Rh2AG522900

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000434
Physical Location & Seq
Forward (+)
74969089 .. 74970316
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG522900.1

Sequence Viewer

Length: 1005 bp
ATGGCTCTCAAACTTGTCATGGTTATGTTTTCATTACTTATGTTCTTATCCTTGACATCCTCGACAACTGCAAGGAGAGAGCTTCTAGCCGATGGTGGTGACTTTGACGTGACCAACGCGAAATATGGTGCAAAGCCCGATTCGGATATTACTGAGGCTTTAGCAAATGCTTGGAAAGATGCCTGTGCATCAACATCGGCAAGTAAAGTTATTGTTCCGAAAGGGACATTCAAAGTAAAAGAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCTATTGAGGTTCAGGTCCAAGGCACATTACAGGCGCCAGAAGACGCAAGCCAATTATCGAAACAAGATACTTGGATCGAGTTTTTATACCTCAACGACTTCACACTATCGGGTGGTGGAACCTTTGATGGCCAAGGACAAAAAACTTGGAAAGCCATTGATCCAAAAAACGCAAAGGCTGGCACCATTAATATCAGGTTCCACGCCGTCAAGAAGTCATTAGTTAAGGATATAACTTCACTTAATAGCAAGAACTTCCACATCAACGTAATAAGTTGCGAGCAACTTACGTTCGATCATGTCACCGTCACTGCACCTGGAGATAGCCCTAACACAGATGGAATACATATCGCACGCTCAACCGGGGTCAACGTTACTGATACAAATATCGGAACCGGAGATGATTGCATTTCAATTGGTGATGGTACCAAGCAACTTACAATAACTAAGGTTACTTGTGGACCAGGCCATGGAATAAGCGTTGGAAGTCTTGGAAGGTACGACAAAGAAGACCCTGTGGAAGGAATCAATGTTAAGAACTGTACCCTCAGTAAAACGACTAATGGTGTTAGAATCAAGACATGGGCTAAAAATCCTCAAGCTAGCACTTGCTCAGAAATTCACTTTGAGGATATCATCATGAATGATGTTCAAAACCCAATCGTCGTAGATCAAGAATACTGCCCATGGAATCAATGTGATAAACAGGTACTCTCCATACAACATGCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

334

Amino Acids

36.03

Weight (kDa)

5.99

Isoelectric Point (pI)

