MD09G1120100.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000434
Physical Location & Seq
Reverse (-)
9282233 .. 9284551
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1120100.v1.1.491

Sequence Viewer

Length: 867 bp
ATGCATGGGAAAAAAATGAATAAGCGGAAAATCATTTCCGTTCTAGAATTGGCTTTGAGCAAAGCTTGGACAGATGCATGTGCATCGACGTCGGCAAGTAAAATTGTTGTTCCAAGTGGGACATACAAGTTTGTAGGGGCAACTTTCAAAGGACCATGCAAAGCTGCTATTGAGTTTCAGCTAAAAGGCACAGTGCAGGCTCCAGAACATATCAAGGATCTCCCAAAACAAGATACTTGGGTAGCTTTTGAGCGCATTGATGGGCTCACCTTATCTGGTGGTGGAACTTTCGATGGCCAAGGAGCAACTGCTTGGAAGCAAAATGATTGTCACAAAAACAAAGATTGCAAATCTAAAGCTATCAGCGTCAATACAAAAGGAATTCATATGGGACGTTCATCTGGGATCAACATCAGAGATACAACCATTGGAACTGGGGATGATTGCATCTCTATAGGTCACGGAACCAACCAACTCCATGTAACCAACGTTAAATGCGGACCAGGCCACGGCATAAGCATCGGAAGCCTCGGAAGGACTGAGGATGAAGAACCTGTGTCCGGAATCTTTATCAAGAACTGCACGATTACAAATACAGATAATGGTGTTTCATCCAAAATTAAGATCAGTGATGTCAGCTTTAAGAACATAAAGGGCTCATCTTCAGGGATTTGGATTGTCTGCCCTCTAATTGTGGTGCCCTCCTCATGCCTTTCTGTTTTTGTGCTGCCATGTGAGAATGTGAAACCGAGTGACATTGATCTCACATACACTGGAGAGAAAGGCTCTATTACCTCTAAATGTAAACATGTTAAGCCCACCATTAACAACGTGGCAAAGCCTCTTGCTTGTGCTACATCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

289

Amino Acids

30.49

Weight (kDa)

