MD12G1142000.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000434
Physical Location & Seq
Forward (+)
22028744 .. 22030221
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1142000.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGACCTTTCGTTTCCAAATTCGATATGAAAGTTATATACTTGTTCCCAATTTTTTGTTTTCCGTTGGCTATTGGGCTTTTGTGTTCTTATTCTTAGCATCTATTGCTAATGCGAAAGCCGAAGTCTTTTATGTGACAAATTCAACATATGGAGGAAACCCTAGCGGCGATATTACGCTTGCTTTGGCTGACGCTTGGAGAGCTGCCTGTTCATCGCCATGGCCAGCCAAAGTTGTTATTCCAGAAGGGGAATACTACTTAAGAGGAGCAATTTTACATGGTCCATGCAAATCTCCCATTGAGGTTCAAGTTCAAGGGTATTTGCGTGCTCCACAAGAAAGCAGCCAACTCCTTCAACAGAATACTTGGGTTGGCTTTCAATACGTTGACAGGCTCACCTTATTTGGTGGTGGAACTTTTGATGGCCAAAGTAATTGCCACAAATATAAAAGTTGCCAATCCAACAGAGTATTTAATTTGAGGTTTGATTTTGTCACAAATTCAGTGATCGAGGGCATAACTTCACTAGATAGCAAAAATTTCCACATCAATGTTTTTGCATGTCGCAATGTTACATTCCAATATGTTACTATCACCGCACCTGAAGACAGTGTCAACACAGATGGAATTCACATCGGGAGATCGTTTGGGGTCACCATTGATCACACAATAATTGGAACAGGCGACGACTGCATTTCTCTTGGTGACGGTAGCCAGAACATTGTCGTGACCAACGTTACTTGTGGACCAGGGCATGGCATAAGCATCGGGAGCCTAGGAAAGTATCCAGACGAAGAGCCCGTGGTTGGAGTCAGAGTGAAGAACTGCACACTCACTAACACACAAAATGGTGTGAGAATCAAAACATGGCCGGGTTCTCCAACATTTACAACTGCCTCGCATATTCACTTTCAAGATATTAACATGGTAAATGTTAGTAACCCTATTTCTATAGACCAAGAGTACTGCCCTTACACTTATTGTGGAAAAGATGATCCATCCAAAGTCAAGATAAGCGACATCAGTTTCACCAACATCAAGGGCTCATCTGCAACCCCAGTTGCAGTCAAGCTTATGTGCAGTAGAAAAATACCGTGTGATGGAGTCGGGTTGACTGACGTGGACCTCACGTACATCGGAAGCAAAGGCCCTCTCACCTCTCAATGTGCTTATGTCAAGCCCTACATTACTCGCGTGGCGAATGCTCTTGCTTGTGCTACCTGGTCATATTCTAATAGTCTAAGAAGTGGAAGAAATCTCACTAATCTTCTCACTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

427

Amino Acids

46.66

Weight (kDa)

7.87

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 55 - 256 2.1e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 70 - 393 2.1e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 755
AccII CGCG 1 cut(s) 1194
AciI CCGC 2 cut(s) 165, 597
AclI AACGTT 1 cut(s) 735
AclWI GGATC 1 cut(s) 989
AcoI YGGCCR 3 cut(s) 221, 424, 869
AcsI RAATTY 5 cut(s) 18, 139, 499, 538, 627
AcuI CTGAAG 1 cut(s) 624
AfaI GTAC 2 cut(s) 965, 1133
AfiI CCNNNNNNNGG 3 cut(s) 755, 806, 1100
AflII CTTAAG 1 cut(s) 259
AgsI TTSAA 6 cut(s) 144, 308, 314, 356, 380, 914
AjiI CACGTC 1 cut(s) 1120
AjnI CCWGG 2 cut(s) 748, 1220
AluBI AGCT 2 cut(s) 203, 1072
AluI AGCT 2 cut(s) 203, 1072
