FvH4_6g43060

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000434
Physical Location & Seq
Forward (+)
33362641 .. 33364562
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43060.t1

Sequence Viewer

Length: 1188 bp
ATGGGTCTTAAAGTGATGAATATCATGGCGATATTCATGTTCTTGTTGCTAGCAGCTACAGCTAATGCCCAGCCCCGTACCATCTTTGACGTGACGAGCGCAACATATGGTGGAAAGCCTAATTGTGATATTACAGATGCCTTAGCACACGCTTGGAGAGATGCTTGTGCTTCACCATGGCCGAGCAGACTCGTTGTTCCAAGCGGCACATACCGGTTAAGAGGAGCAAACTTTAAAGGTCCTTGCAACGCTCCTATTGAGGTGCAAATCCAAGGAACGTTGCAAGCTCCCGAAGATGGCCACCAACTCGTGAACTCGGATTCCTGGGTTGCTTTTCAGTACATTGACAGGCTCTTCTTAACAGGAGGAGGAACTTTTGATGGCTCCCAAGGTCGGAATAATTATCGTGCCATTAACGTGAGGTTCGACTTCGTCACCAACTCGGCAATTCAGGACATAACTTCCCTTGACAGCTCAGGTTTCCACTTCAACGTTTTTATGTGCCATGATCTTACATTCCAGCGTTTAACCATCCAAGCACCAAAGAAGAGCTCGAATACAGATGGAATCCACATTGCACGTTCATGGAGAATCAACGTAACCGATACAGACATCGGAACCGGAGATGATTGTATCTCCATCGGTGATGGCACTAAGAAGCTCATAGTAACCAATGTTAATTGCGGACCAGGCCATGGAATAAGCGTTGGAAGTCTTGGAAAGTATAGTGATGAACAACCGGTGTATGGCCTCGTTGTCAGGAACTGCACCTTCACGAACACAACAAATGGTGTGAGGATCAAGACATGGCCGGGGTCTCCCGTTACCGGTTTGGCCTCACACATACACTTCGTGGATATTACAATGAACAATGTGCAGAACCCCATCATAATAGACCAAAACTACTGCCCAGGGCATACTTGCAATCAACAGGGTCAGTCGAACATTAAGATTAGTAACATCAGATTCAGGAACATCAGAGGCACATCTGCATCTCAAGTTGCCGTCAACCTCGCATGTAGTCAGACCTTACCATGTGAGATGGTGGAACTGATAGGCATTGATCTCGCATACAGTGGAGAACACAATCGACCTATCTTATCTCAATGTACTAATGTCGTCAATCCCACAATTGTTAACGTGAGGGACGCTCTTGCTTGCAAAGTGCAGACTACCGAACTAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

396

Amino Acids

42.99

Weight (kDa)

