Rroxscaffold_2G00090810

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000434
Physical Location & Seq
Reverse (-)
12544225 .. 12545409
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090810.1

Sequence Viewer

Length: 1185 bp
ATGGGTCTCAAATTGATGACTATCATGGCGATATTCATTATCTTACTGTTAGCCCAGCCCCGTGTAGTCTTTGACGTGACGAGTGCAAAATATGGTGGAAAAACTAACTATGATATTACAGACTCCTTAGCACAAGCTTGGACAGATGCTTGTGCTTCTGTATGGCCGAGTAAACTCGTTGTTCCAAACGGCACATATTGGTTGAGAGGAGCAACTTTTATAGGTCCTTGCAAGGCTCCTATCGAGGTGCAAATCCAAGGAAAGTTGAAAGCTCCCGAAGATGGCCACCAACTCGTGAACTCGGATTTTTGGGTTGCTTTTCGGTACATTGACAGGCTCTACTTATCAGGTGGTGGAACTTTTGATGGCCAAGGAGCCCTTGATTGGAATCCTTATCGTGCTATTAACGTGAGATTCGACTACATCTCCAACTCCATAATTCAGGACATAACTTCCCTTAACAGCAGAAGTTTCCACATTAACGTTTACGCGTACCATGATGTTACATTCCAGCGTGTGACTGTCATAGCACCCGGCGAGAGTTCTAATACGGATGGAATCCACATTGCACGTTCATGGAGGATCAACGTAACGGATACAAACATCGGAACCGGAGATGGTTGTATTTCCATTGGTGATGCCACTTCGCAACTCATAGTAACCAATGTTAATTGTGGACCGGGACATGGAATAAGCGTAGGAAGTCTTGGAAAGTATCTTGATGAAAAACCGGTATATGGCCTCATCATTAAGAACTGCACCCTCACGGACACAACAAACGGTGTGAGAATCAAGACATGGCCTGGTTCTCACAGTGCCGGTTTTGCCTCACACTTATACTTTGAGGATATTATCATGAGTAATGTGAAGAACCCCATCATTATAGACCAAAACTACTGCCCCGGGCATAGTTGCAAACAACAGGCTCAGTCGAACGTTAAGATTAGTAATGTCAGCTTCAAGAACATCAGAGGCTCATCTGCATCACAAGTTGCAGTCAATCTTGCGTGTAGTGGGAGCTTACCATGTGAGAATGTAGAACTGATCATAGGCATTGATCTCGTATTCAGTGGAGATGGCGATCGACCTATTACATCTCGATGCTCTAATGTCATCAAGCCCACAATTATTAAAGTGAGTGAGGCTCTTGCTAGCTTGCAGACCCTACCCAATAAATCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

394

Amino Acids

42.77

Weight (kDa)

