MD16G1223500.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000434
Physical Location & Seq
Reverse (-)
22486961 .. 22487818
858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1223500.v1.1.491

Sequence Viewer

Length: 858 bp
ATGCTCACTGTATCAGGTGGTGGAACTTTTGACGGGCAAGGAGCACTTGCTTGGAAGCAAAATGACTGCAACATAAACAAAGATTGCAAATCTATTGCCATTAATATAAGGTTCAACTTCGTTACCAATTCCATAATTCAGGACATAACTTCCCTCGATAGCAAGAGTTTTCACATCAACATTTTGGGGTGTAACAATGTCAATTTCCACTGTGTTAATGTCATAGCACCTGGAGTGAGCATAAATACAGATGGAATCCATATTGGGCATTCAACTGGAATCAACATTACTGATGCACACATTAGGACTGGTGATGACTGTGTTTCAATTGGTGATGGAAACAAAGAAATCAATGTGACTAGGGTTACTTGTGGACTATGCCATGGCATAAGCATTGAAAGTCTTGGAAGGTACCCGAACGAAGAACCTGTGGTTGGAATCAGAGTTAAAAACTGTACCTTGACTAATACCTCCAACGGCGTGAGAATTAAAACATGGCCTGCTTCCCCTTCGGATGGTATTGCATCAGATATGCACTTTGATGACATTATCATGGTCAATGTCAGCAACCCTATCCTCATAGACCAACAGTATTGCCCGTGGAACCAGTGTAAACAGAACGTTGCTTCGAAAGTTAAGATCAACAATGTCAGCTTCAAGAACATTAAGGGCTCATCTGCAACACCACTTGCAGTTCAGATTGTATGCAGCAGTGGCATACCGTGTGAGAATGTGGAATTGGTTGACATCGATCTCACCTACAATGGAAACAAAGGACCTCTTACCTCTGAATGTTCAAACGTGAAGCCCACCATTACACGAGTGCCTAAGGCTCTTGCGTGTGCAACATCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

286

Amino Acids

30.47

Weight (kDa)