6.78

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 375 3.1e-89 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 695
AciI CCGC 2 cut(s) 204, 684
AclI AACGTT 2 cut(s) 278, 492
AclWI GGATC 1 cut(s) 806
AcoI YGGCCR 3 cut(s) 179, 298, 809
AdeI CACNNNGTG 1 cut(s) 853
AfaI GTAC 3 cut(s) 79, 341, 1111
AfiI CCNNNNNNNGG 5 cut(s) 296, 393, 695, 746, 827
AgeI ACCGGT 3 cut(s) 213, 739, 827
AgsI TTSAA 1 cut(s) 490
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 3 cut(s) 323, 688, 910
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
AluBI AGCT 6 cut(s) 56, 62, 287, 474, 552, 661
AluI AGCT 6 cut(s) 56, 62, 287, 474, 552, 661
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 1 cut(s) 822
AlwI GGATC 1 cut(s) 806
AlwNI CAGNNNCTG 1 cut(s) 765
AoxI GGCC 6 cut(s) 179, 298, 691, 748, 809, 834
ApeKI GCWGC 1 cut(s) 53
AsiGI ACCGGT 3 cut(s) 213, 739, 827
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 239, 686
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 3 cut(s) 165, 427, 656
AsuNHI GCTAGC 1 cut(s) 49
AvaII GGWCC 2 cut(s) 239, 686
BalI TGGCCA 1 cut(s) 300
BanII GRGCYC 1 cut(s) 554
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 1 cut(s) 65
BccI CCATC 9 cut(s) 89, 290, 374, 539, 557, 641, 647, 893, 1036
BceAI ACGGC 1 cut(s) 989
BcgI CGANNNNNNTGC 2 cut(s) 1048, 1082
BciT130I CCWGG 3 cut(s) 325, 690, 912
BcnI CCSGG 1 cut(s) 813
BcoDI GTCTC 1 cut(s) 822
BfaI CTAG 2 cut(s) 50, 1181
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 2 cut(s) 54, 205
BlsI GCNGC 2 cut(s) 55, 206
Bme1390I CCNGG 4 cut(s) 325, 690, 813, 912
Bme18I GGWCC 2 cut(s) 239, 686
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 239, 686
BmiI GGNNCC 2 cut(s) 385, 619
BmrFI CCNGG 4 cut(s) 325, 690, 813, 912
BmsI GCATC 3 cut(s) 127, 151, 1001
BmtI GCTAGC 1 cut(s) 53
BplI GAGNNNNNCTC 2 cut(s) 1135, 1167
Bpu10I CCTNAGC 2 cut(s) 142, 475
BpuEI CTTGAG 1 cut(s) 981
BpuMI CCSGG 1 cut(s) 813
BsaBI GATNNNNATC 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 822
BsaJI CCNNGG 8 cut(s) 176, 271, 324, 388, 694, 812, 910, 911
BsaWI WCCGGW 4 cut(s) 213, 620, 739, 827
Bsc4I CCNNNNNNNGG 5 cut(s) 296, 393, 695, 746, 827
Bse118I RCCGGY 3 cut(s) 213, 739, 827
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 3 cut(s) 325, 690, 912
BseDI CCNNGG 8 cut(s) 176, 271, 324, 388, 694, 812, 910, 911
BseGI GGATG 1 cut(s) 531
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 5 cut(s) 296, 393, 695, 746, 827
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 237, 381
BseXI GCAGC 1 cut(s) 65
BseYI CCCAGC 1 cut(s) 69
BsgI GTGCAG 3 cut(s) 751, 896, 1187
BshFI GGCC 6 cut(s) 181, 300, 693, 750, 811, 836
BshTI ACCGGT 3 cut(s) 213, 739, 827
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 5 cut(s) 214, 621, 740, 812, 828
BslFI GGGAC 1 cut(s) 1160
BslI CCNNNNNNNGG 5 cut(s) 296, 393, 695, 746, 827
BsmAI GTCTC 1 cut(s) 822
BsmFI GGGAC 1 cut(s) 1160
BsnI GGCC 6 cut(s) 181, 300, 693, 750, 811, 836
Bso31I GGTCTC 1 cut(s) 822
Bsp1286I GDGCHC 1 cut(s) 554
Bsp143I GATC 3 cut(s) 508, 798, 1063
Bsp19I CCATGG 2 cut(s) 176, 694
BspACI CCGC 2 cut(s) 204, 684
BspANI GGCC 6 cut(s) 181, 300, 693, 750, 811, 836
BspCNI CTCAG 1 cut(s) 488
BspLI GGNNCC 2 cut(s) 385, 619
BspOI GCTAGC 1 cut(s) 53
BspPI GGATC 1 cut(s) 806
BspQI GCTCTTC 2 cut(s) 359, 542
BspTNI GGTCTC 1 cut(s) 822
BsrDI GCAATG 1 cut(s) 573
BsrFI RCCGGY 3 cut(s) 213, 739, 827
BssAI RCCGGY 3 cut(s) 213, 739, 827
