Rroxscaffold_4G00326280

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000434
Physical Location & Seq
Forward (+)
58314508 .. 58316968
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326280.1

Sequence Viewer

Length: 1359 bp
ATGAAATTTCCGGGGAAGGAAGATCCGAGTCCCAACCATGGAGTGGCGTCACAGGACGACGTAGAGGCTGGGCTTGATTCGTTGGACGCTAGCGGAGAAGAAGGCGGTGGCGGTGCACCGCGAGGTGTCATTGTGGTGGCGGATGGGGTGTTAGTGGCCGGCTGCGCTCCCAAGTTTGATCATGGGTTTTGGTTTTTACTTGGAGGTAGATCTAGGGAGCTTTTAGCCGGTGCTGGCTTTGACGTGACCACTGCAAAATATGGTGCAACGCCCAATTCTGATATTACTCGGGCTTTAGCAAATGCATGGAAAGATGCATGTGCATCAACATCGGCAAGTAAAGTTATTATTCCAAAAGGGACATACAAACTGAAAGAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCTATTGAGGTTCAGGTCCAAGGTACTTTGCAGGCGCCACCAGACGGCGCCCAACTATCGAAACCAGATACGTGGATCGAGTTTTCATACCTTGACAGGTTCACATTATCGGGTAGTGGAACCTTTGATGGCCAAGGACAAAAAGCTTGGAAAGAAAATGATTGCCACAAAAACCCAAAGTGTTCATCTATTACCGTTAATATGAGGTTCCACTCTGTCAAGAACTCATTAGTCAGGGATGTAACTTCACTTAACAGCAAGAATTTCCACGTGAATGTATTATCTTGCGAGCGACTTACATTCCAACATTTCACTGTCGCTGCACCAGGAGATAGCGTTAATACAGATGGAATACATATTGGGCGCTCAACCGGGGTCAATATTACTGATTCAAAGATTGGTACAGGGGACGATTGCATTTCAATTGGTGATGGCACCAAGCAACTAACTATAACTAAGGTAACTTGTGGACCAGGCCATGGAATAAGCATAGGAAGTCTTGGAAGGTACCCAAAAGAAGAACCTGTGAGTGGAATCCATATTAGGAACTGTACCCTCAGTAATACGTCCAACGGTGTTAGAATCAAGACATGGCCTAATTCTCCTGCAGCTAGCACTGCCTCGGATATTCACTTTGAGGATATCATCATGAATAATGTTGGTAACCCAATCCTCATTGATCAAGAATACTGCCCATGGAATCAGTGTAATAAACAGGCTTCATCAAAAATCAAGATCAGCAATGTGAGCTTCAAGAACATTAGAGGGACGTCTTCCACTCCGGTGGCCGTGAAGATTGCATGCGCCAAGGGCTTACCATGTGACAAGGTGGAGATGACTGACATTAATCTCAAGTACAGTGGAAAGCAAGGCTCTATTACATCTCAATGCTCTAATGTGAGGCCCACAATTACTCGAGTCGCAAATACTCTTGCTTGTGCTACCAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

452

Amino Acids

47.92

Weight (kDa)

8.53

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 107 - 436 1.4e-106 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1178
Acc65I GGTACC 1 cut(s) 912
AccB1I GGYRCC 4 cut(s) 439, 452, 839, 912
AccB7I CCANNNNNTGG 2 cut(s) 43, 884
AccII CGCG 1 cut(s) 121
AciI CCGC 5 cut(s) 93, 105, 111, 119, 140
AclWI GGATC 2 cut(s) 17, 488
AcoI YGGCCR 3 cut(s) 156, 535, 1191
AcsI RAATTY 2 cut(s) 5, 667
AcvI CACGTG 1 cut(s) 676
AcyI GRCGYC 4 cut(s) 47, 440, 453, 1175
AfaI GTAC 5 cut(s) 430, 808, 914, 958, 1262
AfiI CCNNNNNNNGG 5 cut(s) 38, 43, 449, 884, 935
AgsI TTSAA 4 cut(s) 388, 798, 828, 1159
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 2 cut(s) 730, 877