9.08

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 27 - 121 3.1e-17 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 120 - 203 3.4e-28 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 697
AccIII TCCGGA 1 cut(s) 560
AciI CCGC 2 cut(s) 25, 498
AclI AACGTT 1 cut(s) 489
AclWI GGATC 2 cut(s) 225, 413
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 509, 560
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 1 cut(s) 502
AluBI AGCT 6 cut(s) 65, 164, 181, 245, 359, 639
AluI AGCT 6 cut(s) 65, 164, 181, 245, 359, 639
AlwI GGATC 2 cut(s) 225, 413
Aor13HI TCCGGA 1 cut(s) 560
AoxI GGCC 2 cut(s) 295, 505
ApeKI GCWGC 2 cut(s) 164, 727
ApoI RAATTY 1 cut(s) 381
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 152, 500
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 152, 500
BaeGI GKGCMC 1 cut(s) 702
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BalI TGGCCA 1 cut(s) 297
BanI GGYRCC 1 cut(s) 697
BanII GRGCYC 2 cut(s) 267, 659
BbvI GCAGC 2 cut(s) 151, 714
BccI CCATC 2 cut(s) 254, 287
BceAI ACGGC 1 cut(s) 526
BcgI CGANNNNNNTGC 6 cut(s) 66, 72, 100, 106, 502, 536
BciT130I CCWGG 1 cut(s) 504
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 453
BisI GCNGC 2 cut(s) 165, 728
BlsI GCNGC 2 cut(s) 166, 729
Bme1390I CCNGG 1 cut(s) 504
Bme18I GGWCC 2 cut(s) 152, 500
BmgT120I GGNCC 2 cut(s) 152, 500
BmiI GGNNCC 3 cut(s) 201, 466, 699
BmrFI CCNGG 1 cut(s) 504
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 4 cut(s) 64, 92, 456, 528
BmuI ACTGGG 1 cut(s) 444
BplI GAGNNNNNCTC 2 cut(s) 770, 802
BpmI CTGGAG 2 cut(s) 186, 795
BsaBI GATNNNNATC 1 cut(s) 410
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 298, 508, 529
BsaWI WCCGGW 1 cut(s) 560
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 560
Bse1I ACTGG 2 cut(s) 439, 778
Bse8I GATNNNNATC 1 cut(s) 410
BseAI TCCGGA 1 cut(s) 560
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 3 cut(s) 298, 508, 529
BseGI GGATG 3 cut(s) 445, 550, 611
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 2 cut(s) 509, 560
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 2 cut(s) 439, 778
BseRI GAGGAG 1 cut(s) 694
BseSI GKGCMC 1 cut(s) 702
BseXI GCAGC 2 cut(s) 151, 714
BsgI GTGCAG 2 cut(s) 215, 565
BshFI GGCC 2 cut(s) 297, 507
BshNI GGYRCC 1 cut(s) 697
BsiSI CCGG 1 cut(s) 561
BslFI GGGAC 2 cut(s) 133, 405
BslI CCNNNNNNNGG 2 cut(s) 509, 560
BsmFI GGGAC 2 cut(s) 133, 405
BsnI GGCC 2 cut(s) 297, 507
Bsp1286I GDGCHC 3 cut(s) 267, 659, 702
Bsp13I TCCGGA 1 cut(s) 560
Bsp143I GATC 4 cut(s) 217, 405, 624, 760
BspACI CCGC 2 cut(s) 25, 498
BspANI GGCC 2 cut(s) 297, 507
BspCNI CTCAG 1 cut(s) 532
BspEI TCCGGA 1 cut(s) 560
BspLI GGNNCC 3 cut(s) 201, 466, 699
BspPI GGATC 2 cut(s) 225, 413
BspT107I GGYRCC 1 cut(s) 697
BsrI ACTGG 2 cut(s) 439, 778
BssECI CCNNGG 3 cut(s) 298, 508, 529
BssMI GATC 4 cut(s) 217, 405, 624, 760
BssNI GRCGYC 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 1 cut(s) 193
BstACI GRCGYC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 1 cut(s) 540
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 3 cut(s) 445, 550, 611
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 4 cut(s) 220, 408, 627, 763
BstMBI GATC 4 cut(s) 217, 405, 624, 760
BstMWI GCNNNNNNNGC 2 cut(s) 504, 525
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 2 cut(s) 81, 812
BstSCI CCNGG 1 cut(s) 502
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 1 cut(s) 702
BstV1I GCAGC 2 cut(s) 151, 714
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 2 cut(s) 297, 507
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 3 cut(s) 445, 550, 611
BtsIMutI CAGTG 3 cut(s) 198, 634, 771
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 152, 500
CseI GACGC 1 cut(s) 355
CviAII CATG 7 cut(s) 5, 78, 156, 479, 708, 732, 809
DdeI CTNAG 1 cut(s) 540
DpnI GATC 4 cut(s) 219, 407, 626, 762
DpnII GATC 4 cut(s) 217, 405, 624, 760
EaeI YGGCCR 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 2 cut(s) 267, 659
Eco47I GGWCC 2 cut(s) 152, 500
Eco57I CTGAAG 1 cut(s) 648
EcoRI GAATTC 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 298
