RLG00000021134

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000434
Physical Location & Seq
Forward (+)
72196298 .. 72197684
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021134

Sequence Viewer

Length: 960 bp
ATGGGTCTCAAATTGATGACTATCATGGCGATATTCATTTTCTTATTGTTAGCAGCTGCAGCTAAAGCCCAACCCCGTGTAGTCTTTGACGTGACGAGTGCAAAATATGGTGGAAAAACTAACTATGATATTACAGACTCCTTAGCACAAGCTTGGCCAGATGCTTGTGCTTCTGTATGGCCGAGTAAACTCGTTGTTCCAAGCGGCACATATTGGTTGAGAGGAGCAACTTTTATAGGTCCTTGCAAGGCTCCTATCGAGGTGCAAATCCAAGGAAAGTTGAAAGCTCCCGAAGATGGCCACCAACTCGTGAACTCGGATTTTTGGGTTGCTTTTCGCATAATTTCCCTTGATTGGAATACTTATCGTGCTATTAACGTGAGATTCGACTACATCTCCAACTCCATAATTCAGGACATAATTTCCCTTAACAGCAGAAGTTTCCACATTAACGTTTACGCGTGCCATGATGTTACATTCCAGCGTGTGACTGTCATAGCACCCAGCGAGAGTTCTAATACAGATGGAATCCACATTGCACCTTCATGGAGGATCAACGTAACGGTTACAAACATCGGAACCGGAGATGATTGTATTTCCATCGGTGATGGCACTTCGCAACTCATAGTAACCAATGTTAATTGTGGACCGGGACATGGAATAAGCGTAGGAAGTCTTGGAAAGTATCTTGATGAAAAACTGGCTCAGTCGAACGTTAAGATTAGTAATGTCAGCTTCAAGAACATCAGAGGCTCATCTGCATCACAAGTTGCCGTCAATCTTGCATGTAGTGGGAGCTTACCATGTGAGAATGTAGAACTGATAGGCATTGATCTCGTATACAGTGGGGATGGCGATCGACCTATTACATCTCGATGCTCTAATGTCGTCAAGCCCACAATTATTAACGTGAGTGAGGCTCTTGCTTGCAGACCCTACCCAATAAATCAGAATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

320

Amino Acids

34.46

Weight (kDa)

