RLG00000030246

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000434
Physical Location & Seq
Forward (+)
58301243 .. 58303907
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030246

Sequence Viewer

Length: 1146 bp
ATGCTGGGAATGTCGCCGGGTACCCGCAAGCTTCATGACAGACTTGAGCATGAAGGTAGATCTAGGGATCTTTTAGCCGGTGTTGGCTTTGACGTGACCACCGCAAAATATGGTGCAAAGCCCAATTCTGATATTACTCAGGCTTTAGCAAATGCATGGAAAGATGCATGTGCATCTACCTCGGCAAGTAAAGTTATTGTTCCCAAAGGGACATACAAACTGAAAGAAGCAAGCTTCAAAGGTCCTTGCAAGGCTCCTATTGAGGTTCAGGTCCAAGGTACTTTGCATGCGCCACCAGACGGCACCCAACTATCGAAACCAGATACGTGGATCGAGTTTTCATACCTTGACAGGTTCACATTATCGGGTAGTAGAACCTTTGATGGCCAAGGACAAAAAGCTTGGAAAGAAAATGATTGCCACAAAAACCCAAAGTTCCACTCTGTCAAGAACTCATTAGTCAAGGATGTAACTTCACGTAACAGCAAGAATTTCCACGTGAATGTATTATCGTGCGAGCAACTTACATTCCAACATTTCACCGTCGCTGCACCAGGAGATAGCGTTAATACAGATGGAATACATATTGGGCGCTCAATCGGGGTTAATGTTACTGATTCAAAGATTGGTACAGGAGACGATTGCATTTCAATAGGTGATGGCACCAAGCAACTAACTATTACTAAGGTAACTTGTGGACCAGGCCATGGTATAAGCATAGGAAGTCTTGGAAGGTACCCAAAAGAAGAACCTGTGAGTGGAATCCATATTAGGAACTGTACCCTCAGCAATACGTCCAACGGTGTTAGAATCAAGACATGGCCTAATTCTCCTGCAGCTAGCACTGCCTCAGATATTCACTTTGAGGATATCATCATGAATAATGTTGGTAACCCAATCCTCATTGATCAAGAGTACTGCCCATGGAATCAGTGTAATAAACAGGCTTCATCAAAAATCAAGATCAGCAATGTGAGCTTCAAAAACATTAGAGGTACGTCTTCCACTCCGGTGGCCGTGAAGATTGCATGCGCCAAGGGCTTACCATGTGACAAGGTGGAGATGACTGACATTAATCTCAAGTACAGTGGAAAGCAAGGTTCTATTACATCTCTGGCACGTTTACTTACTTGGAATGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

382

Amino Acids

41.22

Weight (kDa)

9.07

Isoelectric Point (pI)