AleI CACNNNNGTG 1 cut(s) 1187
AluBI AGCT 4 cut(s) 220, 551, 1016, 1155
AluI AGCT 4 cut(s) 220, 551, 1016, 1155
Alw21I GWGCWC 1 cut(s) 118
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 2 cut(s) 17, 488
Ama87I CYCGRG 2 cut(s) 288, 1320
AoxI GGCC 6 cut(s) 156, 535, 880, 998, 1191, 1307
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 3 cut(s) 162, 725, 1013
ApoI RAATTY 2 cut(s) 5, 667
AseI ATTAAT 1 cut(s) 1251
Asp718I GGTACC 1 cut(s) 912
AspLEI GCGC 5 cut(s) 167, 442, 455, 771, 1211
AspS9I GGNCC 4 cut(s) 392, 421, 875, 1308
AsuC2I CCSGG 2 cut(s) 12, 778
AsuHPI GGTGA 1 cut(s) 845
AsuNHI GCTAGC 2 cut(s) 89, 1016
AvaI CYCGRG 2 cut(s) 288, 1320
AvaII GGWCC 3 cut(s) 392, 421, 875
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 537
BanI GGYRCC 4 cut(s) 439, 452, 839, 912
BbrPI CACGTG 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 3 cut(s) 149, 712, 1025
BccI CCATC 4 cut(s) 137, 527, 746, 830
BceAI ACGGC 2 cut(s) 466, 1178
BcgI CGANNNNNNTGC 2 cut(s) 312, 346
BciT130I CCWGG 2 cut(s) 732, 879
BclI TGATCA 2 cut(s) 178, 1084
BcnI CCSGG 2 cut(s) 12, 778
BfaI CTAG 3 cut(s) 90, 213, 1017
BfmI CTRYAG 1 cut(s) 1011
BfoI RGCGCY 3 cut(s) 443, 456, 772
BglII AGATCT 1 cut(s) 209
BisI GCNGC 3 cut(s) 163, 726, 1014
BlsI GCNGC 3 cut(s) 164, 727, 1015
Bme1390I CCNGG 4 cut(s) 12, 732, 778, 879
Bme18I GGWCC 3 cut(s) 392, 421, 875
BmeT110I CYCGRG 2 cut(s) 288, 1320
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 4 cut(s) 392, 421, 875, 1308
BmiI GGNNCC 7 cut(s) 405, 441, 454, 526, 614, 841, 914
BmrFI CCNGG 4 cut(s) 12, 732, 778, 879
BmsI GCATC 2 cut(s) 304, 332
BmtI GCTAGC 2 cut(s) 93, 1020
BpiI GAAGAC 1 cut(s) 1170
BpuEI CTTGAG 1 cut(s) 1241
BpuMI CCSGG 2 cut(s) 12, 778
BsaAI YACGTR 2 cut(s) 477, 676
BsaHI GRCGYC 4 cut(s) 47, 440, 453, 1175
BsaJI CCNNGG 9 cut(s) 11, 37, 424, 538, 777, 883, 1026, 1100, 1212
BsaWI WCCGGW 1 cut(s) 1186
BsaXI ACNNNNNCTCC 2 cut(s) 726, 756
Bsc4I CCNNNNNNNGG 5 cut(s) 38, 43, 449, 884, 935
Bse118I RCCGGY 2 cut(s) 158, 227
Bse3DI GCAATG 1 cut(s) 1153
BseBI CCWGG 2 cut(s) 732, 879
BseDI CCNNGG 9 cut(s) 11, 37, 424, 538, 777, 883, 1026, 1100, 1212
BseGI GGATG 2 cut(s) 148, 649
BseLI CCNNNNNNNGG 5 cut(s) 38, 43, 449, 884, 935
BseMI GCAATG 1 cut(s) 1153
BseMII CTCAG 1 cut(s) 976
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 3 cut(s) 149, 712, 1025
BseYI CCCAGC 1 cut(s) 68
BsgI GTGCAG 1 cut(s) 711