Alw21I GWGCWC 1 cut(s) 331
AlwI GGATC 1 cut(s) 989
AoxI GGCC 4 cut(s) 221, 424, 869, 1147
ApeKI GCWGC 2 cut(s) 203, 342
ApoI RAATTY 5 cut(s) 18, 139, 499, 538, 627
AspA2I CCTAGG 1 cut(s) 775
AspS9I GGNCC 4 cut(s) 281, 746, 1123, 1148
AsuC2I CCSGG 1 cut(s) 873
AsuHPI GGTGA 6 cut(s) 388, 586, 646, 716, 1021, 1147
AvaII GGWCC 3 cut(s) 281, 746, 1123
AvrII CCTAGG 1 cut(s) 775
BalI TGGCCA 2 cut(s) 223, 426
BanII GRGCYC 2 cut(s) 801, 1046
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 2 cut(s) 190, 354
BccI CCATC 4 cut(s) 416, 617, 1006, 1094
BcgI CGANNNNNNTGC 2 cut(s) 748, 782
BciT130I CCWGG 2 cut(s) 750, 1222
BciVI GTATCC 1 cut(s) 795
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 1 cut(s) 873
BfaI CTAG 4 cut(s) 162, 527, 776, 1275
BfmI CTRYAG 1 cut(s) 951
BfrI CTTAAG 1 cut(s) 259
BfuI GTATCC 1 cut(s) 795
BisI GCNGC 3 cut(s) 166, 204, 343
BlnI CCTAGG 1 cut(s) 775
BlsI GCNGC 3 cut(s) 167, 205, 344
BmcAI AGTACT 1 cut(s) 965
Bme1390I CCNGG 3 cut(s) 750, 873, 1222
Bme18I GGWCC 3 cut(s) 281, 746, 1123
BmgBI CACGTC 1 cut(s) 1120
BmgT120I GGNCC 4 cut(s) 281, 746, 1123, 1148
BmiI GGNNCC 1 cut(s) 773
BmrFI CCNGG 3 cut(s) 750, 873, 1222
BmrI ACTGGG 1 cut(s) 1052
BmsI GCATC 2 cut(s) 107, 774
BmuI ACTGGG 1 cut(s) 1052
BpiI GAAGAC 1 cut(s) 612
BpuMI CCSGG 1 cut(s) 873
BsaAI YACGTR 1 cut(s) 1131
BsaJI CCNNGG 4 cut(s) 218, 749, 775, 801
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 755, 806, 1100
Bse1I ACTGG 1 cut(s) 1058
Bse3DI GCAATG 1 cut(s) 574
BseBI CCWGG 2 cut(s) 750, 1222
BseDI CCNNGG 4 cut(s) 218, 749, 775, 801
BseGI GGATG 1 cut(s) 998
BseLI CCNNNNNNNGG 3 cut(s) 755, 806, 1100
BseMI GCAATG 1 cut(s) 574
BseNI ACTGG 1 cut(s) 1058
BseRI GAGGAG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 190, 354
BsgI GTGCAG 2 cut(s) 811, 1099
Bsh1236I CGCG 1 cut(s) 1194
BshFI GGCC 4 cut(s) 223, 426, 871, 1149
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 1 cut(s) 872
BslI CCNNNNNNNGG 3 cut(s) 755, 806, 1100
BsmI GAATGC 1 cut(s) 1207
BsnI GGCC 4 cut(s) 223, 426, 871, 1149
Bsp1286I GDGCHC 3 cut(s) 331, 801, 1046
Bsp143I GATC 4 cut(s) 507, 641, 661, 994
Bsp19I CCATGG 1 cut(s) 218
BspACI CCGC 2 cut(s) 165, 597
BspANI GGCC 4 cut(s) 223, 426, 871, 1149
BspFNI CGCG 1 cut(s) 1194
BspLI GGNNCC 1 cut(s) 773
BspPI GGATC 1 cut(s) 989
BspQI GCTCTTC 1 cut(s) 789
