FvH4_6g41430

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000434
Physical Location & Seq
Forward (+)
32574160 .. 32576451
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41430.t1

Sequence Viewer

Length: 1239 bp
ATGGGTCTCAAACTTGTTATGAATCTATTGTCAATACTTATGATCTTATCATCGGCATCTGCAAGAAGAGAGCTTTTATCCGATGGTGAATTTGACGTGACGAGTGCGAAATATGGTGGAAAGGCAGGTTCTGATATTAGTCAGGCTTTAACAAATGCATGGAAAGATGCATGTGCATCGACCTCGCCAAGTAAAATTATTATTCCAAAAGGGACATACCCATTAAAAGAAGCAGAATTCTCAGGACCTTGCAAGGCTCCTATTGAGATGCAACTCCAAGGCACATTGCAGGCACCAGAAGATAGCACCCAATTAACGAAACCAGATACTTGGATTGGGTTTCTATACATTAGCGACTTCACGTTATCCGGTGGTGGAGTCTTTGATGGCCAGGGAGCAAAGGCTTGGAAAGCAAATGATTGCCAGAAAAACCCAAAGTGTTCTAGCACCATTAATATTAGGTTCCACCAGATGAAGAAGTCATTAGTTAAGGATATAACTTCACTTAACAGCAAGAACTTCCATTTCAATATAATAGGTGCCGAGCAACTTACCTTCGACCATGTCACCGTTACTGCACCTGGAGATAGTCCTAACACAGACGGAATTCATATCGGGCGCTCAAATGGGGTCAACATTACCAATTGTGTGATCGGAACTGGAGACGATTGCATTTCAATTGGTGATGGCACCAAGCAACTAACAATAGAGAAGGTAACTTGCGGACCAGGCCATGGAATAAGCGTTGGAAGTCTAGGAAAATACGATAACGAAGAGCCTGTGGCAGGAATCTTTGTTAAGAATTGTACCATCAGTAAAACGCAAAATGGTGTTAGAGTCAAGACATGGCCTAATGGTCCTTCGGCTATCACCGCCTCAGATATTCACTTTGAGGATATCATCATGGATAATGTTCAAAACCCAATCGTGCTAGATCAAGAATACTGCCCATATGATCAGTGTGATAAATCGGCTTCATCAAAAATCAAGATCAGCAATGTGAGTTTCAAAAACATTAGAGGGACGTCTGCCACTGCATTAGGCGTGAAGGTTGCTTGTTCCAAGTCTAACCCTTGTGATCAGGTGGTGATGTCTGACATTGATCTCAAATTATCAGGCGCAGGTGAACTCACTTCACAGTGTACTAATACCAAGCCCACAATTACTAATGTGGCAAAGGCTCTTGCTTGTGCTACTGACCAACCTGTTGATCCTGCAAAGGGTGGAGCACCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

413

Amino Acids

43.68

Weight (kDa)

6.59

Isoelectric Point (pI)

