MD13G1220100.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000434
Physical Location & Seq
Forward (+)
21302531 .. 21303676
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1220100.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGCTCAGAAATTGAATACCCTGACAGTGTTTTTGTTCTTATTTGCAATATATGGTGCAAAGCCCGGGTCTGATATCACACAGGCTTTAACCAAGGCTTGGACTGATGCATGTGCATCACCAACGGCGAGTAAGGTTGTTGTTCCGAGCAGGACATACAAGTTAACAAAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCAATTGAGATTCAAGTTCAAGGTGAATTGCAAGCTCCAGTAGATGGTGGCAAACTTACCAAACCAGATACTTGGGTTGCTTTAGAGCATATTAATATGCTCACCATCTCCGGTAGTGGAACTTTTGATGGCCAAGGTGCACTTGCTTGGAAGTTAAATGATTGCAATAAAAACAAAGATTGCAAATCTATCGCCATTAATATGAGGTTCAACTTTGTTTTCAATTCCACAATGCAGGACATAACTTCTCTCGACAGCAAGAATTTCCCCGTCAACATTTTGGGGTGCCACGATGTCACATTCCAACATGTCAATGTCACAACACCTGAAGAGAGCATAAATACCGATGGAATCCATATCGGGCGTTCACCTGGAATCAACATTATTGATGCACACATTGGAACTGGGGATGATTGTGTTTCCATTGGCGATGGAAGCAAAGAAATCACCGTAACTAAGGTTACTTATGGACCAGGCCATGGCATAAGCGTTGGAAGTCTTGGAAGGTACCCGAATGAAGAACCTGTGGTTGGAATCAGGGTTAGGAACTGTACCTTGACTAATACATCCAACGGTGTGAGAATCAAGACATGGCATGCTTCTCTTTCAGATGGTATTGCATCAGATATGCACTTTGAAGATATTACAGTGGTCAACATCAGCAACCCTATCATCATAGACCAACAATATTTCCCATGGAATCAATGTAAACGGAACATTCGGTCGAAAGTGAAGATCAGCAACGTCAACTTCAAGAACATTAAAGGCTCATCTGTAACACCACTTGCAGTTCAAATTGTATGCAGCAGGGGCTTACTGTGTGAGAATGTGGAATTGTCTAACATTGATTTCACCTATAACGGAAACAAGGCCCTTTTACCTCTCAATGTTCTAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

366

Amino Acids

39.65

Weight (kDa)

8.26

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 38 - 356 1.9e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 705
AccB1I GGYRCC 2 cut(s) 483, 705
AccB7I CCANNNNNTGG 3 cut(s) 99, 242, 677
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 1 cut(s) 460
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 2 cut(s) 707, 751
AfiI CCNNNNNNNGG 4 cut(s) 99, 242, 677, 728
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 9 cut(s) 16, 181, 212, 218, 409, 421, 836, 952, 992
AjnI CCWGG 2 cut(s) 568, 670
AjuI GAANNNNNNNTTGG 2 cut(s) 711, 743
AluBI AGCT 1 cut(s) 233
AluI AGCT 1 cut(s) 233
Alw21I GWGCWC 1 cut(s) 340
Alw44I GTGCAC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 3 cut(s) 328, 673, 1068
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 1002
ApoI RAATTY 1 cut(s) 460
ArsI GACNNNNNNTTYG 4 cut(s) 16, 48, 53, 85
AseI ATTAAT 2 cut(s) 291, 396
Asp718I GGTACC 1 cut(s) 705
AspS9I GGNCC 3 cut(s) 185, 668, 1069
AsuC2I CCSGG 2 cut(s) 66, 67
AsuHPI GGTGA 6 cut(s) 111, 233, 292, 558, 637, 1042
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 2 cut(s) 185, 668