6.36

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 2 - 270 7.2e-82 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 411
AclI AACGTT 1 cut(s) 621
AfaI GTAC 2 cut(s) 413, 457
AfiI CCNNNNNNNGG 2 cut(s) 434, 515
AgsI TTSAA 6 cut(s) 115, 273, 327, 398, 658, 798
AjnI CCWGG 1 cut(s) 229
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 722, 754
AluBI AGCT 1 cut(s) 654
AluI AGCT 1 cut(s) 654
Alw21I GWGCWC 1 cut(s) 46
AoxI GGCC 1 cut(s) 497
ApeKI GCWGC 1 cut(s) 708
AseI ATTAAT 1 cut(s) 102
Asp718I GGTACC 1 cut(s) 411
AspS9I GGNCC 1 cut(s) 776
AsuHPI GGTGA 3 cut(s) 323, 344, 748
AsuII TTCGAA 1 cut(s) 629
AvaII GGWCC 1 cut(s) 776
AxyI CCTNAGG 1 cut(s) 828
BanI GGYRCC 1 cut(s) 411
BanII GRGCYC 1 cut(s) 674
BauI CACGAG 1 cut(s) 819
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 720
BccI CCATC 3 cut(s) 245, 329, 509
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 1 cut(s) 231
BfaI CTAG 1 cut(s) 360
BisI GCNGC 1 cut(s) 709
BlsI GCNGC 1 cut(s) 710
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 776
BmgT120I GGNCC 1 cut(s) 776
BmiI GGNNCC 2 cut(s) 413, 605
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 2 cut(s) 283, 533
BpmI CTGGAG 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 629
Bsa29I ATCGAT 1 cut(s) 750
BsaJI CCNNGG 2 cut(s) 382, 599
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 515
Bse1I ACTGG 3 cut(s) 280, 313, 607
Bse21I CCTNAGG 1 cut(s) 828
BseBI CCWGG 1 cut(s) 231
BseCI ATCGAT 1 cut(s) 750
BseDI CCNNGG 2 cut(s) 382, 599
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 434, 515
BseNI ACTGG 3 cut(s) 280, 313, 607
BseXI GCAGC 1 cut(s) 720
BshFI GGCC 1 cut(s) 499
BshNI GGYRCC 1 cut(s) 411
BshVI ATCGAT 1 cut(s) 750
BsiHKAI GWGCWC 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 434, 515
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 1 cut(s) 499
Bsp119I TTCGAA 1 cut(s) 629
Bsp1286I GDGCHC 2 cut(s) 46, 674
Bsp143I GATC 2 cut(s) 639, 751
Bsp19I CCATGG 1 cut(s) 382
BspANI GGCC 1 cut(s) 499
BspDI ATCGAT 1 cut(s) 750
BspLI GGNNCC 2 cut(s) 413, 605
BspT104I TTCGAA 1 cut(s) 629
BspT107I GGYRCC 1 cut(s) 411
BsrI ACTGG 3 cut(s) 280, 313, 607
BssECI CCNNGG 2 cut(s) 382, 599
BssMI GATC 2 cut(s) 639, 751
BssSI CACGAG 1 cut(s) 819
BssT1I CCWWGG 1 cut(s) 382
Bst2BI CACGAG 1 cut(s) 819
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 6 cut(s) 10, 212, 320, 455, 591, 723
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 629
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 1 cut(s) 828
BstDSI CCRYGG 2 cut(s) 382, 599
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 2 cut(s) 642, 754
BstMBI GATC 2 cut(s) 639, 751
BstMWI GCNNNNNNNGC 2 cut(s) 714, 851
BstNI CCWGG 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 720
Bsu15I ATCGAT 1 cut(s) 750
Bsu36I CCTNAGG 1 cut(s) 828
BsuRI GGCC 1 cut(s) 499
BsuTUI ATCGAT 1 cut(s) 750
BtgI CCRYGG 2 cut(s) 382, 599
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 4 cut(s) 6, 208, 614, 718
Cac8I GCNNGC 1 cut(s) 501
Cfr13I GGNCC 1 cut(s) 776
ClaI ATCGAT 1 cut(s) 750
Csp6I GTAC 2 cut(s) 412, 456
CviAII CATG 3 cut(s) 383, 495, 553
CviJI RGCY 5 cut(s) 499, 654, 672, 808, 833
CviKI_1 RGCY 5 cut(s) 499, 654, 672, 808, 833
CviQI GTAC 2 cut(s) 412, 456
DdeI CTNAG 1 cut(s) 828
DpnI GATC 2 cut(s) 641, 753
DpnII GATC 2 cut(s) 639, 751
Eco130I CCWWGG 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 674
Eco47I GGWCC 1 cut(s) 776