7.05

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 51 - 372 1.3e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 491
AclI AACGTT 2 cut(s) 483, 936
AclWI GGATC 1 cut(s) 590
AcoI YGGCCR 3 cut(s) 164, 283, 367
AfaI GTAC 2 cut(s) 326, 494
AfiI CCNNNNNNNGG 4 cut(s) 281, 384, 686, 737
AflIII ACRYGT 1 cut(s) 489
AgeI ACCGGT 1 cut(s) 730
AgsI TTSAA 2 cut(s) 268, 961
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 802
AluBI AGCT 5 cut(s) 137, 272, 957, 1020, 1155
AluI AGCT 5 cut(s) 137, 272, 957, 1020, 1155
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 1 cut(s) 590
Ama87I CYCGRG 1 cut(s) 902
AoxI GGCC 5 cut(s) 164, 283, 367, 739, 800
AsiGI ACCGGT 1 cut(s) 730
AspS9I GGNCC 2 cut(s) 224, 677
AsuC2I CCSGG 4 cut(s) 534, 681, 903, 904
AsuHPI GGTGA 1 cut(s) 647
AsuNHI GCTAGC 1 cut(s) 1151
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 2 cut(s) 224, 677
BalI TGGCCA 2 cut(s) 285, 369
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 1 cut(s) 293
BccI CCATC 6 cut(s) 275, 359, 548, 611, 884, 1070
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 1042, 1076
BciT130I CCWGG 1 cut(s) 804
BciVI GTATCC 1 cut(s) 589
BclI TGATCA 1 cut(s) 1044
BcnI CCSGG 4 cut(s) 534, 681, 903, 904
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 1 cut(s) 1152
BfuI GTATCC 1 cut(s) 589
Bme1390I CCNGG 5 cut(s) 534, 681, 804, 903, 904
Bme18I GGWCC 2 cut(s) 224, 677
BmeT110I CYCGRG 1 cut(s) 902
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 224, 677
BmiI GGNNCC 3 cut(s) 237, 376, 610
BmrFI CCNGG 5 cut(s) 534, 681, 804, 903, 904
BmsI GCATC 4 cut(s) 136, 628, 992, 1091
BmtI GCTAGC 1 cut(s) 1155
BplI GAGNNNNNCTC 2 cut(s) 1129, 1161
Bpu10I CCTNAGC 1 cut(s) 127
BpuMI CCSGG 4 cut(s) 534, 681, 903, 904
BsaBI GATNNNNATC 3 cut(s) 20, 387, 1080
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 256, 370, 901, 902
BsaWI WCCGGW 2 cut(s) 611, 730
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 384, 686, 737
Bse118I RCCGGY 2 cut(s) 730, 818
Bse3DI GCAATG 1 cut(s) 564
Bse8I GATNNNNATC 3 cut(s) 20, 387, 1080
BseBI CCWGG 1 cut(s) 804
BseDI CCNNGG 4 cut(s) 256, 370, 901, 902
BseGI GGATG 1 cut(s) 559
BseJI GATNNNNATC 3 cut(s) 20, 387, 1080
BseLI CCNNNNNNNGG 4 cut(s) 281, 384, 686, 737
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 1 cut(s) 941
BseRI GAGGAG 1 cut(s) 222
BseYI CCCAGC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 1084
BshFI GGCC 5 cut(s) 166, 285, 369, 741, 802
BshTI ACCGGT 1 cut(s) 730
BsiEI CGRYCG 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 6 cut(s) 534, 612, 680, 731, 819, 903
BslFI GGGAC 1 cut(s) 696
BslI CCNNNNNNNGG 4 cut(s) 281, 384, 686, 737
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 696
BsnI GGCC 5 cut(s) 166, 285, 369, 741, 802
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 902
Bsp1286I GDGCHC 1 cut(s) 379
Bsp143I GATC 4 cut(s) 582, 1044, 1057, 1081
BspANI GGCC 5 cut(s) 166, 285, 369, 741, 802
BspCNI CTCAG 1 cut(s) 940
BspFNI CGCG 1 cut(s) 491