BaeGI GKGCMC 1 cut(s) 340
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BalI TGGCCA 1 cut(s) 330
BanI GGYRCC 2 cut(s) 483, 705
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 1 cut(s) 1014
BccI CCATC 6 cut(s) 236, 311, 320, 539, 623, 803
BceAI ACGGC 1 cut(s) 141
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 2 cut(s) 570, 672
BcnI CCSGG 2 cut(s) 66, 67
BisI GCNGC 1 cut(s) 1003
BlsI GCNGC 1 cut(s) 1004
Bme1390I CCNGG 4 cut(s) 66, 67, 570, 672
Bme18I GGWCC 2 cut(s) 185, 668
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 3 cut(s) 185, 668, 1069
BmiI GGNNCC 3 cut(s) 198, 485, 707
BmrFI CCNGG 4 cut(s) 66, 67, 570, 672
BmrI ACTGGG 1 cut(s) 612
BmsI GCATC 4 cut(s) 97, 125, 577, 827
BmuI ACTGGG 1 cut(s) 612
BpmI CTGGAG 1 cut(s) 219
BpuMI CCSGG 2 cut(s) 66, 67
BsaJI CCNNGG 5 cut(s) 65, 93, 331, 676, 893
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 242, 677, 728
Bse1I ACTGG 2 cut(s) 236, 607
BseBI CCWGG 2 cut(s) 570, 672
BseDI CCNNGG 5 cut(s) 65, 93, 331, 676, 893
BseGI GGATG 2 cut(s) 613, 764
BseLI CCNNNNNNNGG 4 cut(s) 99, 242, 677, 728
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 236, 607
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 1 cut(s) 1014
Bsh1285I CGRYCG 1 cut(s) 923
BshFI GGCC 3 cut(s) 330, 675, 1070
BshNI GGYRCC 2 cut(s) 483, 705
BsiEI CGRYCG 1 cut(s) 923
BsiHKAI GWGCWC 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 66, 309
BslI CCNNNNNNNGG 4 cut(s) 99, 242, 677, 728
BsnI GGCC 3 cut(s) 330, 675, 1070
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 1 cut(s) 933
Bsp19I CCATGG 2 cut(s) 676, 893
BspANI GGCC 3 cut(s) 330, 675, 1070
BspCNI CTCAG 1 cut(s) 19
BspLI GGNNCC 3 cut(s) 198, 485, 707
BspT107I GGYRCC 2 cut(s) 483, 705
BsrI ACTGG 2 cut(s) 236, 607
BssECI CCNNGG 5 cut(s) 65, 93, 331, 676, 893
BssMI GATC 1 cut(s) 933
BssT1I CCWWGG 4 cut(s) 93, 331, 676, 893
Bst2UI CCWGG 2 cut(s) 570, 672
Bst4CI ACNGT 6 cut(s) 28, 649, 749, 773, 847, 1017
Bst6I CTCTTC 1 cut(s) 522
BstC8I GCNNGC 2 cut(s) 231, 795
BstDEI CTNAG 2 cut(s) 6, 654
BstDSI CCRYGG 2 cut(s) 676, 893
BstF5I GGATG 2 cut(s) 613, 764
BstKTI GATC 1 cut(s) 936
BstMBI GATC 1 cut(s) 933
BstMCI CGRYCG 1 cut(s) 923
BstMWI GCNNNNNNNGC 2 cut(s) 633, 1008
BstNI CCWGG 2 cut(s) 570, 672
BstNSI RCATGY 3 cut(s) 114, 509, 797
BstSCI CCNGG 4 cut(s) 64, 65, 568, 670
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 1 cut(s) 1014
BstXI CCANNNNNNTGG 1 cut(s) 270
BsuRI GGCC 3 cut(s) 330, 675, 1070
BtgI CCRYGG 2 cut(s) 676, 893