BspTI CTTAAG 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 574
BsrI ACTGG 1 cut(s) 1058
BssECI CCNNGG 4 cut(s) 218, 749, 775, 801
BssMI GATC 4 cut(s) 507, 641, 661, 994
BssT1I CCWWGG 2 cut(s) 218, 775
Bst2UI CCWGG 2 cut(s) 750, 1222
Bst4CI ACNGT 3 cut(s) 611, 710, 1095
Bst6I CTCTTC 1 cut(s) 789
BstAFI CTTAAG 1 cut(s) 259
BstAPI GCANNNNNTGC 1 cut(s) 104
BstBAI YACGTR 1 cut(s) 1131
BstC8I GCNNGC 3 cut(s) 180, 225, 327
BstDEI CTNAG 2 cut(s) 94, 1241
BstDSI CCRYGG 2 cut(s) 218, 801
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 1 cut(s) 998
BstFNI CGCG 1 cut(s) 1194
BstKTI GATC 4 cut(s) 510, 644, 664, 997
BstMBI GATC 4 cut(s) 507, 641, 661, 994
BstMWI GCNNNNNNNGC 4 cut(s) 104, 200, 690, 771
BstNI CCWGG 2 cut(s) 750, 1222
BstNSI RCATGY 1 cut(s) 564
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 3 cut(s) 748, 871, 1220
BstSFI CTRYAG 1 cut(s) 951
BstUI CGCG 1 cut(s) 1194
BstV1I GCAGC 2 cut(s) 190, 354
BstV2I GAAGAC 1 cut(s) 612
BsuI GTATCC 1 cut(s) 795
BsuRI GGCC 4 cut(s) 223, 426, 871, 1149
BtgI CCRYGG 2 cut(s) 218, 801
BtgZI GCGATG 1 cut(s) 198
BtrI CACGTC 1 cut(s) 1120
BtsCI GGATG 1 cut(s) 998
BtsIMutI CAGTG 2 cut(s) 510, 616
Cac8I GCNNGC 3 cut(s) 180, 225, 327
Cfr13I GGNCC 4 cut(s) 281, 746, 1123, 1148
CseI GACGC 1 cut(s) 200
CsiI ACCWGGT 1 cut(s) 1220
Csp6I GTAC 2 cut(s) 964, 1132
CviAII CATG 7 cut(s) 219, 278, 285, 561, 755, 867, 925
CviQI GTAC 2 cut(s) 964, 1132
DdeI CTNAG 2 cut(s) 94, 1241
DpnI GATC 4 cut(s) 509, 643, 663, 996
DpnII GATC 4 cut(s) 507, 641, 661, 994
EaeI YGGCCR 3 cut(s) 221, 424, 869
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
Eco130I CCWWGG 2 cut(s) 218, 775
Eco24I GRGCYC 2 cut(s) 801, 1046
Eco47I GGWCC 3 cut(s) 281, 746, 1123
Eco57I CTGAAG 1 cut(s) 624
Eco91I GGTNACC 1 cut(s) 652
EcoO109I RGGNCCY 1 cut(s) 1148
EcoO65I GGTNACC 1 cut(s) 652
EcoRI GAATTC 1 cut(s) 627
EcoRII CCWGG 2 cut(s) 748, 1220
EcoT14I CCWWGG 2 cut(s) 218, 775
EcoT38I GRGCYC 2 cut(s) 801, 1046
ErhI CCWWGG 2 cut(s) 218, 775
FaeI CATG 7 cut(s) 222, 281, 288, 564, 758, 870, 928
FatI CATG 7 cut(s) 218, 277, 284, 560, 754, 866, 924
FauNDI CATATG 1 cut(s) 148
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 3 cut(s) 166, 204, 343
FokI GGATG 1 cut(s) 985
FriOI GRGCYC 2 cut(s) 801, 1046
Fsp4HI GCNGC 3 cut(s) 166, 204, 343