EcoT22I ATGCAT 2 cut(s) 6, 79
EcoT38I GRGCYC 2 cut(s) 267, 659
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 7 cut(s) 8, 81, 159, 482, 711, 735, 812
FaqI GGGAC 2 cut(s) 133, 405
FatI CATG 7 cut(s) 4, 77, 155, 478, 707, 731, 808
FauNDI CATATG 1 cut(s) 387
Fnu4HI GCNGC 2 cut(s) 165, 728
FokI GGATG 3 cut(s) 452, 557, 598
FriOI GRGCYC 2 cut(s) 267, 659
Fsp4HI GCNGC 2 cut(s) 165, 728
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 254
GluI GCNGC 2 cut(s) 165, 728
GsuI CTGGAG 2 cut(s) 186, 795
HaeIII GGCC 2 cut(s) 297, 507
HapII CCGG 1 cut(s) 561
HgaI GACGC 1 cut(s) 355
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 7 cut(s) 8, 81, 159, 482, 711, 735, 812
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 1 cut(s) 564
HpaII CCGG 1 cut(s) 561
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 1 cut(s) 806
Hpy188I TCNGA 3 cut(s) 416, 524, 533
Hpy188III TCNNGA 4 cut(s) 44, 203, 561, 574
Hpy8I GTNNAC 1 cut(s) 806
Hpy99I CGWCG 2 cut(s) 91, 94
HpyAV CCTTC 1 cut(s) 528
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 4 cut(s) 89, 394, 489, 831
HpyCH4V TGCA 8 cut(s) 4, 77, 83, 159, 196, 348, 447, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 504, 525
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 4 cut(s) 89, 394, 489, 831
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 7 cut(s) 8, 81, 159, 482, 711, 735, 812
HspAI GCGC 1 cut(s) 253
Kpn2I TCCGGA 1 cut(s) 560
Kzo9I GATC 4 cut(s) 217, 405, 624, 760
LmnI GCTCC 2 cut(s) 205, 302
Lsp1109I GCAGC 2 cut(s) 151, 714
LweI GCATC 4 cut(s) 64, 92, 456, 528
MaeI CTAG 1 cut(s) 44
MaeII ACGT 4 cut(s) 89, 394, 489, 831
MaeIII GTNAC 4 cut(s) 329, 458, 481, 752
MalI GATC 4 cut(s) 219, 407, 626, 762
MboI GATC 4 cut(s) 217, 405, 624, 760
MboII GAAGA 3 cut(s) 560, 654, 852
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 3 cut(s) 267, 659, 702
MlsI TGGCCA 1 cut(s) 297
MluCI AATT 5 cut(s) 47, 102, 381, 618, 690
MluNI TGGCCA 1 cut(s) 297
MnlI CCTC 7 cut(s) 535, 539, 696, 712, 715, 805, 852
Mox20I TGGCCA 1 cut(s) 297
Mph1103I ATGCAT 2 cut(s) 6, 79
MroI TCCGGA 1 cut(s) 560
MscI TGGCCA 1 cut(s) 297
MseI TTAA 6 cut(s) 492, 621, 642, 813, 825, 865
Msp20I TGGCCA 1 cut(s) 297
MspI CCGG 1 cut(s) 561
MspR9I CCNGG 1 cut(s) 504
MvaI CCWGG 1 cut(s) 504
MwoI GCNNNNNNNGC 2 cut(s) 504, 525
NdeI CATATG 1 cut(s) 387
NdeII GATC 4 cut(s) 217, 405, 624, 760
NlaIII CATG 7 cut(s) 8, 81, 159, 482, 711, 735, 812
NlaIV GGNNCC 3 cut(s) 201, 466, 699
NmuCI GTSAC 3 cut(s) 329, 458, 752
NsiI ATGCAT 2 cut(s) 6, 79
NspI RCATGY 2 cut(s) 81, 812
PciI ACATGT 1 cut(s) 808
PfeI GAWTC 1 cut(s) 564
PkrI GCNGC 2 cut(s) 166, 729
PscI ACATGT 1 cut(s) 808
Psp1406I AACGTT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 3 cut(s) 201, 466, 699
PspPI GGNCC 2 cut(s) 152, 500
PsuI RGATCY 1 cut(s) 217
SaqAI TTAA 6 cut(s) 492, 621, 642, 813, 825, 865
SatI GCNGC 2 cut(s) 165, 728
Sau3AI GATC 4 cut(s) 217, 405, 624, 760
Sau96I GGNCC 2 cut(s) 152, 500
ScrFI CCNGG 1 cut(s) 504
SduI GDGCHC 3 cut(s) 267, 659, 702
SfaNI GCATC 4 cut(s) 64, 92, 456, 528
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 2 cut(s) 152, 500
Sse9I AATT 5 cut(s) 47, 102, 381, 618, 690
SsiI CCGC 2 cut(s) 25, 498
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 502
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 4 cut(s) 92, 397, 492, 834
TaqI TCGA 2 cut(s) 86, 291
TasI AATT 5 cut(s) 47, 102, 381, 618, 690
TfiI GAWTC 1 cut(s) 564
Tru1I TTAA 6 cut(s) 492, 621, 642, 813, 825, 865
Tru9I TTAA 6 cut(s) 492, 621, 642, 813, 825, 865
TscAI CASTG 3 cut(s) 198, 634, 778
TseFI GTSAC 3 cut(s) 329, 458, 752
TseI GCWGC 2 cut(s) 164, 727
Tsp45I GTSAC 3 cut(s) 329, 458, 752
TspDTI ATGAA 5 cut(s) 32, 374, 387, 561, 600
TspGWI ACGGA 2 cut(s) 28, 477
TspRI CASTG 3 cut(s) 198, 634, 778
VpaK11BI GGWCC 2 cut(s) 152, 500
XapI RAATTY 1 cut(s) 381
XbaI TCTAGA 1 cut(s) 43
XceI RCATGY 2 cut(s) 81, 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XspI CTAG 1 cut(s) 44
ZraI GACGTC 1 cut(s) 90
Zsp2I ATGCAT 2 cut(s) 6, 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.