pycom09g04500

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000434
Physical Location & Seq
Reverse (-)
3329941 .. 3331014
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g04500.2

Sequence Viewer

Length: 750 bp
ATGAGACATTATTCGCAGAATTTGAGGTTCAATTTTGTCACCAATTCTATAATTCGGGACATAACAACAAAGGACAGCAAAAATTTCCACGTCAATGTTTTGGGTTGCAACAACGTGGCATTCCAACATTTTACCGTCTCAGCGCCTAAAGAGAGCATGAACACAGATGGAATTCACATCGGACGTTCAACTGGGATCAACATCACAGATTCAATCATTGGAACTGGGGATGATTGCATCTCTATAGGTGATGGCACCAAGCAACTCCATGTAACCAAAGTTACTTATATCAGAATGAAGAACCTGTGTCCGGGATCGATCATATCCAATTTATTTATCAAGAACTGCACAATTAAAAATACAGACAATGGTGTGAGGATCAAAACATGGCCTGCTTCTCCATCAAATGGTGTAGTCTCAGATGTTCACTTTGAGGATATTATTATGGACAATGTTGGAAACCCTGTCATCATAGACCAAGAGTATTGTCCATGGAATCAGTGTACACTTAAGGTTCCATCCAAAGTTAAGATCAGCAATGTCAGCTTCAAAAACATAAAGGGCTCATCTTCATCCCCAGTCGGTATTAAGATTGTATGCAGTGGAGGGCTGCCATGTGAGAATGTGCAACTAAGTGACATTGATCTCACATACACTGGAAACAAAGGCTCTATTACCTCTCAATGTAAGCATGTCAAGCCCACCATTAACAACGTGGCACGGGCTCTTGCTTGTGCGACCTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

250

Amino Acids

27.06

Weight (kDa)

8.9

Isoelectric Point (pI)

