MD00G1117800.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000434
Physical Location & Seq
Reverse (-)
25040787 .. 25041302
516 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1117800.v1.1.491

Sequence Viewer

Length: 516 bp
ATGAGGTTCAACTTCGTTACCAATTCCACAATTCAGGACATAACTTCCCTCGACATCAAGAATTTCCACGTCAACATTTTGGGGTGCCACAATGTCACATTCCAACGTGTCAATGTCACGGCACCTGGAGAAAGCATAAATACGGATGGAATCCATATCGAGCGTTCAACTGGAATCAACATTACTGATGCACACACTGGAACTGGAGAAGACTGTGTTTCGATTGGCAATGGAAACAAAGAAAGCACCATAACTAGGGTTACTTGTGGACCAGGCCATGGCATAAGCATTGGAAGTCTTGGAAGGTATTCGAATGAAGAACCTGTGGTTGGAAACAGAGTTACAAACCGCACCTTGACTAATACATCCAACGGTGTGAGAATCAAGACATGGTCTGCTTCTCCTTCGGATGGTATTGCAACAGATATGCATCTTGAAGATATTATCATGGTCAATGTTAACAATTCTGTCCTCATAGACCAACAGTACTCCCGTGGAAACAGTGTATTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

172

Amino Acids

18.5

Weight (kDa)

5.92

Isoelectric Point (pI)