FspBI CTAG 4 cut(s) 162, 527, 776, 1275
GluI GCNGC 3 cut(s) 166, 204, 343
HaeIII GGCC 4 cut(s) 223, 426, 871, 1149
HapII CCGG 1 cut(s) 872
HgaI GACGC 1 cut(s) 200
Hin1II CATG 7 cut(s) 222, 281, 288, 564, 758, 870, 928
HincII GTYRAC 3 cut(s) 388, 616, 1113
HindII GTYRAC 3 cut(s) 388, 616, 1113
HindIII AAGCTT 1 cut(s) 1070
HinfI GANTC 3 cut(s) 810, 858, 1104
HpaII CCGG 1 cut(s) 872
HphI GGTGA 6 cut(s) 388, 586, 646, 716, 1021, 1147
Hpy166II GTNNAC 5 cut(s) 388, 616, 746, 1113, 1123
Hpy188I TCNGA 2 cut(s) 815, 1139
Hpy188III TCNNGA 7 cut(s) 242, 637, 727, 769, 788, 914, 1009
Hpy8I GTNNAC 5 cut(s) 388, 616, 746, 1113, 1123
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 2 cut(s) 239, 362
HpyCH4III ACNGT 3 cut(s) 611, 710, 1095
HpyCH4IV ACGT 4 cut(s) 384, 735, 1119, 1130
HpyCH4V TGCA 7 cut(s) 288, 560, 693, 828, 1052, 1064, 1080
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 200, 690, 771
HpyF3I CTNAG 2 cut(s) 94, 1241
HpySE526I ACGT 4 cut(s) 384, 735, 1119, 1130
Hsp92II CATG 7 cut(s) 222, 281, 288, 564, 758, 870, 928
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 4 cut(s) 507, 641, 661, 994
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 3 cut(s) 266, 334, 771
Lsp1109I GCAGC 2 cut(s) 190, 354
LweI GCATC 2 cut(s) 107, 774
MabI ACCWGGT 1 cut(s) 1220
MaeI CTAG 4 cut(s) 162, 527, 776, 1275
MaeII ACGT 4 cut(s) 384, 735, 1119, 1130
MaeIII GTNAC 9 cut(s) 133, 493, 571, 586, 652, 704, 727, 736, 938
MalI GATC 4 cut(s) 509, 643, 663, 996
MboI GATC 4 cut(s) 507, 641, 661, 994
MboII GAAGA 5 cut(s) 617, 806, 832, 1259, 1263
MhlI GDGCHC 3 cut(s) 331, 801, 1046
MlsI TGGCCA 2 cut(s) 223, 426
MluNI TGGCCA 2 cut(s) 223, 426
MlyI GAGTC 2 cut(s) 819, 1113
MmeI TCCRAC 3 cut(s) 486, 787, 905
MnlI CCTC 9 cut(s) 146, 257, 295, 474, 505, 907, 1136, 1161, 1168
Mox20I TGGCCA 2 cut(s) 223, 426
MscI TGGCCA 2 cut(s) 223, 426
MseI TTAA 3 cut(s) 260, 474, 921
MslI CAYNNNNRTG 3 cut(s) 217, 549, 725
Msp20I TGGCCA 2 cut(s) 223, 426
MspCI CTTAAG 1 cut(s) 259
MspI CCGG 1 cut(s) 872
MspR9I CCNGG 3 cut(s) 750, 873, 1222
Mva1269I GAATGC 1 cut(s) 1207
MvaI CCWGG 2 cut(s) 750, 1222
MvnI CGCG 1 cut(s) 1194
MwoI GCNNNNNNNGC 4 cut(s) 104, 200, 690, 771
NciI CCSGG 1 cut(s) 873
NcoI CCATGG 1 cut(s) 218
NdeI CATATG 1 cut(s) 148
NdeII GATC 4 cut(s) 507, 641, 661, 994
