCTTCTCCTTCGGATGGTATTGCATCAGATATGCATTTTGAAGATATTATCATGGTCAATGTCAGCAATCCTGTCCTCATAGACCAACAGTACTGCCCGTGGAACCAGTGTAAACAGAACGTTCCATCAAAAGTTAAGATCAGCAATGTCAGCTTTAAAAACATTAAGGGCTCATCTGCAACTCCCCTTGCAGTCCAGATTGTATGCAGCGGTGGCATACCGTGTGAGAATGTGGAATTGGCTGATATTGATCTCACCTACAACGGAAATAAAGGCCCTCTTACCTCTCAATGTTCTAACGTGAAGCCCACCATTACTCGCGTGCCAAATGCTCTTGCGTGTGCTACCTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

400

Amino Acids

42.15

Weight (kDa)

8.32

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 54 - 384 1.8e-104 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 172, 531, 568
AccB7I CCANNNNNTGG 1 cut(s) 725
AccII CGCG 1 cut(s) 1164
AciI CCGC 2 cut(s) 294, 1053
AclI AACGTT 2 cut(s) 522, 963
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 508
AfaI GTAC 3 cut(s) 206, 264, 935
AfiI CCNNNNNNNGG 5 cut(s) 290, 702, 725, 776, 857
AflIII ACRYGT 2 cut(s) 207, 553
AgsI TTSAA 6 cut(s) 16, 229, 260, 457, 615, 884
AjiI CACGTC 2 cut(s) 337, 517
AjnI CCWGG 3 cut(s) 21, 70, 718
AjuI GAANNNNNNNTTGG 4 cut(s) 759, 791, 1064, 1096
AluBI AGCT 4 cut(s) 65, 281, 346, 996
AluI AGCT 4 cut(s) 65, 281, 346, 996
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 1 cut(s) 301
AoxI GGCC 5 cut(s) 111, 376, 721, 839, 1117
ApeKI GCWGC 2 cut(s) 26, 1050
ApoI RAATTY 1 cut(s) 508
AspS9I GGNCC 3 cut(s) 233, 716, 1118
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 3 cut(s) 159, 685, 1090
AvaII GGWCC 2 cut(s) 233, 716
BaeI ACNNNNGTAYC 2 cut(s) 306, 339
BanI GGYRCC 3 cut(s) 172, 531, 568
BanII GRGCYC 1 cut(s) 1016
BauI CACGAG 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 38, 1062
BccI CCATC 5 cut(s) 449, 587, 671, 851, 976
BceAI ACGGC 3 cut(s) 307, 391, 582
BciT130I CCWGG 3 cut(s) 23, 72, 720
BciVI GTATCC 2 cut(s) 307, 765
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 1 cut(s) 702
BfuI GTATCC 2 cut(s) 307, 765
BisI GCNGC 3 cut(s) 27, 295, 1051
BlpI GCTNAGC 1 cut(s) 66
BlsI GCNGC 3 cut(s) 28, 296, 1052
BmcAI AGTACT 1 cut(s) 935
Bme1390I CCNGG 4 cut(s) 23, 72, 573, 720
Bme18I GGWCC 2 cut(s) 233, 716
BmgBI CACGTC 2 cut(s) 337, 517
BmgT120I GGNCC 3 cut(s) 233, 716, 1118
BmiI GGNNCC 5 cut(s) 174, 246, 533, 570, 947
BmrFI CCNGG 4 cut(s) 23, 72, 573, 720
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 5 cut(s) 59, 145, 173, 625, 875
BmuI ACTGGG 1 cut(s) 660
BpmI CTGGAG 1 cut(s) 267
Bpu1102I GCTNAGC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 1092
BsaI GGTCTC 1 cut(s) 301
BsaJI CCNNGG 4 cut(s) 379, 572, 724, 941
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 5 cut(s) 290, 702, 725, 776, 857
Bse118I RCCGGY 2 cut(s) 113, 169