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 6 cut(s) 158, 537, 882, 1000, 1193, 1309
BshNI GGYRCC 4 cut(s) 439, 452, 839, 912
BsiHKAI GWGCWC 1 cut(s) 118
BsiHKCI CYCGRG 2 cut(s) 288, 1320
BsiSI CCGG 5 cut(s) 11, 159, 228, 777, 1187
BslFI GGGAC 4 cut(s) 15, 373, 827, 1186
BslI CCNNNNNNNGG 5 cut(s) 38, 43, 449, 884, 935
BsmFI GGGAC 4 cut(s) 15, 373, 827, 1186
BsnI GGCC 6 cut(s) 158, 537, 882, 1000, 1193, 1309
BsoBI CYCGRG 2 cut(s) 288, 1320
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
Bsp19I CCATGG 3 cut(s) 37, 883, 1100
BspACI CCGC 5 cut(s) 93, 105, 111, 119, 140
BspANI GGCC 6 cut(s) 158, 537, 882, 1000, 1193, 1309
BspCNI CTCAG 1 cut(s) 975
BspFNI CGCG 1 cut(s) 121
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 7 cut(s) 405, 441, 454, 526, 614, 841, 914
BspMAI CTGCAG 1 cut(s) 1015
BspOI GCTAGC 2 cut(s) 93, 1020
BspPI GGATC 2 cut(s) 17, 488
BspT107I GGYRCC 4 cut(s) 439, 452, 839, 912
BsrDI GCAATG 1 cut(s) 1153
BsrFI RCCGGY 2 cut(s) 158, 227
BssAI RCCGGY 2 cut(s) 158, 227
BssECI CCNNGG 9 cut(s) 11, 37, 424, 538, 777, 883, 1026, 1100, 1212
BssMI GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
BssNI GRCGYC 4 cut(s) 47, 440, 453, 1175
BssT1I CCWWGG 6 cut(s) 37, 424, 538, 883, 1100, 1212
Bst2UI CCWGG 2 cut(s) 732, 879
Bst4CI ACNGT 5 cut(s) 601, 721, 956, 980, 1265
BstACI GRCGYC 4 cut(s) 47, 440, 453, 1175
BstBAI YACGTR 2 cut(s) 477, 676
BstC8I GCNNGC 7 cut(s) 91, 160, 235, 438, 695, 1018, 1207
BstDEI CTNAG 2 cut(s) 861, 962
BstDSI CCRYGG 3 cut(s) 37, 883, 1100
BstEII GGTNACC 1 cut(s) 1067
BstF5I GGATG 2 cut(s) 148, 649
BstFNI CGCG 1 cut(s) 121
BstH2I RGCGCY 3 cut(s) 443, 456, 772
BstHHI GCGC 5 cut(s) 167, 442, 455, 771, 1211
BstKTI GATC 6 cut(s) 25, 181, 212, 483, 1087, 1143
BstMBI GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
BstMWI GCNNNNNNNGC 4 cut(s) 164, 1022, 1152, 1215
BstNI CCWGG 2 cut(s) 732, 879
BstNSI RCATGY 2 cut(s) 321, 1209
BstPI GGTNACC 1 cut(s) 1067
BstSCI CCNGG 4 cut(s) 10, 730, 776, 877
BstSFI CTRYAG 1 cut(s) 1011
BstSLI GKGCMC 1 cut(s) 118
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 3 cut(s) 149, 712, 1025
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 2 cut(s) 22, 209
BstXI CCANNNNNNTGG 2 cut(s) 477, 1189
BstYI RGATCY 2 cut(s) 22, 209
BsuRI GGCC 6 cut(s) 158, 537, 882, 1000, 1193, 1309
BtgI CCRYGG 3 cut(s) 37, 883, 1100
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 2 cut(s) 148, 649
BtsI GCAGTG 2 cut(s) 249, 1020