29.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 407
AclWI GGATC 3 cut(s) 203, 322, 386
AcsI RAATTY 3 cut(s) 19, 82, 171
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 2 cut(s) 310, 407
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 4 cut(s) 31, 189, 213, 550
AjiI CACGTC 1 cut(s) 91
AluBI AGCT 1 cut(s) 546
AluI AGCT 1 cut(s) 546
Alw26I GTCTC 2 cut(s) 142, 421
AlwI GGATC 3 cut(s) 203, 322, 386
AoxI GGCC 1 cut(s) 389
ApeKI GCWGC 1 cut(s) 610
ApoI RAATTY 3 cut(s) 19, 82, 171
AspLEI GCGC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 2 cut(s) 31, 260
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 2 cut(s) 566, 727
BbvI GCAGC 1 cut(s) 597
BccI CCATC 4 cut(s) 161, 245, 409, 526
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 2 cut(s) 142, 421
BfmI CTRYAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 146
BfrI CTTAAG 1 cut(s) 509
BisI GCNGC 1 cut(s) 611
BlsI GCNGC 1 cut(s) 612
Bme1390I CCNGG 1 cut(s) 312
BmgBI CACGTC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 256, 516
BmrFI CCNGG 1 cut(s) 312
BmrI ACTGGG 3 cut(s) 201, 234, 572
BmsI GCATC 1 cut(s) 246
BmuI ACTGGG 3 cut(s) 201, 234, 572
BpuMI CCSGG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 491
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 407
Bse1I ACTGG 4 cut(s) 196, 229, 578, 661
Bse3DI GCAATG 1 cut(s) 544
Bse8I GATNNNNATC 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 317
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 3 cut(s) 235, 518, 572
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 2 cut(s) 310, 407
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 2 cut(s) 153, 432
BseNI ACTGG 4 cut(s) 196, 229, 578, 661
BseXI GCAGC 1 cut(s) 597
BsgI GTGCAG 1 cut(s) 331
BshFI GGCC 1 cut(s) 391
BshNI GGYRCC 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 317
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 310, 407
BsmAI GTCTC 2 cut(s) 142, 421
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 119
BsnI GGCC 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 566, 727
Bsp1407I TGTACA 1 cut(s) 503
Bsp143I GATC 6 cut(s) 195, 314, 318, 378, 531, 643
Bsp19I CCATGG 1 cut(s) 491
BspANI GGCC 1 cut(s) 391
BspCNI CTCAG 2 cut(s) 152, 431
BspDI ATCGAT 1 cut(s) 317
BspLI GGNNCC 2 cut(s) 256, 516
BspPI GGATC 3 cut(s) 203, 322, 386
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 1 cut(s) 509
BsrDI GCAATG 1 cut(s) 544
BsrGI TGTACA 1 cut(s) 503
BsrI ACTGG 4 cut(s) 196, 229, 578, 661
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 6 cut(s) 195, 314, 318, 378, 531, 643
BssT1I CCWWGG 1 cut(s) 491
Bst4CI ACNGT 1 cut(s) 136
BstAFI CTTAAG 1 cut(s) 509
BstAUI TGTACA 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 393
BstDEI CTNAG 3 cut(s) 139, 418, 632
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 3 cut(s) 235, 518, 572
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 6 cut(s) 198, 317, 321, 381, 534, 646
BstMAI GTCTC 2 cut(s) 142, 421
BstMBI GATC 6 cut(s) 195, 314, 318, 378, 531, 643
BstMWI GCNNNNNNNGC 2 cut(s) 543, 697
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 317
BsuRI GGCC 1 cut(s) 391
BsuTUI ATCGAT 1 cut(s) 317
BtgI CCRYGG 1 cut(s) 491
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 3 cut(s) 235, 518, 572
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 3 cut(s) 506, 607, 654
Cac8I GCNNGC 1 cut(s) 393
CfoI GCGC 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 317
Csp6I GTAC 1 cut(s) 504
CviAII CATG 6 cut(s) 157, 269, 387, 492, 615, 692
CviJI RGCY 7 cut(s) 391, 546, 564, 610, 669, 700, 725
CviKI_1 RGCY 7 cut(s) 391, 546, 564, 610, 669, 700, 725
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 3 cut(s) 139, 418, 632
DpnI GATC 6 cut(s) 197, 316, 320, 380, 533, 645
DpnII GATC 6 cut(s) 195, 314, 318, 378, 531, 643
Eco130I CCWWGG 1 cut(s) 491
Eco24I GRGCYC 2 cut(s) 566, 727
EcoRI GAATTC 1 cut(s) 171
EcoT14I CCWWGG 1 cut(s) 491
EcoT38I GRGCYC 2 cut(s) 566, 727
ErhI CCWWGG 1 cut(s) 491
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 6 cut(s) 160, 272, 390, 495, 618, 695
FaqI GGGAC 1 cut(s) 71
FatI CATG 6 cut(s) 156, 268, 386, 491, 614, 691
Fnu4HI GCNGC 1 cut(s) 611
FokI GGATG 3 cut(s) 242, 505, 559
FriOI GRGCYC 2 cut(s) 566, 727
Fsp4HI GCNGC 1 cut(s) 611
GlaI GCGC 1 cut(s) 144
GluI GCNGC 1 cut(s) 611
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 1 cut(s) 391
HapII CCGG 1 cut(s) 311
HhaI GCGC 1 cut(s) 145
Hin1II CATG 6 cut(s) 160, 272, 390, 495, 618, 695
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HinfI GANTC 2 cut(s) 209, 496
HpaII CCGG 1 cut(s) 311
HphI GGTGA 2 cut(s) 31, 260
Hpy166II GTNNAC 3 cut(s) 427, 504, 506
Hpy188I TCNGA 3 cut(s) 182, 293, 421
Hpy188III TCNNGA 3 cut(s) 56, 340, 747
Hpy8I GTNNAC 3 cut(s) 427, 504, 506
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 4 cut(s) 90, 114, 184, 714
HpyCH4V TGCA 5 cut(s) 108, 237, 348, 600, 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 543, 697
HpyF3I CTNAG 3 cut(s) 139, 418, 632
HpySE526I ACGT 4 cut(s) 90, 114, 184, 714
Hsp92II CATG 6 cut(s) 160, 272, 390, 495, 618, 695
HspAI GCGC 1 cut(s) 143
Kzo9I GATC 6 cut(s) 195, 314, 318, 378, 531, 643
LpnPI CCDG 8 cut(s) 177, 210, 317, 324, 405, 477, 591, 642
Lsp1109I GCAGC 1 cut(s) 597
LweI GCATC 1 cut(s) 246
MaeII ACGT 4 cut(s) 90, 114, 184, 714
MaeIII GTNAC 4 cut(s) 37, 271, 280, 635
MalI GATC 6 cut(s) 197, 316, 320, 380, 533, 645
MboI GATC 6 cut(s) 195, 314, 318, 378, 531, 643
MboII GAAGA 3 cut(s) 310, 561, 735
MhlI GDGCHC 2 cut(s) 566, 727
MluCI AATT 8 cut(s) 19, 31, 43, 51, 82, 171, 328, 351
MmeI TCCRAC 2 cut(s) 148, 436
MnlI CCTC 5 cut(s) 18, 369, 427, 599, 688
MseI TTAA 5 cut(s) 354, 510, 528, 588, 708
MslI CAYNNNNRTG 1 cut(s) 93
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 119
MwoI GCNNNNNNNGC 2 cut(s) 543, 697
NciI CCSGG 1 cut(s) 312
NcoI CCATGG 1 cut(s) 491
NdeII GATC 6 cut(s) 195, 314, 318, 378, 531, 643
NlaIII CATG 6 cut(s) 160, 272, 390, 495, 618, 695
NlaIV GGNNCC 2 cut(s) 256, 516
NmuCI GTSAC 2 cut(s) 37, 635
NspI RCATGY 1 cut(s) 695
PctI GAATGC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 209, 496
PflMI CCANNNNNTGG 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 1 cut(s) 612
PspN4I GGNNCC 2 cut(s) 256, 516
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 5 cut(s) 354, 510, 528, 588, 708
SatI GCNGC 1 cut(s) 611
Sau3AI GATC 6 cut(s) 195, 314, 318, 378, 531, 643
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 2 cut(s) 566, 727
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 8 cut(s) 19, 31, 43, 51, 82, 171, 328, 351
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 491
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 4 cut(s) 93, 117, 187, 717
TaqI TCGA 1 cut(s) 317
TasI AATT 8 cut(s) 19, 31, 43, 51, 82, 171, 328, 351
TatI WGTACW 1 cut(s) 503
TfiI GAWTC 2 cut(s) 209, 496
Tru1I TTAA 5 cut(s) 354, 510, 528, 588, 708
Tru9I TTAA 5 cut(s) 354, 510, 528, 588, 708
TscAI CASTG 3 cut(s) 506, 607, 661
TseFI GTSAC 2 cut(s) 37, 635
TseI GCWGC 1 cut(s) 610
Tsp45I GTSAC 2 cut(s) 37, 635
TspDTI ATGAA 3 cut(s) 173, 311, 561
TspRI CASTG 3 cut(s) 506, 607, 661
Van91I CCANNNNNTGG 1 cut(s) 407
Vha464I CTTAAG 1 cut(s) 509
XapI RAATTY 3 cut(s) 19, 82, 171
XceI RCATGY 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.