6.51

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 127 - 232 2.4e-33 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 232 - 298 1.6e-09 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 840
AccII CGCG 1 cut(s) 461
AciI CCGC 1 cut(s) 204
AclI AACGTT 2 cut(s) 453, 714
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 3 cut(s) 155, 179, 298
AfiI CCNNNNNNNGG 3 cut(s) 296, 354, 656
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 2 cut(s) 283, 739
AjiI CACGTC 1 cut(s) 91
AluBI AGCT 6 cut(s) 56, 62, 152, 287, 735, 798
AluI AGCT 6 cut(s) 56, 62, 152, 287, 735, 798
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 1 cut(s) 560
AoxI GGCC 3 cut(s) 155, 179, 298
ApeKI GCWGC 3 cut(s) 53, 56, 59
AspS9I GGNCC 2 cut(s) 239, 647
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 1 cut(s) 617
AvaII GGWCC 2 cut(s) 239, 647
BalI TGGCCA 2 cut(s) 157, 300
BauI CACGAG 1 cut(s) 308
BbvI GCAGC 3 cut(s) 43, 65, 71
BccI CCATC 5 cut(s) 290, 518, 602, 608, 845
BceAI ACGGC 1 cut(s) 758
BcgI CGANNNNNNTGC 2 cut(s) 817, 851
BcnI CCSGG 1 cut(s) 651
BcoDI GTCTC 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 4 cut(s) 54, 57, 60, 205
BlsI GCNGC 4 cut(s) 55, 58, 61, 206
Bme1390I CCNGG 1 cut(s) 651
Bme18I GGWCC 2 cut(s) 239, 647
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 239, 647
BmiI GGNNCC 2 cut(s) 252, 580
BmrFI CCNGG 1 cut(s) 651
BmsI GCATC 3 cut(s) 151, 770, 866
BplI GAGNNNNNCTC 2 cut(s) 904, 936
Bpu10I CCTNAGC 1 cut(s) 142
BpuMI CCSGG 1 cut(s) 651
BsaBI GATNNNNATC 2 cut(s) 20, 855
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 271
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 354, 656
Bse1I ACTGG 1 cut(s) 705
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 2 cut(s) 20, 855
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 1 cut(s) 856
BseJI GATNNNNATC 2 cut(s) 20, 855
BseLI CCNNNNNNNGG 3 cut(s) 296, 354, 656
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 1 cut(s) 719
BseNI ACTGG 1 cut(s) 705
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 3 cut(s) 43, 65, 71
BseYI CCCAGC 1 cut(s) 503
Bsh1236I CGCG 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 859
BshFI GGCC 3 cut(s) 157, 181, 300
BsiEI CGRYCG 1 cut(s) 859
BsiSI CCGG 2 cut(s) 582, 650
BslFI GGGAC 1 cut(s) 666
BslI CCNNNNNNNGG 3 cut(s) 296, 354, 656
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 666
BsnI GGCC 3 cut(s) 157, 181, 300
Bso31I GGTCTC 1 cut(s) 11
Bsp143I GATC 3 cut(s) 552, 832, 856
BspACI CCGC 1 cut(s) 204
BspANI GGCC 3 cut(s) 157, 181, 300
BspCNI CTCAG 1 cut(s) 718
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 2 cut(s) 252, 580
BspMAI CTGCAG 1 cut(s) 61
BspPI GGATC 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 1 cut(s) 705
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 3 cut(s) 552, 832, 856
BssNAI GTATAC 1 cut(s) 841
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 271
Bst1107I GTATAC 1 cut(s) 841
Bst2BI CACGAG 1 cut(s) 308
Bst4CI ACNGT 3 cut(s) 493, 565, 845
BstC8I GCNNGC 2 cut(s) 463, 928
BstDEI CTNAG 2 cut(s) 142, 705
BstF5I GGATG 1 cut(s) 856
BstFNI CGCG 1 cut(s) 461
BstKTI GATC 3 cut(s) 555, 835, 859
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 3 cut(s) 552, 832, 856
BstMCI CGRYCG 1 cut(s) 859
BstMWI GCNNNNNNNGC 2 cut(s) 59, 65
BstNSI RCATGY 1 cut(s) 789
BstSCI CCNGG 1 cut(s) 649
BstSFI CTRYAG 1 cut(s) 57
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 3 cut(s) 43, 65, 71
BstZ17I GTATAC 1 cut(s) 841
BsuRI GGCC 3 cut(s) 157, 181, 300
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 1 cut(s) 856
BtsIMutI CAGTG 1 cut(s) 850
Cac8I GCNNGC 2 cut(s) 463, 928
Cfr13I GGNCC 2 cut(s) 239, 647
CviAII CATG 6 cut(s) 25, 467, 546, 656, 786, 804
DdeI CTNAG 2 cut(s) 142, 705
DpnI GATC 3 cut(s) 554, 834, 858
DpnII GATC 3 cut(s) 552, 832, 856
EaeI YGGCCR 3 cut(s) 155, 179, 298
Eco130I CCWWGG 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 239, 647
EcoO109I RGGNCCY 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 6 cut(s) 28, 470, 549, 659, 789, 807