Bse1I ACTGG 3 cut(s) 622, 655, 949
Bse3DI GCAATG 1 cut(s) 994
Bse8I GATNNNNATC 1 cut(s) 1092
BseBI CCWGG 3 cut(s) 23, 72, 720
BseDI CCNNGG 4 cut(s) 379, 572, 724, 941
BseGI GGATG 3 cut(s) 661, 812, 862
BseJI GATNNNNATC 1 cut(s) 1092
BseLI CCNNNNNNNGG 5 cut(s) 290, 702, 725, 776, 857
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 3 cut(s) 622, 655, 949
BseXI GCAGC 2 cut(s) 38, 1062
BsgI GTGCAG 1 cut(s) 781
Bsh1236I CGCG 1 cut(s) 1164
BshFI GGCC 5 cut(s) 113, 378, 723, 841, 1119
BshNI GGYRCC 3 cut(s) 172, 531, 568
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 4 cut(s) 114, 170, 311, 572
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 5 cut(s) 290, 702, 725, 776, 857
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 5 cut(s) 113, 378, 723, 841, 1119
Bso31I GGTCTC 1 cut(s) 301
Bsp1286I GDGCHC 2 cut(s) 388, 1016
Bsp143I GATC 2 cut(s) 981, 1093
Bsp1720I GCTNAGC 1 cut(s) 66
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 2 cut(s) 294, 1053
BspANI GGCC 5 cut(s) 113, 378, 723, 841, 1119
BspCNI CTCAG 1 cut(s) 58
BspFNI CGCG 1 cut(s) 1164
BspLI GGNNCC 5 cut(s) 174, 246, 533, 570, 947
BspT107I GGYRCC 3 cut(s) 172, 531, 568
BspTNI GGTCTC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 994
BsrFI RCCGGY 2 cut(s) 113, 169
BsrI ACTGG 3 cut(s) 622, 655, 949
BssAI RCCGGY 2 cut(s) 113, 169
BssECI CCNNGG 4 cut(s) 379, 572, 724, 941
BssMI GATC 2 cut(s) 981, 1093
BssSI CACGAG 1 cut(s) 88
BssT1I CCWWGG 2 cut(s) 379, 724
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 3 cut(s) 23, 72, 720
Bst4CI ACNGT 6 cut(s) 352, 662, 697, 821, 933, 1065
BstC8I GCNNGC 4 cut(s) 115, 132, 843, 1166
BstDEI CTNAG 1 cut(s) 66
BstDSI CCRYGG 2 cut(s) 724, 941
BstF5I GGATG 3 cut(s) 661, 812, 862
BstFNI CGCG 1 cut(s) 1164
BstKTI GATC 2 cut(s) 984, 1096
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 2 cut(s) 981, 1093
BstMWI GCNNNNNNNGC 3 cut(s) 170, 993, 1056
BstNI CCWGG 3 cut(s) 23, 72, 720
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 4 cut(s) 21, 70, 571, 718
BstUI CGCG 1 cut(s) 1164
BstV1I GCAGC 2 cut(s) 38, 1062
BsuI GTATCC 2 cut(s) 307, 765
BsuRI GGCC 5 cut(s) 113, 378, 723, 841, 1119
BtgI CCRYGG 2 cut(s) 724, 941
BtgZI GCGATG 1 cut(s) 690
BtrI CACGTC 2 cut(s) 337, 517
BtsCI GGATG 3 cut(s) 661, 812, 862
BtsIMutI CAGTG 1 cut(s) 956
Cac8I GCNNGC 4 cut(s) 115, 132, 843, 1166
Cfr10I RCCGGY 2 cut(s) 113, 169
Cfr13I GGNCC 3 cut(s) 233, 716, 1118
Csp6I GTAC 3 cut(s) 205, 263, 934
CviAII CATG 4 cut(s) 208, 725, 837, 895
CviQI GTAC 3 cut(s) 205, 263, 934
DdeI CTNAG 1 cut(s) 66
DpnI GATC 2 cut(s) 983, 1095
DpnII GATC 2 cut(s) 981, 1093
DraI TTTAAA 1 cut(s) 1000