BssECI CCNNGG 8 cut(s) 176, 271, 324, 388, 694, 812, 910, 911
BssMI GATC 3 cut(s) 508, 798, 1063
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 4 cut(s) 176, 271, 388, 694
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 3 cut(s) 325, 690, 912
Bst4CI ACNGT 1 cut(s) 1076
Bst6I CTCTTC 2 cut(s) 359, 542
BstC8I GCNNGC 3 cut(s) 51, 285, 1159
BstDEI CTNAG 3 cut(s) 142, 475, 654
BstDSI CCRYGG 2 cut(s) 176, 694
BstF5I GGATG 1 cut(s) 531
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 3 cut(s) 511, 801, 1066
BstMAI GTCTC 1 cut(s) 822
BstMBI GATC 3 cut(s) 508, 798, 1063
BstMWI GCNNNNNNNGC 2 cut(s) 59, 690
BstNI CCWGG 3 cut(s) 325, 690, 912
BstNSI RCATGY 1 cut(s) 1020
BstSCI CCNGG 4 cut(s) 323, 688, 811, 910
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 65
BsuRI GGCC 6 cut(s) 181, 300, 693, 750, 811, 836
BtgI CCRYGG 2 cut(s) 176, 694
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 1081
Cac8I GCNNGC 3 cut(s) 51, 285, 1159
CaiI CAGNNNCTG 1 cut(s) 765
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 3 cut(s) 213, 739, 827
Cfr13I GGNCC 2 cut(s) 239, 686
CseI GACGC 1 cut(s) 1157
Csp6I GTAC 3 cut(s) 78, 340, 1110
CspAI ACCGGT 3 cut(s) 213, 739, 827
CviAII CATG 9 cut(s) 25, 37, 177, 506, 585, 695, 807, 1017, 1035
CviQI GTAC 3 cut(s) 78, 340, 1110
DdeI CTNAG 3 cut(s) 142, 475, 654
DpnI GATC 3 cut(s) 510, 800, 1065
DpnII GATC 3 cut(s) 508, 798, 1063
DraI TTTAAA 1 cut(s) 235
DraIII CACNNNGTG 1 cut(s) 853
EaeI YGGCCR 3 cut(s) 179, 298, 809
Eam1104I CTCTTC 2 cut(s) 359, 542
EarI CTCTTC 2 cut(s) 359, 542
Ecl136II GAGCTC 1 cut(s) 552
Eco130I CCWWGG 4 cut(s) 176, 271, 388, 694
Eco24I GRGCYC 1 cut(s) 554
Eco31I GGTCTC 1 cut(s) 822
Eco47I GGWCC 2 cut(s) 239, 686
Eco53kI GAGCTC 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 3 cut(s) 323, 688, 910
EcoT14I CCWWGG 4 cut(s) 176, 271, 388, 694
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 4 cut(s) 176, 271, 388, 694
FaeI CATG 9 cut(s) 28, 40, 180, 509, 588, 698, 810, 1020, 1038
FaqI GGGAC 1 cut(s) 1160
FatI CATG 9 cut(s) 24, 36, 176, 505, 584, 694, 806, 1016, 1034
FauNDI CATATG 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 54, 205
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 2 cut(s) 54, 205
FspBI CTAG 2 cut(s) 50, 1181
GlaI GCGC 1 cut(s) 100
GluI GCNGC 2 cut(s) 54, 205
GsaI CCCAGC 1 cut(s) 73
HaeIII GGCC 6 cut(s) 181, 300, 693, 750, 811, 836
HapII CCGG 5 cut(s) 214, 621, 740, 812, 828
HgaI GACGC 1 cut(s) 1157
HhaI GCGC 1 cut(s) 101
Hin1II CATG 9 cut(s) 28, 40, 180, 509, 588, 698, 810, 1020, 1038
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 2 cut(s) 1009, 1138
HindII GTYRAC 2 cut(s) 1009, 1138
HinfI GANTC 5 cut(s) 189, 320, 567, 591, 966
HpaI GTTAAC 1 cut(s) 1138
HpaII CCGG 5 cut(s) 214, 621, 740, 812, 828
HphI GGTGA 3 cut(s) 165, 427, 656
Hpy166II GTNNAC 3 cut(s) 313, 1009, 1138
Hpy188I TCNGA 6 cut(s) 319, 396, 617, 965, 980, 1026
Hpy188III TCNNGA 7 cut(s) 290, 310, 452, 760, 775, 802, 970
Hpy8I GTNNAC 3 cut(s) 313, 1009, 1138
HpyAV CCTTC 1 cut(s) 781
HpyCH4III ACNGT 1 cut(s) 1076
HpyCH4IV ACGT 7 cut(s) 90, 278, 417, 492, 580, 597, 1140
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 690
HpyF3I CTNAG 3 cut(s) 142, 475, 654
HpySE526I ACGT 7 cut(s) 90, 278, 417, 492, 580, 597, 1140
Hsp92II CATG 9 cut(s) 28, 40, 180, 509, 588, 698, 810, 1020, 1038
HspAI GCGC 1 cut(s) 99
KspAI GTTAAC 1 cut(s) 1138
Kzo9I GATC 3 cut(s) 508, 798, 1063