31.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 58 - 385 1.4e-101 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1112
AatII GACGTC 1 cut(s) 1028
Acc36I ACCTGC 2 cut(s) 116, 1112
AccB1I GGYRCC 3 cut(s) 292, 539, 689
AccB7I CCANNNNNTGG 1 cut(s) 734
AciI CCGC 2 cut(s) 723, 873
AclWI GGATC 1 cut(s) 1205
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 3 cut(s) 89, 236, 606
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 2 cut(s) 808, 1144
AfiI CCNNNNNNNGG 3 cut(s) 734, 785, 1220
AgsI TTSAA 4 cut(s) 529, 678, 917, 1009
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 3 cut(s) 390, 580, 727
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Alw21I GWGCWC 1 cut(s) 1231
Alw26I GTCTC 2 cut(s) 11, 657
AlwI GGATC 1 cut(s) 1205
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 3 cut(s) 388, 730, 848
ApoI RAATTY 3 cut(s) 89, 236, 606
AseI ATTAAT 1 cut(s) 453
AspLEI GCGC 2 cut(s) 621, 1121
AspS9I GGNCC 3 cut(s) 245, 725, 857
AsuHPI GGTGA 6 cut(s) 98, 559, 695, 862, 1099, 1136
AvaII GGWCC 3 cut(s) 245, 725, 857
BalI TGGCCA 1 cut(s) 390
BanI GGYRCC 3 cut(s) 292, 539, 689
Bbv12I GWGCWC 1 cut(s) 1231
BccI CCATC 4 cut(s) 77, 380, 680, 818
BcgI CGANNNNNNTGC 4 cut(s) 159, 165, 193, 199
BciT130I CCWGG 3 cut(s) 392, 582, 729
BclI TGATCA 2 cut(s) 955, 1078
BcoDI GTCTC 2 cut(s) 11, 657
BfaI CTAG 3 cut(s) 444, 755, 932
BfoI RGCGCY 1 cut(s) 622
BfuAI ACCTGC 2 cut(s) 116, 1112
Bme1390I CCNGG 3 cut(s) 392, 582, 729
Bme18I GGWCC 3 cut(s) 245, 725, 857
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 3 cut(s) 245, 725, 857
BmiI GGNNCC 5 cut(s) 258, 294, 464, 541, 691
BmrFI CCNGG 3 cut(s) 392, 582, 729
BmsI GCATC 4 cut(s) 65, 157, 185, 258
BpmI CTGGAG 2 cut(s) 603, 681
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 3 cut(s) 277, 391, 733
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 3 cut(s) 734, 785, 1220
Bse1I ACTGG 1 cut(s) 664
Bse3DI GCAATG 2 cut(s) 284, 1003
BseBI CCWGG 3 cut(s) 392, 582, 729
BseDI CCNNGG 3 cut(s) 277, 391, 733
BseLI CCNNNNNNNGG 3 cut(s) 734, 785, 1220
BseMI GCAATG 2 cut(s) 284, 1003
BseMII CTCAG 2 cut(s) 255, 891
BseNI ACTGG 1 cut(s) 664
BsgI GTGCAG 1 cut(s) 561
BshFI GGCC 3 cut(s) 390, 732, 850
BshNI GGYRCC 3 cut(s) 292, 539, 689
BsiHKAI GWGCWC 1 cut(s) 1231
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 2 cut(s) 226, 1036
BslI CCNNNNNNNGG 3 cut(s) 734, 785, 1220
BsmAI GTCTC 2 cut(s) 11, 657
BsmBI CGTCTC 1 cut(s) 657
BsmFI GGGAC 2 cut(s) 226, 1036
BsnI GGCC 3 cut(s) 390, 732, 850
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 1231
Bsp143I GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
Bsp19I CCATGG 1 cut(s) 733
BspACI CCGC 2 cut(s) 723, 873
BspANI GGCC 3 cut(s) 390, 732, 850
BspCNI CTCAG 2 cut(s) 254, 890
BspLI GGNNCC 5 cut(s) 258, 294, 464, 541, 691
BspMI ACCTGC 2 cut(s) 116, 1112
BspPI GGATC 1 cut(s) 1205
BspQI GCTCTTC 1 cut(s) 768
BspT107I GGYRCC 3 cut(s) 292, 539, 689
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 284, 1003
BsrI ACTGG 1 cut(s) 664
BssECI CCNNGG 3 cut(s) 277, 391, 733
BssMI GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
BssNI GRCGYC 1 cut(s) 1025