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 2 cut(s) 613, 764
BtsIMutI CAGTG 2 cut(s) 33, 852
Cac8I GCNNGC 2 cut(s) 231, 795
Cfr13I GGNCC 3 cut(s) 185, 668, 1069
Cfr9I CCCGGG 1 cut(s) 65
Csp6I GTAC 2 cut(s) 706, 750
CviAII CATG 6 cut(s) 111, 506, 677, 789, 794, 894
CviQI GTAC 2 cut(s) 706, 750
DdeI CTNAG 2 cut(s) 6, 654
DpnI GATC 1 cut(s) 935
DpnII GATC 1 cut(s) 933
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 522
Eco130I CCWWGG 4 cut(s) 93, 331, 676, 893
Eco32I GATATC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 185, 668
Eco57I CTGAAG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 65
EcoO109I RGGNCCY 2 cut(s) 185, 1069
EcoRII CCWGG 2 cut(s) 568, 670
EcoRV GATATC 1 cut(s) 76
EcoT14I CCWWGG 4 cut(s) 93, 331, 676, 893
EcoT22I ATGCAT 1 cut(s) 112
ErhI CCWWGG 4 cut(s) 93, 331, 676, 893
FaeI CATG 6 cut(s) 114, 509, 680, 792, 797, 897
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FatI CATG 6 cut(s) 110, 505, 676, 788, 793, 893
Fnu4HI GCNGC 1 cut(s) 1003
FokI GGATG 2 cut(s) 620, 751
Fsp4HI GCNGC 1 cut(s) 1003
GluI GCNGC 1 cut(s) 1003
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 3 cut(s) 330, 675, 1070
HapII CCGG 2 cut(s) 66, 309
Hin1II CATG 6 cut(s) 114, 509, 680, 792, 797, 897
HincII GTYRAC 4 cut(s) 165, 472, 853, 946
HindII GTYRAC 4 cut(s) 165, 472, 853, 946
HinfI GANTC 6 cut(s) 208, 549, 573, 732, 780, 898
HpaI GTTAAC 1 cut(s) 165
HpaII CCGG 2 cut(s) 66, 309
HphI GGTGA 6 cut(s) 111, 233, 292, 558, 637, 1042
Hpy166II GTNNAC 7 cut(s) 165, 338, 472, 566, 853, 908, 946
Hpy188I TCNGA 5 cut(s) 9, 73, 147, 808, 823
Hpy188III TCNNGA 3 cut(s) 449, 784, 952
Hpy8I GTNNAC 7 cut(s) 165, 338, 472, 566, 853, 908, 946
HpyAV CCTTC 1 cut(s) 696
HpyCH4III ACNGT 6 cut(s) 28, 649, 749, 773, 847, 1017
HpyCH4IV ACGT 1 cut(s) 942
HpyF10VI GCNNNNNNNGC 2 cut(s) 633, 1008
HpyF3I CTNAG 2 cut(s) 6, 654
HpySE526I ACGT 1 cut(s) 942
Hsp92II CATG 6 cut(s) 114, 509, 680, 792, 797, 897
KpnI GGTACC 1 cut(s) 709
KspAI GTTAAC 1 cut(s) 165
Kzo9I GATC 1 cut(s) 933
LmnI GCTCC 2 cut(s) 202, 238
Lsp1109I GCAGC 1 cut(s) 1014
LweI GCATC 4 cut(s) 97, 125, 577, 827
MaeII ACGT 1 cut(s) 942
MaeIII GTNAC 5 cut(s) 493, 514, 649, 658, 973
MalI GATC 1 cut(s) 935
MboI GATC 1 cut(s) 933
MboII GAAGA 4 cut(s) 539, 728, 848, 943
MfeI CAATTG 1 cut(s) 201
MhlI GDGCHC 1 cut(s) 340
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 7 cut(s) 11, 201, 224, 421, 460, 993, 1031
MluNI TGGCCA 1 cut(s) 330
MmeI TCCRAC 4 cut(s) 526, 670, 709, 792
MnlI CCTC 2 cut(s) 396, 1089