BtsIMutI CAGTG 5 cut(s) 249, 717, 1020, 1115, 1270
Cac8I GCNNGC 7 cut(s) 91, 160, 235, 438, 695, 1018, 1207
CciI TCATGA 1 cut(s) 1053
CfoI GCGC 5 cut(s) 167, 442, 455, 771, 1211
Cfr10I RCCGGY 2 cut(s) 158, 227
Cfr13I GGNCC 4 cut(s) 392, 421, 875, 1308
CseI GACGC 2 cut(s) 36, 95
Csp6I GTAC 5 cut(s) 429, 807, 913, 957, 1261
CspCI CAANNNNNGTGG 2 cut(s) 1246, 1281
CviQI GTAC 5 cut(s) 429, 807, 913, 957, 1261
DdeI CTNAG 2 cut(s) 861, 962
DinI GGCGCC 2 cut(s) 441, 454
DpnI GATC 6 cut(s) 24, 180, 211, 482, 1086, 1142
DpnII GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
EaeI YGGCCR 3 cut(s) 156, 535, 1191
EciI GGCGGA 1 cut(s) 155
Eco130I CCWWGG 6 cut(s) 37, 424, 538, 883, 1100, 1212
Eco32I GATATC 1 cut(s) 1048
Eco47I GGWCC 3 cut(s) 392, 421, 875
Eco72I CACGTG 1 cut(s) 676
Eco88I CYCGRG 2 cut(s) 288, 1320
Eco91I GGTNACC 1 cut(s) 1067
EcoO109I RGGNCCY 1 cut(s) 392
EcoO65I GGTNACC 1 cut(s) 1067
EcoRII CCWGG 2 cut(s) 730, 877
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 6 cut(s) 37, 424, 538, 883, 1100, 1212
EcoT22I ATGCAT 2 cut(s) 307, 319
EgeI GGCGCC 2 cut(s) 441, 454
EheI GGCGCC 2 cut(s) 441, 454
ErhI CCWWGG 6 cut(s) 37, 424, 538, 883, 1100, 1212
FaqI GGGAC 4 cut(s) 15, 373, 827, 1186
FbaI TGATCA 2 cut(s) 178, 1084
Fnu4HI GCNGC 3 cut(s) 163, 726, 1014
FokI GGATG 2 cut(s) 155, 656
Fsp4HI GCNGC 3 cut(s) 163, 726, 1014
FspBI CTAG 3 cut(s) 90, 213, 1017
GlaI GCGC 5 cut(s) 166, 441, 454, 770, 1210
GluI GCNGC 3 cut(s) 163, 726, 1014
GsaI CCCAGC 1 cut(s) 72
HaeII RGCGCY 3 cut(s) 443, 456, 772
HaeIII GGCC 6 cut(s) 158, 537, 882, 1000, 1193, 1309
HapII CCGG 5 cut(s) 11, 159, 228, 777, 1187
HgaI GACGC 2 cut(s) 36, 95
HhaI GCGC 5 cut(s) 167, 442, 455, 771, 1211
Hin1I GRCGYC 4 cut(s) 47, 440, 453, 1175
Hin6I GCGC 5 cut(s) 165, 440, 453, 769, 1209
HinP1I GCGC 5 cut(s) 165, 440, 453, 769, 1209
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 7 cut(s) 28, 77, 794, 939, 987, 1105, 1323
HpaII CCGG 5 cut(s) 11, 159, 228, 777, 1187
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 3 cut(s) 116, 507, 875
Hpy188I TCNGA 3 cut(s) 27, 280, 1030
Hpy188III TCNNGA 7 cut(s) 625, 991, 1054, 1088, 1138, 1159, 1356
Hpy8I GTNNAC 3 cut(s) 116, 507, 875
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 10, 95, 903
HpyCH4III ACNGT 5 cut(s) 601, 721, 956, 980, 1265
HpyCH4IV ACGT 6 cut(s) 60, 243, 476, 675, 971, 1175
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 1022, 1152, 1215