LguI GCTCTTC 2 cut(s) 359, 542
LmnI GCTCC 4 cut(s) 224, 256, 292, 389
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 3 cut(s) 127, 151, 1001
MaeI CTAG 2 cut(s) 50, 1181
MaeII ACGT 7 cut(s) 90, 278, 417, 492, 580, 597, 1140
MaeIII GTNAC 6 cut(s) 91, 433, 598, 667, 823, 956
MalI GATC 3 cut(s) 510, 800, 1065
MboI GATC 3 cut(s) 508, 798, 1063
MboII GAAGA 3 cut(s) 305, 346, 559
MfeI CAATTG 1 cut(s) 1131
MhlI GDGCHC 1 cut(s) 554
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 5 cut(s) 121, 400, 447, 679, 1131
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 2 cut(s) 374, 688
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MslI CAYNNNNRTG 2 cut(s) 416, 583
Msp20I TGGCCA 1 cut(s) 300
MspI CCGG 5 cut(s) 214, 621, 740, 812, 828
MspR9I CCNGG 4 cut(s) 325, 690, 813, 912
MunI CAATTG 1 cut(s) 1131
MvaI CCWGG 3 cut(s) 325, 690, 912
MwoI GCNNNNNNNGC 2 cut(s) 59, 690
NciI CCSGG 1 cut(s) 813
NcoI CCATGG 2 cut(s) 176, 694
NdeI CATATG 1 cut(s) 106
NdeII GATC 3 cut(s) 508, 798, 1063
NheI GCTAGC 1 cut(s) 49
NlaIII CATG 9 cut(s) 28, 40, 180, 509, 588, 698, 810, 1020, 1038
NlaIV GGNNCC 2 cut(s) 385, 619
NmeAIII GCCGAG 2 cut(s) 207, 422
NmuCI GTSAC 2 cut(s) 91, 433
NspI RCATGY 1 cut(s) 1020
PasI CCCWGGG 1 cut(s) 911
PciSI GCTCTTC 2 cut(s) 359, 542
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PfeI GAWTC 4 cut(s) 320, 567, 591, 966
PflFI GACNNNGTC 1 cut(s) 431
PflMI CCANNNNNTGG 1 cut(s) 695
PinAI ACCGGT 3 cut(s) 213, 739, 827
PkrI GCNGC 2 cut(s) 55, 206
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PpuMI RGGWCCY 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 554
Psp1406I AACGTT 2 cut(s) 278, 492
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 3 cut(s) 323, 688, 910
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 3 cut(s) 323, 688, 910
PspN4I GGNNCC 2 cut(s) 385, 619
PspPI GGNCC 2 cut(s) 239, 686
PspPPI RGGWCCY 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 765
PsyI GACNNNGTC 1 cut(s) 431
RsaI GTAC 3 cut(s) 79, 341, 1111
RsaNI GTAC 3 cut(s) 78, 340, 1110
RseI CAYNNNNRTG 2 cut(s) 416, 583
SacI GAGCTC 1 cut(s) 554
SapI GCTCTTC 2 cut(s) 359, 542
SatI GCNGC 2 cut(s) 54, 205
Sau3AI GATC 3 cut(s) 508, 798, 1063
Sau96I GGNCC 2 cut(s) 239, 686
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 4 cut(s) 325, 690, 813, 912
SduI GDGCHC 1 cut(s) 554
SfaNI GCATC 3 cut(s) 127, 151, 1001
SfcI CTRYAG 1 cut(s) 57
SinI GGWCC 2 cut(s) 239, 686
SmiMI CAYNNNNRTG 2 cut(s) 416, 583
SmlI CTYRAG 1 cut(s) 996
SmoI CTYRAG 1 cut(s) 996
Sse9I AATT 5 cut(s) 121, 400, 447, 679, 1131
SsiI CCGC 2 cut(s) 204, 684
SspMI CTAG 2 cut(s) 50, 1181
SstI GAGCTC 1 cut(s) 554
StyD4I CCNGG 4 cut(s) 323, 688, 811, 910
StyI CCWWGG 4 cut(s) 176, 271, 388, 694
TaaI ACNGT 1 cut(s) 1076
TaiI ACGT 7 cut(s) 93, 281, 420, 495, 583, 600, 1143
TaqI TCGA 4 cut(s) 426, 554, 941, 1090
TasI AATT 5 cut(s) 121, 400, 447, 679, 1131
TatI WGTACW 2 cut(s) 339, 1109
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 4 cut(s) 320, 567, 591, 966
TscAI CASTG 1 cut(s) 1081
TseFI GTSAC 2 cut(s) 91, 433
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 2 cut(s) 91, 433
TspDTI ATGAA 5 cut(s) 25, 32, 573, 747, 881
TspRI CASTG 1 cut(s) 1081
Tth111I GACNNNGTC 1 cut(s) 431
Van91I CCANNNNNTGG 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 239, 686
XceI RCATGY 1 cut(s) 1020
XspI CTAG 2 cut(s) 50, 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.