BssT1I CCWWGG 2 cut(s) 277, 733
Bst2UI CCWGG 3 cut(s) 392, 582, 729
Bst4CI ACNGT 2 cut(s) 571, 1140
Bst6I CTCTTC 2 cut(s) 61, 768
BstACI GRCGYC 1 cut(s) 1025
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 2 cut(s) 241, 877
BstDSI CCRYGG 1 cut(s) 733
BstENI CCTNNNNNAGG 1 cut(s) 783
BstH2I RGCGCY 1 cut(s) 622
BstHHI GCGC 2 cut(s) 621, 1121
BstKTI GATC 8 cut(s) 45, 654, 937, 958, 993, 1081, 1105, 1213
BstMAI GTCTC 2 cut(s) 11, 657
BstMBI GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
BstMWI GCNNNNNNNGC 3 cut(s) 410, 729, 872
BstNI CCWGG 3 cut(s) 392, 582, 729
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 3 cut(s) 390, 580, 727
BstXI CCANNNNNNTGG 1 cut(s) 330
BsuRI GGCC 3 cut(s) 390, 732, 850
BtgI CCRYGG 1 cut(s) 733
BtrI CACGTC 1 cut(s) 97
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 3 cut(s) 965, 1032, 1145
BveI ACCTGC 2 cut(s) 116, 1112
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 2 cut(s) 621, 1121
Cfr13I GGNCC 3 cut(s) 245, 725, 857
Csp6I GTAC 2 cut(s) 807, 1143
CviAII CATG 6 cut(s) 159, 171, 563, 734, 846, 904
CviQI GTAC 2 cut(s) 807, 1143
DdeI CTNAG 2 cut(s) 241, 877
DpnI GATC 8 cut(s) 44, 653, 936, 957, 992, 1080, 1104, 1212
DpnII GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
EaeI YGGCCR 1 cut(s) 388
Eam1104I CTCTTC 2 cut(s) 61, 768
EarI CTCTTC 2 cut(s) 61, 768
Eco130I CCWWGG 2 cut(s) 277, 733
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 898
Eco47I GGWCC 3 cut(s) 245, 725, 857
EcoNI CCTNNNNNAGG 1 cut(s) 783
EcoO109I RGGNCCY 1 cut(s) 245
EcoRI GAATTC 2 cut(s) 236, 606
EcoRII CCWGG 3 cut(s) 390, 580, 727
EcoRV GATATC 1 cut(s) 898
EcoT14I CCWWGG 2 cut(s) 277, 733
EcoT22I ATGCAT 2 cut(s) 160, 172
ErhI CCWWGG 2 cut(s) 277, 733
Esp3I CGTCTC 1 cut(s) 657
FaeI CATG 6 cut(s) 162, 174, 566, 737, 849, 907
FaqI GGGAC 2 cut(s) 226, 1036
FatI CATG 6 cut(s) 158, 170, 562, 733, 845, 903
FauNDI CATATG 1 cut(s) 952
FbaI TGATCA 2 cut(s) 955, 1078
FspBI CTAG 3 cut(s) 444, 755, 932
GlaI GCGC 2 cut(s) 620, 1120
GsuI CTGGAG 2 cut(s) 603, 681
HaeII RGCGCY 1 cut(s) 622
HaeIII GGCC 3 cut(s) 390, 732, 850
HapII CCGG 1 cut(s) 369
HhaI GCGC 2 cut(s) 621, 1121
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 6 cut(s) 162, 174, 566, 737, 849, 907
Hin6I GCGC 2 cut(s) 619, 1119
HinP1I GCGC 2 cut(s) 619, 1119
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 4 cut(s) 22, 378, 789, 837
HpaII CCGG 1 cut(s) 369
HphI GGTGA 6 cut(s) 98, 559, 695, 862, 1099, 1136
Hpy166II GTNNAC 3 cut(s) 634, 1127, 1143
Hpy188I TCNGA 5 cut(s) 82, 133, 656, 880, 1096
Hpy188III TCNNGA 4 cut(s) 243, 841, 938, 988
Hpy8I GTNNAC 3 cut(s) 634, 1127, 1143
HpyAV CCTTC 4 cut(s) 565, 706, 870, 1042
HpyCH4III ACNGT 2 cut(s) 571, 1140
HpyCH4IV ACGT 3 cut(s) 96, 362, 1025
HpyF10VI GCNNNNNNNGC 3 cut(s) 410, 729, 872
HpyF3I CTNAG 2 cut(s) 241, 877
HpySE526I ACGT 3 cut(s) 96, 362, 1025
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 6 cut(s) 162, 174, 566, 737, 849, 907
HspAI GCGC 2 cut(s) 619, 1119
Ksp22I TGATCA 2 cut(s) 955, 1078
Kzo9I GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
LguI GCTCTTC 1 cut(s) 768
LmnI GCTCC 3 cut(s) 262, 395, 1226