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 3 cut(s) 237, 376, 610
BspOI GCTAGC 1 cut(s) 1155
BspPI GGATC 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 564
BsrFI RCCGGY 2 cut(s) 730, 818
BssAI RCCGGY 2 cut(s) 730, 818
BssECI CCNNGG 4 cut(s) 256, 370, 901, 902
BssMI GATC 4 cut(s) 582, 1044, 1057, 1081
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 2 cut(s) 256, 370
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 804
Bst4CI ACNGT 4 cut(s) 48, 523, 782, 815
BstC8I GCNNGC 2 cut(s) 1153, 1157
BstDEI CTNAG 2 cut(s) 127, 927
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 1 cut(s) 491
BstKTI GATC 4 cut(s) 585, 1047, 1060, 1084
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 4 cut(s) 582, 1044, 1057, 1081
BstMCI CGRYCG 1 cut(s) 1084
BstMWI GCNNNNNNNGC 1 cut(s) 824
BstNI CCWGG 1 cut(s) 804
BstSCI CCNGG 5 cut(s) 532, 679, 802, 901, 902
BstUI CGCG 1 cut(s) 491
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 5 cut(s) 166, 285, 369, 741, 802
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 559
BtsIMutI CAGTG 2 cut(s) 820, 1075
Cac8I GCNNGC 2 cut(s) 1153, 1157
CciI TCATGA 1 cut(s) 855
Cfr10I RCCGGY 2 cut(s) 730, 818
Cfr13I GGNCC 2 cut(s) 224, 677
Cfr9I CCCGGG 1 cut(s) 902
Csp6I GTAC 2 cut(s) 325, 493
CspAI ACCGGT 1 cut(s) 730
CviAII CATG 7 cut(s) 25, 497, 576, 686, 798, 856, 1026
CviQI GTAC 2 cut(s) 325, 493
DdeI CTNAG 2 cut(s) 127, 927
DpnI GATC 4 cut(s) 584, 1046, 1059, 1083
DpnII GATC 4 cut(s) 582, 1044, 1057, 1081
EaeI YGGCCR 3 cut(s) 164, 283, 367
Eco130I CCWWGG 2 cut(s) 256, 370
Eco24I GRGCYC 1 cut(s) 379
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 224, 677
Eco88I CYCGRG 1 cut(s) 902
EcoO109I RGGNCCY 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 802
EcoT14I CCWWGG 2 cut(s) 256, 370
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 256, 370
FaeI CATG 7 cut(s) 28, 500, 579, 689, 801, 859, 1029
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 1 cut(s) 696
FatI CATG 7 cut(s) 24, 496, 575, 685, 797, 855, 1025
FbaI TGATCA 1 cut(s) 1044
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 379
FspBI CTAG 1 cut(s) 1152
GsaI CCCAGC 1 cut(s) 58
HaeIII GGCC 5 cut(s) 166, 285, 369, 741, 802
HapII CCGG 6 cut(s) 534, 612, 680, 731, 819, 903
Hin1II CATG 7 cut(s) 28, 500, 579, 689, 801, 859, 1029
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 5 cut(s) 122, 388, 414, 558, 789
HpaII CCGG 6 cut(s) 534, 612, 680, 731, 819, 903
HphI GGTGA 1 cut(s) 647
Hpy166II GTNNAC 4 cut(s) 173, 298, 487, 677
Hpy188I TCNGA 4 cut(s) 304, 608, 971, 1180
Hpy188III TCNNGA 8 cut(s) 275, 295, 443, 719, 793, 856, 961, 1098
Hpy8I GTNNAC 4 cut(s) 173, 298, 487, 677
HpyCH4III ACNGT 4 cut(s) 48, 523, 782, 815
HpyCH4IV ACGT 6 cut(s) 75, 408, 483, 571, 588, 936
HpyCH4V TGCA 9 cut(s) 86, 231, 250, 569, 759, 915, 983, 995, 1159
HpyF10VI GCNNNNNNNGC 1 cut(s) 824
HpyF3I CTNAG 2 cut(s) 127, 927
HpySE526I ACGT 6 cut(s) 75, 408, 483, 571, 588, 936
Hsp92II CATG 7 cut(s) 28, 500, 579, 689, 801, 859, 1029
Ksp22I TGATCA 1 cut(s) 1044
Kzo9I GATC 4 cut(s) 582, 1044, 1057, 1081