28.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 62 - 330 7.5e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 695
AccB1I GGYRCC 3 cut(s) 304, 452, 695
AccB7I CCANNNNNTGG 1 cut(s) 740
AccII CGCG 1 cut(s) 119
AclI AACGTT 1 cut(s) 642
AclWI GGATC 2 cut(s) 353, 425
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 1 cut(s) 888
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 4 cut(s) 697, 770, 814, 981
AfiI CCNNNNNNNGG 2 cut(s) 740, 791
AgsI TTSAA 4 cut(s) 232, 253, 684, 923
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 2 cut(s) 586, 733
AluBI AGCT 2 cut(s) 82, 872
AluI AGCT 2 cut(s) 82, 872
AlwI GGATC 2 cut(s) 353, 425
AoxI GGCC 2 cut(s) 400, 736
ApoI RAATTY 1 cut(s) 888
AseI ATTAAT 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 695
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 3 cut(s) 257, 286, 731
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 3 cut(s) 110, 565, 701
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 3 cut(s) 257, 286, 731
BalI TGGCCA 1 cut(s) 402
BanI GGYRCC 3 cut(s) 304, 452, 695
BbsI GAAGAC 2 cut(s) 318, 786
BccI CCATC 4 cut(s) 86, 392, 602, 686
BceAI ACGGC 1 cut(s) 461
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BciT130I CCWGG 2 cut(s) 588, 735
BcnI CCSGG 1 cut(s) 634
BfaI CTAG 2 cut(s) 86, 873
BfoI RGCGCY 1 cut(s) 308
Bme1390I CCNGG 3 cut(s) 588, 634, 735
Bme18I GGWCC 3 cut(s) 257, 286, 731
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 257, 286, 731
BmiI GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
BmrFI CCNGG 3 cut(s) 588, 634, 735
BmsI GCATC 2 cut(s) 169, 197
BmtI GCTAGC 1 cut(s) 876
BpiI GAAGAC 2 cut(s) 318, 786
BpmI CTGGAG 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 852
BpuMI CCSGG 1 cut(s) 634
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 5 cut(s) 289, 403, 633, 739, 956
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 2 cut(s) 740, 791
BseBI CCWGG 2 cut(s) 588, 735
BseDI CCNNGG 5 cut(s) 289, 403, 633, 739, 956
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 2 cut(s) 740, 791
BseMII CTCAG 3 cut(s) 144, 832, 897
BsgI GTGCAG 1 cut(s) 567
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 402, 738
BshNI GGYRCC 3 cut(s) 304, 452, 695
BsiSI CCGG 2 cut(s) 633, 666
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 2 cut(s) 740, 791
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 2 cut(s) 402, 738
Bsp143I GATC 4 cut(s) 345, 430, 565, 940
Bsp19I CCATGG 2 cut(s) 739, 956
BspANI GGCC 2 cut(s) 402, 738
BspCNI CTCAG 3 cut(s) 145, 831, 896
BspFNI CGCG 1 cut(s) 119
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 2 cut(s) 353, 425
BspT107I GGYRCC 3 cut(s) 304, 452, 695
BssECI CCNNGG 5 cut(s) 289, 403, 633, 739, 956
BssMI GATC 4 cut(s) 345, 430, 565, 940
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 4 cut(s) 289, 403, 739, 956
Bst2UI CCWGG 2 cut(s) 588, 735
Bst4CI ACNGT 2 cut(s) 577, 812
BstACI GRCGYC 1 cut(s) 305
BstC8I GCNNGC 5 cut(s) 319, 451, 551, 625, 874
BstDEI CTNAG 4 cut(s) 153, 717, 818, 883
BstDSI CCRYGG 2 cut(s) 739, 956
BstENI CCTNNNNNAGG 1 cut(s) 789
BstF5I GGATG 1 cut(s) 56
BstFNI CGCG 1 cut(s) 119
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 4 cut(s) 348, 433, 568, 943
BstMBI GATC 4 cut(s) 345, 430, 565, 940
BstNI CCWGG 2 cut(s) 588, 735
BstNSI RCATGY 1 cut(s) 998
BstSCI CCNGG 3 cut(s) 586, 632, 733
BstUI CGCG 1 cut(s) 119
BstV2I GAAGAC 2 cut(s) 318, 786
BsuRI GGCC 2 cut(s) 402, 738
BtgI CCRYGG 2 cut(s) 739, 956
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 56
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 5 cut(s) 319, 451, 551, 625, 874
CciI TCATGA 1 cut(s) 909
CfoI GCGC 1 cut(s) 307
Cfr13I GGNCC 3 cut(s) 257, 286, 731
CseI GACGC 1 cut(s) 323
Csp6I GTAC 4 cut(s) 696, 769, 813, 980
CviAII CATG 7 cut(s) 19, 569, 740, 852, 910, 957, 995
CviQI GTAC 4 cut(s) 696, 769, 813, 980
DdeI CTNAG 4 cut(s) 153, 717, 818, 883
DinI GGCGCC 1 cut(s) 306
DpnI GATC 4 cut(s) 347, 432, 567, 942
DpnII GATC 4 cut(s) 345, 430, 565, 940
EaeI YGGCCR 1 cut(s) 400
Eco130I CCWWGG 4 cut(s) 289, 403, 739, 956
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 3 cut(s) 257, 286, 731
EcoNI CCTNNNNNAGG 1 cut(s) 789
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 586, 733