EaeI YGGCCR 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 379, 724
Eco24I GRGCYC 1 cut(s) 1016
Eco31I GGTCTC 1 cut(s) 301
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 233, 716
EcoO109I RGGNCCY 2 cut(s) 233, 1118
EcoRII CCWGG 3 cut(s) 21, 70, 718
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 2 cut(s) 379, 724
EcoT22I ATGCAT 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 2 cut(s) 379, 724
FaeI CATG 4 cut(s) 211, 728, 840, 898
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 1107, 1139
FaqI GGGAC 1 cut(s) 214
FatI CATG 4 cut(s) 207, 724, 836, 894
FauNDI CATATG 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 27, 295, 1051
FokI GGATG 3 cut(s) 668, 799, 869
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 3 cut(s) 27, 295, 1051
FspBI CTAG 1 cut(s) 702
GluI GCNGC 3 cut(s) 27, 295, 1051
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 5 cut(s) 113, 378, 723, 841, 1119
HapII CCGG 4 cut(s) 114, 170, 311, 572
Hin1II CATG 4 cut(s) 211, 728, 840, 898
HincII GTYRAC 3 cut(s) 213, 340, 520
HindII GTYRAC 3 cut(s) 213, 340, 520
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 5 cut(s) 256, 597, 621, 780, 828
HpaI GTTAAC 1 cut(s) 213
HpaII CCGG 4 cut(s) 114, 170, 311, 572
HphI GGTGA 3 cut(s) 159, 685, 1090
Hpy166II GTNNAC 5 cut(s) 213, 340, 520, 716, 956
Hpy188I TCNGA 7 cut(s) 9, 121, 195, 759, 785, 856, 871
Hpy188III TCNNGA 5 cut(s) 88, 482, 832, 1039, 1197
Hpy8I GTNNAC 5 cut(s) 213, 340, 520, 716, 956
HpyAV CCTTC 2 cut(s) 744, 861
HpyCH4III ACNGT 6 cut(s) 352, 662, 697, 821, 933, 1065
HpyCH4IV ACGT 7 cut(s) 203, 336, 516, 522, 553, 963, 1143
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 993, 1056
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 7 cut(s) 203, 336, 516, 522, 553, 963, 1143
Hsp92II CATG 4 cut(s) 211, 728, 840, 898
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
KspAI GTTAAC 1 cut(s) 213
Kzo9I GATC 2 cut(s) 981, 1093
LmnI GCTCC 3 cut(s) 250, 286, 383
Lsp1109I GCAGC 2 cut(s) 38, 1062
LweI GCATC 5 cut(s) 59, 145, 173, 625, 875
MaeI CTAG 1 cut(s) 702
MaeII ACGT 7 cut(s) 203, 336, 516, 522, 553, 963, 1143
MaeIII GTNAC 6 cut(s) 85, 463, 541, 562, 697, 706
MalI GATC 2 cut(s) 983, 1095
MboI GATC 2 cut(s) 981, 1093
MboII GAAGA 4 cut(s) 409, 776, 896, 1185
MhlI GDGCHC 2 cut(s) 388, 1016
MluCI AATT 6 cut(s) 11, 272, 469, 477, 508, 1079
MmeI TCCRAC 4 cut(s) 574, 718, 757, 840
MnlI CCTC 5 cut(s) 444, 506, 929, 1131, 1138
Mph1103I ATGCAT 1 cut(s) 879
MroNI GCCGGC 1 cut(s) 113
MseI TTAA 6 cut(s) 212, 275, 789, 978, 999, 1008
MslI CAYNNNNRTG 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 1053