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 1 cut(s) 112
MscI TGGCCA 1 cut(s) 330
MseI TTAA 6 cut(s) 89, 164, 291, 353, 396, 960
MslI CAYNNNNRTG 2 cut(s) 398, 510
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 2 cut(s) 66, 309
MspR9I CCNGG 4 cut(s) 66, 67, 570, 672
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 2 cut(s) 570, 672
MwoI GCNNNNNNNGC 2 cut(s) 633, 1008
NciI CCSGG 2 cut(s) 66, 67
NcoI CCATGG 2 cut(s) 676, 893
NdeII GATC 1 cut(s) 933
NlaIII CATG 6 cut(s) 114, 509, 680, 792, 797, 897
NlaIV GGNNCC 3 cut(s) 198, 485, 707
NmuCI GTSAC 2 cut(s) 493, 514
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 3 cut(s) 114, 509, 797
PaeI GCATGC 1 cut(s) 797
PciI ACATGT 1 cut(s) 505
PfeI GAWTC 6 cut(s) 208, 549, 573, 732, 780, 898
PflMI CCANNNNNTGG 3 cut(s) 99, 242, 677
PkrI GCNGC 1 cut(s) 1004
PpuMI RGGWCCY 1 cut(s) 185
PscI ACATGT 1 cut(s) 505
PshBI ATTAAT 2 cut(s) 291, 396
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 568, 670
PspGI CCWGG 2 cut(s) 568, 670
PspN4I GGNNCC 3 cut(s) 198, 485, 707
PspPI GGNCC 3 cut(s) 185, 668, 1069
PspPPI RGGWCCY 1 cut(s) 185
RsaI GTAC 2 cut(s) 707, 751
RsaNI GTAC 2 cut(s) 706, 750
RseI CAYNNNNRTG 2 cut(s) 398, 510
SaqAI TTAA 6 cut(s) 89, 164, 291, 353, 396, 960
SatI GCNGC 1 cut(s) 1003
Sau3AI GATC 1 cut(s) 933
Sau96I GGNCC 3 cut(s) 185, 668, 1069
ScrFI CCNGG 4 cut(s) 66, 67, 570, 672
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 4 cut(s) 97, 125, 577, 827
SinI GGWCC 2 cut(s) 185, 668
SmaI CCCGGG 1 cut(s) 67
SmiMI CAYNNNNRTG 2 cut(s) 398, 510
SphI GCATGC 1 cut(s) 797
Sse9I AATT 7 cut(s) 11, 201, 224, 421, 460, 993, 1031
SspI AATATT 1 cut(s) 887
StyD4I CCNGG 4 cut(s) 64, 65, 568, 670
StyI CCWWGG 4 cut(s) 93, 331, 676, 893
TaaI ACNGT 6 cut(s) 28, 649, 749, 773, 847, 1017
TaiI ACGT 1 cut(s) 945
TaqI TCGA 2 cut(s) 450, 923
TaqII GACCGA 1 cut(s) 909
TasI AATT 7 cut(s) 11, 201, 224, 421, 460, 993, 1031
TfiI GAWTC 6 cut(s) 208, 549, 573, 732, 780, 898
Tru1I TTAA 6 cut(s) 89, 164, 291, 353, 396, 960
Tru9I TTAA 6 cut(s) 89, 164, 291, 353, 396, 960
TscAI CASTG 2 cut(s) 33, 852
TseFI GTSAC 2 cut(s) 493, 514
TseI GCWGC 1 cut(s) 1002
Tsp45I GTSAC 2 cut(s) 493, 514
TspDTI ATGAA 1 cut(s) 729
TspGWI ACGGA 2 cut(s) 925, 1074
TspMI CCCGGG 1 cut(s) 65
TspRI CASTG 2 cut(s) 33, 852
Van91I CCANNNNNTGG 3 cut(s) 99, 242, 677
VneI GTGCAC 1 cut(s) 336
VpaK11BI GGWCC 2 cut(s) 185, 668
VspI ATTAAT 2 cut(s) 291, 396
XapI RAATTY 1 cut(s) 460
XceI RCATGY 3 cut(s) 114, 509, 797
XmaI CCCGGG 1 cut(s) 65
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.