27.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 1 - 167 1.9e-49 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 84, 121
AccB7I CCANNNNNTGG 1 cut(s) 278
AciI CCGC 1 cut(s) 349
AcsI RAATTY 1 cut(s) 61
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 4 cut(s) 255, 278, 329, 410
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 3 cut(s) 10, 168, 437
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 2 cut(s) 124, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AoxI GGCC 1 cut(s) 274
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 269
AsuII TTCGAA 1 cut(s) 311
AvaII GGWCC 1 cut(s) 269
BanI GGYRCC 2 cut(s) 84, 121
BbsI GAAGAC 1 cut(s) 216
BccI CCATC 2 cut(s) 140, 404
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 2 cut(s) 126, 273
BfaI CTAG 1 cut(s) 255
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 2 cut(s) 126, 273
Bme18I GGWCC 1 cut(s) 269
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 2 cut(s) 86, 123
BmrFI CCNGG 2 cut(s) 126, 273
BmsI GCATC 2 cut(s) 178, 439
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 2 cut(s) 147, 225
Bpu14I TTCGAA 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 277, 493
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 278, 329, 410
Bse1I ACTGG 3 cut(s) 175, 202, 208
Bse3DI GCAATG 1 cut(s) 235
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 2 cut(s) 126, 273
BseDI CCNNGG 2 cut(s) 277, 493
BseGI GGATG 3 cut(s) 151, 365, 415
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 4 cut(s) 255, 278, 329, 410
BseMI GCAATG 1 cut(s) 235
BseNI ACTGG 3 cut(s) 175, 202, 208
BshFI GGCC 1 cut(s) 276
BshNI GGYRCC 2 cut(s) 84, 121
BslI CCNNNNNNNGG 4 cut(s) 255, 278, 329, 410
BsnI GGCC 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 311
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 1 cut(s) 349
BspANI GGCC 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 86, 123
BspT104I TTCGAA 1 cut(s) 311
BspT107I GGYRCC 2 cut(s) 84, 121
BsrDI GCAATG 1 cut(s) 235
BsrI ACTGG 3 cut(s) 175, 202, 208
BssECI CCNNGG 2 cut(s) 277, 493
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 2 cut(s) 126, 273
Bst4CI ACNGT 4 cut(s) 215, 374, 486, 503
BstBI TTCGAA 1 cut(s) 311
BstDSI CCRYGG 2 cut(s) 277, 493
BstF5I GGATG 3 cut(s) 151, 365, 415
BstNI CCWGG 2 cut(s) 126, 273
BstSCI CCNGG 2 cut(s) 124, 271
BstV2I GAAGAC 1 cut(s) 216
BsuRI GGCC 1 cut(s) 276
BtgI CCRYGG 2 cut(s) 277, 493
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 3 cut(s) 151, 365, 415
BtsIMutI CAGTG 2 cut(s) 195, 508
Cfr13I GGNCC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 487
CviAII CATG 3 cut(s) 278, 390, 448
CviJI RGCY 1 cut(s) 276
CviKI_1 RGCY 1 cut(s) 276
CviQI GTAC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 269
EcoRII CCWGG 2 cut(s) 124, 271
EcoT14I CCWWGG 1 cut(s) 277
EcoT22I ATGCAT 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 3 cut(s) 281, 393, 451
FatI CATG 3 cut(s) 277, 389, 447
FokI GGATG 3 cut(s) 158, 352, 422
FspBI CTAG 1 cut(s) 255
GsuI CTGGAG 2 cut(s) 147, 225
HaeIII GGCC 1 cut(s) 276
Hin1II CATG 3 cut(s) 281, 393, 451
HincII GTYRAC 2 cut(s) 73, 460
HindII GTYRAC 2 cut(s) 73, 460
HinfI GANTC 3 cut(s) 150, 174, 381
HpaI GTTAAC 1 cut(s) 460
Hpy166II GTNNAC 3 cut(s) 73, 269, 460
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 4 cut(s) 35, 58, 385, 434
Hpy8I GTNNAC 3 cut(s) 73, 269, 460
HpyAV CCTTC 2 cut(s) 297, 414
HpyCH4III ACNGT 4 cut(s) 215, 374, 486, 503
HpyCH4IV ACGT 2 cut(s) 69, 106
HpyCH4V TGCA 3 cut(s) 191, 419, 430
HpySE526I ACGT 2 cut(s) 69, 106
Hsp92II CATG 3 cut(s) 281, 393, 451
KspAI GTTAAC 1 cut(s) 460
LpnPI CCDG 9 cut(s) 20, 111, 138, 156, 183, 189, 258, 285, 336
LweI GCATC 2 cut(s) 178, 439
MaeI CTAG 1 cut(s) 255
MaeII ACGT 2 cut(s) 69, 106
MaeIII GTNAC 5 cut(s) 16, 94, 115, 259, 340
MboII GAAGA 3 cut(s) 221, 329, 449
MluCI AATT 4 cut(s) 22, 30, 61, 463
MmeI TCCRAC 3 cut(s) 127, 310, 393
MnlI CCTC 2 cut(s) 59, 482
Mph1103I ATGCAT 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 307
MseI TTAA 1 cut(s) 459
MspR9I CCNGG 2 cut(s) 126, 273
MvaI CCWGG 2 cut(s) 126, 273
NcoI CCATGG 1 cut(s) 277
NlaIII CATG 3 cut(s) 281, 393, 451
NlaIV GGNNCC 2 cut(s) 86, 123
NmuCI GTSAC 2 cut(s) 94, 115
NsiI ATGCAT 1 cut(s) 432
NspV TTCGAA 1 cut(s) 311
PdmI GAANNNNTTC 1 cut(s) 307
PfeI GAWTC 3 cut(s) 150, 174, 381
PflFI GACNNNGTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 124, 271
PspGI CCWGG 2 cut(s) 124, 271
PspN4I GGNNCC 2 cut(s) 86, 123
PspPI GGNCC 1 cut(s) 269
PsyI GACNNNGTC 1 cut(s) 391
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
SaqAI TTAA 1 cut(s) 459
Sau96I GGNCC 1 cut(s) 269
ScaI AGTACT 1 cut(s) 488
ScrFI CCNGG 2 cut(s) 126, 273
SetI ASST 7 cut(s) 8, 72, 109, 127, 308, 325, 356
SfaNI GCATC 2 cut(s) 178, 439
SfuI TTCGAA 1 cut(s) 311
SinI GGWCC 1 cut(s) 269
Sse9I AATT 4 cut(s) 22, 30, 61, 463
SsiI CCGC 1 cut(s) 349
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 124, 271
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 4 cut(s) 215, 374, 486, 503
TaiI ACGT 2 cut(s) 72, 109
TaqI TCGA 4 cut(s) 51, 159, 221, 311
TasI AATT 4 cut(s) 22, 30, 61, 463
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 3 cut(s) 150, 174, 381
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 2 cut(s) 202, 508
TseFI GTSAC 2 cut(s) 94, 115
Tsp45I GTSAC 2 cut(s) 94, 115
TspDTI ATGAA 1 cut(s) 330
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 2 cut(s) 202, 508
Tth111I GACNNNGTC 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 307
XspI CTAG 1 cut(s) 255
ZrmI AGTACT 1 cut(s) 488
Zsp2I ATGCAT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.