Eco81I CCTNAGG 1 cut(s) 828
EcoO109I RGGNCCY 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 382
EcoT38I GRGCYC 1 cut(s) 674
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 3 cut(s) 386, 498, 556
FalI AAGNNNNNCTT 4 cut(s) 30, 62, 765, 797
FatI CATG 3 cut(s) 382, 494, 552
Fnu4HI GCNGC 1 cut(s) 709
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 1 cut(s) 709
FspBI CTAG 1 cut(s) 360
GluI GCNGC 1 cut(s) 709
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 1 cut(s) 499
Hin1II CATG 3 cut(s) 386, 498, 556
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HinfI GANTC 3 cut(s) 255, 279, 438
HphI GGTGA 3 cut(s) 323, 344, 748
Hpy166II GTNNAC 3 cut(s) 374, 614, 745
Hpy188I TCNGA 5 cut(s) 443, 514, 529, 699, 790
Hpy188III TCNNGA 2 cut(s) 140, 658
Hpy8I GTNNAC 3 cut(s) 374, 614, 745
HpyAV CCTTC 2 cut(s) 402, 519
HpyCH4III ACNGT 6 cut(s) 10, 212, 320, 455, 591, 723
HpyCH4IV ACGT 2 cut(s) 621, 801
HpyCH4V TGCA 9 cut(s) 69, 87, 296, 524, 535, 680, 692, 708, 845
HpyF10VI GCNNNNNNNGC 2 cut(s) 714, 851
HpyF3I CTNAG 1 cut(s) 828
HpySE526I ACGT 2 cut(s) 621, 801
Hsp92II CATG 3 cut(s) 386, 498, 556
KpnI GGTACC 1 cut(s) 415
Kzo9I GATC 2 cut(s) 639, 751
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 8 cut(s) 125, 216, 243, 261, 294, 441, 513, 620
Lsp1109I GCAGC 1 cut(s) 720
LweI GCATC 2 cut(s) 283, 533
MaeI CTAG 1 cut(s) 360
MaeII ACGT 2 cut(s) 621, 801
MaeIII GTNAC 4 cut(s) 121, 191, 355, 364
MalI GATC 2 cut(s) 641, 753
MboI GATC 2 cut(s) 639, 751
MboII GAAGA 1 cut(s) 434
MfeI CAATTG 1 cut(s) 327
MhlI GDGCHC 2 cut(s) 46, 674
MluCI AATT 6 cut(s) 127, 135, 202, 327, 486, 737
MmeI TCCRAC 2 cut(s) 415, 498
MnlI CCTC 5 cut(s) 164, 481, 587, 789, 796
MseI TTAA 6 cut(s) 102, 216, 447, 489, 636, 666
MslI CAYNNNNRTG 2 cut(s) 540, 551
MspR9I CCNGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 714, 851
NcoI CCATGG 1 cut(s) 382
NdeII GATC 2 cut(s) 639, 751
NlaIII CATG 3 cut(s) 386, 498, 556
NlaIV GGNNCC 2 cut(s) 413, 605
NmuCI GTSAC 1 cut(s) 355
NspV TTCGAA 1 cut(s) 629
PctI GAATGC 1 cut(s) 268
PfeI GAWTC 3 cut(s) 255, 279, 438
PkrI GCNGC 1 cut(s) 710
PpuMI RGGWCCY 1 cut(s) 776
PshBI ATTAAT 1 cut(s) 102
Psp1406I AACGTT 1 cut(s) 621
Psp5II RGGWCCY 1 cut(s) 776
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 413, 605
PspPI GGNCC 1 cut(s) 776
PspPPI RGGWCCY 1 cut(s) 776
RsaI GTAC 2 cut(s) 413, 457
RsaNI GTAC 2 cut(s) 412, 456
RseI CAYNNNNRTG 2 cut(s) 540, 551
SaqAI TTAA 6 cut(s) 102, 216, 447, 489, 636, 666
SatI GCNGC 1 cut(s) 709
Sau3AI GATC 2 cut(s) 639, 751
Sau96I GGNCC 1 cut(s) 776
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 2 cut(s) 46, 674
SfaNI GCATC 2 cut(s) 283, 533
SfuI TTCGAA 1 cut(s) 629
SinI GGWCC 1 cut(s) 776
SmiMI CAYNNNNRTG 2 cut(s) 540, 551
Sse9I AATT 6 cut(s) 127, 135, 202, 327, 486, 737
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 6 cut(s) 10, 212, 320, 455, 591, 723
TaiI ACGT 2 cut(s) 624, 804
TaqI TCGA 3 cut(s) 156, 629, 750
TasI AATT 6 cut(s) 127, 135, 202, 327, 486, 737
TfiI GAWTC 3 cut(s) 255, 279, 438
Tru1I TTAA 6 cut(s) 102, 216, 447, 489, 636, 666
Tru9I TTAA 6 cut(s) 102, 216, 447, 489, 636, 666
TscAI CASTG 4 cut(s) 13, 215, 614, 718
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 1 cut(s) 708
Tsp45I GTSAC 1 cut(s) 355
TspRI CASTG 4 cut(s) 13, 215, 614, 718
VpaK11BI GGWCC 1 cut(s) 776
VspI ATTAAT 1 cut(s) 102
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.