28.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 57 - 369 1.5e-101 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 20, 735
AccB1I GGYRCC 4 cut(s) 20, 302, 662, 735
AccB7I CCANNNNNTGG 1 cut(s) 707
AciI CCGC 2 cut(s) 25, 102
AclWI GGATC 2 cut(s) 75, 338
AcoI YGGCCR 2 cut(s) 385, 1014
AcsI RAATTY 1 cut(s) 490
AcvI CACGTG 1 cut(s) 499
AfaI GTAC 8 cut(s) 22, 280, 631, 737, 781, 917, 997, 1085
AfiI CCNNNNNNNGG 3 cut(s) 299, 707, 758
AgsI TTSAA 4 cut(s) 238, 621, 651, 982
AjiI CACGTC 1 cut(s) 94
AjnI CCWGG 2 cut(s) 553, 700
AleI CACNNNNGTG 1 cut(s) 1010
AluBI AGCT 5 cut(s) 31, 234, 401, 839, 978
AluI AGCT 5 cut(s) 31, 234, 401, 839, 978
Alw26I GTCTC 1 cut(s) 630
AlwI GGATC 2 cut(s) 75, 338
AoxI GGCC 4 cut(s) 385, 703, 821, 1014
ApeKI GCWGC 2 cut(s) 548, 836
ApoI RAATTY 1 cut(s) 490
AseI ATTAAT 1 cut(s) 1074
Asp718I GGTACC 2 cut(s) 20, 735
AspLEI GCGC 3 cut(s) 292, 594, 1034
AspS9I GGNCC 3 cut(s) 242, 271, 698
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 2 cut(s) 532, 668
AsuNHI GCTAGC 1 cut(s) 839
AvaII GGWCC 3 cut(s) 242, 271, 698
BalI TGGCCA 1 cut(s) 387
BanI GGYRCC 4 cut(s) 20, 302, 662, 735
BbrPI CACGTG 1 cut(s) 499
BbsI GAAGAC 1 cut(s) 993
BbvCI CCTCAGC 1 cut(s) 785
BbvI GCAGC 2 cut(s) 535, 848
BccI CCATC 3 cut(s) 377, 569, 653
BceAI ACGGC 2 cut(s) 316, 1001
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 2 cut(s) 555, 702
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 1 cut(s) 630
BfaI CTAG 2 cut(s) 63, 840
BfmI CTRYAG 1 cut(s) 834
BfoI RGCGCY 1 cut(s) 595
BglII AGATCT 1 cut(s) 59
BisI GCNGC 2 cut(s) 549, 837
BlsI GCNGC 2 cut(s) 550, 838
BmcAI AGTACT 1 cut(s) 917
Bme1390I CCNGG 3 cut(s) 18, 555, 702
Bme18I GGWCC 3 cut(s) 242, 271, 698
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 3 cut(s) 242, 271, 698
BmiI GGNNCC 5 cut(s) 22, 255, 304, 664, 737
BmrFI CCNGG 3 cut(s) 18, 555, 702
BmsI GCATC 2 cut(s) 154, 182
BmtI GCTAGC 1 cut(s) 843
BpiI GAAGAC 1 cut(s) 993
Bpu10I CCTNAGC 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 65, 1064
BpuMI CCSGG 1 cut(s) 18
BsaAI YACGTR 3 cut(s) 327, 479, 499
BsaJI CCNNGG 6 cut(s) 180, 274, 388, 706, 923, 1035
BsaWI WCCGGW 1 cut(s) 1009
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 299, 707, 758
Bse118I RCCGGY 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 976
BseBI CCWGG 2 cut(s) 555, 702
BseDI CCNNGG 6 cut(s) 180, 274, 388, 706, 923, 1035
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 3 cut(s) 299, 707, 758
BseMI GCAATG 1 cut(s) 976
BseMII CTCAG 3 cut(s) 152, 799, 864
BseXI GCAGC 2 cut(s) 535, 848
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 1 cut(s) 534
BshFI GGCC 4 cut(s) 387, 705, 823, 1016
BshNI GGYRCC 4 cut(s) 20, 302, 662, 735
BsiSI CCGG 3 cut(s) 17, 78, 1010
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 3 cut(s) 299, 707, 758
BsmAI GTCTC 1 cut(s) 630
BsmBI CGTCTC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 4 cut(s) 387, 705, 823, 1016
Bsp143I GATC 5 cut(s) 59, 67, 330, 907, 963
Bsp19I CCATGG 2 cut(s) 706, 923
BspACI CCGC 2 cut(s) 25, 102
BspANI GGCC 4 cut(s) 387, 705, 823, 1016
BspCNI CTCAG 3 cut(s) 151, 798, 863
BspHI TCATGA 2 cut(s) 34, 876
BspLI GGNNCC 5 cut(s) 22, 255, 304, 664, 737
BspMAI CTGCAG 1 cut(s) 838
BspOI GCTAGC 1 cut(s) 843
BspPI GGATC 2 cut(s) 75, 338
BspT107I GGYRCC 4 cut(s) 20, 302, 662, 735
BsrDI GCAATG 1 cut(s) 976
BsrFI RCCGGY 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 6 cut(s) 180, 274, 388, 706, 923, 1035