HpyF3I CTNAG 2 cut(s) 861, 962
HpySE526I ACGT 6 cut(s) 60, 243, 476, 675, 971, 1175
Hsp92I GRCGYC 4 cut(s) 47, 440, 453, 1175
HspAI GCGC 5 cut(s) 165, 440, 453, 769, 1209
KasI GGCGCC 2 cut(s) 439, 452
KpnI GGTACC 1 cut(s) 916
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Ksp22I TGATCA 2 cut(s) 178, 1084
Kzo9I GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
LmnI GCTCC 3 cut(s) 172, 217, 409
Lsp1109I GCAGC 3 cut(s) 149, 712, 1025
LweI GCATC 2 cut(s) 304, 332
MaeI CTAG 3 cut(s) 90, 213, 1017
MaeII ACGT 6 cut(s) 60, 243, 476, 675, 971, 1175
MaeIII GTNAC 6 cut(s) 48, 244, 646, 865, 1067, 1226
MalI GATC 6 cut(s) 24, 180, 211, 482, 1086, 1142
MboI GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
MboII GAAGA 5 cut(s) 32, 110, 935, 1170, 1210
MfeI CAATTG 1 cut(s) 828
MflI RGATCY 2 cut(s) 22, 209
MhlI GDGCHC 1 cut(s) 118
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 6 cut(s) 5, 274, 667, 828, 1003, 1314
MluNI TGGCCA 1 cut(s) 537
Mly113I GGCGCC 2 cut(s) 440, 453
MlyI GAGTC 2 cut(s) 37, 1332
MmeI TCCRAC 3 cut(s) 63, 733, 999
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 2 cut(s) 307, 319
MroNI GCCGGC 1 cut(s) 158
MscI TGGCCA 1 cut(s) 537
MseI TTAA 4 cut(s) 603, 657, 744, 1251
MslI CAYNNNNRTG 4 cut(s) 134, 678, 1187, 1291
Msp20I TGGCCA 1 cut(s) 537
MspI CCGG 5 cut(s) 11, 159, 228, 777, 1187
MspR9I CCNGG 4 cut(s) 12, 732, 778, 879
MunI CAATTG 1 cut(s) 828
MvaI CCWGG 2 cut(s) 732, 879
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 4 cut(s) 164, 1022, 1152, 1215
NaeI GCCGGC 1 cut(s) 160
NarI GGCGCC 2 cut(s) 440, 453
NciI CCSGG 2 cut(s) 12, 778
NcoI CCATGG 3 cut(s) 37, 883, 1100
NdeII GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
NgoMIV GCCGGC 1 cut(s) 158
NheI GCTAGC 2 cut(s) 89, 1016
NlaIV GGNNCC 7 cut(s) 405, 441, 454, 526, 614, 841, 914
NmuCI GTSAC 3 cut(s) 48, 244, 1226
NsiI ATGCAT 2 cut(s) 307, 319
NspI RCATGY 2 cut(s) 321, 1209
OliI CACNNNNGTG 1 cut(s) 1187
PaeI GCATGC 1 cut(s) 1209
PaeR7I CTCGAG 1 cut(s) 1320
PagI TCATGA 1 cut(s) 1053
PdiI GCCGGC 1 cut(s) 160
PfeI GAWTC 5 cut(s) 77, 794, 939, 987, 1105
PflMI CCANNNNNTGG 2 cut(s) 43, 884
PkrI GCNGC 3 cut(s) 164, 727, 1015
PleI GAGTC 2 cut(s) 36, 1331
PluTI GGCGCC 2 cut(s) 443, 456
PmaCI CACGTG 1 cut(s) 676
PmlI CACGTG 1 cut(s) 676
PpsI GAGTC 2 cut(s) 36, 1331
Ppu21I YACGTR 2 cut(s) 477, 676
PpuMI RGGWCCY 1 cut(s) 392