MspI CCGG 4 cut(s) 114, 170, 311, 572
MspR9I CCNGG 4 cut(s) 23, 72, 573, 720
MvaI CCWGG 3 cut(s) 23, 72, 720
MvnI CGCG 1 cut(s) 1164
MwoI GCNNNNNNNGC 3 cut(s) 170, 993, 1056
NaeI GCCGGC 1 cut(s) 115
NciI CCSGG 1 cut(s) 573
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 100
NdeII GATC 2 cut(s) 981, 1093
NgoMIV GCCGGC 1 cut(s) 113
NlaIII CATG 4 cut(s) 211, 728, 840, 898
NlaIV GGNNCC 5 cut(s) 174, 246, 533, 570, 947
NmuCI GTSAC 3 cut(s) 85, 541, 562
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 1 cut(s) 211
PciI ACATGT 1 cut(s) 207
PdiI GCCGGC 1 cut(s) 115
PfeI GAWTC 5 cut(s) 256, 597, 621, 780, 828
PflFI GACNNNGTC 1 cut(s) 335
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 3 cut(s) 28, 296, 1052
PpuMI RGGWCCY 1 cut(s) 233
PscI ACATGT 1 cut(s) 207
Psp1406I AACGTT 2 cut(s) 522, 963
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 3 cut(s) 21, 70, 718
PspGI CCWGG 3 cut(s) 21, 70, 718
PspN4I GGNNCC 5 cut(s) 174, 246, 533, 570, 947
PspPI GGNCC 3 cut(s) 233, 716, 1118
PspPPI RGGWCCY 1 cut(s) 233
PsyI GACNNNGTC 1 cut(s) 335
RsaI GTAC 3 cut(s) 206, 264, 935
RsaNI GTAC 3 cut(s) 205, 263, 934
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 6 cut(s) 212, 275, 789, 978, 999, 1008
SatI GCNGC 3 cut(s) 27, 295, 1051
Sau3AI GATC 2 cut(s) 981, 1093
Sau96I GGNCC 3 cut(s) 233, 716, 1118
ScaI AGTACT 1 cut(s) 935
ScrFI CCNGG 4 cut(s) 23, 72, 573, 720
SduI GDGCHC 2 cut(s) 388, 1016
SfaNI GCATC 5 cut(s) 59, 145, 173, 625, 875
SinI GGWCC 2 cut(s) 233, 716
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
Sse9I AATT 6 cut(s) 11, 272, 469, 477, 508, 1079
SsiI CCGC 2 cut(s) 294, 1053
SspMI CTAG 1 cut(s) 702
StyD4I CCNGG 4 cut(s) 21, 70, 571, 718
StyI CCWWGG 2 cut(s) 379, 724
TaaI ACNGT 6 cut(s) 352, 662, 697, 821, 933, 1065
TaiI ACGT 7 cut(s) 206, 339, 519, 525, 556, 966, 1146
TaqI TCGA 1 cut(s) 498
TasI AATT 6 cut(s) 11, 272, 469, 477, 508, 1079
TatI WGTACW 2 cut(s) 262, 933
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 5 cut(s) 256, 597, 621, 780, 828
Tru1I TTAA 6 cut(s) 212, 275, 789, 978, 999, 1008
Tru9I TTAA 6 cut(s) 212, 275, 789, 978, 999, 1008
TscAI CASTG 1 cut(s) 956
TseFI GTSAC 3 cut(s) 85, 541, 562
TseI GCWGC 2 cut(s) 26, 1050
Tsp45I GTSAC 3 cut(s) 85, 541, 562
TspDTI ATGAA 1 cut(s) 777
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 1 cut(s) 956
Tth111I GACNNNGTC 1 cut(s) 335
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 2 cut(s) 233, 716
XapI RAATTY 1 cut(s) 508
XceI RCATGY 1 cut(s) 211
XspI CTAG 1 cut(s) 702
ZrmI AGTACT 1 cut(s) 935
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.