BssMI GATC 5 cut(s) 59, 67, 330, 907, 963
BssT1I CCWWGG 5 cut(s) 274, 388, 706, 923, 1035
Bst2UI CCWGG 2 cut(s) 555, 702
Bst4CI ACNGT 4 cut(s) 544, 779, 803, 1088
BstBAI YACGTR 3 cut(s) 327, 479, 499
BstC8I GCNNGC 6 cut(s) 29, 232, 288, 518, 841, 1030
BstDEI CTNAG 4 cut(s) 138, 684, 785, 850
BstDSI CCRYGG 2 cut(s) 706, 923
BstEII GGTNACC 1 cut(s) 890
BstF5I GGATG 1 cut(s) 472
BstH2I RGCGCY 1 cut(s) 595
BstHHI GCGC 3 cut(s) 292, 594, 1034
BstKTI GATC 5 cut(s) 62, 70, 333, 910, 966
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 5 cut(s) 59, 67, 330, 907, 963
BstMWI GCNNNNNNNGC 3 cut(s) 845, 975, 1038
BstNI CCWGG 2 cut(s) 555, 702
BstNSI RCATGY 3 cut(s) 171, 290, 1032
BstPI GGTNACC 1 cut(s) 890
BstSCI CCNGG 3 cut(s) 16, 553, 700
BstSFI CTRYAG 1 cut(s) 834
BstV1I GCAGC 2 cut(s) 535, 848
BstV2I GAAGAC 1 cut(s) 993
BstX2I RGATCY 2 cut(s) 59, 67
BstXI CCANNNNNNTGG 2 cut(s) 327, 1012
BstYI RGATCY 2 cut(s) 59, 67
BsuRI GGCC 4 cut(s) 387, 705, 823, 1016
BtgI CCRYGG 2 cut(s) 706, 923
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 1 cut(s) 472
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 3 cut(s) 843, 938, 1093
Cac8I GCNNGC 6 cut(s) 29, 232, 288, 518, 841, 1030
CciI TCATGA 2 cut(s) 34, 876
CfoI GCGC 3 cut(s) 292, 594, 1034
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 3 cut(s) 242, 271, 698
Csp6I GTAC 8 cut(s) 21, 279, 630, 736, 780, 916, 996, 1084
CspCI CAANNNNNGTGG 2 cut(s) 1069, 1104
CviQI GTAC 8 cut(s) 21, 279, 630, 736, 780, 916, 996, 1084
DdeI CTNAG 4 cut(s) 138, 684, 785, 850
DpnI GATC 5 cut(s) 61, 69, 332, 909, 965
DpnII GATC 5 cut(s) 59, 67, 330, 907, 963
EaeI YGGCCR 2 cut(s) 385, 1014
Eco130I CCWWGG 5 cut(s) 274, 388, 706, 923, 1035
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 3 cut(s) 242, 271, 698
Eco72I CACGTG 1 cut(s) 499
Eco91I GGTNACC 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 242
EcoO65I GGTNACC 1 cut(s) 890
EcoRII CCWGG 2 cut(s) 553, 700
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 5 cut(s) 274, 388, 706, 923, 1035
EcoT22I ATGCAT 2 cut(s) 157, 169
ErhI CCWWGG 5 cut(s) 274, 388, 706, 923, 1035
Esp3I CGTCTC 1 cut(s) 630
FaqI GGGAC 1 cut(s) 223
FauI CCCGC 1 cut(s) 32
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 2 cut(s) 549, 837
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 2 cut(s) 549, 837
FspBI CTAG 2 cut(s) 63, 840
GlaI GCGC 3 cut(s) 291, 593, 1033
GluI GCNGC 2 cut(s) 549, 837
GsaI CCCAGC 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 595
HaeIII GGCC 4 cut(s) 387, 705, 823, 1016
HapII CCGG 3 cut(s) 17, 78, 1010
HhaI GCGC 3 cut(s) 292, 594, 1034
Hin6I GCGC 3 cut(s) 290, 592, 1032
HinP1I GCGC 3 cut(s) 290, 592, 1032
HindIII AAGCTT 3 cut(s) 29, 232, 399
HinfI GANTC 4 cut(s) 617, 762, 810, 928
HpaII CCGG 3 cut(s) 17, 78, 1010
HphI GGTGA 2 cut(s) 532, 668
Hpy166II GTNNAC 3 cut(s) 357, 698, 1124
Hpy188I TCNGA 2 cut(s) 130, 853
Hpy188III TCNNGA 6 cut(s) 35, 448, 814, 877, 911, 961
Hpy8I GTNNAC 3 cut(s) 357, 698, 1124
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 2 cut(s) 47, 726
HpyCH4III ACNGT 4 cut(s) 544, 779, 803, 1088
HpyCH4IV ACGT 7 cut(s) 93, 326, 478, 498, 794, 998, 1120
HpyF10VI GCNNNNNNNGC 3 cut(s) 845, 975, 1038
HpyF3I CTNAG 4 cut(s) 138, 684, 785, 850
HpySE526I ACGT 7 cut(s) 93, 326, 478, 498, 794, 998, 1120
HspAI GCGC 3 cut(s) 290, 592, 1032
KpnI GGTACC 2 cut(s) 24, 739
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 5 cut(s) 59, 67, 330, 907, 963