FaqI GGGAC 1 cut(s) 666
FatI CATG 6 cut(s) 24, 466, 545, 655, 785, 803
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 4 cut(s) 54, 57, 60, 205
FokI GGATG 1 cut(s) 863
Fsp4HI GCNGC 4 cut(s) 54, 57, 60, 205
GluI GCNGC 4 cut(s) 54, 57, 60, 205
GsaI CCCAGC 1 cut(s) 507
HaeIII GGCC 3 cut(s) 157, 181, 300
HapII CCGG 2 cut(s) 582, 650
Hin1II CATG 6 cut(s) 28, 470, 549, 659, 789, 807
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 3 cut(s) 137, 384, 528
HpaII CCGG 2 cut(s) 582, 650
HphI GGTGA 1 cut(s) 617
Hpy166II GTNNAC 5 cut(s) 188, 313, 457, 647, 841
Hpy188I TCNGA 4 cut(s) 319, 578, 749, 951
Hpy188III TCNNGA 6 cut(s) 290, 310, 413, 689, 739, 873
Hpy8I GTNNAC 5 cut(s) 188, 313, 457, 647, 841
HpyAV CCTTC 1 cut(s) 552
HpyCH4III ACNGT 3 cut(s) 493, 565, 845
HpyCH4IV ACGT 6 cut(s) 90, 378, 453, 558, 714, 909
HpyCH4V TGCA 8 cut(s) 59, 101, 246, 265, 539, 761, 785, 930
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 65
HpyF3I CTNAG 2 cut(s) 142, 705
HpySE526I ACGT 6 cut(s) 90, 378, 453, 558, 714, 909
Hsp92II CATG 6 cut(s) 28, 470, 549, 659, 789, 807
Kzo9I GATC 3 cut(s) 552, 832, 856
LmnI GCTCC 4 cut(s) 224, 256, 292, 795
LpnPI CCDG 7 cut(s) 171, 398, 494, 517, 595, 663, 686
Lsp1109I GCAGC 3 cut(s) 43, 65, 71
LweI GCATC 3 cut(s) 151, 770, 866
MaeII ACGT 6 cut(s) 90, 378, 453, 558, 714, 909
MaeIII GTNAC 6 cut(s) 91, 472, 487, 559, 565, 628
MalI GATC 3 cut(s) 554, 834, 858
MboI GATC 3 cut(s) 552, 832, 856
MboII GAAGA 1 cut(s) 305
MlsI TGGCCA 2 cut(s) 157, 300
MluCI AATT 6 cut(s) 11, 342, 408, 420, 640, 900
MluI ACGCGT 1 cut(s) 459
MluNI TGGCCA 2 cut(s) 157, 300
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 5 cut(s) 215, 253, 543, 743, 910
Mox20I TGGCCA 2 cut(s) 157, 300
MscI TGGCCA 2 cut(s) 157, 300
MseI TTAA 6 cut(s) 375, 429, 450, 639, 717, 906
MslI CAYNNNNRTG 2 cut(s) 544, 874
Msp20I TGGCCA 2 cut(s) 157, 300
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 2 cut(s) 582, 650
MspR9I CCNGG 1 cut(s) 651
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 59, 65
NciI CCSGG 1 cut(s) 651
NdeII GATC 3 cut(s) 552, 832, 856
NlaIII CATG 6 cut(s) 28, 470, 549, 659, 789, 807
NlaIV GGNNCC 2 cut(s) 252, 580
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 2 cut(s) 91, 487
NspI RCATGY 1 cut(s) 789
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PfeI GAWTC 2 cut(s) 384, 528
PkrI GCNGC 4 cut(s) 55, 58, 61, 206
Ple19I CGATCG 1 cut(s) 859
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PpuMI RGGWCCY 1 cut(s) 239
Psp1406I AACGTT 2 cut(s) 453, 714
Psp5II RGGWCCY 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 503
PspN4I GGNNCC 2 cut(s) 252, 580
PspPI GGNCC 2 cut(s) 239, 647
PspPPI RGGWCCY 1 cut(s) 239
PstI CTGCAG 1 cut(s) 61
PvuI CGATCG 1 cut(s) 859
PvuII CAGCTG 1 cut(s) 56
RseI CAYNNNNRTG 2 cut(s) 544, 874
SaqAI TTAA 6 cut(s) 375, 429, 450, 639, 717, 906
SatI GCNGC 4 cut(s) 54, 57, 60, 205
Sau3AI GATC 3 cut(s) 552, 832, 856
Sau96I GGNCC 2 cut(s) 239, 647
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 651
SfaNI GCATC 3 cut(s) 151, 770, 866
SfcI CTRYAG 1 cut(s) 57
SinI GGWCC 2 cut(s) 239, 647
SmiMI CAYNNNNRTG 2 cut(s) 544, 874
Sse9I AATT 6 cut(s) 11, 342, 408, 420, 640, 900
SsiI CCGC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 649
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 3 cut(s) 493, 565, 845
TaiI ACGT 6 cut(s) 93, 381, 456, 561, 717, 912
TaqI TCGA 5 cut(s) 258, 387, 710, 859, 874
TasI AATT 6 cut(s) 11, 342, 408, 420, 640, 900
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 2 cut(s) 384, 528
Tru1I TTAA 6 cut(s) 375, 429, 450, 639, 717, 906
Tru9I TTAA 6 cut(s) 375, 429, 450, 639, 717, 906
TscAI CASTG 1 cut(s) 850
TseFI GTSAC 2 cut(s) 91, 487
TseI GCWGC 3 cut(s) 53, 56, 59
Tsp45I GTSAC 2 cut(s) 91, 487
TspDTI ATGAA 3 cut(s) 25, 534, 708
TspRI CASTG 1 cut(s) 850
VpaK11BI GGWCC 2 cut(s) 239, 647
XceI RCATGY 1 cut(s) 789
XmiI GTMKAC 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.