PshBI ATTAAT 1 cut(s) 1251
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 730, 877
PspCI CACGTG 1 cut(s) 676
PspEI GGTNACC 1 cut(s) 1067
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 2 cut(s) 730, 877
PspN4I GGNNCC 7 cut(s) 405, 441, 454, 526, 614, 841, 914
PspPI GGNCC 4 cut(s) 392, 421, 875, 1308
PspPPI RGGWCCY 1 cut(s) 392
PspXI VCTCGAGB 1 cut(s) 1320
PstI CTGCAG 1 cut(s) 1015
PsuI RGATCY 2 cut(s) 22, 209
RsaI GTAC 5 cut(s) 430, 808, 914, 958, 1262
RsaNI GTAC 5 cut(s) 429, 807, 913, 957, 1261
RseI CAYNNNNRTG 4 cut(s) 134, 678, 1187, 1291
SaqAI TTAA 4 cut(s) 603, 657, 744, 1251
SatI GCNGC 3 cut(s) 163, 726, 1014
Sau3AI GATC 6 cut(s) 22, 178, 209, 480, 1084, 1140
Sau96I GGNCC 4 cut(s) 392, 421, 875, 1308
SchI GAGTC 2 cut(s) 37, 1332
ScrFI CCNGG 4 cut(s) 12, 732, 778, 879
SduI GDGCHC 1 cut(s) 118
SfaNI GCATC 2 cut(s) 304, 332
SfcI CTRYAG 1 cut(s) 1011
SfoI GGCGCC 2 cut(s) 441, 454
Sfr274I CTCGAG 1 cut(s) 1320
SinI GGWCC 3 cut(s) 392, 421, 875
SlaI CTCGAG 1 cut(s) 1320
SmiMI CAYNNNNRTG 4 cut(s) 134, 678, 1187, 1291
SmlI CTYRAG 2 cut(s) 1256, 1320
SmoI CTYRAG 2 cut(s) 1256, 1320
SphI GCATGC 1 cut(s) 1209
Sse9I AATT 6 cut(s) 5, 274, 667, 828, 1003, 1314
SsiI CCGC 5 cut(s) 93, 105, 111, 119, 140
SspDI GGCGCC 2 cut(s) 439, 452
SspI AATATT 1 cut(s) 787
SspMI CTAG 3 cut(s) 90, 213, 1017
StyD4I CCNGG 4 cut(s) 10, 730, 776, 877
StyI CCWWGG 6 cut(s) 37, 424, 538, 883, 1100, 1212
TaaI ACNGT 5 cut(s) 601, 721, 956, 980, 1265
TaiI ACGT 6 cut(s) 63, 246, 479, 678, 974, 1178
TaqI TCGA 3 cut(s) 464, 483, 1321
TasI AATT 6 cut(s) 5, 274, 667, 828, 1003, 1314
TatI WGTACW 1 cut(s) 1260
TfiI GAWTC 5 cut(s) 77, 794, 939, 987, 1105
Tru1I TTAA 4 cut(s) 603, 657, 744, 1251
Tru9I TTAA 4 cut(s) 603, 657, 744, 1251
TscAI CASTG 5 cut(s) 256, 724, 1027, 1115, 1270
TseFI GTSAC 3 cut(s) 48, 244, 1226
TseI GCWGC 3 cut(s) 162, 725, 1013
Tsp45I GTSAC 3 cut(s) 48, 244, 1226
TspDTI ATGAA 5 cut(s) 17, 480, 579, 1070, 1116
TspRI CASTG 5 cut(s) 256, 724, 1027, 1115, 1270
Van91I CCANNNNNTGG 2 cut(s) 43, 884
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 3 cut(s) 392, 421, 875
VspI ATTAAT 1 cut(s) 1251
XapI RAATTY 2 cut(s) 5, 667
XceI RCATGY 2 cut(s) 321, 1209
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XhoI CTCGAG 1 cut(s) 1320
XspI CTAG 3 cut(s) 90, 213, 1017
ZraI GACGTC 1 cut(s) 1176
Zsp2I ATGCAT 2 cut(s) 307, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.