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 2 cut(s) 535, 848
LweI GCATC 2 cut(s) 154, 182
MaeI CTAG 2 cut(s) 63, 840
MaeII ACGT 7 cut(s) 93, 326, 478, 498, 794, 998, 1120
MaeIII GTNAC 7 cut(s) 94, 469, 479, 610, 688, 890, 1049
MalI GATC 5 cut(s) 61, 69, 332, 909, 965
MboI GATC 5 cut(s) 59, 67, 330, 907, 963
MboII GAAGA 3 cut(s) 758, 993, 1033
MflI RGATCY 2 cut(s) 59, 67
MlsI TGGCCA 1 cut(s) 387
MluCI AATT 3 cut(s) 124, 490, 826
MluNI TGGCCA 1 cut(s) 387
MmeI TCCRAC 2 cut(s) 556, 822
MnlI CCTC 7 cut(s) 190, 256, 794, 859, 859, 911, 986
Mox20I TGGCCA 1 cut(s) 387
Mph1103I ATGCAT 2 cut(s) 157, 169
MscI TGGCCA 1 cut(s) 387
MseI TTAA 3 cut(s) 567, 606, 1074
MslI CAYNNNNRTG 2 cut(s) 501, 1010
Msp20I TGGCCA 1 cut(s) 387
MspI CCGG 3 cut(s) 17, 78, 1010
MspR9I CCNGG 3 cut(s) 18, 555, 702
MvaI CCWGG 2 cut(s) 555, 702
MwoI GCNNNNNNNGC 3 cut(s) 845, 975, 1038
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 2 cut(s) 706, 923
NdeII GATC 5 cut(s) 59, 67, 330, 907, 963
NheI GCTAGC 1 cut(s) 839
NlaIV GGNNCC 5 cut(s) 22, 255, 304, 664, 737
NmeAIII GCCGAG 1 cut(s) 161
NmuCI GTSAC 2 cut(s) 94, 1049
NsiI ATGCAT 2 cut(s) 157, 169
NspI RCATGY 3 cut(s) 171, 290, 1032
OliI CACNNNNGTG 1 cut(s) 1010
PaeI GCATGC 2 cut(s) 290, 1032
PagI TCATGA 2 cut(s) 34, 876
PfeI GAWTC 4 cut(s) 617, 762, 810, 928
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 2 cut(s) 550, 838
PmaCI CACGTG 1 cut(s) 499
PmlI CACGTG 1 cut(s) 499
Ppu21I YACGTR 3 cut(s) 327, 479, 499
PpuMI RGGWCCY 1 cut(s) 242
PshBI ATTAAT 1 cut(s) 1074
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 2 cut(s) 553, 700
PspCI CACGTG 1 cut(s) 499
PspEI GGTNACC 1 cut(s) 890
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 553, 700
PspN4I GGNNCC 5 cut(s) 22, 255, 304, 664, 737
PspPI GGNCC 3 cut(s) 242, 271, 698
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 838
PsuI RGATCY 2 cut(s) 59, 67
RsaI GTAC 8 cut(s) 22, 280, 631, 737, 781, 917, 997, 1085
RsaNI GTAC 8 cut(s) 21, 279, 630, 736, 780, 916, 996, 1084
RseI CAYNNNNRTG 2 cut(s) 501, 1010
SaqAI TTAA 3 cut(s) 567, 606, 1074
SatI GCNGC 2 cut(s) 549, 837
Sau3AI GATC 5 cut(s) 59, 67, 330, 907, 963
Sau96I GGNCC 3 cut(s) 242, 271, 698
ScaI AGTACT 1 cut(s) 917
ScrFI CCNGG 3 cut(s) 18, 555, 702
SfaNI GCATC 2 cut(s) 154, 182
SfcI CTRYAG 1 cut(s) 834
SinI GGWCC 3 cut(s) 242, 271, 698
SmiMI CAYNNNNRTG 2 cut(s) 501, 1010
SmlI CTYRAG 2 cut(s) 44, 1079
SmoI CTYRAG 2 cut(s) 44, 1079
SphI GCATGC 2 cut(s) 290, 1032
Sse9I AATT 3 cut(s) 124, 490, 826
SsiI CCGC 2 cut(s) 25, 102
SspMI CTAG 2 cut(s) 63, 840
StyD4I CCNGG 3 cut(s) 16, 553, 700
StyI CCWWGG 5 cut(s) 274, 388, 706, 923, 1035
TaaI ACNGT 4 cut(s) 544, 779, 803, 1088
TaiI ACGT 7 cut(s) 96, 329, 481, 501, 797, 1001, 1123
TaqI TCGA 2 cut(s) 314, 333
TasI AATT 3 cut(s) 124, 490, 826
TatI WGTACW 2 cut(s) 915, 1083
TfiI GAWTC 4 cut(s) 617, 762, 810, 928
Tru1I TTAA 3 cut(s) 567, 606, 1074
Tru9I TTAA 3 cut(s) 567, 606, 1074
TscAI CASTG 3 cut(s) 850, 938, 1093
TseFI GTSAC 2 cut(s) 94, 1049
TseI GCWGC 2 cut(s) 548, 836
Tsp45I GTSAC 2 cut(s) 94, 1049
TspDTI ATGAA 5 cut(s) 23, 66, 330, 893, 939
TspRI CASTG 3 cut(s) 850, 938, 1093
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 3 cut(s) 242, 271, 698
VspI ATTAAT 1 cut(s) 1074
XapI RAATTY 1 cut(s) 490
XceI RCATGY 3 cut(s) 171, 290, 1032
XspI CTAG 2 cut(s) 63, 840
ZrmI AGTACT 1 cut(s) 917
Zsp2I ATGCAT 2 cut(s) 157, 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.