NlaIII CATG 7 cut(s) 222, 281, 288, 564, 758, 870, 928
NlaIV GGNNCC 1 cut(s) 773
NmuCI GTSAC 5 cut(s) 133, 493, 652, 704, 727
NspI RCATGY 1 cut(s) 564
PciSI GCTCTTC 1 cut(s) 789
PcsI WCGNNNNNNNCGW 2 cut(s) 732, 798
PctI GAATGC 1 cut(s) 1207
PfeI GAWTC 1 cut(s) 858
PflFI GACNNNGTC 1 cut(s) 611
PflMI CCANNNNNTGG 1 cut(s) 755
PkrI GCNGC 3 cut(s) 167, 205, 344
PleI GAGTC 2 cut(s) 818, 1112
PpsI GAGTC 2 cut(s) 818, 1112
Ppu21I YACGTR 1 cut(s) 1131
Psp1406I AACGTT 1 cut(s) 735
Psp6I CCWGG 2 cut(s) 748, 1220
PspEI GGTNACC 1 cut(s) 652
PspGI CCWGG 2 cut(s) 748, 1220
PspN4I GGNNCC 1 cut(s) 773
PspPI GGNCC 4 cut(s) 281, 746, 1123, 1148
PsyI GACNNNGTC 1 cut(s) 611
RsaI GTAC 2 cut(s) 965, 1133
RsaNI GTAC 2 cut(s) 964, 1132
RseI CAYNNNNRTG 3 cut(s) 217, 549, 725
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 3 cut(s) 260, 474, 921
SatI GCNGC 3 cut(s) 166, 204, 343
Sau3AI GATC 4 cut(s) 507, 641, 661, 994
Sau96I GGNCC 4 cut(s) 281, 746, 1123, 1148
ScaI AGTACT 1 cut(s) 965
SchI GAGTC 2 cut(s) 819, 1113
ScrFI CCNGG 3 cut(s) 750, 873, 1222
SduI GDGCHC 3 cut(s) 331, 801, 1046
SexAI ACCWGGT 1 cut(s) 1220
SfaNI GCATC 2 cut(s) 107, 774
SfcI CTRYAG 1 cut(s) 951
SinI GGWCC 3 cut(s) 281, 746, 1123
SmiMI CAYNNNNRTG 3 cut(s) 217, 549, 725
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
SsiI CCGC 2 cut(s) 165, 597
SspMI CTAG 4 cut(s) 162, 527, 776, 1275
StyD4I CCNGG 3 cut(s) 748, 871, 1220
StyI CCWWGG 2 cut(s) 218, 775
TaaI ACNGT 3 cut(s) 611, 710, 1095
TaiI ACGT 4 cut(s) 387, 738, 1122, 1133
TaqI TCGA 2 cut(s) 22, 510
TatI WGTACW 1 cut(s) 963
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 1 cut(s) 858
Tru1I TTAA 3 cut(s) 260, 474, 921
Tru9I TTAA 3 cut(s) 260, 474, 921
TscAI CASTG 2 cut(s) 510, 616
TseFI GTSAC 5 cut(s) 133, 493, 652, 704, 727
TseI GCWGC 2 cut(s) 203, 342
Tsp45I GTSAC 5 cut(s) 133, 493, 652, 704, 727
TspDTI ATGAA 2 cut(s) 42, 201
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 2 cut(s) 510, 616
Tth111I GACNNNGTC 1 cut(s) 611
Van91I CCANNNNNTGG 1 cut(s) 755
Vha464I CTTAAG 1 cut(s) 259
VpaK11BI GGWCC 3 cut(s) 281, 746, 1123
XapI RAATTY 5 cut(s) 18, 139, 499, 538, 627
XceI RCATGY 1 cut(s) 564
XmaJI CCTAGG 1 cut(s) 775
XspI CTAG 4 cut(s) 162, 527, 776, 1275
ZrmI AGTACT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.