LweI GCATC 4 cut(s) 65, 157, 185, 258
MaeI CTAG 3 cut(s) 444, 755, 932
MaeII ACGT 3 cut(s) 96, 362, 1025
MaeIII GTNAC 4 cut(s) 97, 565, 571, 715
MalI GATC 8 cut(s) 44, 653, 936, 957, 992, 1080, 1104, 1212
MboI GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
MboII GAAGA 4 cut(s) 78, 311, 487, 785
MfeI CAATTG 2 cut(s) 643, 678
MhlI GDGCHC 1 cut(s) 1231
MlsI TGGCCA 1 cut(s) 390
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 2 cut(s) 387, 846
MmeI TCCRAC 1 cut(s) 727
MnlI CCTC 4 cut(s) 193, 886, 886, 1013
Mox20I TGGCCA 1 cut(s) 390
Mph1103I ATGCAT 2 cut(s) 160, 172
MscI TGGCCA 1 cut(s) 390
MseI TTAA 7 cut(s) 149, 224, 314, 453, 489, 507, 798
Msp20I TGGCCA 1 cut(s) 390
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 3 cut(s) 392, 582, 729
MunI CAATTG 2 cut(s) 643, 678
MvaI CCWGG 3 cut(s) 392, 582, 729
MwoI GCNNNNNNNGC 3 cut(s) 410, 729, 872
NcoI CCATGG 1 cut(s) 733
NdeI CATATG 1 cut(s) 952
NdeII GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
NlaIII CATG 6 cut(s) 162, 174, 566, 737, 849, 907
NlaIV GGNNCC 5 cut(s) 258, 294, 464, 541, 691
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 2 cut(s) 97, 565
NsiI ATGCAT 2 cut(s) 160, 172
NspI RCATGY 1 cut(s) 174
PaqCI CACCTGC 1 cut(s) 1112
PciSI GCTCTTC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 22, 789
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 734
PleI GAGTC 2 cut(s) 386, 845
PpsI GAGTC 2 cut(s) 386, 845
PpuMI RGGWCCY 1 cut(s) 245
PshBI ATTAAT 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 3 cut(s) 390, 580, 727
PspGI CCWGG 3 cut(s) 390, 580, 727
PspN4I GGNNCC 5 cut(s) 258, 294, 464, 541, 691
PspPI GGNCC 3 cut(s) 245, 725, 857
PspPPI RGGWCCY 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 131
PsyI GACNNNGTC 1 cut(s) 563
RsaI GTAC 2 cut(s) 808, 1144
RsaNI GTAC 2 cut(s) 807, 1143
SapI GCTCTTC 1 cut(s) 768
SaqAI TTAA 7 cut(s) 149, 224, 314, 453, 489, 507, 798
Sau3AI GATC 8 cut(s) 42, 651, 934, 955, 990, 1078, 1102, 1210
Sau96I GGNCC 3 cut(s) 245, 725, 857
SchI GAGTC 2 cut(s) 387, 846
ScrFI CCNGG 3 cut(s) 392, 582, 729
SduI GDGCHC 1 cut(s) 1231
SfaNI GCATC 4 cut(s) 65, 157, 185, 258
SinI GGWCC 3 cut(s) 245, 725, 857
SsiI CCGC 2 cut(s) 723, 873
SspI AATATT 1 cut(s) 457
SspMI CTAG 3 cut(s) 444, 755, 932
StyD4I CCNGG 3 cut(s) 390, 580, 727
StyI CCWWGG 2 cut(s) 277, 733
TaaI ACNGT 2 cut(s) 571, 1140
TaiI ACGT 3 cut(s) 99, 365, 1028
TaqI TCGA 2 cut(s) 179, 558
TatI WGTACW 1 cut(s) 1142
TfiI GAWTC 2 cut(s) 22, 789
Tru1I TTAA 7 cut(s) 149, 224, 314, 453, 489, 507, 798
Tru9I TTAA 7 cut(s) 149, 224, 314, 453, 489, 507, 798
TscAI CASTG 3 cut(s) 965, 1039, 1145
TseFI GTSAC 2 cut(s) 97, 565
Tsp45I GTSAC 2 cut(s) 97, 565
TspDTI ATGAA 4 cut(s) 35, 488, 599, 966
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 3 cut(s) 965, 1039, 1145
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 3 cut(s) 245, 725, 857
VspI ATTAAT 1 cut(s) 453
XagI CCTNNNNNAGG 1 cut(s) 783
XapI RAATTY 3 cut(s) 89, 236, 606
XceI RCATGY 1 cut(s) 174
XspI CTAG 3 cut(s) 444, 755, 932
ZraI GACGTC 1 cut(s) 1026
Zsp2I ATGCAT 2 cut(s) 160, 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.