LmnI GCTCC 5 cut(s) 209, 241, 277, 374, 1017
LweI GCATC 4 cut(s) 136, 628, 992, 1091
MaeI CTAG 1 cut(s) 1152
MaeII ACGT 6 cut(s) 75, 408, 483, 571, 588, 936
MaeIII GTNAC 5 cut(s) 76, 502, 517, 589, 658
MalI GATC 4 cut(s) 584, 1046, 1059, 1083
MboI GATC 4 cut(s) 582, 1044, 1057, 1081
MboII GAAGA 2 cut(s) 290, 880
MhlI GDGCHC 1 cut(s) 379
MlsI TGGCCA 2 cut(s) 285, 369
MluCI AATT 4 cut(s) 11, 438, 670, 1125
MluI ACGCGT 1 cut(s) 489
MluNI TGGCCA 2 cut(s) 285, 369
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 453
MnlI CCTC 9 cut(s) 200, 238, 573, 752, 773, 838, 838, 965, 1135
Mox20I TGGCCA 2 cut(s) 285, 369
MscI TGGCCA 2 cut(s) 285, 369
MseI TTAA 7 cut(s) 405, 459, 480, 669, 750, 939, 1131
MslI CAYNNNNRTG 2 cut(s) 574, 1099
Msp20I TGGCCA 2 cut(s) 285, 369
MspI CCGG 6 cut(s) 534, 612, 680, 731, 819, 903
MspR9I CCNGG 5 cut(s) 534, 681, 804, 903, 904
MvaI CCWGG 1 cut(s) 804
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 1 cut(s) 824
NciI CCSGG 4 cut(s) 534, 681, 903, 904
NdeII GATC 4 cut(s) 582, 1044, 1057, 1081
NheI GCTAGC 1 cut(s) 1151
NlaIII CATG 7 cut(s) 28, 500, 579, 689, 801, 859, 1029
NlaIV GGNNCC 3 cut(s) 237, 376, 610
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 76, 517
PagI TCATGA 1 cut(s) 855
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 4 cut(s) 388, 414, 558, 789
PinAI ACCGGT 1 cut(s) 730
Ple19I CGATCG 1 cut(s) 1084
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PpuMI RGGWCCY 1 cut(s) 224
Psp1406I AACGTT 2 cut(s) 483, 936
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 802
PspFI CCCAGC 1 cut(s) 54
PspGI CCWGG 1 cut(s) 802
PspN4I GGNNCC 3 cut(s) 237, 376, 610
PspPI GGNCC 2 cut(s) 224, 677
PspPPI RGGWCCY 1 cut(s) 224
PvuI CGATCG 1 cut(s) 1084
RsaI GTAC 2 cut(s) 326, 494
RsaNI GTAC 2 cut(s) 325, 493
RseI CAYNNNNRTG 2 cut(s) 574, 1099
SaqAI TTAA 7 cut(s) 405, 459, 480, 669, 750, 939, 1131
Sau3AI GATC 4 cut(s) 582, 1044, 1057, 1081
Sau96I GGNCC 2 cut(s) 224, 677
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 5 cut(s) 534, 681, 804, 903, 904
SduI GDGCHC 1 cut(s) 379
SfaNI GCATC 4 cut(s) 136, 628, 992, 1091
SinI GGWCC 2 cut(s) 224, 677
SmaI CCCGGG 1 cut(s) 904
SmiMI CAYNNNNRTG 2 cut(s) 574, 1099
Sse9I AATT 4 cut(s) 11, 438, 670, 1125
SspMI CTAG 1 cut(s) 1152
StyD4I CCNGG 5 cut(s) 532, 679, 802, 901, 902
StyI CCWWGG 2 cut(s) 256, 370
TaaI ACNGT 4 cut(s) 48, 523, 782, 815
TaiI ACGT 6 cut(s) 78, 411, 486, 574, 591, 939
TaqI TCGA 5 cut(s) 243, 417, 932, 1084, 1099
TasI AATT 4 cut(s) 11, 438, 670, 1125
TfiI GAWTC 4 cut(s) 388, 414, 558, 789
Tru1I TTAA 7 cut(s) 405, 459, 480, 669, 750, 939, 1131
Tru9I TTAA 7 cut(s) 405, 459, 480, 669, 750, 939, 1131
TscAI CASTG 2 cut(s) 820, 1075
TseFI GTSAC 2 cut(s) 76, 517
Tsp45I GTSAC 2 cut(s) 76, 517
TspDTI ATGAA 3 cut(s) 25, 564, 738
TspGWI ACGGA 3 cut(s) 566, 608, 782
TspMI CCCGGG 1 cut(s) 902
TspRI CASTG 2 cut(s) 820, 1075
VpaK11BI GGWCC 2 cut(s) 224, 677
XmaI CCCGGG 1 cut(s) 902
XspI CTAG 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.