EcoRV GATATC 1 cut(s) 904
EcoT14I CCWWGG 4 cut(s) 289, 403, 739, 956
EcoT22I ATGCAT 1 cut(s) 1000
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 4 cut(s) 289, 403, 739, 956
FaeI CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 998
FaqI GGGAC 1 cut(s) 238
FatI CATG 7 cut(s) 18, 568, 739, 851, 909, 956, 994
FokI GGATG 1 cut(s) 43
FspBI CTAG 2 cut(s) 86, 873
GlaI GCGC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 2 cut(s) 402, 738
HapII CCGG 2 cut(s) 633, 666
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 998
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HinfI GANTC 4 cut(s) 140, 795, 843, 961
HpaII CCGG 2 cut(s) 633, 666
HphI GGTGA 3 cut(s) 110, 565, 701
Hpy166II GTNNAC 2 cut(s) 640, 731
Hpy188I TCNGA 4 cut(s) 145, 219, 662, 886
Hpy188III TCNNGA 4 cut(s) 481, 847, 910, 944
Hpy8I GTNNAC 2 cut(s) 640, 731
Hpy99I CGWCG 1 cut(s) 938
HpyAV CCTTC 2 cut(s) 759, 785
HpyCH4III ACNGT 2 cut(s) 577, 812
HpyCH4IV ACGT 4 cut(s) 108, 537, 560, 642
HpyCH4V TGCA 7 cut(s) 71, 131, 188, 264, 584, 678, 998
HpyF3I CTNAG 4 cut(s) 153, 717, 818, 883
HpySE526I ACGT 4 cut(s) 108, 537, 560, 642
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 998
HspAI GCGC 1 cut(s) 305
KasI GGCGCC 1 cut(s) 304
KpnI GGTACC 1 cut(s) 699
Kzo9I GATC 4 cut(s) 345, 430, 565, 940
LmnI GCTCC 1 cut(s) 274
LweI GCATC 2 cut(s) 169, 197
MaeI CTAG 2 cut(s) 86, 873
MaeII ACGT 4 cut(s) 108, 537, 560, 642
MaeIII GTNAC 6 cut(s) 98, 109, 571, 577, 643, 721
MalI GATC 4 cut(s) 347, 432, 567, 942
MboI GATC 4 cut(s) 345, 430, 565, 940
MboII GAAGA 2 cut(s) 323, 791
MfeI CAATTG 1 cut(s) 684
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 3 cut(s) 323, 684, 888
MluNI TGGCCA 1 cut(s) 402
Mly113I GGCGCC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 733
MnlI CCTC 7 cut(s) 70, 148, 271, 371, 827, 876, 892
Mox20I TGGCCA 1 cut(s) 402
Mph1103I ATGCAT 1 cut(s) 1000
MscI TGGCCA 1 cut(s) 402
MseI TTAA 5 cut(s) 459, 495, 513, 804, 1003
MslI CAYNNNNRTG 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 402
MspI CCGG 2 cut(s) 633, 666
MspR9I CCNGG 3 cut(s) 588, 634, 735
MunI CAATTG 1 cut(s) 684
MvaI CCWGG 2 cut(s) 588, 735
MvnI CGCG 1 cut(s) 119
NarI GGCGCC 1 cut(s) 305
NciI CCSGG 1 cut(s) 634
NcoI CCATGG 2 cut(s) 739, 956
NdeII GATC 4 cut(s) 345, 430, 565, 940
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 998
NlaIV GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
NmuCI GTSAC 4 cut(s) 98, 109, 571, 577
NsiI ATGCAT 1 cut(s) 1000
NspI RCATGY 1 cut(s) 998
PagI TCATGA 1 cut(s) 909
PfeI GAWTC 4 cut(s) 140, 795, 843, 961
PflMI CCANNNNNTGG 1 cut(s) 740
PluTI GGCGCC 1 cut(s) 308
PpuMI RGGWCCY 1 cut(s) 257
PshBI ATTAAT 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 586, 733
PspGI CCWGG 2 cut(s) 586, 733
PspN4I GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
PspPI GGNCC 3 cut(s) 257, 286, 731
PspPPI RGGWCCY 1 cut(s) 257
RsaI GTAC 4 cut(s) 697, 770, 814, 981
RsaNI GTAC 4 cut(s) 696, 769, 813, 980
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 5 cut(s) 459, 495, 513, 804, 1003
Sau3AI GATC 4 cut(s) 345, 430, 565, 940
Sau96I GGNCC 3 cut(s) 257, 286, 731
ScrFI CCNGG 3 cut(s) 588, 634, 735
SfaNI GCATC 2 cut(s) 169, 197
SfoI GGCGCC 1 cut(s) 306
SinI GGWCC 3 cut(s) 257, 286, 731
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 867
SmoI CTYRAG 1 cut(s) 867
Sse9I AATT 3 cut(s) 323, 684, 888
SspDI GGCGCC 1 cut(s) 304
SspMI CTAG 2 cut(s) 86, 873
StyD4I CCNGG 3 cut(s) 586, 632, 733
StyI CCWWGG 4 cut(s) 289, 403, 739, 956
TaaI ACNGT 2 cut(s) 577, 812
TaiI ACGT 4 cut(s) 111, 540, 563, 645
TaqI TCGA 4 cut(s) 62, 329, 348, 564
TasI AATT 3 cut(s) 323, 684, 888
TfiI GAWTC 4 cut(s) 140, 795, 843, 961
Tru1I TTAA 5 cut(s) 459, 495, 513, 804, 1003
Tru9I TTAA 5 cut(s) 459, 495, 513, 804, 1003
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 4 cut(s) 98, 109, 571, 577
Tsp45I GTSAC 4 cut(s) 98, 109, 571, 577
TspDTI ATGAA 2 cut(s) 21, 926
TspRI CASTG 1 cut(s) 586
Van91I CCANNNNNTGG 1 cut(s) 740
VpaK11BI GGWCC 3 cut(s) 257, 286, 731
VspI ATTAAT 1 cut(s) 459
XagI CCTNNNNNAGG 1 cut(s) 789
XapI RAATTY 1 cut(s) 888
XceI RCATGY 1 cut(s) 998
XspI CTAG 2 cut(s) 86, 873
Zsp2I ATGCAT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.